ttr-rb15
Post on 30-Dec-2015
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(A). (B). pAAV2-TTR-RB15 (used as marker). scAAV2-TTR-RB15. 3’. 5’. TTR-RB15. TTR-RB15 monomer, 2490 nt. Not-digested. Dimer, 4856 nt. Parent, 3967 nt. Monomer, 2490 nt. 3’. TTR-RB15. 5’. Self-complementary TTR-RB15 dimer, 4856 nt. RT-PCR. (C). PCR. scRB15- mutant. scRB15- - PowerPoint PPT Presentation
TRANSCRIPT
5’3’TTR-RB15
TTR-RB15 monomer, 2490 nt
TTR-RB155’3’
Self-complementaryTTR-RB15 dimer, 4856 nt
(A) pAAV2-TTR-RB15(used as marker) scAAV2-TTR-RB15
Dimer, 4856 nt
Monomer, 2490 ntParent, 3967 nt
Not-digested
(B)
days 1 1 1 13 3 3 3
HepG2
Parent sc
RB15scR
B15-
mutant
RT-PCR PCR
1 1 1 13 3 3 3
HepG2
Parent sc
RB15scR
B15-
mutant
RB15
(C)
(D)
Apo
B m
RN
A in
Hep
G2
(% o
f in
acti
ve R
B15
-mut
ant)
Ad- RB1 5
-mutant
Ad-RB15
Parent
- AAV2-TTR
-RB1 5-mut ant
scAAV2-TTR
-RB15
scAAV2-TTR
-RB15-mutant
0
25
50
75
100
125
Parent-AAV2
-TTR-RB15
P=0.005 P=0.001ns
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