analytical nmr
TRANSCRIPT
Nuclear magnetic resonance
spectroscopy
• Def. :-known as NMR spectroscopy, is a research technique that exploits the magnetic properties of certain a certain atomic nuclei.
• . It determines the physical and chemical properties of atoms in which they are contained.
•Basic NMR techniques:-
1-The NMR sample is prepared in a thin-walled glass tube - a NMR tube.
2-When placed in a magnetic field, NMR active nuclei (such as 1H or 13C) absorb electromagnetic radiation .
• Now we have to answer an important question, on which phenomenon it relies ?
• As an answer for this question we will ay it depends on nuclear magnetic resonance.
• Importance :-1-Can provide detailed information about the
structure, dynamics, reaction state, and chemical environment of molecules.
2-is used by chemists and biochemists to investigate the properties of organic molecules at a frequency characteristic of the isotope.
•Now it is time to talk about the chemical shift.
• What is chemical shift?
•Chemical shift:-is the resonant frequency of a nucleus relative to a standard, this standard is often tetramethylsilane, Si(CH3)4, abbreviated TMS.
Example of the chemical shift:• NMR spectrum of hexaborane
B6H10 showing peaks shifted in frequency, which give clues as to the molecular structure.
• Importance :-The chemical shift provides information about the structure of the molecule.
• Do you remember spin-spin coupling?
• It a special type of it. This coupling arises
from the interaction of different spin states
through the chemical bonds of a molecule
and results in the splitting of NMR signals.
• Importance:-it provides detailed insight
into the connectivity of atoms in a
molecule.
NMR analysis applications
1-Chemical structure analysis of liquids and dissolved solids
2-Deformulation of products
3-Quantification of mixture components
NMR analysis capabilities :
1- Quantitative NMR Analysis (qNMR)
solid State Nuclear Magnetic Resonance-23-Solution State NMR
4-NMR Chemical Composition
5-Elucidation
NMR analysis expertise.
laboratories provide clients analytical data regarding the type, quantity and arrangement of atoms in chemical systems, liquids and solids.
1-Quantitative NMR Analysis :-
Intertie NMR laboratories provide qNMR (Quantitative Nuclear Magnetic Resonance Spectroscopy) analysis applications such as quality control, drug impurity analysis, and metabonomicsin agrochemicals, chemicals, plastics processing and the pharmaceutical industry
2-Solid State Nuclear Magnetic Resonance
Solid state Nuclear Magnetic Resonance (NMR) is a specialized characterization technique used to determine chemical composition or structural properties in a variety of industrially relevant materials. Solid State NMR brings the benefits of solutions NMR (detailed chemical structural information without the need for standards
Heterogeneous catalysts used for chemical manufacture, petroleum processes, purification, and air separationDetermine framework composition of zeolites and changes with activation or useIdentify adsorbed species in porous materialsCharacterize key functionalities in insoluble residues
Determination of catalyst
• Correlation spectroscopy:-
•is one of several types of two-dimensional nuclear magnetic resonance (NMR) spectroscopy .This type of NMR experiment is best known by its acronym .
• Biomolecular NMR spectroscopy:-• A-proteins:-Much of the innovation within
NMR spectroscopy has been within the field of protein NMR spectroscopy, an important technique in structural biology .
• B-Nuculeic acids :-
• is the use of NMR spectroscopy to obtain information about the structure and dynamics of polynuculeic acid, such as DNA or RNA.
• Note :-Nucleic acid and protein NMR spectroscopy are similar but differences exist. Nucleic acids have a smaller percentage of hydrogen atoms, which are the atoms usually observed in NMR spectroscopy.
1937 Rabi predicts and observes nuclear magnetic
resonance
1946 Bloch, Purcell first nuclear magnetic resonance of
bulk sample
1966 Ernst, Anderson Fourier transform NMR
1944 Physics Rabi (Columbia)
1952 Physics Bloch (Stanford), Purcell (Harvard)
1991 Chemistry Ernst (ETH)
2003 Medicine Lauterbur (University of Illinois in Urbana)
NMR History
Some NMR Web Sites
The Basics of NMR by J.P. Hornak Hypertext based NMR course http://www.cis.rit.edu/htbooks/nmr/nmr-main.htm
Integrated Spectral Data Base System for Organic Compoundshttp://www.aist.go.jp/RIODB/SDBS/menu-e.html
Educational NMR Software All kinds of NMR softwarehttp://www.york.ac.uk/depts/chem/services/nmr/edusoft.html
NMR Knowledge Base A lot of useful NMR linkshttp://www.spectroscopynow.com/
NMR Information Server News, Links, Conferences, Jobshttp://www.spincore.com/nmrinfo/
Technical Tidbits Useful source for the art of shimminghttp://www.acornnmr.com/nmr_topics.htm
BMRB (BioMagResBank) Database of NMR resonance assignmentshttp://www.bmrb.wisc.edu/
DutiesNumbersNames
Data Collector20452Sally Yousry
Data Collector + summarizer + powerpoint
+ leader
20453Sameh Gamal
presenter20455Sandra Maher
Data Collector20456Sandy Maged
Data Collector20457Sahar Ezzat
Data Collector20458Sahar Mahmoud
Data Collector + powerpoint + presenter20459Salma Ahmed
Data Collector + summarizer20460Salma Gamal