appendix c - ars.els-cdn.com8: sspsma4.1b 51 50 50 50 50 49 100 100 85 46 46 46 46 38 37 9:...

Appendix C

1

Appendix C

Content Page

C.1. Phylogeny of duplicate Atlantic salmon proteasome amino acid sequences 2 C.2. Nucleotide sequence identity between DE Atlantic salmon proteasome subunit paralog sequences 3

C.3. Expression patterns of Atlantic salmon proteasome subunit duplicates in various normal tissues 4 C.4. Alignment of selected deduced PSMA7 and PSMA8 amino acid sequences 5

C.5. Deduced proteasome amino acid sequences used in phylogenies 8

Appendix C

2

Appendix C.1. Phylogeny of DE Atlantic salmon proteasome paralog amino

acid sequences

The evolutionary history was inferred by using the Maximum Likelihood method based on the Le_Gascuel_2008 model [1]. The tree with the highest log likelihood (-3481.0643) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter = 8.6048)). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 20 amino acid sequences. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 219 positions in the final dataset. Evolutionary analyses were conducted in MEGA7 [2]. Sequence references can be found in main text Table 1 and Appendix C.5 and hs is Homo sapiens and ss is Salmo salar. Blue sharing defines differentially expressed genes shown as downregulated with arrows.

1. Le S.Q. and Gascuel O. (2008). An Improved General Amino Acid Replacement Matrix. Mol Biol Evol 25(7):1307-1320. 2. Kumar S., Stecher G., and Tamura K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets.Molecular Biology and Evolution 33:1870-1874.

Appendix C

3

Appendix C.2. Nucleotide sequence identity between DE Atlantic salmon proteasome subunit sequences.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

1: ssPSMA8.1a 100 93 82 83 74 74 51 51 51 45 45 44 45 41 39

2: ssPSMA7.1b 93 100 81 83 73 72 50 50 51 44 44 44 45 41 40

3: ssPSMA8.2a 82 81 100 93 73 72 50 50 50 45 45 46 46 39 38

4: ssPSMA8.2b 83 83 93 100 73 72 50 50 51 47 46 46 46 39 38

5: ssPSMA7a 74 73 73 73 100 94 50 50 50 47 47 46 45 41 40

6: ssPSMA7b 74 72 72 72 94 100 49 49 49 46 46 45 45 39 39

7: ssPSMA4.1a 51 50 50 50 50 49 100 100 85 45 45 46 46 38 37

8: ssPSMA4.1b 51 50 50 50 50 49 100 100 85 46 46 46 46 38 37

9: ssPSMA4.2 51 51 50 51 50 49 85 85 100 46 46 47 46 37 37

10: ssPSMA6.1a 45 44 45 47 47 46 45 46 46 100 94 86 71 42 40

11: ssPSMA6.1b 45 44 45 46 47 46 45 46 46 94 100 85 71 41 40

12: ssPSMA6.2 44 44 46 46 46 45 46 46 47 86 85 100 72 40 40

13: ssPSMA6.3 45 45 46 46 45 45 46 46 46 71 71 72 100 39 39

14: ssPSMB6a 41 41 39 39 41 39 38 38 37 42 41 40 39 100 94

15: ssPSMB6b 39 40 38 38 40 39 37 37 37 40 40 40 39 94 100

Only open reading frame nucleotide sequences were included. Percent Identity Matrix was created by Clustal2.1.

Appendix C

4

Appendix C.3. Expression patterns of Atlantic salmon proteasome subunit duplicates in various normal tissues New name Genome name HK Spleen Gills Heart Liver Nose Gut Brain Ovary Testis Query length (bp)

PSMB6a PSMB6_Like 15,81 7,06 18,26 7,34 2,40 15,64 9,11 21,87 110,72 38,52 696

PSMB6b PSMB6 38,40 25,64 37,54 22,28 9,88 32,87 26,43 26,72 22,46 89,00 699

PSMA4.1a/-b PSMA4/-4_Like 36,11 23,65 19,94 13,30 8,79 19,70 32,25 18,52 29,96 37,74 786

PSMA4.2 PSMA4 23,65 21,15 15,78 4,01 3,34 14,03 14,87 7,41 29,41 46,07 831

PSMA6.1a PSMA6 11,44 6,07 6,25 3,64 2,23 7,84 5,71 7,24 27,78 49,66 741

PSMA6.1b PSMA6_Like 17,40 11,54 9,82 3,76 3,26 11,15 8,62 9,57 61,33 40,60 741

PSMA6.2 PSMA6_Like 20,35 14,08 18,94 9,26 6,82 20,65 20,56 11,95 18,09 42,21 741

PSMA6.3 PSMA6_Like 20,83 16,50 17,45 1,42 1,56 8,50 16,95 0,69 0,23 4,87 741

PSMA7a PSMA7_Like 9,39 6,05 8,60 0,79 0,96 6,14 14,42 1,53 0 2,36 741

PSMA7b PSMA7 38,42 38,48 14,98 2,00 1,73 3,80 9,56 0,79 0,15 4,20 729

PSMA8.1a PSMA7_Like 31,92 25,86 16,26 8,01 2,75 18,99 12,78 10,23 40,23 59,65 756

PSMA8.1b PSMA7_Like 34,54 21,36 18,56 9,26 5,74 21,97 23,91 19,05 48,69 54,49 756

PSMA8.2a PSMA7_Like 2,04 1,23 1,68 2,52 0,65 3,00 0,80 2,60 50,95 22,15 756

PSMA8.2b PSMA7 8,31 5,30 6,50 4,66 1,73 7,64 8,60 9,31 29,41 35,88 756

Total # reads 59084708 60203316 59793962 58163180 58784272 59545012 59806348 58939250 35803964 185311952

Transcriptional values are given in RPKM (Reads Per Kilo base per Million mapped reads). Mapping reads back to Atlantic salmon tissue reference transcriptomes was done with the CLC v 6.0.5 software. Reads

were mapped with high stringency i.e. greater than 98% identity over more than 95% of the total length of the query read and only uniquely matched reads were counted. The reference transcriptomes are described

in main text reference Lien et al. (2016) and have the SRA accession numbers as follows: Head kidney SRS639992, spleen SRS640021, gill SRS639861, heart SRS639994, liver SRS640002, nose SRS640009, gut

SRS639990, brain SRS639037, ovary SRS640030 and testis SRS640031. As nucleotide sequence identity between PSMA4.1a and PSMA4.1b was 100%, these RPKM values are merged. Cells are color coded

according to RPKM values where grey is negligible expression with RPKM values below 1, white is low expression levels with RPKM values 1-10, blue is medium levels 10-50, green is high expression levels

50-100 and orange is very high levels with RPKM values >100. Yellow colored cells show the down-regulated genes identified in resistant animals. Gene annotations in the Sally genome alongside our unique gene

identifiers are shown

Appendix C

5

Appendix C.4. Alignment of selected deduced PSMA7 and PSMA8 amino acid sequences

* 20 * 40 * 60 * 80

hsPSMA8 : MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERTVRKICALDDHVCMAFAGLTADARVV : 85

SasaPSMA8.1b : ..A....................................KD...............E..........................I. : 85

SasaPSMA8.1a : ..A....................................KD...............E..........................I. : 85

SasaPSMA8.2a : ..A....................................KD...............E....C...T.................I. : 85

SasaPSMA8.2b : ..A....................................KD...............E..........................I. : 85

EsluPSMA7.Like: ..A....................................KD...............E..........................I. : 85

EsluPSMA7(2) : ..A....................................KD...............E..........................I. : 85

DarePSMA8 : ..A....................................KD...............E...........E..............I. : 85

AsmePSMA8 : ..A....................................KD...............E...........E..............I. : 85

LeocPSMA8 : ..A....................................RD...............E...........E..............I. : 85

OrmePSMA8 : ..A....................................KD...............E...........E..............I. : 85

OrniPSMA8 : ..A....................................KD...............E...........E..............I. : 85

GaacPSMA8 : ..A....................................KD...............E...........E..............I. : 85

hsPSMA7 : --MS................................V..RD............................N.............I. : 83

SasaPSMA7a : --MS................................V..K.V........T......D...........N.F...........I. : 83

SasaPSMA7b : --MS.................D..............V..K..........T.....ED.......S...N.F...........II : 83

EsluPSMA7(1) : --MS................................V..KK..........I.....D...........N.FL............ : 83

LeocPSMA7 : --MS................................V..K...........M.....D...........N.F...........I. : 83

IcpuPSMA7 : --MS................................V..K...........A.M...............N.F...........I. : 83

AsmePSM7 : --MS................................V..K...I.......A.M...D...........N.F...........II : 83

ClhaPSMA7 : --MS.............................V..V..KT...V......L.....D.......P...N.F...........I. : 83

Appendix C

6

* 100 * 120 * 140 * 160 *

hsPSMA7.Like : INRARVECQSHKLTVEDPVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDDDGISRLYQTDPSGTYHAWKANAIGRSAKTV : 170

SasaPSMA8.1b : ...........R..............H......R....................N..TP.................S........ : 170

SasaPSMA8.1a : ...........R..............H......R....................C..TP.......................... : 170

SasaPSMA8.2a : ...........R..............H......R....................C..TP.......................... : 170

SasaPSMA8.2b : ...........R..............H......R....................Y..TP.......................... : 170

EsluPSMA7.Like: ...........R..............H......R....................Y..TP.......................... : 170

EsluPSMA7(2) : ...........R..............H......R....................Y..TP.......................... : 170

DarePSMA8 : ...........R..............Y......R....................Y..TP.......................... : 170

AsmePSMA8 : ...........R..............Y......R....................Y..TP.......................... : 170

LeocPSMA8 : ...........R..............Y......R....................Y..TP.......................... : 170

OrmePSMA8 : ...........R..............Y......R....................Y..TP.......................... : 170

OrniPSMA8 : ...........R..............Y......R....................Y..TP.......................... : 170

GaacPSMA8 : ...........R..............Y......R.......................TP.......................... : 170

hsPSMA7 : ...........R..............Y..S...R....................F..TP.....................G..S. : 168

SasaPSMA7a : V..........R................SSI..R.............S......F..TPH......................... : 168

SasaPSMA7b : V..........R......A.......Y.SSI..R.............S......F..TPH......................... : 168

EsluPSMA7(1) : V..........R................SSI..R.............S......F..TPH....E.................... : 168

LeocPSMA7 : ...........R..............Y.SS...R.............S......F..TP.....................A.... : 168

IcpuPSMA7 : ...........R......A.......Y.SNM..R.............S..M...F..TPN.......................S. : 168

AsmePSM7 : ...........R........I.....Y.SSM..R....................F..TPN..............VGD.------- : 161

ClhaPSMA7 : ...........R................CN...R.............S..A...F..TPH.....................G... : 168

Appendix C

7

180 * 200 * 220 * 240 *

hsPSMA7.Like : REFLEKNYTEDAIASDSEAIKLAIKALLEVVQSGGKNIELAIIRRNQPLKMFSAKEVELYVTEIEKEK-EEAEKKKSKKSV-- : 250

SasaPSMA8.1b : .........DE...T.N........................V........LLES..I.TL.A......E.......P...T-- : 251

SasaPSMA8.1a : ..........ES..T.N........................V.....S..LLES..I.TL........E.......Q...T-- : 251

SasaPSMA8.2a : .........DE...T.N.S......................V........LLES..I.TL...M....ED......Q...T-- : 251

SasaPSMA8.2b : ..........E...T.N.....................D..V........LLES..I.TL.A......ED......Q...T-- : 251

EsluPSMA7.Like: .........DE...T.ND....S..................V........LLES..I.TL.A......E.......Q...T-- : 251

EsluPSMA7(2) : .........DE...T.ND.......................V........LLES..I.TL.A......E.......Q...T-- : 251

DarePSMA8 : .........DE.....ND.......................V........ILES..I.TL.A......E.......Q...S-- : 251

AsmePSMA8 : .........DE.L...ND.......................V........ILES..I.TL.A......E.......Q..AS-- : 251

LeocPSMA8 : ..........E...T.ND.......................V........ILES..I.TL.A......E.......Q..AT-- : 251

OrmePSMA8 : .........DE...G.N........................V........ILES..I.TL.S......E.......Q...T-- : 251

OrniPSMA8 : ..........E...G.N........................V........ILES..I.TL........E.......Q...T-- : 251

GaacPSMA8 : .........DE...G.N........................V........ILES..I.TL.A......E.......Q...T-- : 251

hsPSMA7 : .........DE..ET.DLT...V..................VM..D.S..ILNPE.I.K..A......-..N....Q..AS-- : 248

SasaPSMA7a : ........K.EHME..TDT.....R.................MK.DESM.ILVQE.I.E...A.....E.AEKQ..KT----- : 246

SasaPSMA7b : ........K..-ME..TDT.....R.................MK.DESM.ILVQE.I.E...A..T..--AEKQ..P------ : 242

EsluPSMA7(1) : ......H.K.EDME..T.V.....R.................MKQDESM.ILDQE.IKK...A..N..E.AEK...T------ : 245

LeocPSMA7 : ........KDEDME..K.TV..V.R................VM..DE...ILDQE.I.K..S......E.TEKQ..KT----- : 246

IcpuPSMA7 : ........K.EDMDT.ELVL...VR................TMK.GEAM.ILDPE...KI.SAL....E.AEK...AAT.TTS : 251

AsmePSM7 : -----------------------------............TMKNGETM.ILEPA...NI.AA.....E.ADK...ATA.AST : 215

ClhaPSMA7 : ......S.K.EDVE..EG.......................VMKQ.EA..ILDPE..DKH.LAL....E.ADK...T------ : 245

Amino acid alignment of selected proteasome PSMA7 and PSMA8 (or 7-like) sequences used to produce phylogenetic tree shown in main text Figure 4. Numbering above the alignment refers

to mature human PSMA7.Like (alias hsPSMA8). Amino acids are colored according to physiochemical properties. Gaps shown with a dash are introduced to optimize the alignment using the

human sequence PSMA.Like sequence as a reference. Species are reflected in sequence names as follows: Leoc is Lepisosteus oculatus (Spotted gar), Sasa is Salmo salar (Atlantic salmon),

Eslu is Esox lucius (Northern pike), Asme is Astyanax mexicanus (Mexican tetra), Clha is Clupea harengus (Atlantic herring), Dare is Danio rerio (zebrafish), Icpu is Ictalurus punctatus

(catfish), Orme is Oryzias melastigma (marine medaka), Gaac is Gasterosteus aculeatus (stickleback) and Orni is Oreochromis niloticus (tilapia). Sequence references are shown in Appendix

C.5.

Appendix C

8

Appendix C.5. Deduced proteasome amino acid sequences used in phylogenies

>SasaPSMB6a ssa04 NC_027303.1:35.985.620-35.988.142 XP_014051784.1

MAAYMSASHSENAFSTHDFVPEWAQEEVSTGTTIMAVEFDGGVVIAADSRTTTGAYIANR

VTDKLTPIHDHIFCCRSGSAADTQAVADIVTYQLGFHSIELDEPPLVQTAANLFKQTCYR

YREELMAGIIVAGWDKRRGGQVYTVPMGGMIVRQPVSVGGSGSSYIYGFMDSNYKPGMTK

EECLHFCTQALALAMERDGSSGGVARLAAITEEGLERRVVLGNQLPRFSNA

>SasaPSMB6b ssa11 NC_027310.1: 77.336.949-77.339.681 XP_013984457.1

MATAYMSANRSENAFSTADLVPEWAQEEVSTGTTIMAVEFDGGVVIGADSRTTTGAYIAN

RVTDKLTPIHDRIFCCRSGSAADTQAVADIVTYQLGFHSIELDEPPLVQTAANLFKQTCY

RYREELMAGIIVAGWDKRRGGQVYTVPMGGMIVRQPVSVGGSGSSYIYGFMDSNYKPGMT

KEECLHFCTQALALAMERDGSSGGVARLAAITEEGLERRVVLGNQLPKFSNA

>SasaPSMA4.1a ssa26 NC_027325.1:32939806-32943155 NP_001134515.1

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGSCLGILANDGVLLAAERRNIHKLLDEVF

FSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQA

YTQFGGKRPFGVSLLYMGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDFK

EGEMSLSSALALAVKVLNKTMDVSKLSAEKVEIATLTREDGKTKIKVLKQKDVEELIKRH

EAEEAKAEKDKKDKEQKEKDK

>SasaPSMA4.1b ssa11 NC_027310.1:38380642-38383977 XP_013983107.1

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGSCLGILANDGVLLAAERRNIHKLLDEVF


YTQFGGKRPFGVSLLYMGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDFK

EGEMSLSSALALAVKVLNKTMDVSKLSAEKVEIATLTREDGKTKIKVLKQKEVEELIKRH

EAEEAKAEKDKKDKEQKEKD

>SasaPSMA4.2 ssa10 NC_027309.1:62947128-62979393 XP_013979513.1

MLFPQMELILKETTKMSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLL

AAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPI

PCEQLVTALCDIKQAYTQFGGKRPFGVSLLYMGWDKHYGFQLYQSDPSGNYGGWKATCIG

NNSAAAVSMLKQDFKEGEMSLSSALALAVKVLNKTMDVSKLSAEKVEIATLTRENGKTCI

KVLKQKEVDELIKKHEAEEAKAEKEKKEKEQKEKDK

>SasaPSMA6.1a ssa06 NC_027305.1:48395986-48398391 XP_014060163.1

MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVVVTQKKVPDKL

LDASTVTHLFRITENIGCVMSGMTADSKSQVQRARYEAANWKYKYGYEIPVDMLCKRIAD

ISQVYTQNAEMRPLGCCMIVIGVDEECGPQVYKCDPAGYYCGFKATAAGVKQTEATSFLE

KKVKKKLDWTFKETVETAITCLSTVLSIDFKPSELEIGVITTEDPKFRILTESEVDIHLV

SLAERD


MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVVVTQKKVPDTL

LDSSTVTHLFRITENIGCVMSGMTADGKSQVQRARYEAANWNYKYGYEIPVDMLCKRIAD

ISQVYTQNAEMRPLGCCMIVIGVDDECGPQVYKCDPAGYYCGFKATAAGVKQTEATSFLE

Appendix C

9

KKVKKKLDLTFKETVETAITCLSTVLSIDFKPSELEIGVISTEDPKFRILTESEVDTHLV

SLAERD


MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSLAIRGQDCAVVVTQKKVPDKL

LDASTVTHLFKITENIGCVMSGMTADSKSQVQRARYEAANWKYKYGYEIPVDMLCKRMAD

ISQVYTQNAEMRPLGCCMIVIGVDEECGPQVYKCDPAGYYCGFKATAAGVKQTEATTFLE

KKVKKKLDWTFAQTIETAITCLSTVLSIDFKPSELEIGVITTEDPKFRILTETEIDVHLV

SLSERD


MSRGSNAGFDRHITIFSPEGRLYQVEYAFKAIAQGGLTSLGVRGTDCAVVVTQKKVPDKL

LDSATLTNMYPLTPRIGCVMTGHNADSRSQVHRARVEAAEWKYKFGYDITADMLCRRMAD

ISQVYTQNAEMRPLGCCMILVAMDPQLGPMLYKCDPAGYFCGFRATSVGAKQTEANSYLE

KKLKKRPELSYEMTVELAISCLSSILSMDFKPSEIEVGVVTKESPKFRTLSETEIDTHLV

AIAERE

>SasaPSMA7a ssa15 NC_027314.1:60419652-60421283 XP_014000965.1

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGKNVVVLGVEKKTVAKLQDDRTVR

KICALDDNVFMAFAGLTADARIVVNRARVECQSHRLTVEDPVTVEYITRFISSIKQRYTQ

SNGRRPFGISSLIVGFDFDGTPHLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYKEEH

MESDTDTIKLAIRALLEVVQSGGKNIELAIMKRDESMKILVQEEIEEYVTAIEKEKEEAE

KQKKKT

>SasaPSMA7b ssa13 NC_027312.1:19630566-19634058 XP_013990556.1

MSYDRAITVFSPDGHLFQVDYAQEAVKKGSTAVGVRGKNIVVLGVEKKTVAKLQEDRTVR

KICSLDDNVFMAFAGLTADARIIVNRARVECQSHRLTVEDPATVEYITRYISSIKQRYTQ

SNGRRPFGISSLIVGFDFDGTPHLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYKEDM

ESDTDTIKLAIRALLEVVQSGGKNIELAIMKRDESMKILVQEEIEEYVTAIETEKAEKQK

KP


MAARYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGKDIVVLGVEKKSVAKLQEERT

VRKICALDDHVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRHIATLKQRY

TQSNGRRPFGISALIVGFDCDGTPRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTE

ESIATDNEAIKLAIKALLEVVQSGGKNIELAVIRRNQSLKLLESKEIETLVTEIEKEKEE

EAEKKKQKKST




TQSNGRRPFGISALIVGFDNDGTPRLYQTDPSGTYHAWKANSIGRSAKTVREFLEKNYTD

EAIATDNEAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKLLESKEIETLVAEIEKEKEE

EAEKKKPKKST



VCKICTLDDHVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRHIATLKQRY

Appendix C

10

TQSNGRRPFGISALIVGFDCDGTPRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTD

EAIATDNESIKLAIKALLEVVQSGGKNIELAVIRRNQPLKLLESKEIETLVTEMEKEKED

EAEKKKQKKST




TQSNGRRPFGISALIVGFDYDGTPRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTE

EAIATDNEAIKLAIKALLEVVQSGGKNIDLAVIRRNQPLKLLESKEIETLVAEIEKEKED

EAEKKKQKKST

>OrmePSMA8 [Oryzias melastigma] marine medaka TSA:GDGP01030614 Om_CDS_27313-1 transcribed RNA sequence


VRKICALDEHVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIATLKQRY

TQSNGRRPFGISALIVGFDYDGTPRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTD

EAIAGDNEAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKILESKEIETLVSEIEKEKEE

EAEKKKQKKST*

>OrniPSMA8 [Oreochromis niloticus] proteasome subunit alpha 8 ENSONIG00000009734 Scaffold GL831134.1: 5,204,529-5,208,745




EAIAGDNEAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKILESKEIETLVTEIEKEKEE

EAEKKKQKKST

>GaacPSMA8 [Gasterosteus aculeatus] proteasome subunit alpha 8 ENSGACT00000017525.1 groupIII: 948,765-953,424 251 bp



TQSNGRRPFGISALIVGFDDDGTPRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTD

EAIAGDNEAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKILESKEIETLVAEIEKEKEE

EAEKKKQKKST

>IcpuPSMA7 [Ictalurus punctatus] PREDICTED: proteasome subunit alpha type-7 XP_017343612.1

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGKNIVVLGVEKKSAAMLQDERTVR

KICALDDNVFMAFAGLTADARIVINRARVECQSHRLTVEDPATVEYITRYISNMKQRYTQ

SNGRRPFGISSLIMGFDFDGTPNLYQTDPSGTYHAWKANAIGRSAKSVREFLEKNYKEED

MDTDELVLKLAVRALLEVVQSGGKNIELATMKRGEAMKILDPEEVEKIVSALEKEKEEAE

KKKKAATSTTS

>EsluPSMA7 (1) [Esox Lucius] PREDICTED: proteasome subunit alpha type-7 XP_010894973.1 LG12:27743363-27748418

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGKKIVVLGVEKKSIAKLQDDRTVR

KICALDDNVFLAFAGLTADARVVVNRARVECQSHRLTVEDPVTVEYITRFISSIKQRYTQ

SNGRRPFGISSLIVGFDFDGTPHLYQTEPSGTYHAWKANAIGRSAKTVREFLEKHYKEED

MESDTEVIKLAIRALLEVVQSGGKNIELAIMKQDESMKILDQEEIKKYVTAIENEKEEAE

KKKKT

>EsluPSMA7 (2) [Esox Lucius] proteasome subunit alpha type-7 NP_001290983.1 LG08:24735569-24737952


Appendix C

11



EAIATDNDAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKLLESKEIETLVAEIEKEKEE

EAEKKKQKKST

>EsluPSMA7-Like [Esox Lucius] PREDICTED: proteasome subunit alpha type-7-like XP_010882855.1 LG03:9081749-9084178




EAIATDNDAIKLSIKALLEVVQSGGKNIELAVIRRNQPLKLLESKEIETLVAEIEKEKEE

EAEKKKQKKST

>DarePSMA8 [Danio rerio] proteasome subunit alpha 8 ENSDARG00000010965 Chr.20: 18,406,539-18,414,281




EAIASDNDAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKILESKEIETLVAEIEKEKEE

EAEKKKQKKSS

>AsmePSMA7 [Astyanax mexicanus] proteasome subunit alpha 7 XP_015457589.1 ENSAMXG00000002859 Scaffold KB871891.1: 375,519-

378,990

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGKNIVILGVEKKSAAMLQDDRTVR

KICALDDNVFMAFAGLTADARIIINRARVECQSHRLTVEDPVTIEYITRYISSMKQRYTQ

SNGRRPFGISALIVGFDFDGTPNLYQTDPSGTYHAWKVGDIVVQSGGKNIELATMKNGET

MKILEPAEVENIVAAIEKEKEEADKKKKATASAST

>AsmePSMA8 [Astyanax mexicanus] proteasome subunit alpha 8 ENSAMXG00000019432 Scaffold KB871746.1: 9,366-16,256




EALASDNDAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKILESKEIETLVAEIEKEKEE

EAEKKKQKKAS

>ClhaPSMA7 [Clupea harengus] PREDICTED: proteasome subunit alpha type-7 XP_012686185.1

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTVVGVRGKTIVVVGVEKKSLAKLQDDRTVR

KICPLDDNVFMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRFICNLKQRYTQ

SNGRRPFGISSLIAGFDFDGTPHLYQTDPSGTYHAWKANAIGRSGKTVREFLEKSYKEED

VESDEGAIKLAIKALLEVVQSGGKNIELAVMKQNEALKILDPEEVDKHVLALEKEKEEAD

KKKKT

>LeocPSMA7 [Lepisosteus oculates] proteasome subunit alpha 7 ENSLOCG00000003735 Chr.LG18: 7,557,365-7,561,553

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGKNIVVLGVEKKSMAKLQDDRTVR

KICALDDNVFMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYISSLKQRYTQ

SNGRRPFGISSLIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRAAKTVREFLEKNYKDED

MESDKETVKLVIRALLEVVQSGGKNIELAVMRRDEPLKILDQEEIEKYVSEIEKEKEETE

KQKKKT

>LeocPSMA8 [Lepisosteus oculates] proteasome subunit alpha 8 ENSLOCG00000006356 Chr.LG9: 12,636,489-12,646,112

Appendix C

12

MAARYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGRDIVVLGVEKKSVAKLQEERT



EAIATDNDAIKLAIKALLEVVQSGGKNIELAVIRRNQPLKILESKEIETLVAEIEKEKEE

EAEKKKQKKAT

>hsPSMA4 [Homo sapiens] NP_002780 proteasome subunit alpha type-4

MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVF


YTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYK

EGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKH

EEEEAKAEREKKEKEQKEKDK

>hsPSMA6 [Homo sapiens] NP_002782 proteasome subunit alpha type-6

MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKL

LDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIAD

ISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLE

KKVKKKFDWTFEQTVETAITCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLV

ALAERD

>hsPSMA7 [Homo sapiens] proteasome subunit alpha type-7 NP_002783 Chr.20: 62,136,735-62,143,430

MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVR

KICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQ

SNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEA

IETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENE

KKKQKKAS

>hsPSMA7_Like (alias PSMA8) [Homo sapiens] proteasome subunit alpha type-7-like NP_001020267.1 Chr.18: 26,133,877-26,193,355

MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERT

VRKICALDDHVCMAFAGLTADARVVINRARVECQSHKLTVEDPVTVEYITRFIATLKQKY

TQSNGRRPFGISALIVGFDDDGISRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTE

DAIASDSEAIKLAIKALLEVVQSGGKNIELAIIRRNQPLKMFSAKEVELYVTEIEKEKEE

AEKKKSKKSV

>hsPSMB6 [Homo sapiens] NP_002789 proteasome subunit beta type-6

MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYI

ANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEM

CYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREG

MTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA

appendix c - ars.els-cdn.com8: sspsma4.1b 51 50 50 50 50 49 100 100 85 46 46 46 46 38 37 9:...

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