www.wilksir.com infracal tog/tph analyzer, model cvh extraction procedure

www.wilksIR.comwww.wilksIR.com

InfraCal TOG/TPH Analyzer,InfraCal TOG/TPH Analyzer,Model CVHModel CVH

Extraction ProcedureExtraction Procedure

The sample extraction can be done The sample extraction can be done using either a prescription bottle using either a prescription bottle

with a with a

septa cap or a separatory funnelsepta cap or a separatory funnel

Extraction using a prescription Extraction using a prescription bottle with a septa capbottle with a septa cap

Collect a measured amount of Collect a measured amount of samplesample

For samples that will be stored for For samples that will be stored for more than a day or for samples more than a day or for samples with a high pH:with a high pH:

Adjust the pH to less than 2 with Adjust the pH to less than 2 with Hydrochloric acid or Sulfuric Acid Hydrochloric acid or Sulfuric Acid (typically 3-5 drops depending (typically 3-5 drops depending on buffers in sample)on buffers in sample)

Add SolventAdd Solvent

10 parts sample to 1 part solvent

For example:For example:

For 150 ml of sample addFor 150 ml of sample add

15 ml of solvent 15 ml of solvent

Shake for 2 minutesShake for 2 minutes

Open the cap on the bottle during Open the cap on the bottle during the 2 minute shaking to relieve the 2 minute shaking to relieve the pressure the pressure

Solvent is heavier than water and will Solvent is heavier than water and will sinksink

Turn bottle upside down Turn bottle upside down

Use the syringe to remove the solvent Use the syringe to remove the solvent

Fill the cuvette with solvent Fill the cuvette with solvent

Place the cuvette in the InfraCal Place the cuvette in the InfraCal frosted side to the front frosted side to the front

Press the run button Press the run button

Oil concentration displayed Oil concentration displayed

14

Extraction using a separatory funnelExtraction using a separatory funnel

Pour measured sample into Pour measured sample into separatory funnelseparatory funnel

Measure one tenth of the sample size Measure one tenth of the sample size of solventof solvent

For example:For example:

For 150 ml of sample addFor 150 ml of sample add

15 ml of solvent 15 ml of solvent

Pour the solvent into the sample Pour the solvent into the sample collection container to rinse interior collection container to rinse interior surfaces and cap of left over oilsurfaces and cap of left over oil

Pour the solvent from the sample Pour the solvent from the sample container into separatory funnel container into separatory funnel containing samplecontaining sample

Shake for 2 minutes Shake for 2 minutes

Stop to vent during the 2 minutes Stop to vent during the 2 minutes shaking to relieve the pressure shaking to relieve the pressure

Put the separatory funnel into Put the separatory funnel into the stand the stand

Allow the solvent to sink to the Allow the solvent to sink to the bottom bottom

Fill the cuvette with solvent Fill the cuvette with solvent

Place the cuvette in the InfraCal Place the cuvette in the InfraCal frosted side to the front frosted side to the front

Press the run button Press the run button

Oil concentration displayed Oil concentration displayed

14

Silica Gel ExtractionSilica Gel Extraction

The difference between TPH (Total The difference between TPH (Total Petroleum Hydrocarbon) and TOG Petroleum Hydrocarbon) and TOG (Total Oil and Grease) is the polar (Total Oil and Grease) is the polar

organics are removed from the extract organics are removed from the extract using silica gel. The remaining using silica gel. The remaining hydrocarbons are the non-polar hydrocarbons are the non-polar

components considered to be TPHcomponents considered to be TPH

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica Put funnel into bottlePut funnel into bottle Put solvent extract throughPut solvent extract through

silica gelsilica gel

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica Put funnel into bottlePut funnel into bottle Put solvent extract throughPut solvent extract through

silica gelsilica gel

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica Put funnel into bottlePut funnel into bottle Put solvent extract throughPut solvent extract through

silica gelsilica gel Put solvent extract into cuvettePut solvent extract into cuvette

Silica Gel ExtractionSilica Gel Extraction

Put filter paper into funnelPut filter paper into funnel Add silica Add silica Put funnel into bottlePut funnel into bottle Put solvent extract throughPut solvent extract through

silica gelsilica gel Put solvent extract into cuvettePut solvent extract into cuvette Put cuvette into InfraCal frosted Put cuvette into InfraCal frosted

side to the frontside to the front

Press the run button Press the run button

Oil concentration displayed Oil concentration displayed

14

For questions please contact us at:For questions please contact us at:tech@WilksIR.com, or 203-855-9136tech@WilksIR.com, or 203-855-9136

InfraCal TOG/TPH Analyzer,InfraCal TOG/TPH Analyzer,Model CVHModel CVH

Extraction ProcedureExtraction Procedure