Supporting Information

An insight into the optical property of sub nano

size glutathione stabilized gold cluster

Lakshmi V Nair, Resmi V Nair, Ramapurath S Jayasree*

Division of Biophotonics and Imaging, SreeChitraTirunal Institute for Medical Sciences and

Technology, Biomedical Technology Wing, Trivandrum 695012, India.

 

 

 

Fig. S1 (a)Absorbance, (b) fluorescence at different excitation and (c) TEM of GMSA

400 600 8000.0

0.5

1.0

A

/ nm

GMSA

a) b)

c)

350 400 450 500 550 6000.0

0.5

1.0

I/ a

.u.

GMSA 280 GMSA 300 GMSA 320 GMSA 350 GMSA 380 GMSA 400 GMSA 450

/ nm

Electronic Supplementary Material (ESI) for Dalton Transactions.This journal is © The Royal Society of Chemistry 2016

Name of the cluster

pH Temperature Emission @ 450 nm excitation (nm)

(λmax) 750/(λmax)530 @ 450 nm excitation

GSH Reaction @ 12 h

AuC1 1.5 27o 27o 750 1.2

AuC2 1.5 27o 70o 750 2.5

AuC3 1.5 0o 70o 750 50.42

AuC4 10 27o 70o

Table S1: Comparison of reaction condition on fluorescence property

Fig.S2 Lower magnification TEM image of AuC1, AuC2 and AuC4. All the scale bars are 50 nm

AuC1 AuC2 AuC4

Fig. S3 Size distribution of the cluster from TEM.

Fig.S4 UV- Visible absorbance spectra of developed nano materials

0.6 0.7 0.8 0.9 1.0

0

10

20

30

40

50

60

AuC3

Pe

rce

ntag

e / %

d/ nm

0.6 0.9 1.2 1.5 1.8 2.1 2.40

10

20

30

Pe

rce

ntag

e / %

d/ nm

AuC1

0.6 0.8 1.0 1.2 1.40

10

20

30

40

AuC2

Pe

rce

ntag

e / %

d/ nm

1.5 1.6 1.7 1.8 1.9 2.0 2.10

10

20

30

40

50

AuC4

Pe

rce

ntag

e / %

d/ nm

a)

c)

b)

d)

300 400 500 600 700 8000.0

0.5

1.0

A

GMSA AuC1 AuC2 AuC3 AuC4

/ nm

 

 

 

 

Fig. S5XPS spectra of the developed clusters

 

 

 

 

 

 

 

 

 

Fig. S6 Raman Spectra of AuC1, AuC2, AuC3 and AuC4 , Individual and magnified spectra of AuC1,AuC2,AuC3 and AuC4 in the range 1000 to 1700 cm-1 are shown in Fig S6a-d

 

 

 

 

 

 

 

S6a

80 82 84 86 88 90 920

50

100

I/ a.

u.

B.E/ eV

AuC1 AuC2 AuC3 AuC4

600 900 1200 1500 1800 2100 2400 2700

14001500

1600

500 1000 1500 2000 2500

1500

2000

1000 1500 2000 2500

1500

1000 1500 2000 25001000

1500

2000

AuC1

Raman Shift (cm-1)

Inte

nsi

ty (

AU

)

AuC2

AuC3

AuC4

1300 1400 1500 1600 17001440

1500

1560

Inte

nsi

ty(A

U)

Raman Shift ( cm-1)

AuC1

13841432

1629

S6b

S6c

1300 1400 1500 1600 1700

1900

1920

1940

1960

1980

2000

2020

2040

Inte

nsi

ty(A

U)

Raman Shift ( cm-1)

AuC2

13841418

1627

1000 1200 1400 16001150

1200

1250

1300

1350

1400

Inte

nsi

ty(A

U)

Raman Shift ( cm-1)

AuC3

1099

1640

 

Fig. S7 Optimized geometry of self assembled structures, AuC1 (a), AuC2 (b), AuC3 (c) and AuC4 (d). In all the cases 4 repeated units are shown. Au, S, C, O and N are represented in brown, orange, yellow, red and blue respectively.

S6d

1300 1400 1500 1600 17001510

1520

1530

1540

1550

1560

1570

1580

Inte

nsi

ty(A

U)

Raman Shift ( cm-1)

AuC4

1385

1413

1613

Fig. S8 Optimized geometry of self assembled structures AuC4 showing the pattern similar to the one observed in the TEM image. Note the central gap.

Fig.S9 CT image of the developed cluster

AuC1 AuC3 AuC2 AuC4Blank

Fig. S10:O

 

Fig. S11:Inext day s

 

Optical imagi

In vivo opticashown along w

ing of the dev

al imaging potwith the color

veloped cluste

tential of AuCr coded effici

ers shown alo

C3 in mouse aiency bar.

ong with the c

and its cleara

color coded ef

ance from the

fficiency bar.

e body on the