ANNUAL REPORT - DEPARTMENT OF PHYSIOLOGYUNIVERSIT Y OF KANSAS MEDICAL CENTER

July 1, 1963 - June 30, 1964

ACADEMIC STAFF

E. B. Brown, Jr., Ph. D. ProfessorAlan M. Thompson, Ph. D., Associate ProfessorNorman Loofbourrow, Ph. D., Associate ProfessorLawrence Sullivan, Ph. D., Assistant ProfessorDonald Johnson, Ph. D., Assistant Professor (Joint appoint-

ment in Obstetrics and Gynecology)Kenneth Goetz, Ph. D., InstructorJohn K. Leach, 1\1.D., LecturerC. Frederick Kittle,M. D., Visiting Associate Professor,

Department of Surgery

POSTDOCTORAL FELLOWSVirginia Tucker, M. D., Department of PediatricsJames Evans, M. D., Division of AnesthesiologyBenjamin Razon, M. D., AID Fellow, Far Eastern University,

Manila, PhilippinesHiroshi Aoki, M. D., Surgical Fellow from Japan.

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GRADUATE STUDENTS

Richard L. ClancyRobert W. Hamilton, Jr.Robert Thurber

Dale MayhewChester Crawf.ordLilly KungMomoko YasudaDexte r Bellis

Gary SlickFranklin Richard Darrow

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TEACHING

The medical school course for freshmen was reorganized so thatNeurophysiology and Neuroanatomy were separated. This meant that thephysiology course now consisted of Medical Physiology #270, 8 quartercredits and Physiology #271, 7 quarter credits. These courses wereagain offered during the fall and winter quarters, respectively. TheHistology course was removed from the fall quarter and put into thespring quarter. The freshman year, then, consisted of Physiology andBiochemistry during the fall quarter, Physiology and Biochemistryduring the winter quarter, until the last two weeks at which time theintegrated course in Endocrinology was again presented, and Anatomyduring the spring quarter.

Annual Report - Dept. Physiol.KUMC, July 1, 1963-June 30, '64.Page 2

During the year, three graduate courses and two graduate seminarswere presented. Dr. Thompson's course, Physiology 310, Transportand Permeability (2 credits) was offered during the winter quarter; Dr.Sullivan's course, Physiology #315, Renal Electrolyte Excretion (2 credits);and Dr. Loofbourrow's course Physiology #322, Physiology of the NervousSystem (3 credits) were offered during the spring quarter of 1964. Dr.Thompson gave a seminar on Saturday mornings on Cerebral Circulationin fall of 1963. During the spring quarter Drs. Brown and Kittle directeda Graduate Seminar (Physiology #35l) on Pulmonary Mechanics. All fiveof these offerings were r[lade available as electives for medical studentsand medical center staff.

In addition to the Senior Staff members, Postdoctoral Fellows Drs.Tucker, Evans, and Razon; and Graduate Students Clancy, Hamilton, andThurber each carried full responsibility for one group of students in themedical school physiology laboratory course.

A Surgery-Physiology Conference was held every Wednesday duringthe academic year for students and staff.

During the summer of 1963 the integrated course in Basic HumanBiology for Nurses was taught for the first time on this campus. Thecourse followed essentially the same format which had been used inprevious years at Lawrence, Kansas. The Physiology portion of thecourse was taught by Drs. Brown and Loofbourrow.

Current course offerings of the department are described as follows:COURSE DESCRIPTIONS (Credits are quarter hours.)

270.

271.

279.

Medical Physiology (8 credits). A systematic coverage in lecture,laboratory, and conferences of the following topics: Transportmechanisms, membrane phenomena, cardiovascular system,respiratory system, blood, acid-base balance. Staff.

Medical Physiology (7 credits) Continuation of 270 to cover gastro-intestinal system, renal system, temperature regulation, fluid andelectrolyte metabolism; nervous system. Staff.

Endocrinology (3 credits). This course is designed to present thechemistry and physiology of the steroid, protein and non-proteinhormones, as well as the embryology and morphology of the endocrineglands. Lectures devoted to biosynthesis, storage, release, transportmechanisms of action and degradation of hormones. Discussion andlaboratory periods devoted to study of currently used techniques ofin ve stig ation. Staff.

286.

287.

310.

315.

320.

322.

Annual Report - Dept. Physio!.KUMC, July 1, 1963 - June 30, 164Page 3

282.Advanced Topics (1-7). Special studies designed and arranged onan individual basis to allow a student to pursue a particular sub-ject through reading, special laboratory work, and conferenceswith a senior staff member. Staff.

Reproductive Physiology (5). Selected topics in the anatomy andphysiology of the male and female reproductive systems. Read-ings, seminar presentations, movies, laboratory demonstrations.Greenwald.

Readings in Reproductive Physiology (4). The student will select atopic of interest and thoroughly review the literature. At bi-monthly intervals he will report his findings to the instructor.A term paper will summarize his readings. Greenwald.

Transport and Permeability (2 credits). Selected topics illustratingcurrent concepts of material transport and membrane permeability.Primarily lecture, but written and oral reports will be required.Content will vary from year to year, but may include basic equations,permeability of various capillary beds, cells and intracellularinclusions, bioelectric phenomena, secretion and distributionvolumes. Thor.opson.

Renal Electrolyte Excretion (2 credits). Current concepts of nephronfunction will be discussed with particular emphasis on electrolytetransport. The course will consist mainly of lectures, but oralreports on selected literature may be required. Subjects to becovered to a varying extent may include methods, site and natureof electrolyte transport mechanisms, countercurrent mechanism,water reabsorption, acid-base regulation, hormonal influence onkidney, regulation of renal blood flow. Sullivan.

Fluid, Electrolyte, and Acid-Base Metabolism (3 credits).Methods of determining body fluid compartments, fixed cation andanion balance, basic concepts of hydrogen ion balance, buffersystems of the body, intracellular hydrogen ion activity, inter-action of these factors on each other. The course will be taughtprimarily from the quantitative approach with problem solvingas an important feature. Brown.

Physiology of the Nervous System (3 credits). Selected topics incurrently active neurophysiological problem s and establishedconcepts. Lecture, laboratory, conference, and student reports.Physiology of the central, rather than the peripheral, nervoussystem will be emphasized. The sterotaxic instrument will be

Annual Report -Dept. Physiol.

KUMCI July 11 1963 -June 30 I 64Page 4

used for placement of lesions, drugs, and electrodes (stimulatingand recording). Electronic instrumentation will be used ex-clusively. Prerequisite, 271. Loofbourrow.

325. Biology of Sex (3 credits). This course will be a series of lecturesand seminars dealing with the anatomy, genetics, embryology, endo-crinology and, to a minor extent, psychobiology of sex. Theclassical experiments on lower forms will be covered, as well asclinical problems related to congenital and endocrine based sexanomalies in man. Johnson.

350. Research (1-15 credits). Original laboratory investigation conductedunder the supervision of a senior staff member. Staff.

351. Seminar (1-4 credits). Student participation conferences in whicha sharply delineated field isexamined in depth largely throughcritical review of current literature in the field. The subjectsexamined in these seminars are dictated by the interests ofstudents and staff. Staff.

360. Thesis (1-15 credits).accepted. Staff.

Credits given only after the thesis has been

RESEARCH

Research grants which were active during the year were the following:

Ventilatory responses to chemical stimuli in arterial blood. Air Forcecontract #AF 41-(657)-400, USAF School of Aerospace Medicine, Brooks AirForce Base, Texas. Principal Investigator: E. B. Brown, Jr. August 1,1961 to July 31, 1964. $27,400.

Potassium metabolism and acid-base balance. USPHS #5649.Principal Investigator: E. B. Brown, Jr. June 1, 1963 - May 31, 1964,$12,789.00. June 1, 1964 - May 31, 1965, $16,560.

A study of cardiovascular regulatory mechanisms. USPHS #H-3360(PHY). Principal Investigator: E. B. Brown, Jr. Co-investigator:C. Frederick Kittle. May 1, 1962 - November 30, 1963, $38,980, andDecember 1, 1963 - November 30, 1964, $11,800.

Tissue CO2 chemical forms and exchange rate. USPHS #GM-10429-01.Principal Investigator: Alan M. Thompson. September 1, 1963 - August31, 1964, $17,250.

Annual Report-Dept. Physiol.KUMC, July 1, 1963 - June 30, i 64Page 5

Control of collecting duct secretion of hydrogen and potassium. NSF#GB-655. Principal Investigator: Lawrence P. Sullivan. January 1, 1963-December 31, 1964. $16,900.

Hydrogen Ion Retention in Diabetic Acidosis and Ketosis. USPHS#AM-06570 -0 2. Principal Investigator: Virginia Tucker. Co -investigator:E. B. Brown, Jr. September 1, 1963 - August 31, 1964. $12,328.

Effect of pH upon renal excretion of weak acids and bases. USPHS#HE-0697 4-03. Principal Investigator: Lawrence P. Sullivan. January1, 1963 - December 31, 1963. $9,487.

In vivo blood buffer curves. Kansas Heart Association #63R 1218.

Principal Investigator: E. B. Brown, Jr. July 1, 1963 to June 30, 1964.$3,400.

Intracellular pH in endogenous and exogenous acidosis. USPES#2-F2-HE-ll 235-03. Principal Investigator: E. B. Brown, Jr. July 1,1963 - June 30, 1964, $500. (Postdoctoral Fellow Award for Dr. Tucker,)

The effect of changes in right atrial pressure upon the heart rate.Institutional Grant #589-124-4100189. Dr. K. L. Goetz. January 1, 1964 -December 31, 1964, $2,000.

Control of collecting duct secretion of Potassium and Hydrogen.Institutional Grant #589-124-4100510. Principal Investigator: Dr. SulHvan,March 15, 1964 - December 31, 1964, $3,500.

The Hepato Toxicity of anesthetic agents. Institutional Grant#589 -124-4100517. Principal Investigators: Alan M. Thompson and JamesEvans. November 1, 1963 - 12/31/64. $2,380.

Distribution of sucrose and plasma protein. American MedicalAssociation. (Medical Center #1l5-E) Principal Investigator: Dr, AlanThompson, $1,000.

Fellowship Award, Public Health #1-Fl-GM.-20, 339-01. PrincipalInvestigator: Alan M. Thompson. Fellow: Robert E. Thurber. June 1,1963 - May 31, 1964, $7,000.

Fellowship Award, Public Health #1-Fl-GM-16, 493-01A1. PrincipalInvestigator: E. B. Brown, Jr. Fellow: Robert W. Hamilton, Jr. July1, 1963 - June 30, 1964. $6,700.

Annual Report -Dept. Physiol.KUMC, July 1, 1963 -June 30, I 64

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Fellowship Award, Public Health #5-FI-GM-13, 199-04. PrincipalInvestigator: E. B. Brown, Jr. Fellow: Richard L. Clancy. January 15,1964 - January 14, 1965. $5,700.

Right atrial pressure and heart rate. USPHS #lROIHE 08803-01 (PHY)Principal Investigator: Dr. Kenneth Goetz. June 1, 1964 - May 31, 1965.$14,417.

Motor effects of the arrest reaction. USPHS #MH 08908-01 EP lRO!.

Principal Investigator: Dr. G. N. Loofbourrow. June 1, 1964 - May 31,1965. $15,582.

Experimental production of pulmonary hypertension. PHS #HE-04392-04.Principal Investigator: C. Frederick Kittle. October 1, 1963 - September30, 1964, $8, 592.

Fellowship Award, Public Health #1-F2-GM-2l, 994-01. Circulatoryadjustments of hepatic hemodynamics under anesthesia. PrincipalInvestigator: Dr. E. L. Fredrickson and Dr. A. M. Thompson. Fellow:Dr. James A. Evans. October 1, 1963 - September 30, 1964, $8,500.

During the summer of 1963 the following students engaged in researchprojects and received research experience in the department:

Murray Corbin, Jerry Albright, Douglas Wilmore, Herbert Hilgers,Ramon Schmidt, Ralph Kauffman, Alan Moorhead, Merrill Stiles,Larry Benson, Susan Black, Terry Sutton, Jay Richardson, TomDarrah, Bart Lindsley, and Janice Fleming. Kaw Valley HeartSummer Search Talent sponsored Steve Rosenbaum and JackStrandmark.

PUBLICATIONSPublications by members of the staff during the period of July 1, 1963 -

June 30, 1964:

Hamilton, R. W., Jr. and E. B. Brown, Jr. Variation in respiratoryresponse to CO2 at different constant levels of blood pH. Fed. Proc.23:259, 1964 (Abstract).

Pekas, Jerome C., Virgil W. Hays and Alan M. Thompson. Exclusion ofthe exocrine pancreatic secretion: Effect on digestibility of soybeanand milk protein by baby pigs at various ages. The Journal ofNutrition, 82:277 -286, 1964.

Annual Report-,:: Dept. Physiol.KUMC, July I, 1963 - June 30, , 64Page 7

Hamilton, R. W., Jr., R. F. Hustead, andL. F. Peltier. Fat embolism:The effect of particulate embolism on lung surfactant. Surgery,56:53, 1964.

Hamilton, R. W., Jr. The mechanics of Breathing. Interceptor (AirDefence Command; USAF) 5(9) :16-19, September, 1963.

Hamilton, R. W., Jr., and E. B. Brown, Jr. Interaction of PC02 and H+as respiratory Stimulants. Fed. Proc., 23:259, 1964.

Johnson, D. C. and E. Witschi. Hypophyseal gonadotropins followinggonadectomy in male and female androgenized rats. ActaEndocrinology, 44:119-127, 1963.

Johnson, D. C. and E. Witschi. Response of X-irradiated and cryptorchidtestes to endogenous gonadotropins in parabiotic rats. J. Endo-crinology' 73:467-474, 1964.

Johnson, D. C., M. Yasuda, B. N. Sridharan. Prepuberal development ofthe androgenized male rat. J. Endoc rinology, 29:95-97, 1964.

Johnson, D. C. Preparation of a luteinizing hormone releasing factorfrom sheep hypothalamus. Experientia 20:3ll-~, 1964.

Johnson, D. C. Production and release of hypophyseal LH by hypothalamicextracts in prepuberal male rats. Anat. Rec. 148:294-295, 1964(Abstract of paper given at the 77th session of the American Assoc.of Anatomists).

Sullivan, L. P. Effect of urine and blood pH on renal ammonium excretionduring stopped flow. The Physiologist, 6:281, 1963.

Razon, B. M. and L. P. Sullivan. Effect of pH on Renal Nitrofurantoinexcretion during stopped flow. The Physiologist, 6:261, 1963.

Schmidt, R. W. and L. P. Sullivan. Effect of Meralluride on distalnephron transport of Na, Cl and K. Fed. Proc., 23:307, 1964.

Evans, James A., and Alan N:. Thornpson. Circulatory adjustments ofhepatic hemodynamics under anesthesia. (Abstract presented forpublication to Am. Soc. of Anesthesiologists.)

Annual Report.. Dept. Physiol.KUMC, July I, 1963 -June 30, '64Page 8

Tucker, Virginia L., Hugh Hanna, Carolyn J. Kaiser, and Daniel C. Darrow.Cardiac necrosis accompanying potassium deficiency and adminis-tration of corticosteroids. Circulation Research, 13:420, Nov. 1963.

VISITORS AND OUTSIDE SPEAKERS:

Several outside speakers were brought into the department duringthe year. Institutional Research Grant funds paid expenses and honorariafor such speakers. These included:

1/10/64

2/21/64

3/26/64

4/27/64

6/22/64

Dr. Daniel H. Simmons of Cedars of Lebanon, Mt. SinaiHospitals, "Acid-Base Changes and Pulmonary VascularResistance. "

Dr. Eugene Grim, Dept. of Physiology, University ofMinnesota School of Medicine, "The Application ofIrreversible Thermodynamics in Biological Transport. "

Dr. R. D. B. Dill, National Res. Found. Scholar, Dept.of Anatomy and Physiology, Indiana University, "Adaptationto high Altitude as Related to Age. "

Dr. Gerhard Giebisch, Assoc. Prof. of Physiology, CornellUniversity Medical College, "Renal Tubular Transport ofNa and K as Studied on Single Nephrons. "

Dr. John M. Brookhart, Professor of Physiology andChairman of Department of Physiology, University ofOregon Medical School, "Function For Dendrites. "(Dr. Brookhart is also President Elect of theAmerican Physiological Society.)

ACTIVITIES OF STAFF:

Dr. Brown:

Editor: Proc. Soc. Exp. Biol. and MedicineModern Medicine.

Member NIB Physiology Fellowship Panel.

Attended invited conference on "Oxygen Transport", held tohonor Dr. Roughton, UCLA, July, 1963.

Principal speaker, Univ. of Kansas, Lawrence, for ScienceDay, October, 1963.

Annual Report -Dept. Physiol.KUIVC, July 1, 1963 -June 30, ! 64

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Delivered two lectures at Temple University Medical School,Philadelphia, Pennsylvania, January, 1964.

Member of Faculty, Basic Science Refresher Course, Am.College of Physicians Meeting, Atlantic City, May, 1964.

Participated in several programs sponsored by the PostgraduateMedical Education Department of KUMC. Circuit course, KUMC,December, 1963, and January, 1964.

Participated in the Alumni Day Program in the month ofMay, 1964.

Served as Consultant in Research, VA Hospital, Wadsworth, Kansas.

Dr. L. P. Sullivan:Gave lecture for Postgraduate Course, KUMC, Nov., 1963.

Presented paper at Fall meetings, Am. Physiol. Soc., Miami,Florida, August 1963.

Dr. Benjamin Razon, Postdoctoral Fellow, presented paper atFall meetings, APS, Miami, Florida, August, 1963. Dr. Sullivanwas coauthor on paper.

Lectured at VA Hospital, Wichita, Kansas, January, 1964.

Raymond Schmidt, Summer Fellow and Medical Student, gave apaper on work he had carried out with Dr. Sullivan, on ResearchDay, KUMC, 1964. Mr. Schmidt also gave a paper at the FASEBmeeting in Chicago, Illinois, April, 1964.

Dr. A.. M. Thompson:Review Editor for some papers submitted to "Science".

Member of KUMC Research Committee from September 1963 to present,

Herbert (Bart) Lindsley, Summer Fellow and Med. Student gave a

paper on work done with Dr. Thompson at KUMC Research Day, 1964.

Associated with papers and presentations listed elsewhere forDr. James Evans.

Dr. Loofbourrow:Presented lecture before the Kansas City Neurological Society,

September, 1963.

Annual Report - Dept. Physiol.KUMC, July I, 1963 -June 30I 64Page 10

Dr. Kenneth Goetz:

Delivered lecture before Kansas Nursing As sodation Convention,Pittsburg, Kansas, January, 1964.

Lectured to the staff at Osowatomie State Hospital, Osowatomie,Kansas, in January of 1964.

Dr. C. F. Kittle:

A paper was presented at the Society of Vascular Surgery in June,1964, at San Francisco, authored by Dr. C. F. Kittle, H. Aoki,and E. B. Brown, Jr. The article will be published in Surgery, 1964.

Dr. Evans:

Presented a paper to Midwest Anesthesiology Residents Meeting on"The Effects of Chloroform and Ether on Hepatic Hemodynamics, "May of 1964.

Student Research Day at KUMC - read paper on liThe Acute Effectsof chloroform and ether on hepatic hemodynamics. II

During the year an application was made to the NIH for a TrainingGrant in Physiology. A site visit was made by Drs. James Dow, LorenCarlson, and John Brookhart, in December of 196-3. Approval of theTraining Grant for $30,000 for the first year, and $36,000 for the nextfour years was received in May, 1964. The grant, to begin July 1, 1964,provides money for stipends for graduate students, and 600/0 of the salaryfor an Assistant Professor. Dr. John Trank has accepted our offer ofthis position and will join our staff in August, 1964.