awwa biological treatment symposium 2016_luminultra presentation
TRANSCRIPT
D. Tracey, P.E.
P. Whalen, P.E.
January 2016
Monitoring and Control Capabilities of 2nd Generation ATP Monitoring in
Biological Filters
The GoalSeveral tools exist to quickly and accurately measure chemical and physical properties. What about microbiological measurements?
• Assess microbiological activity quickly and easily
• Measure the total biological population
The GoalA Monitoring and Control Toolbox for Biological FiltersP. Evans, J. Smith - CDM SmithM. LeChevallier, O. Schneider, L. Weinrich, P.. Jjemba - American Water
Good: ATP, hydrolase enzyme activity, HPC, DOMedium: EPS, Terminal Restriction Fragment Length Polymorphism (T-RFLP)Poor: O2 Uptake, Electron Transport System Activation
The Basics of ATPATP (Adenosine Triphosphate) is a molecule present in all living cells
and is critical to cell growth and function.
ATP tests have been used for decades in the food and medical hygiene industries but were previously not applicable for water or fluid samples.
Main advantages:
• Real-time feedback (< 5 minutes)
• 100% of microbes detected
• Versatile (bulk fluid or attached growth)
Now applicable for water, wastewater, oil and gas, chemical products and solids (biofilter media).
The Basics of ATP
The Reaction
Adenosine Triphoshate Luciferin-Luciferase Light
ATP Analysis
Step 1: Acquire sample
What should be sampled?
Filter media or
influent/effluent water?
Frequency?
Bulk Water vs Media AnalysisFilter effluent testing tends to be easier due to simpler sample acquisition, but for direct indication of biological capacity on the filter, test the media.
• High water ATP could indicate sloughing of biomass from media.
• Low water ATP from a healthy filter does not accurately reflect a firmly- attached population.
Bulk Water vs Media Analysis
• Media testing tends to be the most direct way to assess filter biomass content.
• Analyze water to assess downstream loading. (e.g. disinfection, membranes)
The Analysis
Step 2: ATP Extraction + Dilution
The Analysis
Step 3: Assay
Applications
• [ATP] Total biological activity∝
• Filter capacity can be assessed in terms of available biomass.
• Monitor pre- and post-backwash to help establish operating targets.
Applications
• Gain direct insight into biomass’ response to process changes:
Nutrient supplementation Pre-oxidation/Chlorinated backwash Temperature/seasonal changes
Biomass Acclimation Example
16-Nov-2012 3-Apr-2013 9-Apr-2013 12-Apr-2013 18-Apr-2013 25-Apr-2013 2-May-20130
100,000
200,000
300,000
400,000
500,000
600,000
700,000
800,000
900,000
30,2
63
54,2
72
69,4
17
103,
970
364,
084
385,
551
589,
257
6,88
8
7,81
7
13,8
80
17,3
48
Start of Run
End of Run
ATP
(pg/
g)
Pre-chlorination shut off
ND
ND
ND
Results courtesy of Dalhousie University (Halifax, NS, Canada)
Biofilter Profile
Results courtesy of Dalhousie University (Halifax, NS, Canada)
Biofilter Profile
Results courtesy of Dalhousie University (Halifax, NS, Canada)
HP, pH =
9
LP, pH =
9
Control
HP, pH =
6
LP, pH =
60
400,000
800,000
1,200,000
1,600,000
ATP
(pg/
g)
Summary• Quantify biological activity quickly
• Establish cause-and-effect relationships between key process variables and biomass activity within the filter
• Use this feedback together with process data to assess its impact and optimize operating conditions
Acknowledgements• Dalhousie University
• D. Graham Gagnon• Heather Granger• Amina Stoddart
• Water Studies Group• NSERC• Halifax Water• CBCL• Cape Breton Regional Municipality
Dave Tracey, P.E. LuminUltra [email protected]+1 (506) 459-8777 ext. 213
Monitoring and Control Capabilities of 2nd Generation ATP Monitoring in
Biological Filters