an introduction to: dionex chromeleon 7 chromatography...
TRANSCRIPT
An Introduction to:
Thermo Scientific Dionex Chromeleon 7
Chromatography Data SystemChromatography Data System
• Introducing
• User Interface
• Peak Detection Tools
• eWorkflows™
• Interactive Charting
• Data Mining
• Reporting
Agenda
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• Reporting
• Intelligent Run Control
• Fraction Collection
• Mass Spectrometry
• Support for Third Party Instrumentation
• Networking Capabilities
• Summary
Introducing Chromeleon 7 Chromatography Data System
• New-generation Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System (CDS)
• “Simply Intelligent”
• Intelligent Functionality - it does everything you need!
• Operational Simplicity™ - everything is fast and easy!
• Simplifies all chromatography processes
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Operational Simplicity
• Operational Simplicity was the core design principle
• Major design rules:
• Minimize number of steps needed to perform any task
• Make all steps easy to understand and easy to use
• Minimize time needed to perform any task
• Major features for Operational Simplicity:
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• Modern user interface
• Peak detection and integration tools
• eWorkflows
• Data visualization tools
• Enterprise functionality
User Interface
• The Chromeleon CDS User Interface consists of two major parts:
• Chromeleon CDS Console
• Designed to provide easy navigation through your data
• Chromatography Studio
• Used for All Data Evaluation
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Console – Easy Access to Instruments via ePanels
Provides access to
all instruments on
the network
Immediate access
to Audit Trail
and Queue
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ePanels are auto-generated for each instrument.
the network
Peak Detection
• Most time consuming and tedious task in chromatography process
• Chromatogram Integration
• Chromeleon 7 CDS introduces two new tools to simplify this process
• Cobra™ Peak Detection Algorithm / Wizard
• SmartPeaks™ Integration Assistant
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Peak Detection – Cobra
• Cobra is a new algorithm that:
• Finds peak start and end times accurately and consistently
• Requires minimum number of parameters to reach integration goals
• Cobra Wizard provides:
• Fast, guided way to determine initial detection parameters
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Peak Detection - SmartPeaks
• Unique tool for easily reaching your integration goals for unresolved peaks
• Requires no training
• Great example of Operational Simplicity
• Process is clear and understandable
• Requires only a few steps
• Correctly integrates all unresolved peaks in seconds
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SmartPeaks – A Revolution in Ease of Use
• There are many ways to integrate unresolved peaks
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Is this what
we want?
Is this what
we want?
SmartPeaks Integration Assistant
1. Activate SmartPeaks Wizard
2. Select Area of Interest 3. Select the Integration You Want
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eWorkflows – From Samples to Results
• eWorkflows™ take you from Sample to Results in the minimum number of steps
• Fully customizable for any application
• Minimum amount of training required
• Reduces errors and produces results faster
• A new framework for Operational Simplicity
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eWorkflow for a Regulated USP/EP Method
3. Sequence is created and
can be immediately run
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1. Choose
eWorkflow, Select Instrument, and
Click “Launch”2. Enter Number of
Samples and Start Position
eWorkflow for a Regulated USP/EP Method
This eWorkflow also:
• Processes all data
• Calculates all results
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From Sample to Result in the Shortest Time Possible
eWorkflows
• eWorkflows to boost lab productivity - typically 30-50% time savings
• Applies to all laboratories, for example:
• QA/QC laboratories
• Method development laboratories
• Research & Development laboratories
• Walk-up instrument workflows
• Built-in Templates for Key Pharma Applications
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• Built-in Templates for Key Pharma Applications
• GPC
• ICH Method Validation
• Dissolution Testing
• Content Uniformity Testing
Data Visualization
• Interactive Charts for Data Visualization
• Fully customizeable
• Templates for the most common tasks
• Available for both on-screenand printed/exported reports.
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Data Mining Tools
• Queries can be executed to find any injection, or group of injections.
• Search fields include any sample criteria or any calculated result!
Example:
• Find all Standards
• Injected on HPLC_System_13
• During the previous 6 months
• Using Instrument Method TM122
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• Using Instrument Method TM122
• With a peak name containing Methyl
Data Mining Tools
• Example: Samples injected over a 3 year period for a stability study
• Samples can be located, in this case Lot Number is used
• Once the data is located it can be compared as if it belongs to the same
sequence:
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Data Mining Tools
• Using Excel-like functionality the report table can immediately indicate a Pass or Fail result:
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Data Mining Tools
• The data can also be viewed graphically in charts:
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G367
G367 6 Mo
G367 12 Mo
G367 18 Mo
G367 24 Mo
G367 36 Mo
Here the active
ingredient is shown
degrading over time
Data Mining Tools
• The data can also be viewed graphically in charts:Rel.Area (Imp_1)
And the Relative Area
of the impurity over the
same time period
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G367
G367 6 Mo
G367 12 Mo
G367 18 Mo
G367 24 Mo
G367 36 Mo
Example: Column Performance
• Charting column performance throughout a sequence, or multiple sequences over a period of time:
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Spreadsheet Based Reporting
• The Excel-like report environment is both familiar and intuitive
• If Excel is used for additional calculations they can be included in Chromeleon CDS
• No data transcription or
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• No data transcription or export to Excel is required
• Results are generated instantly
Spreadsheet Based Reporting
Excel-like formulae can be included for custom
calculations
In this case the operator has configured a report to flag
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configured a report to flag out of specification results.
Report Designer - Functions
Many Excel-like functions available – use identical syntax
AVERAGE
IF / AND / OR
STDEV
COUNT
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COUNT
SUM
LOOKUP
etc……
Intelligent Run Control
• Chromeleon 7.2 CDS automates injection decisions, based on previous results and chromatographic specifications
• Improves analytical efficiency
• Improve or standardise product quality
• Reduces solvent & analyte use
• Reduces results review process
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Intelligent Run Control
Examples:
• Component Carry Over
• Test : Is area of Peak A < 5 mAU/min for a Blank Injection?
• Pass Action: continue
• Fail Action: add Blank injection to sequence, and inject
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• Automatically Dilute a Sample (AutoDilution)
• Test: Is a component concentration within the tolerated range?
• Pass Action: continue
• Fail Action: dilute the sample, insert an injection into the
sample list and re-inject.
Intelligent Run Control
• Example of an Unconditional Action
• Set the desired action and then set properties for that action
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Fraction Collection
• Chromatogram Plot - capable of showing tube stripes
• Rack View – shows the collected fractions and the fraction details
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• Wizard/Editor for Fraction Collection
• Straightforward setup of fraction collection parameters
• Preview of fractionation using an existing chromatogram
Fraction Collection
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MS is fully integrated within Chromeleon 7.2 CDS
• Chromeleon 7.2 CDS supports MS instrument control and data processing in an enterprise environment.
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Chromeleon Goes MS
• Enables existing Chromeleon CDS functionality for GC-MS and LC-MS
• Client/server, central data storage + 21 CFR Part 11/GLP/GMP compliance
• Extends Data Processing to support typical MS quantitation workflows
• Multiple dynamically linked Data Views and Component Traces
• Support for multi-channel MS quantitation
• Library searches, versioning and charting
• Industry leading Reporting tools
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• Industry leading Reporting tools
• Manage entire laboratory centrally from 1 CDS: LC/IC/GC/MS
Instrument Control
• Chromeleon 7 CDS can control Thermo Scientific LC, GC, IC and MS.
• Chromeleon 7 CDS can control 3rd Party LCs and GCs
• Agilent 1100, 1200, 1260, 1290, G1888, 6850,
6890, 7890
• Waters Acquity, Alliance
• Shimadzu LC10, LC2010, LC20, LC30,
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• Shimadzu LC10, LC2010, LC20, LC30,
GC2010/2014
• Perkin Elmer 200, Clarus, Autosystem XL
• CTC PAL
• Gilson and Varian LC
• And many more.....
Chromeleon CDS Installation
• Can consist of a single computer used for controlling one or two instruments and data processing
• Instruments are Thermo Scientific LC, GC or IC, Third Party GC or Third
Party LC
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• Or of a network…
• … with multiple computers and instruments …
• … clients to process data or remotely control instruments …
• … and a central location to directly store all chromatography data
Chromeleon Domain
Chromeleon CDS Installation
Chromeleon Domain
Controller
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Network
Central
Server
Chromeleon 7 CDS – Simply Intelligent
• Contains the Intelligent Functionality needed to generate the result!
• Uses Operational Simplicity to minimize the steps and time needed to generate the result!
• Offers state-of-the-art technology:
• Modern User Interface
• Peak detection and integration tools
• eWorkflows – THE framework for Operational Simplicity
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• Advanced Interactive Charting
• Advanced Reporting
• Intelligent Run Control
• Control of GC, IC, LC, MS and Third Party Instrumentation
• Full Network Scalability