the clinical significance of nuclear yap expression in hepatocellular carcinoma

1

The Clinical Significance of Nuclear YAP Expression

in Hepatocellular Carcinoma

徐 智南京医科大学附属南京第一医院暨

南京市第一医院 肿瘤内科

Worldwide incidence of HCC

Blum HE, Spangenberg HC. Arch Iran Med. 2007 Jul;10(3):361-71.

Identification of Yes-associated Protein (YAP)

Zender, L. et al. Cell 125, 1253-67

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F

G

H

YAP improves cell proliferation

Time (Days)

* ： p<0.05 。

YAP induces anchorage-independent growth

MIHA-YAP cells but not MIHA-Vec could form colonies in soft-agar.

MIHA-YAP cells gained advantages in cell migration and invasion.

YAP enhances cellular mobility and invasiveness

IHC results of YAP in HCC

53.7% HCC samples showed nuclear YAP positive.

Clinical association of nuclear YAP in HCC

Clinical association of nuclear YAP and HCC-specific postsurgical recurrence

Kaplan-Meier

Median RFT ：Nuclear YAP negative: 25.8 months vs. Nuclear YAP positive ： 12.9 months

Nuclear YAP is an independent prognostic marker for HCC recurrence

Cox Regression

Summary• YAP confers tumorigenic and metastatic potentials in

hepatocytes, as evidenced by enhanced aptitudes in cell viability, anchorage-independent growth, migration and invasion.

• YAP nuclear accumulation was found in the 53.7% of HCC, correlated with poorer tumor differentiation and higher serum alpha-fetoprotein (AFP) levels.

• Nuclear YAP is an independent predictor for HCC-specific recurrence-free survival.

• YAP plays an important role in HCC development and progression.

Acknowledgement

新加坡国立大学外科系和药学系 Asso. Prof. John M. Luk

香港大学外科系 Prof. Fan S. T. ， Prof. Poon R. T. and

Surgical Tissue Bank

南京市第一医院肿瘤内科