ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS Volume 12

Animal Cell Technology: Basic & Applied Aspects

Volume 12

Proceedings of the Thirteenth Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT),

Fukuoka-Karatsu, November 16-21, 2000

edited by

SANETAKA SHIRAHATA

KIICHIRO TERUYA

and

YOSHINORI KATAKURA Department of Genetic Resources Technology,

Faculty of Agriculture, Kyushu University, Fukuoka, Japan

SPRINGER-SCIENCE+BUSINESS MEDIA, B.V.

A C.I.P. Catalogue record for this book is available from the Library of Congress.

ISBN 978-90-481-5934-5 ISBN 978-94-017-0728-2 (eBook) DOI 10.1007/978-94-017-0728-2

Printed on acid-free paper

Ali Rights Reserved © 2002 Springer Science+Business Media Dordrecht

Originally published by Kluwer Academic Publishers in 2002 No part of the material protected by this copyright notice may be reproduced or

utilized in any form or by any means, electronic or mechanical, including photocopying, recording or by any information storage and

retrieval system, without written permission from the copyright owner.

Contents

Preface

Symposium

Approaches to Human Gene Therapy for Next Century

Improvement of adenovirus vectors for gene transfer H. Mizuguchi, T. Hayakawa

Gene therapy for genetic disease and cancer R. S. Mcivor

Application of Chip Technology in Molecular and Cellular Biology

Gene expression profiling of mouse postnatal cerebellar development Adaptor-tagged competitive PCR and its application to the mammalian nervous system

K. Katoh

Mesenchymal Stem Cells for Reconstructive Surgery

Generation of regenerated cardiomyocytes and its therapeutic use for cell transplantation into failing heart

K. Fukuda

Advanced Functional Foods and Water for Prevention of Diseases

Reduced water for prevention of diseases S. Shirahata

Clinical applications of electrolyzed-reduced water H. Hayashi, M. Kawamura

Molecular Biology of Fish Cells in vitro

Identification and characterization offibronectin isoforms from zebrafish Q. Zhao, X. Liu, P. Collodi

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7

13

19

25

31

37

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Neurogenesis in zebrafish embryo cell cultures N. N. Singh, D. Barnes

Search for Bioactive Substances Using Animal Cells (Satellite Symposium Karatsu)

Assay systems for screening food factors that have anti-proliferative and anti­invasive potential using rat ascites hepatoma cells in culture

43

Y. Miura, K. Yagasaki 51

Search for chemopreventive agents on the basis of the involvement of oxidative stress and inflammation in carcinogenesis

A. Murakami, Y. Nakamura, K. Koshimizu, H. Ohigashi 57

Functional analysis ofsphingolipids using a specific enzyme and animal cells M~ ~

Session

Cell Culture Engineering

Cell cultures and reverse transcriptase activity from a tumor of a moray eel C. Buck, C. Walsh, R. Davis, A. Toumadje, K. Kusumoto, A. Helmrich, C. Chapline, P. Mericko, D. Barnes 67

A simplified general artificial neural network for the modelization of culture kinetics of different CHO strains

T. Marique, M. Cherlet, V. Hendrick, F. Godia, G. Kretzmer, J. Werenne 73

Cell growth on modified polystyrene surface/ adhesion and detachment properties

V. Hendrick, E. Muniz, G. Geuskens, J. Werenne 77

Whole cell-smart microsensor: a first approach towards interfacing live cells and electronics

0. Charlier, B. Mommens, M. J. Goffaux, T. Marique, V. Hendrick, L. De Vos, J. W erenne 81

Copolymer of amino-acid and urethane as an artificial extracellular matrix to enhance the primary porcine hepatocytes attachment, proliferation and function

Q. Zhang, C. Wang, Y. Shiba, T. Ohyama, S. Uchida, M. Kodama 85

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Primary culture of rat hepatocytes on an oxygen permeable mambrane H. Kurosawa, R. Yasuda, Y. Amano 9I

Hydroxyapatite-pulp composite fiber sheet bed: a new material for the continuous culture ofCHO-KI cells

H. Kawakatsu, S. Ide, K. Koga, Y. Okuda, S. Shirahata 97

Relationship between cell cycle phases and monoclonal antibody production in microcarrier perfusion culture of recombinant CHO cells

Y. Makimoto, E. Takahashi, H. Takasugi I 03

Influence of bcl-2 over-expression on cell growth and antibody productivity ofNSO cells in perfusion and chemostat cultures

B. T. Tey, R. P. Singh, M. AI-Rubeai I09

Dialysis perfusion culture of animal cells for the production ofbiologicals H. Yam~i, B. Amos, A. N. Emery, M. AI-Rubeai 115

Characterization of growth properties through morphological observation in culture of anchorage-dependent cells

M. Kino-oka, H. Hirai, R. Umegaki, M. Taya I2I

Application ofhepatocytes isolated from slaughtered pig for bioartificialliver support system

T. Ohyama, T. Mukaiyama, Y. Shiba, C. Wang, Q. Zhang, M. Kodama, 1. Kano, S. Enosawa, T. Tokiwa I 27

Culture ofhepatocytes using non-woven PTFE fabric coated with copolymer ofpoly(amino acid) urethane

Y. Shiba, T. Mukaiyama, C. Wang, T. Ohyama, Q. Zhang, M. Kodama, S. Enosawa, T. Matsumura I 33

Production of Biologicals

Hypoxia-induced production of recombinant erythropoietin using hypoxia­response enhancer

S. Masuda, S.-K. Moon, T. Kambe, M. Nagao, R. Sasaki I39

Direct control of cell metabolism by physicochemical conditions to increase productivity of glycosylated proteins

V. Hendrick, P. Winnepenninckx, C. Abdelkafi, 0. Vandeputte, T. Marique, G. Renemann, A. Loa, G. Kretzmer, J. Werenne 143

Large scale cGMP manufacturing ofpDNA for gene therapy R. Werner, R. Necina, K. Meyer 151

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A novel application of granulation technology to improve physical properties and biological performance of powdered serum-free culture media

D. Jayme, R. Fike, R. Radominski, B. Dadey, R. Hassett, D. Cady 155

High level production and characterization of recombinant human Thrombopoietin in CHO cells

H.-K. Ahn, S.-K. Park, S.-W. Lim, S.-K. Park, Y.-W. Koh 161

Characterization of recombinant transglutaminase 1 and 3 expressed in baculovirus system

K. Hitomi, K. lkura, M. Maki 167

Construction of improved mammalian expression vectors N. Nakamichi, T. Miyata, Y. Hua, T. Matsumura 173

Immunoglobulin production stimulating activity of heat-denatured lysozyme T. Sugahara, Y. Yamada, Y. Ueda, S. Matsubara, T. Sasaki 179

Enhancement of monoclonal antibody production ofhuman hybridomas by retinoids

Y. Inoue, M. Hatazoe, S. Shirahata 185

CB.Hep-1 hybridoma growth and antibody production using protein-free medium in a hollow fiber bioreactor

R. Valdes, N. Ibarra, M. Gonzalez, T. Alvarez, J. Garcia, R. Lambias, C. A. Perez, 0. Quintero, R. Fischer 189

Production of Mab CB.HEP-1 for the purification of rHBsAg at high scale R. Valdes, B. Reyes, N. Ibarra, R. Hernandez, M. Gonzalez, S. Padilla, A. Tamayo, M. Aleman, J. A. Montero, T. Alvarez, A. Figueroa, L. Dorta, J. Garcia, D. Fernandez, C. Garcia, 0. Herrera, H. Gomez, G. Calas, Y. Gonzalez, L. Gomez, A. Agraz, J. L. Vega, L. Herrera 195

Functional Cell Lines

Establishment of novel cell line for bioartificialliver S. Terada, N. Yamamoto, A. Ogawa, T. Kumagai, M. Miki, T. Fujita, E. Suzuki 201

Glycoengineering

Improvement ofsialylation in chinese hamster ovary (CHO) cell culture by feeding N-acetylmannosamine

Y. Oba, T. Fletcher, M. Hisaka, W. J. Treat 207

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Immunologicals, Monoclonal Antibodies, and Vaccines

The role dendritic cells play in the cytokine responses in Peyer's patches A. Sato, M. Hashiguchi, S. Hachimura, S. Kaminogawa 213

Characterization of cancer specific human monoclonal IgM antibodies produced ty hybridomas derived from a patient with adult T-cell leukemia

T. Kawahara, A. Ichikawa, Y. Katakura, K. Teruya, T. Yoshida, M. Kikuchi, S. Hashizume, S. Shirahata 219

Production of genetically engineered antigen specific human monoclonal lgE antibody

S.-Y. Shim, Y. Katakura, K. Teruya, A. Ichikawa, S. Shirahata 225

CD8+ T cells negatively regulate the antibody production from the human PBMC being immunized in vitro with antigen

M. Yamashita, Y. Katakura, A. Ichikawa, K. Teruya, S. Shirahata 231

Transplantation, Artificial Organs, and Organ Substitutes

Effect ofbiomaterials and nutrient factors on chondrogenesis of human chondrocytes

M. S. Rahman, T. Tsuchiya 235

Polarity and bile canaliculi-like channels in self-assembled rat hepatocyte spheroids

S. F. Abu-Absi, J. R. Friend, L. K. Hansen, W.-S. Hu 241

A tissue inducible small diameter vascular graft coated with PAU (copolymer of amino-acid and urethane)

C. Wang, Q. Zhang, S. Uchida, M. Kodama 247

Studies on the biocompatibility of artificial organs and tissue engineered products: Embryonic neuronal cell differentiation on the various kinds of biodegradable polymeres

T. Tsuchiya, Y. ltahashi, T. Ichikawa, A. Ichikawa 253

Analysis of immunosuppressive factors expressed in serum ofliver transplanted rats

T. Nakano, S. Kawamoto, T. Sasaki, K. Maekawa, T. Aki, S. Shigeta, 0. Suzuki, S. Goto, K. Ono 257

X

Expression and amplification of glutamine synthetase gene for constructing ammonia-metabolizing cell lines in hybrid bioartificialliver support system

T. Omasa, K. Kim, S. Hiramatsu, Y. Katakura, M. Kishimoto, T. Matsumura, S. Enosawa, S. Suzuki, H. Amemiya, K. Suga 263

Reversion of transformed phenotype ofpolyetherurethane-induced tumor cells by Cx43 transfection

A. Ichikawa, T. Tsuchiya 269

Transgenic Animals

Expression of exogenous genes at the germinal ridge of germ line chimeric chickens

H. Furuta, N. Fujihara 275

Possible transfer of intra-testicularly injected foreign DNA to generations in chicken

T. Arima, N. Fujihara 281

Production of transgenic birds using pantropic retroviral vectors S. Mizuarai, K. Ono, K. Kanbayashi, K. Nishijima, M. Kamihira, S. Iijima 287

Use of cultured primordial germ cells for production of transgenic fish C. F. Ma, L. Fan, R. Ganassin, N. Bois, P. Collodi 293

Safety and Regulation

Analysis of antigenicity of collagenase-treated gelatin with antibodies secreted by immortalized human B cells

H. Shinmoto, K. Yamagishi, T. Kimura, M. Suzuki, Y. Sakai

Cell Regulatory Factors and Signal Transduction A chromatin remodeling factor, BRM has important roles in cell differentiation

Y. Machida, Y. Inayoshi, K. Murai, K. Miyake, S. lijima

Human amniotic epithelial (HAE) cells produce erythropoietin A. Ogawa, S. Terada, M. Miki, K. Matsuura, A. Hoshika, N. Sakuragawa

299

303

309

Antiproliferative and growth-stimulating activities of synthetic cytokinin analogs

F. Franek, M. Strnad, L. Havlfcek, V. Siglerova

Modulation of cell proliferation by the over-expression of the c-myc gene V. Ifandi, M. AJ-Rubeai

Pleiotrophic functions of erythropoietin and tissue-specific regulation of its expression

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315

321

M. Nagao, S. Masuda, M. Chikuma-Esaki, T. Kobayashi, R. Sasaki 325

Characteristics of orally tolerized CD4 T cells K. Asai, S. Hachimura, T. Toraya, S. Kaminogawa 331

Lysozyme-dependent proliferation switch: Hematopoietic and hybridoma cell growth control with the use ofantibody/gpl30 chimera

M. Kawahara, A. Natsume, S. Terada, K. Kato, K. Tsumoto, I. Kumagai, M. Miki, W. C. Mahoney, H. Veda, T. Nagamune 337

Human fibroblast growth factor binding protein HBp17 enhances the tumorigenic potential of immortalized squamous epithelial cells

X. Liu, S. Shi, J.-H. Chen, D. Wu, M. Kan, Y. Myoken, T. Okamoto, J. D. Sato 343

The protective anti-oxidant effects ofKefir on SFME neural stem cells K. Kusumoto, A. Helmrich, P. Mericko, L. Chen, J. D. Sato, S. Shirahata, S. Tokumaru, D. Barnes 353

The effect of high electromagnetic fields on the growth characteristics of human cell lines

W.-T. Chung, S.-H. Lee, M.-K. Lee, J.-H. Lee, Y.-S. Park, S.-Y. Lee, H.-Y. Lee 359

Downregulation of high affinity IgE receptor FcERI expression in the human basophilic KU812 cells by a tea catechin

Y. Fujimura, H. Tachibana, K. Yamada 365

Putative protection mechanism ofCTL from killing by their own perforin N. Eto, S. Kurokui, S. Ikeda, K. Sone, A. Hirashima, Y. Fujimura, K. Tanino, H. Tachibana, M. Yasuda, C-C. Liu 371

Identification of differentially expressed genes in atopic dermatitis-prone NC/Nga mouse

I. Yamaguchi, S. Kawamoto, T. Aki, S. Shigeta, 0. Suzuki, K. Ono 379

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Oxidative stress affects telomere maintenance in cancer cells K. Yoshizaki, Y. Katakura, S. Shirahata

Functional Substances in Food and Natural Sources

Suppression of hepatoma cell invasion in culture by resveratrol and its modes of action

385

Y. Kozuki, Y. Miura, K. Yagasaki 389

Mechanisms of invasion inhibition ofhepatoma cells by tea components and sera from tea-fed rats

G. Zhang, Y. Miura, K. Yagasaki 395

A protein derived from Flammulina velutipes that enhances paracellular permeability ofCaco-2 intestinal epithelial cells

A. Narai, H. Watanabe, T. Tomita, M. Shimizu 401

Long term neurite outgrowth enhancing effect and neurite regeneration effect of an active substance from a brown alga, Sargassum macrocarpum on rat pheochromocytoma PC12D cells

C. K. Tsang, Y. Kamei 407

Anti-allergic catechins of tea (Camellia Sinensis) M. Maeda-Yamamoto, K. Kawamoto, N. Matsuda, M. Sano, N. Suzuki, M. Yoshimura, H. Tachibana, Y. Kawakami, T. Kawakami, K. Hakamata 415

Bioavailabilities and inhibitory actions of trigonelline, chlorogenic acid and related compounds against hepatoma cell invasion in culture and their modes of actions

K. Yagasaki, R. Okauchi, Y. Miura 421

Changes in the relevant test parameters of 101 diabetes patients under the influence of the so-called "Nordenau-Phenomenon"

Z. Gadek, S. Shirahata 427

Effect of food component on differentiation and maturation ofpre-adipocyte M. lio, A. Tajima, Y. Kihara, M. Sakono, N. Fukuda 433

Inhibitory effect of a chinese plant Saussurea inrolucrata on hyaluronidase and on ~-hexosaminidase release from rat basophilic leukemia (RBL-2H3) cells

H. !soda, S. Ru, H. Seki, T. Maekawa 439

Apoptosis and receptor-mediated signal regulation of cultured tumors by glycoprotein from Meretrix lusoria

xiii

Z. Kong, C. Hwang, C. Lai 443

Immue bioactive substances from fish larvae "Buii-Ary", Engraulis japonicus Z.Kong,S.Gau,K.Feng,TChen 449

Disease-preventing activities of plant products H. Nagashima, K. Nakamura, T. Goto 455

Gamma-linolenic acid suppresses lgE production in vitro as well as in vivo S. Kawamoto, M. Hamada, M. Kita, T. Aki, S. Shigeta, 0. Suzuki, K. Ono 461

Regulation of serum leptin level by dietary conjugated linoleic acid in Sprague-Dawley rats

M. Yamasaki, K. Mansho, Y. Ogino, M. Kasai, H. Tachibana, K. Yamada 467

Apoptosis induction and cell cycle arrest in T-lymphoblastic leukemic cells (CEMSS) by damnacanthal

A.M. Ali, I. Norhadiani, L. S. Yazan, S.M. Mohammad, N.H. Lajis, R. A. Rahim, J. S. Dhaliwal, N. Abdullah, S. H. Inayat-Hussain 473

Animal Cells for in vitro Assay

An in vitro approach to evaluation of the cross talk between intestinal epithelial cells and macrophages

M. Manabe, H. Kanzato, M. Shimizu 479

Alkylphenolic compounds and their effect on the injury rate, survival and acetylcholinesterase activity of the rat reuronal cell line PC12

T. P. N. Talorete, H. !soda, T. Maekawa 485

Author Index 491

Subject Index 497

Preface

The thirteenth annual meeting of Japanese Association for Animal Cell Technology (JAACT2000) "ANIMAL CELL TECHNOLOGY FOR CREATION OF NEW ERA" was held in Fukuoka, November 17-21, 2000. The satellite symposium Karatsu "SEARCHES FOR BIOACTIVE SUBSTANCES USING ANIMAL CELLS" was also held in Karatsu, November 16, 2000. Followed by JAACT'98 Kyoto, this meeting focused on important and exciting recent developments in the field of animal cell technology from basic and applied aspects. We welcomed more than 340 participants including 60 foreign participants from 15 countries and accepted 161 presentations (Plenary lecture, 4; educational lecture, 1; presentations by symposists, 38; workshops, 2; oral presentations, 42; poster presentations, 74).

The fields of 9 symposia were "Neural stem cell and brain regeneration", "Approaches to human gene therapy for next century", "Mesenchymal stem cells for reconstructive surgery'', "Advanced functional foods and water for prevention of diseases", "Application of chip technology in molecular and cellular biology", "Application of recombinant baculovirus technology in the biopharmaceutical industry", "Molecular biology of fish cells in vitro", and "Searches of bioactive substances using animal cells".

All presentations (oral and poster) have been brought together to the following themes.

1. Cell culture engineering 2. Production of biologicals 3. Functional cell lines 4. Glycoenginering 5. Immunologicals, monoclonal antibodies, and vaccines 6. Transplantation, artificial organ, and organ substitutes 7. Transgenic animals 8. Safety and regulation 9. Cell regulatory factors and signal transduction 10. Functional substances in food and natural sources 11. Animal cells for in vitro assay

This volume of Proceedings contains the reflection of this vast amount of new information. It is no doubt that animal cell technology plays far more important role in the development of the quality of life in the 21 81 century and therefore this volume should greatly contribute to find the important, urgent and rewarding targets to be challenged.

The editors express their sincere thanks to all participants at the Meeting, to the organizers of the Symposium sessions, to the members of the organizing committee and program committee and the staffs and students of Saga University and Kyushu University for their dedication in assuring the success of the Meeting.

The Editors

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