an introduction to lipidomic workflows
TRANSCRIPT
An Introduction To Lipidomic Workflows
John Bowden (University of Florida)Gavin E. Reid (University of Melbourne)Valentina Pirro (Eli Lilly and Company)
Course Outline
Sample Preparation Strategies
Mass Spec Analysis Workflow
Data Handling
Q&A
External Resources
Structural Identification
Introductory Level
Goal is to provide information andresources on current workflows, from theexperimental design stage to datadissemination, so that attendees can learnhow to adequately design, perform, andanalyze data from lipidomics experiments.
Basic knowledge of analytical chemistryand mass spectrometry is required
Overview of Lipid Biology
Imaging and datamatrix composition
Y
X
m/z
m/z 281
m/z 834m/z 788
LC Drift Time Ion Mobility MS*
*doi: 10.1021/acs.analchem.8b01527
*doi:10.1073/pnas.94.6.2339; doi:10.1039/C6AN00015K
*doi:10.1073/pnas.94.6.2339; doi:10.1039/C6AN00015K