whey pilot test case study (with ph adjustment and

Whey Pilot Test Case Study(with pH adjustment and bioaugmentation)

Shuron NPL SiteBarnwell CountyPartial Success

Shuron Site Map

General Location of Study Area

MP-1

MP-5

(The Good)

(The Bad)

pH adjust

BioaugmentationBioaugmentation

Injection DetailsPhase 1– 30 injection points– 17,787 Gallons Water– 8,400 Lbs Whey

Phase 2– 30 injection points– 7967 Gallons Water– 2080 Lbs Whey– 0.015 molar sodium

bicarbonate - 9 points– Bioaugmentation 620

gallons - 3 points

The Good

Shuron MP-1

0

50

100

150

200

250

0 1 2 3 4 5 6 7

pH

viny

l chl

orid

e (u

g/l)

Shuron MP-1

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7

pH

Ethe

ne (u

g/l)

pH=4.29 pH=4.1 pH=4.17 pH=4.8

MP-5 Water Quality

Shuron Site Dissolved Organic Carbon

Shuron Dehalococcoides Population MP-5

1.00E+00

1.00E+01

1.00E+02

1.00E+03

1.00E+04

1.00E+05

1.00E+06

1.00E+07

1.00E+08

1.00E+09

7/14/2007 9/2/2007 10/22/2007 12/11/2007 1/30/2008 3/20/2008

Date

Gen

e C

opie

s/Li

ter

16S RNAbvc-Avcr-A

4.17

4.72

4.80

Shuron NPL Site: Observations/Lessons Learned

Success at increasing DOC in wellsAquifer buffering capacity rendered pH adjustment attempts unsuccessfulBioaugmentation unsuccessful because of low pH/aquifer bufferingBiofouling reduced injection effectivenessSuccessful where pH = 6 or aboveDid not work in areas of reduced pH