assessment of genetic hotspots for phytophthora - agro.au.dk · development of late blight...
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Development of Late Blight (Phytophthora infestans) Resistant Potato Breeding Material for Organic Farming
Assessment of genetic hotspots for Phytophthora resistance and their use as molecular markers
in potato breeding
BLE-Projekt 2810OE071 (LfL), 2810OE121 (JKI), 2810OE122 (IPK)
K. Sieber, G. Forster, A. Berger, A. Schwarzfischer und A. Kellermann
Aims and objectives
Fotos: JKI Dr. Karen Sieber 2
Field Trial: 150 cultivars and breeding clones have been grown and
examined under organic farming conditions.
Molecular Analyses: Examination of resistance hotspots and evaluation of genetic
markers for resistance breeding
Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative
approach on organic farms
Fotos: JKI Dr. Karen Sieber 3
Field Trial: 150 cultivars and breeding clones have been grown and
examined under organic farming conditions.
Molecular Analyses: Examination of resistance hotspots and evaluation of genetic
markers for resistance breeding
Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative
approach on organic farms
Aims and objectives
Field trial
Fotos: JKI
Varieties for field trials 2014 Total: 153 cultivars • JKI (28) • LfL (26) • IPK (10) • German potato breeders (58) • others (26) • reference (5)
Dr. Karen Sieber 4
Fotos: JKI
GL: Groß Lüsewitz UE: Uelzen ND: Neuburg a.d. Donau) FS: Freising LL: Landsberg am Lech
natural infection / organic trial field
artificial infection
pest control / maturity test
GL
FS LL
ND
UE
Dr. Karen Sieber 5
Field trial
Fotos: JKI
year number of varieties
environments trials per environment
individuals per plot
2012 117 3 (LL, ND, UE)
2 10
2013 149 1 (FS)+ 3 (ND, LL, UE)
2 10
2014 153 1 (FS) + 3 (LL, ND, UE)
2 10
Parameter Sprouting time development growth form maturity late blight yield
tuber shape
tubers per plant
starch content tuber size
quality frying quality
Dr. Karen Sieber 6
Field trial
Fotos: JKI
Trial field in Schrobenhausen 2012
Dr. Karen Sieber 9
Field trial
Fotos: JKI Dr. Karen Sieber 10 Fotos: JKI
UE: Uelzen ND: Neuburg a.d. Donau) LL: Landsberg am Lech
other
EU_13_A2
LL
ND
UE
Field trial: Phytophthora Monitoring
Fotos: JKI Dr. Karen Sieber 11
days to maturity
days to maturity
LL 2014 Walleshausen LK Landsberg
days to maturity
Δ
rAU
DPC
Δ
rAU
DPC
UE 2014 Natendorf LK Uelzen
Corrected Late Blight Resistance and Maturity for Black’s Differential Set
Field trial
Fotos: JKI Dr. Karen Sieber 12
Field trial
GD 5%
GD 5%
Saka6
Kuras
Hubal
TuebingerCirce
Biogold
Vitabella Bionica
Carolus
Toluca Axona
SarpoMira
-0,4
-0,3
-0,2
-0,1
0,0
0,1
0,2
80 90 100 110 120 130 140
∆rAU
DPC
maturity [days]
Late Blight Resitance and Maturity 2012-2014
JKI Population Anuschka Princess Lolita Jelly Ditta
Aims and objectives
Fotos: JKI Dr. Karen Sieber 13
Field Trial: 150 cultivars and breeding clones have been grown and
examined under organic farming conditions.
Molecular Analyses: Examination of resistance hotspots and evaluation of genetic
markers for resistance breeding
Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative
approach on organic farms
Potato breeding
Fotos: JKI Dr. Karen Sieber 14
Seedling potatoes in Landsberg 2014
Potato breeding
Fotos: JKI Dr. Karen Sieber 15
Participative selection of breeding clones in Landsberg 2014
Potato breeding
Fotos: JKI Dr. Karen Sieber 16
Susceptible plants will be sorted out
Potato breeding
Fotos: JKI Dr. Karen Sieber 17
Harvest of preselected clones in Landsberg n 2014
Potato breeding
Fotos: JKI Dr. Karen Sieber 18
num
ber o
f clo
nes
trial set 2012-2014
Comparison of Late Blight Resistance Values
breeding clones LL 2014 Resistance values (1=resistant, 9=susceptible)
Potato breeding: resistance vs yield
Fotos: JKI Dr. Karen Sieber 19
Aims and objectives
Fotos: JKI Dr. Karen Sieber 20
Field Trial: 150 cultivars and breeding clones have been grown and
examined under organic farming conditions.
Molecular Analyses: Examination of resistance hotspots and evaluation of genetic
markers for potato breeding
Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative
approach on organic farms
Molecular Analyses: Wild Resistance Donors
Fotos: JKI 21
S. demissum
S. stoloniferum
S. pinnatisectum
S. bulbocastanum
S. acaule S. phureja
Fotos: JKI 22
STAos2 45/XI
Molecular Analyses: Resistance Loci
Fotos: JKI Dr. Karen Sieber 24
S. demissum R1
IV
Molecular Analyses: Markers for S. demissum
R3
XI • We applied regions from R1
and R3b as molecular markers.
• Sequences are identical for all investigated cultivars.
• R1 appears in ca. 12% of all trial samples
• R3b appears in ca. 35% of all trial samples
Products: R1, R3a, R3b References: Huang et al. 2005, Rietman 2011, Lokossou et al. 2009; Kim et al. 2012
Fotos: JKI Dr. Karen Sieber 25
S. bulbocastanum
Molecular Analyses: Markers for S. bulbocastanum
• Markers Blb3 and Abpt were established for the blb3 region
• Blb1-820 was used for the blb1 region
• 9% of all samples were positive at the blb3 locus
• 1 sample was positive at the blb1 locus.
Products: − Blb3, Abpt, − RB-629, Blb1-820, RB-226,
Sto1 References: − Lokossou et al. 2009; Kim et
al. 2012; − Fadina et al. 2009; Zhu et al.
2012
blb3
IV
blb1
VIII
Fotos: JKI Dr. Karen Sieber 26
Molecular Analyses: Markers for S. bulbocastanum
GD 5%
GD 5%
04.5214.03 blb3
04.5214.04 blb3 + blb1
05.5049.10 blb3
07.1018.05 blb3 93.6984.07 blb3
94.7082.15 blb3
96.7395.03 blb3
97.7550.02 blb3 98.7826.02 abpt
-0,40
-0,30
-0,20
-0,10
0,00
0,10
0,20
80 90 100 110 120 130 140
∆rAU
DPC
Maturity [days]
Late Blight Resistance Values
Anuschka Princess Lolita Jelly Ditta JKI
Molecular Analyses: Wild Resistance Donors
Fotos: JKI 27
S. bulbocastanum
S. stoloniferum
??
S. andigena
??
Fotos: JKI Dr. Karen Sieber 28
Molecular Analyses
n=38 n=9 n=56
n=9
n=14
-0,15
-0,10
-0,05
0,00
0,05
0,10
0,15
ohne Marker R1 R3b R1 + R3b blb3 / abptΔrAU
DPC
Late Blight Resistance Values
Perspectives
Fotos: JKI Dr. Karen Sieber 29
• S. venturii • S. phureja
IX • Continue breeding efforts and selection of
breeding clones
• Analyse of SNP results
• Find molecular markers for other Solanum species that were used as resistance donors
• Develop markers that allow to distinguish between resistance genes and their inactive homologues
Thank You!
Fotos: JKI Dr. Karen Sieber 30
Georg Forster
Adi Kellermann
Andrea Schwarzfischer
Andi Berger
Project Team
Fotos: JKI Dr. Karen Sieber 31