Application Courses Welcome to the Customer Experience Centre in Glasgow

3Thermo Fisher Scientific | Customer Experience Centre in Glasgow2 Thermo Fisher Scientific | Customer Experience Centre in Glasgow

Welcome 3

Your contacts 5

Your registration – service and process 7

Location, transport, hotels 9

Things to do in Glasgow 11

The courses 13

Contents Welcome Testimonials

Welcome to our Customer Experience Centre in Glasgow.

We believe training and instruction to be a collaborative effort, one that leverages the best elements of learning and teaching. To that end, we have developed option- rich training programmes that are designed to exceed expectations and promote excellence. We invite you to explore the wealth of training options we offer to help you advance your science. Our specialists provide you with comprehensive training solutions that include:

•Bright and modern training facilities

•Face-to-face training

•Small classes

•Courses tailored to your needs

•Theory combined with practical sessions

•Highly qualified team of training experts

•Networking with other scientists

An overview of the training courses we offer can be found on pages 13-15.

All courses are offered in English. Should you prefer to take the course in German, Italian, French, Russian or Spanish then please speak to your training coordinator. They will be able to advise you on our other facilities which can accommodate your language needs.

Below you will find comments from some of your peers who have already attended one of our courses:

“A well-prepared training, which is concise and instructive.”

“It was very helpful for me to get started with iPSC cell culture.”

“Very effective, really enjoyed using the microscope systems with the screen displays.”

“Intensive course and concentrated study due to small training classes.”

“Have obtained an overview of the whole workflowand are now more confident to start working with theapplication in the lab at home.”

“Saved me weeks of trial and error.”

“Interesting and diverting presentations of practitioners. Comprehensive training documentation.”

“Interesting and diverting presentations of practitioners. Comprehensive training documentation.”

5Thermo Fisher Scientific | Customer Experience Centre in Glasgow4 Thermo Fisher Scientific | Customer Experience Centre in Glasgow

Your contacts

We are happy to answer any questions you may have relating to the customer training programmes via telephone or in person at our training centre.

Customer Experience Centre: Thermo Fisher Scientific Fleming Building3 Fountain DriveInchinnan Business ParkPaisley PA4 9RF Scotland

Phone: +44 (0)141 814 6100

E-mail: learn-europe@lifetech.com

Web: https://learn.lifetechnologies.com/europe/

If you have any queries concerning our training courses, please contact your training coordinator. We will happily offer you competent and personal advice or connect you with one of our experienced scientists to help you choose a course that fits your needs.

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1. Registration If you have decided to participate in one of our training courses, registration is easy and straightforward. You can register online at: https://learn.lifetechnologies.com/europe/

Your registration will be handled promptly and your training coordinator will be happy to help you with any questions.

2. ConfirmationOnce you have submitted your registration you will receive an automatic registration receipt. Your training coordinator will contact you shortly and send you a written confirmation via email with all relevant details.

To make your travel to and stay at our training centre as convenient as possible, you will receive a document with detailed travel information and a map as well as some tips on local accommodations*.

3. Visa We appreciate that ever changing passport and visa requirements can add an element of frustration to booking your training course. That’s why we are happy to help arrange your visa. Please contact your training coordinator for assistance with application procedures and forms.

As a rule, authorities require between two and ten working days to decide on an application for a short stay visa. During the peak travel season however there may be a waiting period for making an application to a UK authority. Persons requiring a visa to enter the UK should therefore submit their applications in good time.

4. Certification After the successful completion of the training course you will receive a certificate of training from your trainer.

5. Post training To get started and as a thank you for attending our courses, we’d like to help support your research with promotions especially for you. Please feel free to contact us after your training with any further questions, comments or proposals.

Your registration – service and process

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Transport options

By trainGlasgow Central is a major mainline terminus located in the city centre. Services include connecting links across Glasgow and surrounding areas (ScotRail) as well as connection to the South and England (Virgin trains).

By carGlasgow is well connected to the surrounding areas by a number of major roads, including motorways M8 (Glasgow - Edinburgh), M77/A77 (Prestwick Airport), M74 (South – Carlisle), M80 (North).

By planeIt takes around 15 minutes by car (9.5 miles) to reach the city centre from Glasgow Airport using M8 motorway. Edinburgh Airport is around 60 minutes by car (39 miles) using the M8 motorway.

A document with detailed travel information will be supplied with the email confirming your registration.

Location, transport, hotels

Hotel recommendations

Please see below a list of hotels and rates. You will receive detailed information with your registration confirmation.

* Please note the prices quoted are for information only and we recommend that you contact the hotels directly for current price information.

Hotel Website Room rates from*

Approximate distance from training centre

Holiday Inn (Glasgow Airport)

http://www.higlasgowairporthotel.co.uk £70 3 miles

Ashtree House Hotel http://www.ashtreehousehotel.com £80 5 miles

Glynhill Hotel http://www.glynhill.com £85 5 miles

Mar Hall Spa and Golf resort

http://www.marhall.com £116 5 miles

Radisson Blu (Glasgow City Centre)

http://www.radissonblu.co.uk/ hotel-glasgow

£119 10 miles

Hotel Indigo (Glasgow City Centre)

http://www.hotelindigoglasgow.com/ £80 10 miles

Aberdeen

Perth

Dundee

St Andrews

Edinburgh

Dumfries

Ayr

SCOTLAND

Carlisle

ENGLANDBelfast

NORTHERNIRELAND

Inverness

Glasgow

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Glasgow is the largest city in Scotland and the third largest in the United Kingdom with just over 1.1M inhabitants in the Greater Glasgow area. During your stay, if you prefer to explore the city, you can enjoy the cultural offerings of museums, art galleries and universities as well as an exciting array of shopping and eating experiences. For nature and sight-seeing lovers, Glasgow is surrounded by many beautiful lochs, islands, castles and country parks.

There are many cultural and historic attractions in the city centre including Glasgow Cathedral and the Glasgow School of Art as well as the vibrant atmosphere of the busy shopping area of Buchannan Street. In the west end of Glasgow, you will find the Kelvingrove Art Gallery and Museum, the Hunterian Museum and the University of Glasgow established in the 15th century.

Glasgow is also well-known for its original pubs. You should not miss Òran Mór in the west end.

Why not stay over the weekend after you have finished your application course? Whether you are looking for sightseeing, culture, education, leisure, shopping, or sport – discover Glasgow.

Away from the city centre of Glasgow, you can learn more about whisky production at the Auchentoshan Distillery which is only 8 miles outside the city and offers daily tours.

Scotland is famous for its islands. The isle of Arran, off the west coast, is known as “Scotland in miniature” and deserves a visit for the beautiful landscapes, pretty villages and Brodick castle.

Explore Loch Lomond and the Trossachs National Park or Stirling Castle all of which are accessible by car or can be easily organised through a tourism agency.

Edinburgh, Scotland's capital city is just one hour from Glasgow by car or train and it is well worth visiting. The Edinburgh castle is a big attraction and offers wonderful views of the city and surrounding areas.

This is your journey—we are here to facilitate and personalise the experience to ensure that it lives up to your expectations.

Enjoy your stay.

Things to do in Glasgow and the surrounding region

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The coursesIncrease your working knowledge of applications and instrument operation, including hands-on lab experience, with our Application Training Courses, available at our Customer Training Centre in Glasgow. Our centre is a state-of-the-art installation for training and collaborative research. We offer interactive instructor led training, a fully staffed working laboratory, small training classes and the unique opportunity to collaborate with our application scientists. Spend some time in our lab to get the most out of the time you spend in yours. We offer hands-on and practical training for a wide range of applications and all experience levels:

•Basic introductions to sequence analysis and real-time PCR applications

•Advanced applications such as forensics, pathogen detection, whole-genome analysis and next generation sequencing

•Hands-on stem cell training in techniques for culturing and characterising human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), as well as reprogramming techniques for the creation of iPSCs

Our classes are designed to help empower your research. The benefits of attending one of our courses include:

•Learn complete workflows

•Access the latest instrumentation and applications

•Interact with other researchers working on similar projects

Courses are delivered by our award-winning team of application scientists, who have extensive collective experience in using, training on and troubleshooting the entire portfolio. Their expertise plus our world-class facilities make us the ideal choice for all of your training needs.

Which course will be the right one for you?

What is the difference between Fast and Standard cycling conditions and what are their advantages? How do I use, maintain and optimise the use of my instrument? How can I setup an experiment on the instrument? How can I export my data? How do I set CT and baseline? What information can be retrieved from the multicomponent plot? What is ROXTM dye good for?

This course might be just right for you:• Real-timePCRapplications– Real-time (qPCR) instrument training (1 day)— Real-time PCR workflow (chemistries, data analysis, troubleshooting, instrument maintenance)

Which TaqMan™ assay should I use? Which gives best coverage? Which sequence criteria allow for good primer and probe design? How can I design my own custom assay and what tools can I use? Which SNPs assay should I use and why? How do I show CNVs or do population or association studies? What is my allele frequency and how do I read cluster plots?

These are some of the questions answered in this course:• Real-timePCRapplications-TaqMangenotyping workflow and data analysis (1 day)— Genotyping workflow and software use

How do I calculate dCT, ddCT? Which endogenous control do I use? How do I estimate which is best? What is a housekeeping gene? How do I estimate my qPCR efficiency and why is it so important? How do I validate a gene expression microarray?

This course will have the answers:• Real-timePCRapplications- Gene expression workflow and data analysis (1 day)— Gene expression workflow

Of course, we also offer a combination of these courses and you can book and participate in all modules during one 3 day session if you wish.

qPCR courses

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Sequencing coursesWhich procedures should I be performing regularly to maintain my sequencer? Why are the spectral and spatial calibrations needed? Which polymers can I use on my sequencer? What is the difference betweenfragment analysis and sequencing? How many colours are included in the DS-33 dye set?

This training will suit you:• IntroductoryDNAsequencingandfragmentanalysis training (3 days)— 3130/3130xl or 3500/ 3500xl Genetic Analyzers workflow for sequencing and fragment analysis

What is the difference between Sanger and Next Generation Sequencing (NGS)? Which software should I use to analyse my data? How can I understand ifthe sequencing run was successful and produced enough data for my purpose? What is the principle of Ion Torrent™ technology? What are the steps required to sequence with Ion Proton™ technology? Whichapplications are suitable for the Ion Proton compared to the Ion PGM™ Sequencer?

Then these courses will address your needs:• IonPGMsystemworkflowtraining(3days)— System workflow and basic data analysis— Introduction to Ion AmpliSeq™ library construction•IonProtonsystemworkflowtraining(3days)— System workflow and basic data analysis— Introduction to fragment library construction•IonPGMschoolforforensicapplicationsworkshop (1 day)— Ion PGM Sequencer workflow and hands-on sessions•BioInformaticsformolecularbiologists(5days)— Introduction to Perl programming, relational databases e.g. Linux, practical project

Which questions can I address with whole genome sequencing? How to prepare the library for whole genome sequencing? What are the quality/quantity requirements for the input gDNA when looking at whole genome sample preparation? What do I need the barcodes for?

This course is for you:• IonDNAlibraryprep&essentials(1day)— Working with DNA and prepare fragment genomic libraries for the Ion PGM and Proton workflow — Perform and interpret application essential quality control steps

Which questions can I address with Ion AmpliSeq technology compared to whole genome sequencing? How to choose or design the correct Ion AmpliSeq panel for my research? How to prepare the library for Ion AmpliSeq technology? What are the quality/quantity requirements for the input gDNA when looking at Ion AmpliSeq library preparation? What do I need the barcodes for? How can I ensure my library is good todo the next step in the Ion Torrent workflow for sphere preparation with the Ion OneTouch™ 2 or Ion Chef™ systems?

This course is for you:• IonAmpliSeqlibrarypreparationtraining(1day)— Ion AmpliSeq library construction & Custom Design for the Ion PGM and Proton workflow — Perform and interpret application essential quality control steps

Cell culture coursesI am just new into culturing stem cells and not sure what media to use. What is the difference between cells on feeder and feeder free? How can I characterise my cells to be sure they are pluripotent? How does reprogramming work?

This course is for you:• Stemcellworkshop(3days)— Culturing PSCs under feeder-dependent and feeder- free conditions — Reprogramming and identification of iPSCs — Differentiation and characterisation methods for PSCs

The courses (continued)

Find out more at learn.lifetechnologies.com/europe

The Application Training Courses and the products referred to in this brochure are For Research Use Only. Not for use in diagnostic procedures. © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. CO37025 0515

*Travel, accommodation and other incidental expenses relating to the meeting are the responsibility of the attendees.

Are you unsure which course will fully meet your needs? Please contact our training coordinators who will connect you with one of our scientists to determine which course will best meet your needs. Still not sure they’re right for you? We also offer consultation hours.

The courses mentioned in this brochure are just a small selection of the options available. If you would like more information on our complete training course offerings and to register then please contact our training course coordinators or visit our website at: learn.lifetechnologies.com/europe

Email the training team at: learn-europe@lifetech.com