antibiotics analysis by hplc - ナカライテスク · pdf fileantibiotics analysis by hplc...

Technical Note

Antibiotics are widely used in food-producing animals to prevent disease or promote growth. The use of antibiotics may result in drug residues in the animal derived food. The analysis of antibiotics residues in animal derived food is important worldwide to ensure foods safety. Excellent High Per-formance Liquid Chromatography (HPLC) analyses of these substances can be achieved by using COSMOSIL columns.

(1) Fluoroquinolones

• LC/MS analysis by COSMOSIL 3C18-EB Columns • HPLC Analysis by COSMOSIL 5C18-MS-II

COSMOSIL Application DataCOSMOSIL Application Data

Column: 3C18-EBColumn size: 2.0mmI.D.-150mmMobile phase: 0.1% Formic Acid-Acetonitrile/ H2O = 15/85Flow rate: 0.2 ml/min Temperature: 40°CDetection: ESI-MS, Positive, SIM

Sample: 1; Ciprofloxacin (21mg/L) 2; Danofloxacin (25mg/L)3; Enrofloxacin (25mg/L)4; Sarafloxacin (25mg/L)Inj.Vol.: 1.0 l

NACALAI TESQUE, INC

COSMOSIL Application DataCOSMOSIL Application DataColumn: 5C18-MS-IIColumn size: 4.6mmI.D.-150mmMobile phase: Methanol/ 20mmol/l Phosphate buffer(pH3) = 55/45Flow rate: 1.0 ml/min Temperature: 30oCDetection: UV270nm, 0.16AUFS

Sample: 1; Ofloxacin (0.39 μg) 2; Oxolinic Acid (0.08 μg) 3; Flumequine (1.08 μg) 4; Piromidic Acid (0.08 μg)

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(2) Aminoglucosides

• Simultaneous LC/MS analysis by COSMOSIL 3C18-EB


Column: 3C18-EBColumn size: 2.0mmI.D.-150mmMobile phase: 0.1% Heptafluoro-n-butyric Acid -Acetonitrile/ H2O = 30/70Flow rate: 0.2 ml/min Temperature: 40°CDetection: ESI-MS, Positive, SIMSample: 1; Spectinomycin 2; Hygromycin B 3; Streptomycin 4; Dihydrostreptomycin 5; Amikacin 6; Kanamycin 7; Apramycin 8; Tobramycin 9; Gentamicin 10; Neomycin Inj.Vol.: 1.0 l NACALAI TESQUE, INC

Antibiotics Analysis by HPLC

(2) Aminoglucosides

• LC/MS analysis of Gentamicin by COSMOSIL PBr


NACALAI TESQUE, INC

Column: PBrColumn size: 2.0mmI.D.-150mmMobile phase: 0.1% Pentafluoropropionic Acid -Acetonitrile/ H2O = 10/90Flow rate: 0.2 ml/min Temperature: 40°CDetection: ESI-MS, Positive, SIM

Sample: Gentamicin (C1a, C2, C2a, C1)Inj.Vol.: 1.0 l

(3) Sulfonamides

• HPLC Analysis (COSMOSIL 5C18-MS-II vs. COSMOSIL Cholester)


Column: Column size: 4.6mmI.D.-150mmMobile phase: A; 0.1% Formic Acid-H2O B; 0.1% Formic Acid-Acetonitrile B conc. 10→50% 20min Linear gradient Flow rate: 1.0 ml/min Temperature: 40oCDetection: UV254nm

Sample: 1; Trimethoprim (0.1mg/ml) 2; Sulfadiazine (0.1mg/ml) 3; Sulfathiazole (0.1mg/ml) 4; Sulfamerazine (0.1mg/ml) 5; Sulfamethazine (0.1mg/ml) 6; Sulfachloropyridazine (0.1mg/ml) 7; Sulfamethoxazole (0.1mg/ml) 8; Sulfadimethoxine (0.1mg/ml)Inj.Vol.: 1.0 l NACALAI TESQUE, INC

5C18-MS-II Cholester

• LC/MS Analysis by COSMOSIL 2.5Cholester


NACALAI TESQUE, INC

Column: 2.5CholesterColumn size: 2.0mmI.D.-50mmMobile phase: A; 0.1% Formic Acid-H2O B; 0.1% Formic Acid-Acetonitrile B conc. 10→50% 5min Linear gradient Flow rate: 0.2 ml/min Temperature: 40°CDetection: ESI-MS, Positive, SIMSample: 1; Trimethoprim (10mg/L) 2; Sulfadiazine (10mg/L) 3; Sulfathiazole (10mg/L) 4; Sulfamerazine (10mg/L) 5; Sulfamethazine (10mg/L) 6; Sulfachloropyridazine (10mg/L) 7; Sulfamethoxazole (10mg/L) 8; Sulfadimethoxine (10mg/L)Inj.Vol.: 5.0 l

(4) Beta-Lactams (Penicillins)

• HPLC nalysis by COSMOSIL 5C18-MS-II

NACALAI TESQUE, INC


Column: 5C18-MS-IIColumn size: 4.6mmI.D.-150mmMobile phase: Methanol/ 20mmol/l Phosphate buffer(pH7) = 50/50Flow rate: 1.0 ml/min Temperature: 30oCDetection: UV235nm, 0.2AUFS

Sample: 1; Carbenicillin (1.5 μg) 2; Ampicillin (3.0 μg) 3; Methicillin (1.5 μg) 4; Penicillin G (3.0 μg)

(5) Tetracyclines• HPLC Analysis by COSMOSIL 5C18-MS-II

NACALAI TESQUE, INC

COSMOSIL Application DataCOSMOSIL Application DataColumn: 5C18-MS-IIColumn size: 4.6mmI.D.-150mmMobile phase: Methanol/ 20mmol/l Phosphate buffer(pH3) = 20/80Flow rate: 1.0 ml/min Temperature: 20oCDetection: UV254nm

Sample: 1; Oxytetracycline 2; Tetracycline 3; Chlortetracycline

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(6) Lincosamides

• HPLC Analysis by COSMOSIL 5C8-MS

NACALAI TESQUE, INC

COSMOSIL Application DataCOSMOSIL Application DataColumn: 5C8-MSColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ 100mmol/l KH2PO4 (pH2.5 with H3PO4) = 22/78Flow rate: 1.0 ml/min Temperature: 25oCDetection: UV210nm

Sample: 1; Lincomycin (4.0 μg) 2; Clindamycin Phosphate (4.0 μg) 3; Methyl p-Hydroxybenzoate (0.3 μg)

Sample :PuromycinCAS No. :58-58-2Chromatogram Index No. :M-P0210

Column :COSMOSIL 5C18-MS-II

Sample :Rifamycin SVCAS No. :14897-39-3Chromatogram Index No. :M-R0013


Sample :CefadroxilCAS No. :50370-12-2Chromatogram Index No. :J-00102

Column :COSMOSIL 5C18-PAQ

Sample :CefotaximeCAS No. :63527-52-6Chromatogram Index No. :J-00161


Sample :CefsulodinCAS No. :52152-93-9Chromatogram Index No. :J-00103


Sample :CeftazidimeCAS No. :72558-82-8Chromatogram Index No. :J-00104


Sample :CeftriaxoneCAS No. :73384-59-5Chromatogram Index No. :J-00116

Column :COSMOSIL 5C18-AR-II

Sample :CephalexinCAS No. :15686-71-2Chromatogram Index No. :J-00166


Sample :Cephalothin sodiumCAS No. :58-71-9Chromatogram Index No. :M-C0046


Sample :Aztreonam 　CAS No. :78110-38-0Chromatogram Index No. :J-00112


Sample :AmoxicillinCAS No. :26787-78-0Chromatogram Index No. :J-00085


Sample :CloxacillinCAS No. :61-72-3Chromatogram Index No. :J-00048


Sample :PiperacillinCAS No. :61477-96-1Chromatogram Index No. :J-00093


Sample :PropicillinCAS No. :1245-44-9Chromatogram Index No. :M-P0173


Sample :CiprofloxacinCAS No. :85721-33-1Chromatogram Index No. :J-00147


Sample :LevofloxacinCAS No. :100986-85-4Chromatogram Index No. :J-00154


Sample :VancomycinCAS No. :1404-93-9Chromatogram Index No. :M-V0008


(7) Antibiotics' Chromatograms

Antibiotics’ chromatograms are available on COSMOSIL Application Search. Please visit our website and enter Chromatogram Index No. in Application No. box. For more information, please visit our website at http://www.nacalai.co.jp/global/cosmosil/data/05.html

• Antibiotics’ chromatograms list

Aminonucleosides

Ansamycins

Beta-Lactams (Cephems)

Beta-Lactams (Monobactams)

Beta-Lactams (Penicillins)

Fluoroquinolones

Glycopeptides

Sample :ClindamycinCAS No. :18323-44-9Chromatogram Index No. :J-00036


Sample :NitrofurantoinCAS No. :67-20-9Chromatogram Index No. :M-N0093


Sample :Amphotericin BCAS No. :1397-89-3Chromatogram Index No. :H-00123

Column :COSMOSIL HILIC

Sample :PimaricinCAS No. :7681-93-8Chromatogram Index No. :J-00034


Sample :Actinomycin DCAS No. :50-76-0Chromatogram Index No. :M-A0049


Sample :Polymyxin B SulfateCAS No. :1405-20-5Chromatogram Index No. :W-00074

Column :COSMOSIL 5C18 AR-300

Sample :SulfamethoxypyridazineCAS No. :80-35-3Chromatogram Index No. :M-S0056


Sample :SulfamonomethoxineCAS No. :1220-83-3Chromatogram Index No. :M-S0057


Sample :SulfaquinoxalineCAS No. :59-40-5Chromatogram Index No. :M-S0058


Sample :SulfisomidineCAS No. :515-64-0Chromatogram Index No. :M-S0061


Sample :SulfisoxazoleCAS No. :127-69-5Chromatogram Index No. :M-S0062


Sample :MinocyclineCAS No. :10118-90-8Chromatogram Index No. :J-00091


Sample :AnisomycinCAS No. :22862-76-6Chromatogram Index No. :M-A0125


Sample :Chromomycin A3CAS No. :7059-24-7Chromatogram Index No. :M-C0163


Sample :CilastatinCAS No. :82009-34-5Chromatogram Index No. :J-00073


Sample :FluconazoleCAS No. :86386-73-4Chromatogram Index No. :J-00152


Sample :KetoconazoleCAS No. :65277-42-1Chromatogram Index No. :J-00168


Sample :LovastatinCAS No. :75330-75-5Chromatogram Index No. :J-00150


Sample :MycostatinCAS No. :1400-61-9Chromatogram Index No. :M-M0214


Sample :TunicamycinCAS No. :11089-65-9Chromatogram Index No. :M-T0196


Sample :ValinomycinCAS No. :2001-95-8Chromatogram Index No. :S-00117

Column :COSMOSIL 5SL-II

Please see next page to see how to search at COSMOSIL Application Search.

Lincosamides

Nitrofurans

Polyenes

Polypeptides

Sulfornamides

Tetracyclines

Ohters

How to Search at COSMOSIL Application Search

Search Result COSMOSIL Application

Applications are searched by1. Sample Category2. Column Name 3. Sample Name4. CAS No., 5. Application No.6. Particle Size

Visit COSMOSIL Application Search page athttp://www.nacalai.co.jp/global/cosmosil/data/05.html

Click

Click

Click

pp

Ordering Information

COSMOSIL 5C18-MS-II Packed Column COSMOSIL 5C18-MS-II Guard Column

COSMOSIL 3C18-EB Column COSMOSIL 3C18-EB Guard Column

COSMOSIL Cholester Packed Column COSMOSIL Cholester Guard Column

COSMOSIL 2.5Cholester Packed Column

COSMOSIL 5PBr Column COSMOSIL 5PBr Guard Column

Column SizeI.D. x Length (mm) Product Number

1.0 x 50 02824-311.0 x 150 02896-012.0 x 30 05876-712.0 x 50 04355-212.0 x 100 05597-312.0 x 150 38025-912.0 x 250 05761-613.0 x 100 05458-513.0 x 150 34245-313.0 x 250 34254-114.6 x 30 34341-614.6 x 50 38017-01


4.6 x 100 38018-914.6 x 150 38019-81

4.6 x 150 3 lots set 09397-73

4.6 x 250 38020-416.0 x 150 38021-316.0 x 250 38022-2110 x 50 05789-2110 x 150 34355-9110 x 250 38023-1120 x 150 05091-4120 x 250 38024-0128 x 250 05760-71


4.6 x 10 38014-314.6 x 10 Cartridge* 38015-89

10 x 20 38016-1120 x 20 05790-8120 x 50 34371-7128 x 50 34347-01

* 3 cartridges included, needs a holder


1.0 x 150 05968-711.0 x 250 05969-612.0 x 30 08565-512.0 x 50 06352-912.0 x 100 06948-012.0 x 150 05971-112.0 x 250 05972-013.0 x 150 05973-913.0 x 250 05974-81


4.6 x 150 05976-614.6 x 150 3 lots set 07970-03

4.6 x 250 05977-5110 x 150 08011-9110 x 250 05979-3120 x 150 06088-7120 x 250 05982-7128 x 250 05985-41


4.6 x 10 05975-7110 x 20 05978-4120 x 20 05980-9120 x 50 05981-8128 x 50 05983-61


2.0 x 50 09000-012.0 x 75 09047-112.0 x 100 09048-013.0 x 50 09049-913.0 x 75 09050-513.0 x 100 09051-41


2.0 x 50 09794-212.0 x 75 09795-112.0 x 100 09796-012.0 x 150 09797-912.0 x 250 09798-813.0 x 50 09799-713.0 x 75 09800-213.0 x 100 09811-81


3.0 x 150 09814-513.0 x 250 09827-914.6 x 50 09840-014.6 x 75 09841-914.6 x 100 09842-814.6 x 150 09843-714.6 x 250 09844-61


4.6 x 10 09839-41


2.0 x 150 12392-81

4.6 x 150 12394-61

4.6 x 250 12395-51


10 x 250 12397-31

20 x 250 12398-21


4.6 x 10 12444-1410 x 20 12396-41

1406

For research use only, not intended for diagnostic or drug use.

NACALAI TESQUE, INC.Nijo Karasuma, Nakagyo-ku, Kyoto 604-0855 JAPANTELFAXWebsiteE-mail

: +81-(0)75-251-1730: +81-(0)75-251-1763: www.nacalai.com: [email protected]

antibiotics analysis by hplc - ナカライテスク · pdf fileantibiotics analysis by hplc...

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