appendix i composition of media used composition of...

177

177

Appendix I

COMPOSITION OF MEDIA USED

Composition of Nutrient broth

Ingredients g/L

Peptic digest of animal tissue 10.00

Sodium chloride 5.00

Beef extract 5.00

Distilled water 1 liter

pH 7.4 ± 0.2

Composition of Nutrient agar: 1.5 per cent agar added to Nutrient broth

Composition of Pikovskaya’s medium

Ingredients g/L

Glucose 10.0 g

Tricalcium phosphate 5.0 g

Ammonium sulphate 0.5 g

Potassium chloride 0.2 g

Magnesium sulphate 0.1 g

Manganese sulphate Trace

Ferrous sulphate Trace

Yeast extract 0.5 g

Agar 15.0 g

PH 7.0 ± 0.2

178

178

Composition of King’s B agar

Ingredients g/L

Proteose peptone No.3 20.0

Dipotassium hydrogen phosphate 1.50

Magnesium sulfate heptahydrate 1.50

Agar 20

pH 7.2 + 0.2

Composition of Jensen’s medium

Ingredients g/L

Sucrose 20.0


Magnesium sulphate 0.5

Sodium chloride 0.5

Ferrous sulphate 0.1

Sodium molybdate 0.005

Calcium carbonate 2.0

Distilled water 1 litre

Composition of Jensen’s agar medium: 1.5 per cent agar added to Jensen’s medium

Composition of NBRIP broth

Ingredients g/L

Glucose 10

Tricalcium phosphate 5

Magnesium chloride hexahydrate 5

Magnesium sulfate heptahydrate 0.25

Potassium chloride 0.2

Ammonium sulfate 0.1

Double distilled water 1.0

179

179

Composition of Peptone water

Ingredients g/L



Distilled water 1 litre

pH 7.5 ± 0.2

Composition of Potato Dextrose Agar (PDA)

Ingredients g/L

Potatoes infusion 200.0

Dextrose 20.0

Agar 15.0

pH 5.6 ± 0.2

Composition of Trypticase soya broth

Ingredients g/L

Pancreatic digest of casein 17.0

Papaic digest of soyabean meal 3.0

Sodium chloride 5.0


Dextrose 2.5

pH 7.3 ± 0.2

180

180

Composition of Succinate Broth

Ingredients g/L

Dipotassium Hydrogen orthophosphate 6.00

Potassium dihydrogen orthophosphate 3.00

Magnesium sulphate heptahydrate 0.2

Ammonium sulphate

Succinic acid

Distilled water

pH

1.0

4.0

1 litre

7.0

Composition of Trypticase Soya Agar

Ingredient g/L

Tryptone 17.00

Peptone 3.00


Glucose 2.50

Dipotassium phosphate

Agar

Distilled water

pH

2.50

20

1 litre

7.2±0.2

181

181

Compostion of Anaerobic agar (Brewer)

Ingredient g/L

Proteose peptone 10.000

Casein enzymic hydrolysate 5.0

Yeast extract 5.0

Dextrose 10.0

Sodium chloride 5.0

Sodium thioglycollate 2.0

Sodium formaldehyde sulphoxylate 1.0

Resazurin 0.002

Agar 15.0

pH 7.2±0.2

Composition of Actinomycetes Isolation Agar

Ingredient g/L

Sodium caseinate 2.0

L-Asparagine 0.1

Sodium propionate 4.0

Dipotassium phosphate 0.5



Agar 15.0

pH 8.1±0.2

182

182

Composition of Carboxy Methyl Cellulose (CMC) medium

Ingredient g/L

Carboxymethyl cellulose 2.0

Sodium nitrate 1.0

Dipotassium phosphate 1.0

Potassium chloride 1.0



Yeast extract 5.0

pH 7.2±0.2

Composition of Carboxy Methyl Cellulose (CMC) agar medium: 1.5 per cent agar

added to carboxymethyl cellulose (CMC) medium

Composition of Colloidal Chitin medium

Ingredient g/L

Colloidal chitin 5



Dihydrogen potassium phosphate 0.3


Mangenese chloride 0.001

Sodium chloride 0.3

Yeast extract 0.2

Agar 15.0

pH 7.0±0.2

Composition of Colloidal Chitin agar medium: 1.5 per cent agar added to colloidal

chitin medium

183

183

Composition of Tributyrin agar medium

Ingredient g/L


Yeast extract 3.0

Agar 15.0

pH 7.0±0.2

After dissolving the media in 990 mL of distilled water, 10 mL of tributyrin was added

Composition of Aleksandrov agar medium

Ingredient g/L

Glucose 10

Yeast extract 5


Ferric chloride 0.1

Calcium carbonate 0.1

Calcium phosphate 2

Potassium aluminium silicate 5

Agar 15.0

pH 7.0±0.2

All the above media were sterilized in autoclave at 121º C at 15lb/inch2 for 20 min

184

184

Appendix II

Reagents

1.) Barton’s reagent

For the preparation of Barton’s reagent following two solutions were prepared:

I. Solution A: 25 g ammonium molybdate was dissolved in 400 mL ddw.

II. Solution B: 1.25 g ammonium metavanadate was dissolved in 300 Ml of boiling

water, cooled and then 250 mL concentrated HNO3 was added to it.

Then solution A and B were mixed and volume made upto one litre.

2.) Chrome Azurol Sulphonate (CAS) reagent

CAS assay solution:

a) Solution A (2 mM CAS stock solution): 0.121 g of CAS was dissolved in 100

mL distilled water.

b) Solution B (1 mM Fe stock solution): 1 mM solution of FeCl3.6H2O was made

in 10 mM HCl.

c) Solution C (Piperazine buffer): 4.307 g Piperazine was dissolved in 30 mL

distilled water. The pH of the solution was adjusted to 5.6 by adding concentrated

HCl to the solution

d) Solution D (HDTMA solution): 0.0219 g HDTMA was dissolved in 50mL

distilled water.

1.5 mL of solution B was mixed with 7.5 mL of solution A and this solution was added to

solution D in a measuring cylinder. Solution C was then added to it and the final volume

was made up to 100mL with distilled water.

Shuttle solution: 0.2 M 5-sulfosalicylic acid solution was prepared and stored in the

darkness.

3.) Salkowski reagent: 1 mL of 0.5 M FeCl3 dissolved in 49 mL of 35% perchloric

acid.

185

185

4.) Nessler’s Reagent: 100 g Mercuric iodide and 70 g potassium iodide were

dissolved in a small quantity of distilled water and this mixture was added slowly,

with stirring, to a 500 mL cooled solution of sodium hyroxide (160 g sodium

hyroxide dissolved in 500 mL distilled water) and finally, this solution was diluted

to 1 litre with distilled water. Reagent was stored in rubber-stoppered borosilicate

glassware in dark to maintain its stability.

5.) DNS reagent: 2 g dinitrosalicyclic acid, 0.4 g phenol, 0.1 g sodium sulphite and 40

g Rochelle salt were dissolved in 100 mL of 2 % (w/v) NaOH solution and diluted

to 200 ml with distilled water

186

186

Appendix III

16S rRNA gene sequences of potential bacterial isolates submitted to

GenBank, National Center for Biotechnology Information (NCBI), USA

>CS4B1(KJ78117)

CTGGCACCAAAAATGTGCGGCCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGG

TAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAAC

ATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGG

CGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGC

AGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGC

TCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACT

ACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTT

CTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGA

GTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAA

CTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTA

AGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTG

AAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC

AGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCG

TCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGG

CACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGC

TACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTT

CGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTT

CCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACACCCTGAGCGAG

CCCGAGCCGGGGACCG

> CS4B2(KJ78118)

GACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACGGGGGCTAATACCGGATAACAT

TTTGAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGG

TGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCC

CAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATG

AAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTA

ACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCG

TAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAG

ACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGA

AGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCA

CGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTG

GGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA

AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTG

ACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTT

AGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCAT

CATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAAT

CTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCA

GCATGCCGCGGTGAATACGTTCCCGGGCCCTTTGTTACCTAACT

> CS4B3(KJ78119)

ACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATT

TTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGT

GAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCC

AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGA

AGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAA

CCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGT

AAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGA

CTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAA

GGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAC

GCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGG

GGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAA

GCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGA

CAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTA

GTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATC

ATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAATC

TCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAG

187

187

CATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGT

GGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATC

> CS4B4(KJ78120)

TGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGA

TGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATA

CCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCAT

TAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACT

GAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCG

CGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTT

GACCGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGA

ATTATTGGGCGTAAAGGGCTCGCAGGCGGTTCCTTAAGTCTGATGTGAAAGCCCCCGGATCAACCGGGGAGGGTCATTG

GAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAAC

ACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACC

CTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAG

CACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTG

GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCG

GGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAA

CCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGA

CGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCAAAACCGCGAG

GTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTA

ATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACA

CCCGAAGTCGGTGAGGTAACCTTTATGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGT

AGCCGTATCGGAAGGTGCGGCTGGATCA

> CS4B5(KJ78121)

CAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAA

CTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACT

TACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAG

GGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACG

AAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTAC

CGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACG

TAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCG

GCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAA

ATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGG

GGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTA

GTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCC

CGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCC

TAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGG

TTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACA

AACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAA

CAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTG

CGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGT

CACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAAATTATCTTCCAGG

> CS4B6(KJ78122)

GGGGCGTGCTATACATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACG

TGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTC

AAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACC

AAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGG

CAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTA

AAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCT

AACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCG

GTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAG

GAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCT

GTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT

GCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAG

ACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCT

TACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTT

GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGT

TGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCT

GGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTC

AGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATA

188

188

CGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGG

AGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAA

> CS3B5(KJ78123)

CGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGGAGTAACACGTGGGTAACCTGCCTGTAAGA

CTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCG

GCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCG

ACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCG

CAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAG

AACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGC

GGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGA

AAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGT

AGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCG

AAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTC

CGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTG

ACGGGGGCCCGCACCAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTC

TGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGA

GATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTG

CCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAA

TGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCA

ACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACAC

ACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGG

> CS3B6(KJ78124)

GGGGGGGGGGCCGCCGTTCTATACTGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGT

GAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAAC

CGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTA

ACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTC

CTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTT

TCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAA

AGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGG

CTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAG

TGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGAC

TCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTA

AACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTA

CGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACG

CGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTG

GTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCC

AGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCC

CTTATACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAA

TCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCG

CGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAA

CCTTTTAGGAGCCAGCCGCTGAAAGGGTGACCCGGGGA

> CS2B6(KJ78125)

TAATTTGGGTATCTCCAGCGGACAGAAGGGCTGGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACC

TGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATGGTTCAAGGATGAA

AGACGGTTTCGGCTGTCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACG

ATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAG

GGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGT

TGTTAGGGAAGAACAAGTGCAAGAGTAACTGCTTGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGC

CAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAG

TCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAA

TTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACG

CTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT

AGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTC

AAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCT

TGACATCCTCTGACAACCCTAGAGATAGGGCTTTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCT

CGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCT

AAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACA

CGTGCTACAATGGACAGAACAAAGGGCTGCGAGACCGCAAGGTTTAGCCAATCCCACAAATCTGTTCTCAGTTCGGATC

GCAGTCTGCAACTCGACTGCGTGAAGCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGC

CTTGTACACACCGCCCGTCACACCACGAGAGTTTTTCTATTTTATTTCCGT

189

189

> CS4A1(KJ78126)

GAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGG

AAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGAT

GGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATC

GGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCT

GACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGA

ATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGG

CAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCCGCTCAAC

CGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTA

GAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGA

ACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGC

AGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAA

GCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGAT

AGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC

CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGA

GGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGG

CAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAG

CTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCA

CGAGAGTTTGTAACACCCGAAGTCGGT

> CS4A2(KJ78127)

TTATTGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTGAG

AGCTTGCTCTCAAGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGG

AAACCGGGGCTAATACCGGATAAYATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGAT

GGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATC

GGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCT

GACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGA

ATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGG

CAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAAC

CGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTA

GAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAA

ACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGA

AGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAA

GCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGAT

AGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC

CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGA

GGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAG

CTGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGTAG

CTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCA

CGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTG

AAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCT

> CS4A3(KJ78128)

CAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTGAGAGCTTGCTCTCAAGAAGTTAGCGG

CGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAAT

ATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTT

GGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGG

CCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGA

TGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACC

TAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGG

CGTAAAGCGCGCGCAGGTGGTTTCTTAACTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGG

AGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGTAATGCGTAGAGATATGGAGGAACACCAGTGGC

GAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTC

CACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCC

TGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTC

GAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAG

TGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATC

TTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATC

ATCATGCCCCTTATGACCTGGGCTACATACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTA

ATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGAT

CAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTC

GGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATC

GGAAGGTGCGGCTGT

190

190

> CS4A4(KJ78129)

CAAGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGC

TAATACCGGATAACATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGT

CGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTG

GGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAA

CGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGC

ACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTAT

CCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGT

CATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGA

GGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAG

ATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCA

TTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGC

ATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTC

CTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAG

CGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGG

GATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACC

GCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGC

TAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTG

TAACACCCGAAGTCGGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAAGTCGTAAC

AAGGTAGCCGTATC

191

191

Appendix IV

Table 1: Percent inhibition in the growth of F. oxysporum by different concentration of cell-free

extracts of bacterial supernatants

Isolates Per cent inhibition in the growth of F. oxysporum by different

concentrations (v/v) of bacterial supernatants

100% 75% 50%

CS4B1 71 42 17.23

CS4B2 64.33 32.33 12.23

CS4B3 54.2 31.67 12

CS4B4 51.1 24.22 0

CS4B5 32.33 0 0

CS4B6 43.67 13.33 0

CS3B5 69 27.67 9.56

CS3B6 20.67 0 0

MTCC 7612 69.22 35 13

Table 2: Percent inhibition in the growth of S.rolfsii by different concentrations of cell-free


Isolates Per cent inhibition in the growth of S.rolfsii by different

concentrations of bacterial supernatants

100% 75% 50%

CS4B1 70.67 54.23 20.67

CS4B2 59.33 37.22 19.87

CS4B3 62.41 25.23 9

CS4B4 33.74 15.6 0

CS4B5 38.67 12.3 0

CS4B6 28.11 11 0

CS3B5 53.22 25.67 11

CS3B6 25.67 7.33 0

MTCC 7612 64 44 12

Table 3: Percent inhibition in the growth of P.aphanidermatum by different concentrations of cell-free extracts of bacterial supernatants

Isolates Per cent inhibition in the growth of P.aphanidermatum by different


100% 75% 50% 25%

CS4B1 70.67 54.23 20.67 80.23

CS4B2 59.33 37.22 19.87 83.67

CS4B3 62.41 25.23 9 82.29

CS4B4 33.74 15.6 0 68.67

CS4B5 38.67 12.3 0 62.21

CS4B6 28.11 11 0 65.04

CS3B5 53.22 25.67 11 80.22

CS3B6 25.67 7.33 0 68.66

MTCC 7612 64 44 12 81

192

192

Table 4: Percent inhibition in the growth of C.truncatum by different concentrations of cell-free


Isolates Per cent inhibition in the growth of C.truncatum by different


100% 75% 50%

CS4B1 71.3 43.2 16.62

CS4B2 68.33 28.65 6.72

CS4B3 69.67 30.33 0

CS4B4 54.33 26 0

CS4B5 24.11 0 0

CS4B6 30.26 17 0

CS3B5 63.57 38.67 11.67

CS3B6 46.33 15 0

MTCC 7612 67 37 9

Table 5: Percent inhibition in the growth of R.solani by different concentrations of cell-free extracts of bacterial supernatants

Isolates Per cent inhibition in the growth of R.solani by different


100% 75%

CS4B1 50.23 24.23

CS4B2 40.35 16.67

CS4B3 38.62 12.67

CS4B4 38.66 10.33

CS4B5 29.12 0

CS4B6 26 0

CS3B5 47.33 20.22

CS3B6 21.67 0

MTCC 7612 41 14

Table 6: Percent inhibition in the growth of A.solani by different concentrations of cell-free


Isolates Per cent inhibition in the growth of A.solani by different


100% 75%

CS4B1 39.33 16.33

CS4B2 29.67 13.23

CS4B3 20.33 0

CS4B4 24.67 0

CS4B5 11.33 0

CS4B6 18.33 0

CS3B5 31.23 11.67

CS3B6 6 0

MTCC 7612 30 0

193

193

Table 7: Percent inhibition by volatile metabolites in the growth of F. oxysporum at different

stages of bacterial growth

Isolates Percent inhibition in the growth of F. oxysporum at different stages of

bacterial growth

0h 24h 48h 72h

CS4B1 56.52 68.37 65.78 30.96

CS4B2 46.52 64.3 57.63 15.78

CS4B3 48.74 63.19 54.3 13.34

CS4B4 42.81 61.33 54.63 26.33

CS4B5 46.52 55.41 51.7 0

CS4B6 52.07 64.67 58 0

CS3B5 50.59 65.41 59.11 15.41

CS3B6 31.33 47.63 34.59 0

MTCC 7612 48 66 63 27

Table 8: Percent inhibition by volatile metabolites in the growth of S.rolfsii at different stages of

bacterial growth

Isolates Percent inhibition in the growth of S.rolfsii at different stages of bacterial

growth

0h 24h 48h 72h

CS4B1 51.85 68.15 61.11 43.7

CS4B2 47.78 62.96 54.81 30.37

CS4B3 49.26 58.15 55.56 21

CS4B4 44.81 56.3 55.19 11.45

CS4B5 37.41 50 37.78 0

CS4B6 32.96 55.19 40.37 20.65

CS3B5 51.11 61.85 59.63 23.7

CS3B6 8.15 33.33 21.48 0

MTCC 7612 47 60 51 16

Table 9: Percent inhibition by volatile metabolites in the growth of P.aphanidermatum at different stages of bacterial growth

Isolates Percent inhibition the growth of P.aphanidermatum at different stages of

bacterial growth

0h 24h 48h 72h

CS4B1 70.96 73.7 72.59 44.44

CS4B2 67.41 70.37 67.04 31.85

CS4B3 54.44 67.41 62.22 37.04

CS4B4 57.41 71.85 71.85 26.67

CS4B5 55.56 70.74 71.48 36.3

CS4B6 61.48 71.85 71.11 38.89

CS3B5 54.07 68.15 67.04 47.41

CS3B6 51.48 65.93 63.33 18.65

MTCC 7612 68 71 65 31

194

194

Table 10: Percent inhibition by volatile metabolites in the growth of C.truncatum at different

stages of bacterial growth

Isolates Percent inhibition in the growth of C.truncatum at different stages of

bacterial growth

0h 24h 48h 72h

CS4B1 43.75 68.33 55 25

CS4B2 43.75 62.08 50 19.67

CS4B3 43.75 63.33 44.17 10.33

CS4B4 36.25 58.75 41.25 16.56

CS4B5 30.83 55 37.08 0

CS4B6 45.42 64.17 50 0

CS3B5 46.67 61.25 52.92 17.08

CS3B6 14.58 37.08 20.83 0

MTCC 7612 45 65 41 14

Table 11: Percent inhibition by volatile metabolites in the growth of R. solani at different stages

of bacterial growth

Isolates Percent inhibition in the growth of R. solani at different stages of

bacterial growth

0h 24h 48h 72h

CS4B1 43.7 58.15 48.89 23.35

CS4B2 37.78 54.81 46.67 21.67

CS4B3 39.63 57.41 50.74 25.74

CS4B4 42.59 48.89 45.93 0

CS4B5 32.22 45.93 37.78 0

CS4B6 28.89 52.96 41.11 0

CS3B5 46.3 56.67 51.85 0

CS3B6 15.56 34.44 22.59 0

MTCC 7612 40 54 42 17

Table 12: Percent inhibition by volatile metabolites in the growth of A. solani at different stages

of bacterial growth

Isolates Percent inhibition in the growth of A. solani at different stages of

bacterial growth

0h 24h 48h 72h

CS4B1 28.63 39.61 33.33 0

CS4B2 22.35 35.69 30.2 0

CS4B3 28.24 35.69 32.16 0

CS4B4 21.96 27.45 20.78 0

CS4B5 18.82 34.12 18.43 0

CS4B6 26.27 36.86 31.37 0

CS3B5 25.88 40.78 35.29 0

CS3B6 5 13.73 1.57 0

MTCC 7612 11 31 28 0

appendix i composition of media used composition of...

Documents