Supplementary Figure IV. Validation of mouse TspanC8 tetraspanin RT-PCR primers. The relative mRNA levels of TspanC8 tetraspanins were measured by RT-PCR from a mixture of cDNAs from mouse brain, heart, kidney, liver, lung, muscle and thymus tissue. Standard curves were used for each primer and primer efficiencies obtained using the MxPro RT-PCR software from Stratagene (error bars represent the standard error of the mean from two experiments, each done in duplicate).

Average % primer

efficiency: 92.0 111 94.3 96.7 98.6 110

100

50

0

75

25Ts

pa

n5

Tsp

an

10

Tsp

an

14

Tsp

an

15

Tsp

an

17

Tsp

an

33

Rel

ati

ve

mR

NA

exp

ress

ion