Training program in Assisted Reproductive Technology (ART)Since its inception in 2003, our ART training program offers a unique opportunity to learn the latest techniques in this rapidly changing subspecialty at one of the world’s premier ART centers:

• World-renowned faculty• State of the art training facilities• Personalized hands-on training• Multiple comprehensive training modules• Excellent trainee satisfaction rates• Trained over 209 alumni from 45 countries since 2003• Certificate of Training awarded at the completion of training

This intensive, 320-hour course is available exclusively to scientists and physicians with a specific interest in reproductive endocrinology, embryology or andrology as well as other health professionals such as embryologists, reproductive biologists and technologists.

The course covers: • In vitro fertilization (IVF) • Intracytoplasmic sperm injection (ICSI) • Embryo biopsy • Vitrification • Gamete cryopreservation • Routine and advanced tests in andrology

Referral to a Clinical IVF Lab may be available to select candidates looking for clinical observation in the following areas:

• Logistics of running an IVF Lab • Observe IVF/ICSI procedures • Oocyte retrieval • Oocyte assessment • Fertilization check • Embryo assessment • Intrauterine insemination

Learn from the LeadersThe American Center for Reproductive MedicineCleveland Clinic’s American Center for Reproductive Medicine was established 27 years ago in 1993 with a three-fold mission: to conduct cutting-edge research into the causes of human reproductive pathologies, develop new fertility biomarkers and treatment modalities, and train physicians and scientists with a commitment to advancing the understanding of human reproduction, infertility, and fertility preservation.

Course ContentHands on Assisted Reproduction (IVF/ICSI) - Module 5A - 5C (160 hours)Learn basic and advanced techniques in:IVF Techniques• Module 5A • ICSI: Intracytoplasmic sperm injection • Classical IVF • Oocyte handling and grading • Embryo handling and grading • Catheter loading for embryo transfer

• Module 5B • Preparation of ICSI dishes • ICSI: injection of the spermatozoon into the oocyte • Oocyte handling (using fresh mouse oocytes)

Laboratory Training• Equipments • Micromanipulators: components and mechanics • Installing and aligning ICSI instruments

• Supplies & Culture Medium • Preparation of culture medium and dishes for IVF/ IVF-ICSI • Embryo culture • Extended embryo culture

Advanced ART Techniques• Assisted hatching (laser and chemical [acidified Tyrode’s] method)• Embryo biopsy

Pre-Implantation Genetic Diagnosis• Blastomere biopsy• Biopsy of mouse/hamster 8-cell stage embryos• Use of laser and acid Tyrode’s solution for zona drilling• Blastomere and polar body aspiration• Cell spreading• Fixation techniques

Cryopreservation• Embryo cryopreservation (mouse embryos)• Vitrification

Andrology• Sperm preparation for IVF/ICSI

Virtual Tour of Facilities

MODULE 5

Module 5A:IVF/ICSI/Micromanipulation (80 hours)

Module 5B:ICSI/Micromanipulation (40 hours)

Module 5C:Advanced ART Techniques (40 hours)

Module 5D*:Customizable ART Training (40 - 320 hours)

*How Module 5D Works:1) Candidates select a) the techniques they wish to learn (Modules 1-8); and b) the duration of their training (1- 8 weeks).

2) Candidates consult with the Course Director regarding their training needs and choices, and together, they will decide on the appropriate modules and duration required by the candidate

3) A personalized training schedule is created specifically for the candidate

4) Training is provided at a pace established by the candidate and is geared towards fulfilling the candidates personal needs.

ART Trainee

taking Module 5

will receive a

FREE copy of

an ART Book

and more...

Amer

ican

Center for Reproductive Medicine

Centrum Excellentiae Est.

1993

Ranked One of America’s Top Hospitals

American Center for Reproductive MedicineHands on Training in Assisted Reproductive Technology

1 | Cleveland Clinic American Center for Reproductive Medicine Learn more about our ART Training Program

Clinical Didactics by a Reproductive Endocrinologist & Male Infertility Specialists

1) Evaluation and management of male infertility2) Lectures on latest strategies for controlled ovarian hyperstimulation and monitoring of stimulated cycles 3) Oocyte pickup & Embryo transfer: Clinical inputs

ART Training

2 | Cleveland Clinic American Center for Reproductive Medicine Learn more about our ART Training Program

Isaac Glatstein, MDMedical DirectorConceive NJ

Rizwan Malik, MD, MSc, FACOG, FACSIVF SpecialistHouston, Texas

Sajal Gupta, MS, MD, TS (ABB) (MS in Reproductive Clinical Science, Jones Institute)Reproductive SpecialistTechnical Supervisor, Andrology Laboratory Assistant Professor, Lerner College of Medicine

Neel Parekh, MD Male Infertility Specialist Associate Staff, Department of Urology, Cleveland Clinic

Rakesh Sharma, PhDAssistant Technical Supervisor, Andrology LaboratoryAmerican Center for Reproductive MedicineAssociate Professor, Lerner College of Medicine

Sarah Vij, MDDirector, Center for Male Fertility Associate Staff, Department of UrologyCleveland Clinic

Ashok Agarwal, PhD, HCLDDirector, Andrology CenterDirector, American Center forReproductive MedicineProfessor, Lerner College of Medicine

Christina Anagnostopoulou, MSDirector and Clinical EmbryologistIVF Clinic “Embryo ART”Athens, GreeceTestimonial

Israel Maldonado Rosas, MScDirector and Clinical EmbryologistCitmer IVF ClinicMexico City, MexicoTestimonial

Semenology - Modules 1A (8 hours) and 1B (16 hours)Gain proficiency in:

Module 1A: • Collection and delivery of semen sample • Routine semen analysis • Computer assisted semen analysis (CASA) • Review quality control methods for semen analysis • Proficiency testing and reporting for semen analysis

Module 1B: • Sperm morphology assessments by WHO (4th and 5th Ed.) and Kruger’s strict criteria • Differences between the two morphology classification systems • Morphology smear preparation and practice staining techniques • Quality control measures

ART Sperm Preparation - Module 3 (16 hours)Develop skills in sperm preparation for ART: • Preparation of ejaculated sperm for ART: Sperm wash Double density gradient technique Swim-up protocol • Method of retrieval of sperm from retrograde ejaculate (demonstration only) • Sperm retrieval and preparation of testicular biopsy specimens (using rat or mouse specimens)

Advanced Andrology Techniques - Module 2A (40 hours) and 2B (80 Hours)Gain competency in these advanced techniques:

Module 2A (40 hours) • Measurement of sperm DNA fragmentation (SDF) by TUNEL assay using benchtop flow cytometer • Measurement of Oxidation Reduction Potential (ORP) in semen samples • Measurement of Reactive Oxygen Species (ROS) levels in semen samples • Measurement of Total Antioxidant Capacity (TAC) in seminal plasma • Selection of non-apoptotic spermatozoa using MACS* technique

Module 2B (80 hours) • Assessment of sperm DNA fragmentation (SDF) by TUNEL assay with BD Accuri C6 Benchtop Flow Cytometer • Measurement of Oxidation Reduction Potential (ORP) in semen samples • Measurement of Reactive Oxygen Species (ROS) levels in semen samples • Measurement of Total Antioxidant Capacity (TAC) in seminal plasma • Selection of non-apoptotic spermatozoa using MACS* technique

*Magnetic-activated cell sorting

Cryopreservation - Module 4 (8 hours)Learn the proper techniques for freezing human spermatozoa: • Freezing of ejaculated human spermatozoa • Cryopreservation of epididymal and vas deferens aspirates* • Isolation and cryopreservation of sperm from testicular biopsy specimens* • Protocol for cryopreservation of ovarian tissue from cancer patients**demonstration only

Management of IVF and Andrology Laboratories - Module 7 (16 hours)Gain proficiency in: • Implementation of a Total Quality Management Program • Regulation, licensing and accreditation • Quality Control and Quality Assurance • Proficiency testing and competency assessment • Quality management tools • Troubleshooting in fertility lab

Vitrification - Module 6 (8 hours)Acquire competency and knowledge of vitrification: • Fundamentals of vitrification • Vitrification for routine use in an embryology laboratory • Hands on training in set-up for vitrification of oocytes and embryos using techniques such as: open and closed vitrification system, warming of cryopreserved oocytes and embryos, oocyte and blastocyst vitrification, cleavage stage embryo vitrification

Preparing for CAP Accreditation and FDA Inspection - Module 8 (16 hours)Learn how to prepare your laboratory or tissue bank for accreditation by the Collegeof American Pathologists (CAP) Reproductive Laboratory Inspection Program • Learn how to prepare your reproductive laboratory for accreditation by the College of American Pathologists (CAP) inspection program or your tissue bank in the United States: Food and Drug Administration (FDA) inspection. • What to do: Pre-inspection, during inspection and post inspection • Inspection Techniques • Quality Management (pre-analytic, analytic, and post-analytic processes) • Critical indicators for all three processes • Laboratory General Checklist • Reproductive Laboratory Common Checklist (Embryology and Andrology) • Team Leader assessment • FDA Compliance and Registration • Common Checklist

Training Faculty

AccreditationAccredited for 320 AMA PRA Category 1 CreditsTM

Participants claiming CME credit from this activity may submit the credit hours to the American Osteopathic Association Council on Continuing Medical Education for Category 2 credit.Accredited by the American College of Embryology

Learn MoreFor complete information, application instructions and course fees, visit www.clevelandclinic/org/reproductiveresearchcenter/arttraining.html

Virtual tour of the American Center for Reproductive Medicine

Training Methodology:1. Training provided by certified, practicing Clinical Embryologists

2. Trainers include Andrologists, Reproductive Endocrinologists and other experts in ART

3. Fully hands-on, one-on-one laboratory training sessions under the direct guidance of Clinical Embryologists • Training is done using animal models (mouse embryos) (Module 5)

4. Each training session is customized to the trainee’s: • Professional background • Competence and skill level • Specific training needs • Desired outcomes from attending the ART training course

5. Interactive discussion and teaching sessions

6. Live lectures and multimedia presentations in small group sessions

7. Four state-of-the-art micromanipulators dedicated solely for training purposes (IVF/ICSI)

Note:• Training is offered in a dedicated ART training suite within a non-clinical research laboratory• There is no patient contact

Trainee Evaluations:Each Trainee is tested at the end of their training for:

1. Knowledge/Theoretical Component (30%): Multiple choice questions on lab techniques linked to each course module

2. Practical ART Skills: Tested on specific lab techniques taught during the training

3. Scientific Presentation & Comprehension Skills/Integrative Component (20%):

• The performance of the candidate throughout the course will be reflected in a Grading Report.

• Candidates who meet the training requirements and successfully complete the course work will be awarded a Training Certificate

Registered Trainees Will Receive…1. Course Syllabus with Reading Material and Embryology Protocols

2. Free Access to the Cleveland Clinic Alumni Library

3. Free Text Book on “In Vitro Fertilization: A Textbook of Current and Emerging Methods and Devices, 2nd Edition” edited by Dr. Peter Nagy et al, Springer (only for Modules 5A-5D)4. Free E-Book titled “Andrological Evaluation of Male Infertility: A Laboratory Guide” edited by Dr. Ashok Agarwal et al, Springer (only for Modules 1-4) (on Flash Drive)

5. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction: Fifth Edition (on Flash Drive)

6. Certificate of Training (issued upon successful assessment by the instructor)

7. Daily Lunch Meal Tickets

This certificate is presented to:

American Center for Reproductive Medicine The Cleveland Clinic

has receivedHands on Training in Human Assisted Reproduction

(Module 5B: ICSI, Micromanipulation, Embryo Biopsy and Assisted Hatching) for 40 hours offered by the

American Center for Reproductive Medicine, Cleveland Clinic September 25th - 29th, 2019

Caryn Costa, MScRio de Janeiro, Brazil

MANESH KUMAR PANNER SELVAM, Ph.D. Coordinator of Research & ART Training Department of Urology American Center for Reproductive Medicine Cleveland Clinic

ASHOK AGARWAL, Ph.D., HCLD (ABB) Professor & DirectorDepartment of Urology American Center for Reproductive Medicine Cleveland Clinic

ISRAEL MALDONADO ROSAS, MSc ART Training Faculty, American Center for Reproductive Medicine Director and Clinical EmbryologistCitmer IVF Clinic, Mexico City, Mexico

CHRISTINA ANAGNOSTOPOULOU, MSc ART Training Faculty, American Center for Reproductive Medicine Director and Clinical EmbryologistIVF Clinic “Embryo ART”Athens, Greece

Scholarship in Teaching AwardTraining Program in Advanced Reproductive Techniques

The Case Western Reserve School of Medicine Scholarship in Teaching Awards is an annual program designed to recognize faculty contribution to education from the preceding year and promote undergraduate, graduate, and continuing medical education teaching as a scholarly activity.

Teaching approaches considered scholarly are characterized • Purposeful planning • Systematic implementation and • Reflection and assessment for improvement.

The result should be an academic product that is actively shared and that will support the development of a community of teaching scholars.

This award was given out at the Case Medical School Education Retreat on March 12, 2015. ACRM’s hands-on ART training program has previously been twice-recognized with this prestigious Scholarship in Teaching Award, in 2013 and again in 2014.

Certificate of Training Awarded Upon the Successful Completion

of the Hands on ART Training

This certificate is presented to:

American Center for Reproductive Medicine The Cleveland Clinic

has receivedHands on Training in Human Assisted Reproduction

(Module 5A: IVF/ICSI, Micromanipulation, Embryo Biopsy, Assisted Hatching and PGD) for 80 hours offered by the

American Center for Reproductive Medicine, Cleveland Clinic September 9th - 20th, 2019

Otto Umana, MDFayetteville, North Carolina

MANESH KUMAR PANNER SELVAM, Ph.D. Coordinator of Research & ART Training Department of Urology American Center for Reproductive Medicine Cleveland Clinic

ASHOK AGARWAL, Ph.D., HCLD (ABB) Professor & DirectorDepartment of Urology American Center for Reproductive Medicine Cleveland Clinic

ISRAEL MALDONADO ROSAS, MSc ART Training Faculty, American Center for Reproductive Medicine Director and Clinical EmbryologistCitmer IVF Clinic, Mexico City, Mexico

CHRISTINA ANAGNOSTOPOULOU, MSc ART Training Faculty, American Center for Reproductive Medicine Director and Clinical EmbryologistIVF Clinic “Embryo ART”Athens, Greece

AAmmeerriiccaann CCeenntteerr ffoorr RReepprroodduuccttiivveeMMeeddiicciinnee AARRTT TTrraaiinniinngg

This document reflects the performance of the candidate during the 2019 ART Training Session: (Module 5A: IVF/ICSI, Micromanipulation,

Embryo Biopsy, Assisted Hatching and PGD)This is to attest that the trainee,

Trevor Lynn Bond, DOhas successfully completed the course work prescribed by the Cleveland Clinic’s

American Center for Reproductive Medicine and is therefore recommended for the award of the ART Training Certificate

Grading Report

Grading Scale: A+ (>96%), A (91-95%), A- (86-90%), B+ (81-85%), B (76-80%), B- (71-75%), C (<70% is Fail)

Total Marks Marks Obtained (%) Percent Contribution

30 82 25

Practical Component; Skill Test (50%)

50 98 49

Integrative Component (20%) Presentation and Viva Voce

20 97 19

FINAL GRADE 93%

ISRAEL MALDONADO ROSAS, MSc ART Training Faculty

MANESH KUMAR PANNER SELVAM, Ph.D Coordinator, ART TrainingSeptember 13th, 2019

ASHOK AGARWAL, Ph.DProgram Director

Theoretical Component; MCQ (30%)

CHRISTINA ANAGNOSTOPOULOU, MSc ART Training Faculty