an overview of ivf/icsi
TRANSCRIPT
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An overview of IVF/ICSI
DR.RENUKADEVI SENIOR EMBRYOLOGIST
ARC International Fertility & Research Centre
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INFERTILITY?
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OVERVIEW OF INFERTILITY
• Definition: 1 year of well-timed, unprotected intercourse without a pregnancy
• 10-15% of population is infertile
• 15-20 % of couples have unexplained infertility (work-up is negative)
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CAUSES OF INFERTILITY
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Assisted Reproductive Techniques (ART)
Any treatment that deals with “means of conception other than vaginal intercourse” is termed as ART. NICE guideline 2013
IUI – Intra Uterine Insemination (Husband / Donor)
IVF + ET – In Vitro Fertilization + Embryo transfer
ICSI – Intra Cytoplasmic Sperm Injection
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IUI • Injection of washed prepared sperms into the uterine cavity through a fine catheter during pre-ovulatory phase in a natural or stimulated cycle.
• Although pregnancy may not occur as quickly, a policy of initial treatment by IUI will probably save 20% of couples from moving onto IVF
• After 3-4 cycles of failed IUI treatment, patients should be encouraged to opt for IVF
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Indications for Intra Uterine Insemination (IUI)
- At least one Fallopian tube must be normal and patent - Mild male infertility - Unexplained infertility - Ovulatory dysfunction, PCOS - Mild endometriosis - Cervical factors - Coital problems - Immunological factors - HIV, HBs Ag infection - Donor Sperm
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SPERM PREPARATION
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Intrauterine Insemination (IUI)
Goal is to Maximize the Chance of Fertilization• Increase Number of Eggs• Position Sperm Closer to Eggs
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I.V.F.
In VitroFertilization
“Test tube babies”
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Indication for IVFI. IVF as first line infertility treatment
- Tubal pathology (severe, non-repairable)- Donor Oocyte- Genetic Surrogacy- PGD (Possibility of genetically transmitted disease) - Fertility preservation in cancer patients- Where ICSI is indicated (Azoospermia)
II. IVF following failed cycles of IUI- Usually up to six cycles of IUI with controlled ovarian
stimulation are recommended, but there are situations where couples should move to IVF earlier.
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IVF and ET• In Vitro Fertilization (IVF) and Embryo Transfer (ET) are the basic ART
for all related technology. These include:
- Intra Cytoplasmic Sperm Injection (ICSI)
- Assisted hatching
- Pre-implantation Genetic Diagnosis (PGD)
- Cryopreservation
- Donor oocyte IVF programs
- Donor embryo (genetic surrogacy)
- Intracytoplasmic Morphologically selected
Sperm Injection (IMSI)
- And many more
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Various steps of an IVF treatment cycle
Pre IVF work-up Ovarian stimulation Monitoring
Preparation of sperms Oocyte retrieval
Embryo transfer Luteal Support
Ovulation induction
In Vitro Fertilization
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SPERM PREPARATION
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CONVENTIONAL -I.V.F.
• Simply put IVF is adding a man's sperm to his female partners eggs in the laboratory to produce embryos. In vitro fertilization is an option for many couples who cannot conceive through conventional therapies. These embryos are put back into the female partner's uterus (womb) after 3 to 5 days of being in the incubator.
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CONVENTIONAL -I.V.F.
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IN VITRO EMBRYO DEVELOPMENT
COC at the time of retrieval
M II oocyte with a PB (Mature)
2 PN embryo
4 cell embryo 8 cell embryo Fully grown blastocyst
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Intra cytoplasmic Sperm Injection (ICSI)
• Unprecedented successful development of ART which has revolutionized the management of severe male infertility.
• The procedure involves the direct injection of a single sperm into the egg cytoplasm
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INDICATIONS FOR ICSI• Sperm completely absent from the ejaculate
(azoospermia)• Sperm present in low concentrations (oligospermia).• Poor sperm motility (asthenospermia)• Poor sperm morphology (teratospermia)• Sperm retrieved by surgical techniques (for example
TESA, TESE)• If pre-implantation genetic diagnosis (PGD) is being used
to screen embryos for a specific genetic disorder• History of fertilization failure in conventional IVF
attempts
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ICSI
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IN VITRO EMBRYO DEVELOPMENT
COC at the time of retrieval
M II oocyte with a PB (Mature)
2 PN embryo
4 cell embryo 8 cell embryo Fully grown blastocyst
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EMBRYO TRANSFER
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HOW MANY EMBRYOS ARE TRANSFERRED?
• Related to age and embryo quality– <35 = 2– 35-37 = 2-3– 38-40 = 3-4– >40 = up to 5
– For patients with 2 or more failed IVF cycles, or a poor prognosis, can add more based on clinical judgment
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EMBRYO TRANSFER
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EMBRYONIC DEVELOPMENT
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