bacterial growth - 21umas-edu.org...bacterial growth curve 1- lag phase: -metabolite-depleted cells...
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BACTERIAL GROWTH
drJamil taher 2
Refers to an increase in bacterial cell
number (multiplication).
Results from bacterial reproduction
(binary fission)
parameter called generation time
(the average time required for cell
numbers to double).
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Bacterial Reproduction
Bacteria reproduce a sexually by simple binary
fission.
1) Elongation of the bacterial cell.
2) Duplication of the chromosome.
3) The two sister chromosomes attach to the
mesosome.
4) Cell membrane and cell wall will form a
transverse septum that divides the mother cell
into 2 equal daughter cells.
drJamil taher 3
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drJamil taher 4
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Bacterial Reproduction
o The number of bacteria (n) increases logarithmically.
1 2 4 8 16 32 64 128 etc
=2n
The doubling (generation) time: The time required by the bacteria to double its number,
varies from one species to another.
drJamil taher 5
The generation time varies from one species to
another e.g.
1-V. cholera has a generation time 17 minutes.
2-Escherichia coli has a generation time 20 minutes.
By contrast,
3-Mycobacterium tuberculosis has a generation time of 24
hours.
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drJamil taher 6
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Growth bacteria
.
Most bacteria will grow on artificial culture media prepared from
extract from animal or plant tissues, which supply pre-formed
nutrients and vitamins
Some bacteria, cannot grown in vitro;
e.g.
1- Mycobacterium leprae (leprosy)
2-Treponema pallidum (syphilis),
Other bacteria, only replicate intracellular within host cells
and are therefore grown in tissue culture. e.g.
1-Chlamydia spp.
2-Rickettsia spp.,
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Growth Requirements
o In order to grow bacteria have certain requirements:
Nutrients
Growth factors
Suitable temperature
Suitable pH
Oxygen
Carbon dioxide
Moisture.
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Nutrients
o Nutritional requirement for growth include:
1- Macromolecules: needed in larger amounts.
Examples:
- CHNOPS
- Mineral salts: Ca, Fe, Mg, k,..
2- Micromolecules: required in trace amounts.
- Mn, Zn, Co, Ni
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Bacteria are classified into:
1- Autotrophic bacteria: (auto= self/ troph=
feeding)
Autotroph= use carbon dioxide as a source of
carbon and ammonium salts as a source of
nitrogen, from which they synthesize organic
substances, e.g protein, CHO,…
drJamil taher 10
Nutritional requirements
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2- Heterotrophic bacteria: (hetro= other)
Use preformed organic compounds (made by
others)
they derive their energy by oxidation or
fermentation of organic compounds (glucose).
drJamil taher 11
Nutritional requirements
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Most bacteria of medical importance
are heterotrophic bacteria
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Growth factors
oMany pathogenic species of bacteria require for
growth essential substances, which they can not
synthesize, like vitamins, amino acids,…
Fastidious bacteria: are those which require
unusually complex nutrients.
drJamil taher 13
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Temperature
Different microbial species vary widely in their
optimal temperature ranges for growth:
Psychrophilic:
grow best at low temp (15 -20°C)
Mesophilic:
grow best at 30 -37° C.
Thermophilic forms:
grow best at 50 - 60°C
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drJamil taher 15
Temperature
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Hydrogen ion concentration (pH)
Most bacteria of medical importance can grow
at a pH of 6-8 (neutralophiles).
Some forms (acidophiles) have optima as low
as pH 3.0
,e.g lactobacilli.
Others (alkaliphiles) have optima as high as pH
10.5,
e.g. Vibrio cholerae.
drJamil taher 16
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pH
Acidophiles
Neutralophiles
(most bacteria)
Alkalophiles
drJamil taher 17
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Examples Definition Class
Mycobacterium
tuberculosis Grow only in the
presence of O2
Obligate (strict) aerobe
Clostridium
tetanus
Can not grow in
the presence of O2
Obligate (strict)
anaerobe
Most pathogenic
bacteria of
medical
Can grow in the
presence or
absence of O2
Facultative anaerobe
Campylobacter Require low O2
tension
Microaerophilic bacteria
Oxygen
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CO2
Most bacteria require CO2 in low concentration.
Certain bacterial species (Carboxyphilic) require
higher concentrations of CO2, e.g.:
- Neisseria species require 5-10%.
- Brucella abortus require 20% CO2.
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Moisture
Four-fifth of bacteria cell weight consists of
water, and moist is necessary for growth.
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Bacterial Growth Curve
If a small number of bacteria are inoculated into
a liquid nutrient medium and the bacteria are
counted at frequent intervals and the results
plotted, a characteristic growth curve with 4
phases is obtained:
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Bacterial Growth Curve
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Bacterial Growth Curve
1- Lag phase:
-Metabolite-depleted cells adapt to new environment.
-No cell division (constant number).
- The bacteria form the enzymes and molecules needed for replication.
2- Logarithmic (exponential) phase:
- Rapid cell division occurs.
- The number of living bacteria increases by time.
- Clinical significance: this phase = symptoms and signs of the disease.
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3- Stationary phase:
- Nutrients are exhausted.
- Waste products are accumulated (toxic products).
- The number of dying cells = number of new cells
- (The number of living bacteria remains
constant).
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Bacterial Growth Curve
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4- Decline phase:
- Nutrients are more exhausted.
- Waste products are more accumulated.
- The number of dying cells > number of new
cells.
The number of living bacteria decreases by
time.
o Clinical significance: this phase = recovery
and convalescence.
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Bacterial Growth Curve
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Bacterial Metabolism
Many bacteria secretes enzymes(lipases,
nucleases, proteinases,..) that break down the
nutritive material in to simpler molecules.
Theses molecules are then oxidized by bacteria
to yield energy, and the degradation products
are used to build up structural components and
essential molecules for cell metabolism.
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Depending on the biochemical mechanisms
used, bacterial metabolism can be categorized in
to :
OXIDATION
- Aerobic respiration.
- An aerobic respiration.
Fermentation.
drJamil taher 27
Bacterial Metabolism
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Bacterial Metabolism
Oxidation:
Removal of electrons and hydrogen ions.
The substrate to which hydrogen ions are released is
called H acceptor.
In aerobic respiration the hydrogen acceptor is oxygen.
This results in the production of toxic substances e.g
hydrogen peroxide (H2O2) and (superoxide).
Aerobes and facultative anaerobes; contain certain
enzymes , e.g. catalase and superoxide dismutase which
degrade these toxic compounds and protect bacteria
from their effect.
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Bacterial Metabolism
In the anaerobic respiration the ultimate
hydrogen acceptor is an inorganic compounds
(not hydrogen), because these bacteria does not
contain protective enzymes.
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Fermentation: - Refers to the break down of sugar to pyruvic
acid and then usually to lactic acid.
- Fermentation is also called glycoltic cycle, and
this is the process by which facultative bacteria
generate ATP in absence of oxygen.
- If oxygen present, the pyruvate produced by
fermentation enters the Krebs cycle bacteria
and generates CO2 and water.
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Bacterial Metabolism
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The fermentation of certain sugars is the basis of
the laboratory identification of some pathogens.
The acids produced lower the pH, and this can be
detected by the change in color of indicator dyes.
drJamil taher 31
Bacterial Metabolism