Mean sampling point flows (m3/day)

Sampling dates ●1 ▲2 ■3 ●4 ▲5 ●6 ▲7 ■8 ●9

29-Apr-13 3,13E+07 4,80E+05 8,38E+06 3,97E+07 2,36E+05 3,99E+07 1,40E+06 8,47E+06 4,68E+07

10-Jun-13 4,02E+07 6,05E+05 1,16E+07 5,18E+07 2,35E+05 5,20E+07 1,44E+06 8,99E+06 6,25E+07

24-Jun-13 2,50E+07 5,24E+05 1,27E+07 3,77E+07 2,39E+05 3,79E+07 1,46E+06 1,27E+07 5,31E+07

22-Jul-13 1,10E+07 4,04E+05 5,44E+06 1,64E+07 2,36E+05 1,67E+07 1,27E+06 4,75E+06 2,22E+07

29-Jul-13 1,24E+07 4,67E+05 6,65E+06 1,91E+07 2,37E+05 1,93E+07 1,71E+06 7,08E+06 2,87E+07

5-Aug-13 9,85E+06 3,59E+05 5,01E+06 1,49E+07 2,06E+05 1,51E+07 1,05E+06 3,63E+06 2,12E+07

19-Aug-13 9,50E+06 3,40E+05 4,75E+06 1,43E+07 1,93E+05 1,44E+07 1,05E+06 3,89E+06 2,11E+07

2-Sep-13 9,42E+06 3,94E+05 5,10E+06 1,45E+07 1,96E+05 1,47E+07 1,19E+06 3,46E+06 1,86E+07

16-Sep-13 1,13E+07 3,72E+05 7,60E+06 1,89E+07 1,94E+05 1,91E+07 1,97E+06 3,63E+06 2,51E+07

30-Sep-13 1,25E+07 2,64E+05 7,69E+06 2,02E+07 2,39E+05 2,05E+07 1,67E+06 3,80E+06 2,79E+07

14-Oct-13 2,74E+07 3,52E+05 1,18E+07 3,92E+07 1,82E+05 3,94E+07 1,34E+06 5,36E+06 4,71E+07

28-Oct-13 3,62E+07 6,08E+05 1,85E+07 5,47E+07 2,94E+05 5,50E+07 1,52E+06 9,59E+06 6,40E+07

18-Nov-13 4,70E+07 5,15E+05 2,98E+07 7,68E+07 3,21E+05 7,71E+07 1,46E+06 1,58E+07 9,37E+07

2-Dec-13 3,48E+07 4,87E+05 2,11E+07 5,59E+07 3,29E+05 5,62E+07 1,38E+06 8,90E+06 6,39E+07

16-Dec-13 2,54E+07 4,60E+05 1,10E+07 3,64E+07 3,37E+05 3,67E+07 1,34E+06 7,26E+06 4,46E+07

6-Jan-14 3,58E+07 5,56E+05 1,68E+07 5,26E+07 2,34E+05 5,29E+07 1,44E+06 1,94E+07 7,12E+07

27-Jan-14 4,08E+07 7,18E+05 1,67E+07 5,75E+07 2,33E+05 5,77E+07 1,62E+06 1,80E+07 7,72E+07

3-Feb-14 4,19E+07 6,08E+05 2,10E+07 6,29E+07 2,39E+05 6,31E+07 1,61E+06 1,99E+07 8,59E+07

17-Feb-14 4,67E+07 5,63E+05 1,94E+07 6,62E+07 2,30E+05 6,64E+07 1,50E+06 2,02E+07 8,75E+07

3-Mar-14 4,92E+07 7,37E+05 2,00E+07 6,92E+07 2,34E+05 6,94E+07 2,37E+06 1,97E+07 8,88E+07

17-Mar-14 2,41E+07 5,23E+05 9,68E+06 3,38E+07 2,32E+05 3,40E+07 1,40E+06 1,27E+07 4,87E+07

30-Mar-14 1,75E+07 4,00E+05 7,34E+06 2,48E+07 2,39E+05 2,50E+07 1,42E+06 1,04E+07 3,72E+07

14-Apr-14 1,30E+07 3,97E+05 5,53E+06 1,85E+07 2,36E+05 1,87E+07 1,40E+06 8,47E+06 3,00E+07

28-Apr-14 1,25E+07 5,00E+05 4,75E+06 1,73E+07 1,98E+05 1,75E+07 1,33E+06 7,17E+06 2,71E+07

12-May-14 1,12E+07 4,94E+05 5,10E+06 1,63E+07 2,45E+05 1,66E+07 2,04E+06 8,29E+06 2,68E+07

Mean annual flow (m3/day) 2,54E+07 4,85E+05 1,17E+07 3,72E+07 2,40E+05 3,74E+07 1,49E+06 1,01E+07 4,88E+07

Table S 1: Mean measured flow in m3/day for each sampling points and times. ● (the river

Seine), ■ (Tributaries of the river Seine, the river Marne and the river Oise) and▲ (WWTP

effluents) represent the sampling points of the figure 1.

|Ct blank - Ct water sample|

Sampling

periods

Number of water

samples

% Recovery of global control

Extraction and reverse transcriptase control

Competitive inhibition control

median (min - max) median (min - max) median (min - max)

Summer 77 28 (21 - 33) 0.64 (0.11 - 1.46) 0.55 (0.09 - 1.27)

Autumn 66 23 (19 - 38) 0.48 (0.08 - 1.23) 0.43 (-0.13 - 1.06)

Winter 66 25 (18 - 34) 0.57 (0.22 - 1.39) 0.62 (0.04 - 1.33)

Spring 66 25 (18 - 31) 0.61 (0.28 - 1.29) 0.48 (0.18 - 1.58)

Table S 2: Monitoring of inhibition related to water samples on obtained results and % recovery of global control during the whole sampling campaign (n = 275).

Figure S 1: Correlation between observed viral loads and number of fecal indicators (E. coli and intestinal enterococcus ) in surface water sample (n = 150) during the whole sampling campaign. For AdV, Spearman correlation coefficient (r) and p-value were 0,01042 (-0,1751 to 0,1548) and 0,8993 with E. coli and 0,003575 (-0,1707 to 0,1637) and 0,9658 with intestinal enterococcus. For NVGI, r and p-value were 0,07586 (-0,09023 to 0,2378) and 0,3562 with E. coli and 0,08325 (-0,08514 to 0,2470) and 0,3178 with intestinal enterococcus, respectively. For NVGII, r and p-value were -0,03535 (-0,1991to 0,1304) and 0,6676 with E. coli and -0,02022 (-0,1868 to 0,1475) and 0,8086 with intestinal enterococcus, respectively. For RV-A, r and p-value were -0,1348 (-0,2932 to 0,03087) and 0,1001 with E. coli and -0,09028 (-0,2537 to 0,07810) and 0,2785 with intestinal enterococcus, respectively. (-) means not detected.