antibacterial property of cayenne pepper

7/23/2019 Antibacterial Property of Cayenne Pepper

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INTRODUCTION

The price of medicine today has become very expensive for

the people who earn average income a month. etting sic! is a

big strain to their b"dget# so they wo"ld see! for

alternatives that are affordable and more effective. $any

researchers contin"e to st"dy several plants that co"ld be an

alternative to expensive medicine b"t are more effective.

Cayenne %epper is m"ch !nown in o"r co"ntry especially to

those who love spices. It is relatively high in vitamin &.

'erbal medicines have very high potential in the field of

medicine. &side from being cheap# it can be easily ac("ired by

anyone. These herbal medicines have also the same effects with

the commercial medicines. This st"dy is done to test the

effectiveness of Cayenne %epper leaves extract as an

antibacterial.

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)tatement of the %roblem

The st"dy aims to test the antibacterial property of

Cayenne %epper *Capsic"m ann"m+ leaves and compared the

res"lts with the commercial antibiotic.

,. Is there any anti-bacterial property fo"nd in Cayenne

%epper leaves extract/. Is there any significant difference between Cayenne

%epper leaves extract as anti-bacterial agent compared to

the commercial one

)tatement of the 'ypothesis

N"ll 'ypothesis0

'o,. There is no anti-bacterial property fo"nd in Cayenne

%epper leaves extract.

'o/. There is no significant difference between Cayenne %epper

leaves extract as anti-bacterial agent compared to the

commercial one.

&lternative 'ypothesis0

'a,. There is anti-bacterial property fo"nd in Cayenne %epper

leaves extract.

'a/. There is significant difference between Cayenne %epper

leaves extract as anti-bacterial agent compared to the

commercial one.

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)ignificance of the )t"dy

In o"r modern generation we all !now that there are many

diseases exist wherever we go and affect people.The st"dy of

Capsic"m ann"m is important beca"se it has many benefits and

"ses and this st"dy aims to determine if Cayenne %epper leaves

extract has any antibacterial property against ). marcescens

and 1. s"btilis and aims to help people find new c"re to their

diseases and to discover and develop more "sef"l things and

co"ld treat illness with Cayenne %epper leaves extract which

is a less expensive s"bstit"te.

)cope and 2imitations

This st"dy foc"ses only on the "se of Cayenne %epper

leaves extract. The commercial antibiotic "sed in this st"dy

is &moxicillin. The proced"re was all done in the Research

laboratory at "sa Regional )cience 'igh )chool-3. The st"dy

was done with proper handling of materials and r"les were

followed "pon cond"cting the st"dy.

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Definition of Terms

Cayenne Pepper 4 a p"ngent hot-tasting red powder

prepared from gro"nd dried chili peppers

Agar4 a 5elly-li!e s"bstance# obtained from algae Autoclave4 press"re chamber "sed to sterili6e e("ipment

and s"pplies

Bacillus Subtilis 4 a ram-positive# catalase-positive

bacteri"m# fo"nd in soil and the gastrointestinal tracts

of r"minants and h"mans

Serratia Marcescens 4 a species of rod-shaped ram-

negative bacteria in the family 7nterobacteriaceae. It

may be fo"nd in environments s"ch as dirt# s"pposedly

8sterile9 places# and the s"bgingival biofilm of teeth

R7:I7; O< R72&T7D 2IT7R&TUR7

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Cayenne pepper is a hot chili pepper# fre("ently "sed in

the preparation of spicy dishes that has been associated with

a wide range of health benefits. The chili originated in

Central and )o"th &merica. It is named after the capital city

of the


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Cayenne is a pop"lar spice in a variety of c"isines. It

is employed vario"sly in its fresh form# dried and powdered#

and as dried fla!es. It is also a !ey ingredient in a variety

of hot sa"ces# partic"larly those employing vinegar as a

preservative. Cayenne pepper is often spread on sandwiches or

similar items to add a spicy flavor. 1"ffalo wingsa"ce often

contains cayenne. 1everage foods are emerging with cayenne

extract as an active ingredient. &lso# cayenne pepper diet

recipehas become very pop"lar in last co"ple of years.

Cayenne pepper# by weight# is relatively high in vitamin

&. It also contains vitamin 1# vitamin 7# vitamin

C# riboflavin# potassi"m# and manganese. 'owever# given the

very small amo"nt of cayenne pepper typically cons"med in a

serving# it ma!es a negligible contrib"tion to overall dietary

inta!e of these n"trients. Cayenne pepper is also claimed to

be an aphrodisiacbeca"se it contains capsaicin. Capsaicin has

been shown to increase energy expendit"re# so acts as a

metabolism booster and is beneficial in long-term weight loss.

Capsaicin treatment s"stained fat oxidation d"ring weight

maintenance# b"t did not affect weight regain after modest

weight loss. It has also been shown to aid in

theoxidationof adipose tiss"e# reg"late high blood press"re#

promote healthy liver f"nction and tiss"e prod"ction# help

reg"late the digestive system# and promote healthy m"c"s

prod"ction in the membranes that line internal organs.

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https://en.wikipedia.org/wiki/Buffalo_winghttp://www.gcfofguide.com/cayenne-pepper-diet-the-fast-one/http://www.gcfofguide.com/cayenne-pepper-diet-the-fast-one/https://en.wikipedia.org/wiki/Vitamin_Ahttps://en.wikipedia.org/wiki/Vitamin_Ahttps://en.wikipedia.org/wiki/Vitamin_B6https://en.wikipedia.org/wiki/Vitamin_B6https://en.wikipedia.org/wiki/Vitamin_Ehttps://en.wikipedia.org/wiki/Vitamin_Chttps://en.wikipedia.org/wiki/Vitamin_Chttps://en.wikipedia.org/wiki/Riboflavinhttps://en.wikipedia.org/wiki/Potassiumhttps://en.wikipedia.org/wiki/Manganesehttps://en.wikipedia.org/wiki/Aphrodisiachttps://en.wikipedia.org/wiki/Oxidationhttps://en.wikipedia.org/wiki/Adipose_tissuehttps://en.wikipedia.org/wiki/Buffalo_winghttp://www.gcfofguide.com/cayenne-pepper-diet-the-fast-one/http://www.gcfofguide.com/cayenne-pepper-diet-the-fast-one/https://en.wikipedia.org/wiki/Vitamin_Ahttps://en.wikipedia.org/wiki/Vitamin_Ahttps://en.wikipedia.org/wiki/Vitamin_B6https://en.wikipedia.org/wiki/Vitamin_Ehttps://en.wikipedia.org/wiki/Vitamin_Chttps://en.wikipedia.org/wiki/Vitamin_Chttps://en.wikipedia.org/wiki/Riboflavinhttps://en.wikipedia.org/wiki/Potassiumhttps://en.wikipedia.org/wiki/Manganesehttps://en.wikipedia.org/wiki/Aphrodisiachttps://en.wikipedia.org/wiki/Oxidationhttps://en.wikipedia.org/wiki/Adipose_tissue


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)C'7$&TIC DI&R&$

Independent :ariable Dependent :ariable

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7xtraneo"s :ariable


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The materials that were "sed in the experiment are

Cayenne pepper leaves extract# triple beam balance# ,?? m2 of

>EF ethanol# G petri dishes# stirring rod# alcohol lamp#

s"rgical gloves# matchstic!s# a"toclave# inoc"lating hood#

,./ g of n"trient agar# B?? m2 of p"rified water# /? paper

disc# &moxicillin caps"le# bea!er# mas!# p"ller# and

sterili6ed paper.

%roced"re

Gathering of materials. ather all the materials

needed in performing the experiment# )panish %l"m )tem# triple

beam balance# petri dish# stirring rod# alcohol lamp# s"rgical

gloves# match stic!s# cotton swabs# a"toclave# inoc"lating

hood# n"trient agar# triple distilled water# paper disc# anti-

bacterial caps"le# bea!er# mas!# p"ller and paper for

wrapping.

Weighing/Air drying/ Soaing.The c"t plant was p"t in a

folded paper and weighed ,>./g and then air-dried for one

wee!. &fter air-drying for one wee!# weigh it again. &fter air

drying# the c"t plant was soa!ed in ,??ml of >EF ethyl alcohol

and soa!ed for six days.

Sterili!ation. )terili6e the %etri dishes# cotton swabs#

mas!s# gloves# ,,.B dissolved n"trient agar with B??ml of

triple distilled water# paper discs# p"ller and other

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materials inside an a"toclave at maxim"m ,/,oC for ,B min"tes

and another ,B min"tes for cooling. Then label the / petri

dishes.

"noculating. It is where the c"lt"ring of bacteria occ"rs

and where the two bacteria are placed. These are ). $arescens

and 1. )"btilis. This is done in an inoc"lating hood.

S#abbing. The coo!ed n"trient agar was then po"red into

the / petri dishes. &fter how many min"tes p"t the ,? paper

disc into the p"re extract and ,? into the commerciali6e one.

Using the mas! and the gloves# sterili6e the / petri dishes

"nder the alcohol lamp and caref"lly dip the cotton swabs into

the bacteria and gently swab the cotton into the n"trient

agar. Using p"ller# p"t B paper disc on each replicate. B for

the p"re extract and another B for the commerciali6e one.

Cover and heat again the petri dishes and wrap it with

sterili6ed paper. 2abel the wrapped petri dishes and do this

all again to other bacteria.

"ncubation. The wrapped %etri dishes will be inc"bated in

the inoc"lating hood within GE ho"rs.

$bservation. ;ith the help of yo"r Research teacher#

observe and meas"re the 6one of inhibition in millimeters of

each treatment "sing a r"ler and record it in / separate

tables.

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Waste disposal. ;ash all the laboratory apparat"ses and

p"t them in the a"toclave for decontamination. )ome materials

will be incinerated to avoid environmental contamination.

)tatistical Tool

In this experiment# the researcher "sed the T-test on

independent variables to !now the significance between the

means of comparisons.

T-test

d

2

n d2

Tc=d

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R7)U2T) &ND DI)CU))ION)

Table ,. Hone of inhibition *in mm+ "sing ). marcescens

Treatments

Replicates

Total $eanR, R/ RA RG RB

T, *Cayenne

pepper leaves

extract

,E /? , // /? > ,>./

T/

*Commercial+

,/ , ,? ,/ ,/ / ,/.G

The table shows the 6one of inhibition on the 1.

marcescens of the treatment , which has the mean of ,>./ mm

and treatment / which has the mean of ,/.G mm. It shows that

treatment , has higher mean than treatment /.

Table /. Hone of inhibition *in mm+ "sing 1. s"btilis

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Treatments

Replicates

Total $eanR, R/ RA RG RB

T, *Cayenne

pepper leavesextract

,? ,/ ,G ,/ ,? BE ,,.

T/

*Commercial+

,E , /? , // >/ ,E.G

The table shows the 6one of inhibition on the 1. s"btilis

of the treatment , which has the mean of ,,. mm and treatment

/ which has the mean of ,E.G mm. It shows that treatment , has

lesser mean than treatment /.

CONC2U)ION &ND R7CO$$7ND&TION

Concl"sion

The mean val"e of Treatment , is greater than the mean

val"e of Treatment / in Table , *T, ,>./# T/ ,/.G+ and Table

/ *T, ,,.# T/ ,E.G+ respectively. The test statistic in

table ,# tc.E is greater than the critical val"e /.A, th"s

the researcher re5ect n"ll hypothesis. This implies that there

is a significant difference between the antibacterial property

*against ). marescens+ of Cayenne pepper leaves extract and

&ntibiotic &moxicillin. The test statistic in table /# tc-

G.BGA is lower than the critical val"e /.A, th"s the

researcher accepted n"ll hypothesis. This implies that there

is no significant difference between the antibacterial

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property *against ). marescens+ of Cayenne pepper leaves

extract and &ntibiotic &moxicillin.

Recommendation


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1I12IOR&%'

$NT. ;hat are the benefits of Cayenner */?,G# )eptember E+.

Retrieved from

http0JJwww.medicalnewstoday.comJarticlesJ/K/GE.php

;i!ipedia. */?,B# L"ne /E+ Cayenne pepper. Retrieved from

https0JJen.wi!ipedia.orgJwi!iJCayenneMpepper

Cayenne pepper n"trition facts and health benefits. */??>+

Retrieved from http0JJwww.n"trition-and-yo".comJcayenne-

pepper.html

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http://www.medicalnewstoday.com/articles/267248.phphttps://en.wikipedia.org/wiki/Cayenne_pepperhttp://www.nutrition-and-you.com/cayenne-pepper.htmlhttp://www.nutrition-and-you.com/cayenne-pepper.htmlhttp://www.medicalnewstoday.com/articles/267248.phphttps://en.wikipedia.org/wiki/Cayenne_pepperhttp://www.nutrition-and-you.com/cayenne-pepper.htmlhttp://www.nutrition-and-you.com/cayenne-pepper.html


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&%%7NDI3 &

T&127 ,. Hone of inhibition *in mm+ "sing ). marcescens

T/

*Commercial+

T,

*)panish %l"m

)tem 7xtract+

Dk Dk

2

,/ ,E A, /? G ,,? , A,/ // ,? ,??,/ /? E G

T2=62 T1=96 Total AG Total /B/

d

2

n d2

Tc=d

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Tc=

3 4

5 (252 )(34 )

(51)

Tc .E

df ,?-/E

level of significance BF or ?.?B

tcrit=2.31

tcalc < t(0.05)(8)= 2.31

T&127 /. Hone of inhibition *in mm+ "sing 1. s"btilis

T/

*Commercial+

T,

*)panish %l"m

)tem 7xtract+

Dk Dk

2

,E ,? -E G, ,/ -G ,

/? ,G - A, ,/ -G ,// ,? -,/ ,GG

T2=92 T1=58 Total -AG Total /K

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d

2

n d2

Tc=d

Tc=

34

5 (276 )(34)

(51)

Tc -G.BGA

df ,?-/E

level of significance BF or ?.?B

tcrit=2.31

tcalc < t(0.05)(8)= 2.31

LOURN&2

D&T7 %ROC7DUR7

LUN7 ,/# /?,B The researcher gathered

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)panish %l"m )tem

LUN7 ,G# /?,B ;eighing of the stem# c"t

them into tiny bits# first

day of air-drying

LUN7 ,B# /?,B 7xtraction of 7xtract

LUN7 /,# /?,B )wabbing and Inc"bation

LUN7 /A# /?, athering of Data

LUN7 /># /?,B Oral Defense

LU2 ,A# /?,B )"bmission of Research %aper

for 7diting

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antibacterial property of cayenne pepper

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