atnap, a nac family trancription factor, has an important role in leaf senescence
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学术报告 — 文献阅读汇报. AtNAP, a NAC family trancription factor, has an important role in leaf senescence. 报 告 人:王爽 专业年级:食品科学 2011 级 指导老师:寇晓虹副教授. Result and Conclusion. Introduction. Discussion. 1. 2. 3. 4. Outline. Experiment content. TF. - PowerPoint PPT PresentationTRANSCRIPT
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报 告 人:王爽专业年级:食品科学 2011 级指导老师:寇晓虹副教授
AtNAP, a NAC family trancription factor,has an important role in leaf senescence
学术报告—文献阅读汇报
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Outline
IntroductionIntroduction11
22
Result and ConclusionResult and Conclusion33
DiscussionDiscussion44
Experiment contentExperiment content
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Introduction1.1 Leaf Senescence
Transcription FactorsTranscription Factors
TFTF 能够结合在某基因上游特异核苷酸序列上的蛋白质,活化后从胞质转位至胞核,通过识别和结合基因启动子区的顺式作用元件 , 启动和调控基因表达。
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1.2 NAC family transcription factor
Introduction
WRKY
DREBbZIP
NACMYB
NAC
含有 NAC 结构域的蛋白统称为 NAC 蛋白
植物生长发育植物生长发育激素调节激素调节
环境胁迫应答环境胁迫应答
NAMNAM
CUC2CUC2
ATAF1/2ATAF1/2
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Experiment content Inducible overexp-
ression of AtNAP
AtNAP expression in T-DNA line
Subcellular location of AtNAP
AtNAP expressionduring leaf senescence
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3.1 AtNAP is upregulated during leaf sencence in Arabidopsis
Results and Conclusion
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3.2 AtNAP is targeted to nuclei
Results and Conclusion
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3.3 The AtNAP expression is knocked out in one T-DNA line and knocked down in another line
Results and Conclusion
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3.4 Leaf senescence is significantly delayed in the atnap null mutant plants
Results and Conclusion
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Results and Conclusion
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Results and Conclusion
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3.5 AtNAP homologs in rice and kidney bean are specifically expressed in senescing leaves
Results and Conclusion
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Results and Conclusion
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3.6 AtNAP restores the atnap null mutant plants to wild type
Results and Conclusion
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3.7 Inducible overexpression of AtNAP causes precious senescence
Results and Conclusion
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Results and Conclusion
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3.8 Microarray analysis of the expression profile of AtNAP
Results and Conclusion
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RT-PCRRNA gel blot
T-DNA insertionsPlasmid constructionPlant transformationComplementation test
Disscussion
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1.冶晓芳 ,唐益苗 ,高世庆 ,等 .植物NAC转录因子的研究进展 . 生物计数通报 ,2009,10(6)20–24.2.李鹏 ,黄耿青 ,李学宝 .植物NAC 转录因子 . 植物生理与分子生物学 ,2010,46(3)294–300.3.郭玉娟 ,魏强 .高羊茅中衰老相关转录因子AtNAP同源基因的克隆及功能分析 . 复旦学报 ,2010,49(5)544–558.4.彭辉 ,于兴旺 ,成慧颖 ,等 .植物NAC转录因子家族研究概况 . 植物学报 ,2011,45(2)236–248.报 ,2011,45(2)236–248.5.史红飞 ,高翔 ,陈其皎 , 等 .小麦NAC 转录因子的基因克隆与序列分析 . 西北农林科技大学学报 ,2011,31(3)395–401.6.易素华 ,张 丞 ,史志浩 ,等 .拟南芥花粉表面蛋白基因T-DNA插入突变体的鉴定 .上海师范大学学报 ,2010,39(2)313–318.7.尤扬 ,叶永忠 ,等 .植物激素对黄姜叶片Fv/Fo和Fv/Fm 的影响 .安徽农业科学 ,2007,35(19)5707–5703,5744.
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