annual report dick / slabs rcas, academia sinica
DESCRIPTION
Annual Report DiCK / SLaBS RCAS, Academia Sinica. PI: Kuo Kan Liang 梁國淦. Theme of Researches. Chemical reactions and transport processes in biological systems Special emphases: Membrane protein ion channels Methodology: Spectroscopic methods Electrochemistry methods - PowerPoint PPT PresentationTRANSCRIPT
RCAS, AS and BST, NTU
PI: Kuo Kan Liang 梁國淦
Annual ReportDiCK / SLaBS
RCAS, Academia Sinica
RCAS, AS and BST, NTU
Theme of Researches
Chemical reactions and transport processes in biological systems– Special emphases: Membrane protein ion channels
Methodology:– Spectroscopic methods– Electrochemistry methods– Both include theory and experiments
RCAS, AS and BST, NTU
Methodology:Spectroscopic Methods
Eternal goal: Spectroscopic observation of the kinetics with as little labeling as possible
IR spectroscopy;– Mid-IR range observation of amide-I peaks– Ultrafast multiphoton coherent spectroscopy such
as 2D-IR– Time-resolved FT-IR
Fluorescence spectroscopy:– Natural amino acids: Tryptophan, tyrosine
RCAS, AS and BST, NTU
Methodology:Spectroscopic Methods
Protein backbone modes couplings can be observed as vibration coherence as well as mode anharmonicity
Past achievements– Theory of anharmonic effect on absorption lineshape– Theory of effect of near-by vibronic level on the 3-
pulse photon echo signal of a vibration mode– Theory of anharmonic and mode-mode coupling
effect on the 3-pulse photon echo signal of a vibration mode.
RCAS, AS and BST, NTU
Publications:Spectroscopic Methods
Chu, Z.*; Liang, K. K.*; Hayashi, M.; Lin, S. H., “Theoretical treatment of anharmonic effect on molecular absorption, fluorescence spectra, and electron transfer”, Chem. Phys. 2009, 358, 137.
RCAS, AS and BST, NTU
Publications:Spectroscopic Methods
Villaeys, A. A.*; Dappe, Y.; Liang, K. K.; Lin, S. H., “Influence of neighboring levels in three-pulse photon-echo processes”, Phys. Rev. A 2009, 79, 053418. (Virtual Journal of Ultrafast Science 2009, Vol. 8, Issue 6)
RCAS, AS and BST, NTU
Publications:Spectroscopic Methods
Liang, K. K.*; Villaeys, A. A., “Anharmonicity and mode-mode coupling effects analyzed by 4WM photon-echo spectroscopy”, Phys. Rev. A 2010 (Accepted with minor corrections).
Methodology:Electrochemistry Methods
Impedance spectroscopy (transfer function) of ion channels
Modified electrophysiology (patch-clamp) experiments– Patch-clamp impedance spectroscopy– Patch-Clamp fluorometry (Electrophysiology +
Spectroscopy)– Time-resolved experiments: synchronization method
RCAS, AS and BST, NTU
Publication:Electrophysiology Methods
Chang, H.-K.; Martin, L. J.; Liang, K. K.; Shieh, R.-C.*, “K+ binding in G-loop and water cavity facilitates Ba2+ movement in the Kir2.1 Channel”, Biochim. Biophys. Acta 2009, 1788, 500.
Ba2
+bl
ocki
ng t
ime
- 120 mV
+ 40 mV
Ba2+ Blocking recovery time
Sec
ond
test
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se
Ba2
+bl
ocki
ng t
ime
Sec
ond
test
pul
se
0 mV
short longMembrane voltage
Hill plot:Hill coefficient ~ 2.5Indicating that cooperative binding is not the sole story
RCAS, AS and BST, NTU
Further Developments:Short term
Theory of kinetics of cooperative systems * Stochastic model of ion transport in channels * Molecular dynamics simulations and analysis of
hydrated cations transport in bulk and in artificial channels
Patch-clamp fluorometry of unlabeled Kir 2.1/ 2.2 channels
RCAS, AS and BST, NTU
Further Developments:Mid-term
Impedance of ion channels– Theoretical method: Chemical kinetics simulation of
the ion transport process in ion channels– Experimental method: AC patch-clamp
Advanced materials for CO2 separation– Theory of CO2 or CO3
2– transport in pores such as in polymer-metal framework (PMF)
– Model system construction for cell-free CO2 reduction using enzymes
RCAS, AS and BST, NTU
Further Developments:Long-term
Thermodynamics and kinetics of single- / few- biological-macromolecule events– Theory of reactive transport in pores / channels– Thermodynamics and kinetics of binding in channels– Thermodynamics systems with electrostatic works
Electrochemical and structural kinetics of cells– Synchronous observations of reactions, transport
processes, and structural changes on membrane– Engineering of cellular or cell-free molecular
factories