Khoa CNTP - HUFIThs. Trng Bch Chin 11PHN TCH THC PHMTRNG I HC CNG NGHIP THC PHM Tp HCMKHOA CNG NGH THC PHM2GiI THIU MN HCGing vin: ThS. TRNG BCH CHINPhone: 01686.151.042Email: truongbachien@yahoo.comtruongbachchien2011.co.ccKhoa CNTP - HUFIThs. Trng Bch Chin 23Gii thiu v ni dung mn hc4 Chng 6: Xc nh Lipit 64 Chng 5: Xc nh Protit 54 Chng 4: nh lng CacbonHydrat 46 Chng 3: nh lng tro, m v khong 32 Chng 2: nh lng acid 210 Chng 1: Cc khi nim c bn 1S tit Ni dung Stt4Ti liu tham kho[1] Gio trnh l thuyt phn tch thc phm- Khoa CNTP- trng i hccng nghip thc phm TpHCM[2] Bi Th Nh Thun, Kim nghim lng thc thc phm, Nh xutbn Khoa hc K thut , 1990.[3] S.Suzanne Nielsen, Food Analysis Second Edition,Gaithersburg,Maryland, 1998.[4] Trng Bch Chin, gio trnh phn tch cng nghip,Trng Caong Cng nghip TpHCM, 2004[5] Trng Bch Chin, Phn tch cng nghip thc phm, Trng iHc Cng nghip TpHCM, 2008 Khoa CNTP - HUFIThs. Trng Bch Chin 35CHNG 1: Cc khi nim c bn1.1. Cng thc nng 1.2. Pha ch dung dch1.3. Cc phng php phn tch6CHNG 1: Cc khi nim c bn1.1. Cng thc tnh nng 1.1.1. Nng phn trm khi lng - C (%): biu dins gam cht tan c trong 100 gam dung dch100mm% Cddct =Vi:mct- s gam cht tan (g)mdd- s gam dung dch (g)C% - nng phn trm ca dung dchKhoa CNTP - HUFIThs. Trng Bch Chin 47CHNG 1: Cc khi nim c bn1.1. Cng thc tnh nng 1.1.2. Nng mol CM (M): biu din s mol cht tan ctrong mt lt dung dch.VnCM=Vi:n- s mol cht tan (mol)V - th tch dung dch (l)CM- nng mol/l (M) 8CHNG 1: Cc khi nim c bn1.1. Cng thc tnh nng 1.1.3. Nng ng lng CN (N): biu din s ng lnggam cht tan c trong mt lt dung dch.V' nCN=Vi:n - s ng lng gam cht tanV - th tch dung dch (l)CN- nng ng lng (N) Khoa CNTP - HUFIThs. Trng Bch Chin 59CHNG 1: Cc khi nim c bn1.1. Cng thc tnh nng Mi lin h cc loi nng trn c cho bi cc biu thc: Trong : d: khi lng ring ca dung dch (g/ml)M: phn t lng ca cht tan: ng lng gam cht tan (lg) Md 10% C CM =CM C%d 10% C CN =CN C%CN = z.CMCN CM10ng lng nh lut ng lngNhc nhNit trong N2O, NO, N2O3, NO2, N2O5ln lt c ng lng tng ng l bao nhiu?Cng thc chung ng lng l:zM=Trong acid/baz, z ls ion H+ hay OH- b thay th trong 1 phn t axit hay bazTrong phn t mui, z l tch s ion thay th vi in tch ion thay th (ion c th l cation hoc anion) tnh cho 1 phn t mui Trong hp cht oxy ha kh, n l s electron m mt phn t cht kh c th cho hay mt phn t cht oxy ha c th nhn c tnh cho 1 phn t cht Khoa CNTP - HUFIThs. Trng Bch Chin 611CHNG 1: Cc khi nim c bn1.2. Pha ch dung dch1.2.1. Pha long dung dch:Trng hp 1:m1(g) dung dch A C1 (%) trn vi m2(g) dung dch A C2 (%), thu c m3 (g) dung dch A C3 (%) th c :m1 / m2 = |(C2 C3) / (C1 C3)|Dng quy tc cho:m1 ddA C1 |C2 - C3|C3m2 ddA C2 |C1 C3|12CHNG 1: Cc khi nim c bn1.2. Pha ch dung dch1.2.1. Pha long dung dch:Trng hp 2:V1(mL) dung dch A C1 (M) trn vi V2(mL) dung dch A C2 (M), thu c V3 (mL) dung dch A C3 (M) Dng quy tc cho:V1 ddA C1 |C2 - C3|C3V2 ddA C2 |C1 C3|th c :V1 / V2 = |(C2 C3) / (C1 C3)|Khoa CNTP - HUFIThs. Trng Bch Chin 713 1. Tnh th tch nc ct cn pha vo 100mL dung dch HCl 20% (d= 1,1g/mL) thu c dung dch c nng 5%; bit H=1, Cl=35,5a. 300mLb. 380mLc.330mLd.400mL2. Tnh th tch nc ct cn pha vo 100g dung dch H2SO420% (d= 1,12g/mL) thu c dung dch c nng 5%a.300mLb. 330mLc. 380mLd.400mLV d :14 1. pha ch 100mL dung dchC2H2O4 0,1M, th cn bao nhiu gam lng tinh th C2H2O4.7 H2O , bit C=12; H=1; O=16:a. 2.61 b. 2.36c.2.16 d. 2.63 2. pha ch 100mL dung dchC2H2O4 0,1M, th cn bao nhiu gam lng dung dch C2H2O4 20%( d= 1,24g/mL) , bit C=12; H=1; O=16:a.4.5b. 5.4c. 5.8d. 4.8 3.Tnh khi lng tinh th KMnO4 98% theo l thuyt pha 500mL dung dch KMnO4 0,05N . Bit M(KMnO4) = 158dvCa.1.3435b. 0.1533c. 13.4354d. 0.1443 Khoa CNTP - HUFIThs. Trng Bch Chin 815CHNG 1: Cc khi nim c bn1.2. Pha ch dung dch1.2.2. Pha ch dung dch t cht rnTnh khi lng cn thit pha V(mL) dung dch (A) CM t tinh th rn (A) c tinh khit (p%)MpC Vm .. 10.=16 1. pha ch 100mL dung dchC2H2O4 0,1M, th cn bao nhiu gam lng tinh th C2H2O4.7 H2O , bit C=12; H=1; O=16:a. 2.61 b. 2.36c.2.16 d. 2.632. pha ch 100mL dung dchC2H2O4 0,1M, th cn bao nhiu gam lng dung dch C2H2O4 20%( d= 1,24g/mL) , bit C=12; H=1; O=16:a.4.5b. 5.4c. 5.8d. 4.83.Tnh khi lng tinh th KMnO4 98% theo l thuyt pha 500mL dung dch KMnO4 0,05N . Bit M(KMnO4) = 158dvCa.1.3435b. 0.1533c. 13.4354d. 0.1443V d :Khoa CNTP - HUFIThs. Trng Bch Chin 917 1.3.1. Phng php khi lng1.3.2. Phng php th tch1.3.2.1. Chun acid baz1.3.2.2. Chun phc cht1.3.2.3. Chun oxy ha kh1.3.2.4. Chun to ta1.3.3. Phng php o quang18CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.1. Phng php khi lngNi dungMuddch muTaC. rnKhoa CNTP - HUFIThs. Trng Bch Chin 1019 Ly 5(g) mt mu thc phm ccha P em chuyn ha thnhdung dch, ri tin hnh to tadi dng MgNH4PO4sau khi loi b cc thnh phn khc P. em nung th uc 1,235(g) Mg2P2O7, tnh hm lng %P ctrong mu ban u?(Mg=24; P=31; O=16; N=14; H=1)20TNG QUT Hm lng cu t (x) khi chuyn i tdng mu A (m0) thnh dng cn B (m1) (theo phng trnh chuyn ho: a Ab B) sau khi c pha long (nh mc) n ln V1, V2, ...Vnri trch ra m ln V'1, V'2, ...V'm. Cng thc tnh hm lng % s l? Khoa CNTP - HUFIThs. Trng Bch Chin 1121H s chuyn iBAM bM aK..=F KmmX . . %01=H s pha long'VVF=22 Cn 0,35(g)mt lng mui chaNa2SO4ri nh mc thnh 250mL dung dch. Ht 50(mL) t dung dchny,phn tch theo phng phpkhi lng th c 0,0405(g) BaSO4.Tnh hm lng %SO42c trong mu mui cha Na2SO4ban u.Khoa CNTP - HUFIThs. Trng Bch Chin 1223S chuyn ha cch gii Phn ng chuyn i: Na2SO4 BaSO4H s chuyn i: Vyhm lng % SO42-=% 88 , 94 % 100 . 64 , 1 .50 . 35 , 0250 . 0405 , 0=64 , 1233 . 1142 . 1..= = =BAM bM aK24 1. Ly 5(g) mt mu thc phm c cha P em ta di dng MgNH4PO4ri nung th uc 1,235(g) Mg2P2O7, th hm lng %P , s l bao nhiu?, bit Mg=24; P=31; O=16; N=14; H=1:a.6.8982 %b. 6.8897 %c. 6.8789 %d. 6.8778 %2. Ly 5(g) mt mu thc phm c cha P em ta di dng MgNH4PO4ri nung th uc 1,235(g) Mg2P2O7, th hm lng %P2O5, s l bao nhiu?, bit Mg=24; P=31; O=16; N=14; H=1:a. 15.9817b.15.7991c. 15.8917d. 15.1789 Khoa CNTP - HUFIThs. Trng Bch Chin 1325CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.2. Phng php th tchNi dungMuddch muDd phn tchChun 26CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.2. Phng php th tchNi dungCh thDung dch phn tchThuc thKhoa CNTP - HUFIThs. Trng Bch Chin 1427Ch Ch th im tng ng im cui chun Cng thc tnh kt qu S sai lch trong tnh ton v thc t28V dKhi tin hnh chun 10mLdungdch HCl vich th phenolphtalein,c 9,8mLdungdchNaOH 0,1Nth dungdch HCl i t khngmu sang mu hng nht.1. Xc nh nng dung dch HCl ban u2. Vic chun chnh xc cha? S sai lchl bao nhiu?Nu qua3ln TNth tch caNaOH ln lt l 9,8 9,6 9,2(mL).Bit hs Student t = 4,3 vi tin cy 95%Khoa CNTP - HUFIThs. Trng Bch Chin 1529Cch tnh kt qu nng e e b bV C V C . . =30Tnh s sai lch Ti thiu s ln th nghim Tnh theo phng php thng k ton hc Tnh theo s sai lch ca dng c, thit b Tnh theo s sai lch ca phng phpchun Khoa CNTP - HUFIThs. Trng Bch Chin 1631Ni dungCh thDung dch phn tchThuc thCHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.2. Phng php th tch1.3.2.1. Chun acid - baz32 1. Khi xc nh ch tiu hm lng chua trong thc phm sa, ngi ta dng phng php phn tch th tch g xc nh?a. Phng php chun acid bazb. Phng php chun phc chatc. Phng php chun oxy ha khd. Phng php chun to ta2. Ch th no thng c dng xc nh hm lng acid ton phn?a. Methyl da camb. Phenolphthaleinc. Thymol xanhd. Alizarin3. Khi xc nh ch tiu hm lng chua trong thc phm sa, ngi ta thng dng thuc th l cht g?a. Dung dch NaOH 0.1Nb. Dung dch HCl 0.1Nc. Dung dch CH3COOH 0.1Nd. Dung dch KOH 0.1N Khoa CNTP - HUFIThs. Trng Bch Chin 1733Bi tpChun 20mL dung dch HCl CxbngV(mL) dung dch NaOH 0,1N.a. Khi Cx=0,1N v V = 0 15 19,9 20 22(mL) th pH ca dung dch to thnhln lt l bao nhiu?b. Khi V = 21,2 21,8 22,2 (mL) th Cxlbao nhiu vi tin cy 95%, t = 4,3.34CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.2. Phng php th tch1.3.2.2. Chun phc chtNi dungCh thMurecid / ET.00Dung dch phn tchcha ion kim loiThuc thcomplecxonPhng phpComplecxonKhoa CNTP - HUFIThs. Trng Bch Chin 1835CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.2. Phng php th tch1.3.2.3. Chun to taNi dungCh thK2CrO4Dung dch phn tchcha ion CloThuc thdd ion AgMohr36CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.2. Phng php th tch1.3.2.4. Chun oxy ha khNi dungKhng dng ch thDung dch phn tchcha ion kim loiThuc thpermanganatKhoa CNTP - HUFIThs. Trng Bch Chin 1937CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.3. Phng php o quangNi dungMuddch muDdmuoquang38nh lut Bougher Lambere-BeerMt quang:A = . L . CA = - lg(T)Khoa CNTP - HUFIThs. Trng Bch Chin 2039Phng trnh o quang Phng trnh bt nht Phng trnh ng thng hi quytuyn tnhA = a + b.C hayC = a + b. A40CHNG 1: Cc khi nim c bn1.3. Cc phng php phn tch1.3.3. Phng php o quangp dng nh lut Beer: A = k.C vit phng trnhhi quy tuyn tnh cho dy th chun dng A = a + b.C hay C = a + b. ANi dungGiai on 1: Lp dy th chun o quangThay gi tr A (mu) vo pthqtt, tnh cnng ca dung dch mu o quangGiai on 2: Ly mu phn tch o quangKhoa CNTP - HUFIThs. Trng Bch Chin 2141CCH LP PHNG TRNHDng my tnh c nhn Tm gi tr a v b Vit phng trnh hi quy tuyn tnh Tinh nng (X) dung dch phn tch Tnh kt qu hm lng (X) trong muthc phm ban u42 Trong php phn tch hm lng st tng ctrong thc phm, kt qu mt dy chun st l:Mu 0 1 2 3 4Cppm0 0,1 0,15 0,2 0,25A 0 0,2460,3610,5120,819Phng trnh hi quy tuyn tnh c h s a v b ln lt l bao nhiu trong dng A = a + b. C ?a. 0,17v + 3,74b. 0,71v + 3,74c. + 0,17v - 3,74d. + 0,71v - 3,74 [C62]Khoa CNTP - HUFIThs. Trng Bch Chin 2243 Trong php phn tch hm lng st tng ctrong thc phm, kt qu mt dy chun st l:Mu 0 1 2 3 4Cppm0 0,1 0,15 0,2 0,25A 0 0,2460,3610,5120,819Nu mu phn tch c A = 0,672 th nng dung dch l bao nhiu?a.0.2251b. 0.1390c. 0.1374d. 0.1928 [C64]44 em 5(g) mu sa bt v c ha c 500mL dung dch mu, ri ly ra 20mL dung dch phn tch hmlng st tngKt qu mt dy chun st l:Mu 0 1 2 3 4CN0 0,1 0,15 0,2 0,25A 0 0,246 0,361 0,512 0,819Cn em 5mL dung dch phn tch khi o quang, thc A = 0,672. Hi hm lng st c trong mu ban u l bao nhiu?Khoa CNTP - HUFIThs. Trng Bch Chin 2345CHNG 2: nh lng acid v phgia1.1. nh lng acid tng1.2. nh lng benzoat1.3. nh lng sunfit46CHNG 2: nh lng acid2.1. xc nh tng chuaLy theo TCVN 1694 - 75Vi mu saNhc nh cchly mu-Ly theo can : 5% tng s-Khng di 3 can-Ly theo v tr Khng gian-Khng t hn 0.5L-L thy tinh mu, kh sch-Bo qun ni kh mt (1 thng)-L mu phi ghi r Label: -tn s hiu ngy thng ngi ly- quy cch lyCh Khoa CNTP - HUFIThs. Trng Bch Chin 2447CchTinhnhCngThctnhNguynTc chuaTng48CHNG 2: nh lng acid2.1. xc nh tng chuaNguyn tcDng dung dch NaOH 0,1N trung ha lngacid c trong mu vi cht ch th phenolphtalein1% hoc dng in cc ch th chun Khoa CNTP - HUFIThs. Trng Bch Chin 2549Mu saTi VmLCch tinhnhDung dchDung dchphntchChunbngNaOH0,1Nnh mc 100mL+ Ht VmL dung dch+ 5 git PPChun CHNG 2: nh lng acid2.1. xc nh tng chua50Nu chun bng My chun inthNhc nh-Hiu chnh li nng NaOH-Ci t cc thng s cho my inth (tham kho cch ci t Giotrnh)-Dng khuy t ng cch-Tnh kt qu trn my chun Khoa CNTP - HUFIThs. Trng Bch Chin 2651Cng thc tnhTrong : Vm: l th tch mu (mL)V: l th tch NaOH 0,1N tiu tn trong chun (mL)Vdd: th tch mu sau x l mang i chun Vdm: th tch bnh nh mcK: l h s ca loi acid d d. .. 1 0 0 0d mmK V VV V chua X (g/L) = ?52+ Vi sa kt qu biu th bng acid lactic K = 0,0090+ Vi thc phm ln men chua lactic kt qu biu thbng acid lactic K = 0,0090+ Vi dm kt qu biu th bng acid axetic K = 0,0060+ Vi cc loi hoa qu ti, siro, ko.kt qu biu thbng acid xitric K = 0,0064+ Vi du m kt qu biu th bng acid oleic K = 0,0282K l acid tng ng vi 1mL NaOH 0,1NKhoa CNTP - HUFIThs. Trng Bch Chin 2753CU HI1. Cho bit cch ly mu Bia, nc ngtkhc vi cch ly mu trong sa chuanh th no?2. Vi mu Bia, chua c tnh theo loiacid g?3. Nu cc bin php khc phc bt ctrong Bia khi phn tch cc ch tiu trongBia54CHNG 3: nh lng m, trov khong3.1. m- phng php sy3.2. Tro- phng php khi lng3.3. Khong nh lng Canxi3.4. Khong nh lng Fe3.5. Khong nh lng P3.6. Khong nh lng NaClKhoa CNTP - HUFIThs. Trng Bch Chin 2855 ngha M M T DO M VT L M HA HC56CCHTINHNHCNGTHCNGUYNTCSYKhoa CNTP - HUFIThs. Trng Bch Chin 2957CCHTINHNHCNGTHCNguynTcTROTNG58Cu hi 1. Trong Clip v cch tin hnh, s dngmu khc nhau, nn iu g thay i? 2. Phng php trong Video Clip lphng php g? 3. So snh 2 cch tin hnh, cch non gin hn/ cch no cho hiu sut tthn?Khoa CNTP - HUFIThs. Trng Bch Chin 3059CchTinhnhCngThctnhNguynTcCanxiMagie60X lmuMU LNGDng huyn ph: Lc giyDng ln men: loi b CO2Lc trn k mu bng my khuyLNGKhoa CNTP - HUFIThs. Trng Bch Chin 3161X lmuMU RNB ht v v cng cakhoang htcn v nghin mu trongmy nghin.RN62CHNG 3: nh lng m3.1. phng php SYNguyn tcMu c sy trong 5 h 870C, haohtkhi lngtrcv saukhi sylcs tnhmtng cng.Khoa CNTP - HUFIThs. Trng Bch Chin 3263Cn musaCch tinhnhMuban uMuphntchLymura -CnLy mu theoTCVN 6400Cha mu trong ctt trong t sy-Trn-SyCHNG 3: nh lng m3.1. phng php SY64Cng thc tnhTrong : m0l khi lng mu thc phmm1l khi lng ton ct rng trc khi sym2l khi lng ct v mu sau khi sy m X (g/g)100 .01 2mm m =Khoa CNTP - HUFIThs. Trng Bch Chin 3365Nguyn tcChittrotngs bngncnng, em nung n khi lng khng i. Hiuscntrcv saukhinung dng xcnhhmlngtro tng.CHNG 3: nh lng tro3.2.1. Xc nh lng tro tng66Cn muthc phmCch tinhnhMutroban uMutrophntchnguiCnmu-Khong 5(g)-Than ha nguiCn chnhxc 4 sChitLcNungCHNG 4: nh lng tro4.1. Xc nh lng tro tngKhoa CNTP - HUFIThs. Trng Bch Chin 3467Cng thc tnhTrong : m0l khi lng mu thc phmm1l khi lng chn nung rng trc khi nungm2l khi lng chn nung v mu sau khi nungRS l hm lng cht kh ca mu c xc nh theo ISO 1572, c tnh bng phn trm khi lngTro tng X (g/g)100 ..01 2RS mm m =68Nguyn tc tm CanxiMu mui c ho tan trong acid HCl. Hmlng canxi ho tan trong mu mui cxc nh bng phng php khi lng. Tinhnh ta caxi di dng CaC2O4, lc, ra vnung ta nhit 9500C n khi lngkhng i. T tnh c hm lng caxic trong mu. Khoa CNTP - HUFIThs. Trng Bch Chin 3569Nguyn tc tm MagieDch lc sau khi xc nh canxi s c sdng xc nh Mg bng phng php khilng. Ta Mg di dng MgNH4PO4.6H2O v nung ta nhit 8500C trong 2 gin khi lng khng i chuyn v dngcn Mg2P2O7. T , tnh c hm lngmagi c trong mu mui. 70Mu Muim(g)Xc nhCanxiDung dchDung dchphntchNungtaCnlngrn m1+ c x l+ Ha tan bng HCl+ Lc+ Ht 50mL dch lc 1+ cht th+ Lc gn, dch loc 2 v taKhoa CNTP - HUFIThs. Trng Bch Chin 3671Dch lc 2 ca CanxiXc nhMagieDung dchTaNungtaCnlngrn m2+ c x l+ un nng+ Lc72Cng thc tnh100 7143 . 0 %01 2 =hbdmVVmm mCa100 2184 . 0'%01 3 =hbdmVVmm mMgKhoa CNTP - HUFIThs. Trng Bch Chin 3773CchTinhnhCngThctnhNguynTcSt74Nguyn tcDa vo kh nng to phc mu gia Fe2+v 1 10phenanthrolein.Trong khongpH=2 9,phc mu camgia Fe2+v 1 10 phenanthrolein hp thu cc i bc sng 505nm.Bng cch xy dngng chun A f(C),ta c th xc nhhm lng Fe trong muKhoa CNTP - HUFIThs. Trng Bch Chin 3875Cch tinhnhTo dy chun o quangChun b mu BiaT pthhtt tnh nng Fe76To dy chuninh mc thnh 100mL Nc ct2 mL V (o phenanthrolein)25mL Acid ascorbic (mg)3 2 1 0.5 0.25 0 V Fe2+(mL) (0.1 mg/mL)5 4 3 2 1 0 STTKhoa CNTP - HUFIThs. Trng Bch Chin 3977Chun b mu Biainh mc thnh100mL Nc ct2 mL V (o phenanthrolein)25mL Acid ascorbic (mg)10 10 10 Mu Bia (mL)3 2 1 STT78Tin hnh o quangVit phng trnh hi quy tuyn tnhTnh hm lng FeKhoa CNTP - HUFIThs. Trng Bch Chin 4079Cng thc tnh hm lng stmbdmFeVVC C =80CchTinhnhCngThctnhNguynTcPhotphoKhoa CNTP - HUFIThs. Trng Bch Chin 4181CchTinhnhCngThctnhNguynTcNaCl82CHNG 4phn tch hm lng CarbonHydrat4.1. nh lng ng kh4.2. nh lng ng tng4.3. nh lng tinh bt4.4. nh lng Dextrin4.5. nh lng PectinKhoa CNTP - HUFIThs. Trng Bch Chin 4283CHNG 4: phn tch hm lngCarbonHydrat4.1. nh lng ng kh4.1.1. Phng php Bettrand4.1.2. Phng php Lane Eynon4.1.3. Phng php DNS84Mc tiu tit hc 1. trnh by c nguyn tc, cch tinhnh phn tch ung kh bng phngphp Bettrand 2. phn tch c cc yu cu k thut vcch khc phc cc vn ny sinh trongqu trnh tin hnh 3. trnh by c cng thc v p dngphp tnh cng thc vo trong cc bi tp, tr li cc cu hi trong biKhoa CNTP - HUFIThs. Trng Bch Chin 4385Sa l mt loi thc phm c gi tr dinhdng cao,cung cp ngun Protein,ngunnng lng cho c th. Sa cn c kh nngcung cp cho c th ngun Calci rt tt choxng, nht l ngi gi v tr em. T sa cth ch bin nhiu sn phm khc:phomt, b, sa chuaGii thiu: Sa v cc ch phm tsa86Thnh phn trong mt s sa0,8 4,5 7,9 5,8 Sa cu0,8 4,7 4,1 3,6 Sa d0,7 4,8 7,5 4,0 Sa tru0,7 4,8 3,7 3,5 Sa b0,5 6,2 1,7 2,2 Sa nga0,2 7,0 3,8 1,2 Sa mkhong Carbon hydrateChtboProteinLoi sa(g/L)Khoa CNTP - HUFIThs. Trng Bch Chin 44Trong ngnh ha thc phm v dinh dng, ngi ta chia dngthnh 3 dang: dng dn gin, dng di (gm 2 gc dng dngin kt hp vi nhau)v dng da (gm nhiu dng dn kthp lai).Trong sa c dng lactose M = 342.3 (cn gi l dng sa v ctrong sa ngi v dng vt).y cng l dng di,khi thyphn s cho 2 gc dng dn l glucose v galactose.Lactosekt tinh chm,tinh th cng v c nhiu dang tinh th. Vitamin B2c th c ch s kt tinh ca lactose. ngt ca cc loai dng khng ging nhau, nu ly vj ngt cadng saccarose lm chun (100) th lactose 16. Lactose (C12H22O11) - mt loi ngc bit+galactoseglucoseNi thmCTCT ca glucose v galactosetheo dng vng - dng thngC5H11O5-CHO + 2 Cu(OH)2C5H11O5-COOH + Cu2O + 2H2OKhoa CNTP - HUFIThs. Trng Bch Chin 45ng khng lactosevncn tnh kh v vncn gc OH glucozit t dong kh l g?ng kh l ng c tnh kh nh vonhm andehyt hoc ceton (i vi mchthng)v gc OHglucozit t do(i vimch vng)Khoa CNTP - HUFIThs. Trng Bch Chin 4691NI DUNG PHP PHN TCHPhng php BETTRANDNGUYN TCCCH TIN HNHCCH TNH KT QUMT S VN QUAN TM92NguyntcPhng php BettrandTrong mi trng kim mnh,glucid trctip kh oxykhi c mt cht kh lCu(OH)2to thnh kt ta di dng Cu2O mu gch.Lng Cu2Os phnngvi mt lng d Fe3+ sinh ra mtlng Fe2+tng ng v c chun bng dungdch KMnO4trong mi trngacid. Khoa CNTP - HUFIThs. Trng Bch Chin 4793K thutphntchPhng php BettrandDng phng php chun Oxyha kh - Phng phpPermanganatPhng trnh phn ng minh ha :RCHO +2Cu(OH)2RCOOH + Cu2O +2H2O.Cu2O + Fe2(SO4)3+ H2SO42CuSO4+ H2O + 2FeSO410FeSO4+ 8H2SO4 + 2 KMnO4K2SO4+ 2MnSO4+ 5Fe2(SO4)3 + H2O94Mu sa10(g)Cch tinhnhDung dch+Nc nng+5mL K4[Fe(CN)6] 15%+5 mL (CH3COO)2Pb 10%Dung dchban u+ ngui,+nh mc thnh 100mLDung dchmuLc1. Giai on khtpKhoa CNTP - HUFIThs. Trng Bch Chin 4895Dung dchMuDd mu muxanh c tamu gch +Fehling A+Fehling B+un siDung dchmu+Lc, Ra ta+ Fe2(SO4)35%ChunbngKMNO40.1N +H2SO46NCch tinhnh2. Giai on xcnh ng kh96Khi dung dch chuyn sang mu hng nht1. Mu hng nht l mu cach th no?2. Khi dung dch chuynsang mu hng n nh, thim dng ny trc hay sau im tng ng?Khoa CNTP - HUFIThs. Trng Bch Chin 4997CU HI TNG T1. Trong cc cu sau, cu no ng nht:a. im cui chun l im m ti cht ch th i mub. im tng ng l im m ti cht ch th i muc. im tng ng thng thng l im cui chun d. im dng chun cng l im tng ng2. im dng chun l g?a. L im tng ng ca php chun b. L thi im lc dung dch chuyn mu theo cht ch thc. L thi im php chun c sai s nh nhtd. L im m cc cht phn ng va 98CCH TNH KT QUBc 1:T th tch chun ca KMnO4,-tra bng Bettrand, c c khi lngng tng ngBng BettrandKhoa CNTP - HUFIThs. Trng Bch Chin 5099CCH TNH KT QUBc 2: Vi th tch KMnO4khng c trong ct, thdng phng trnh hi quy tuyn tnh, xc nh khi lng ng khPhng trnh hiquy tuyn tnh100CCH TNH KT QUBc 3: Thay gi tr khi lng ng kh tm cvo cng thc tnh hm lng ng khtrong mu saCng thc xcnh hm lngKhoa CNTP - HUFIThs. Trng Bch Chin 511013 cu hi sau khi xem Clip 1. im khc bit g trong giai on khtp? 2. v sao phi un nh dung dchFehling? 3. tip theo sau on lc bng chnkhng, cn phi lm g Cu2O gimtip xc vi khng kh, nhm trnh smt lng Cu2O ?102Bi tp Cu hi v nh1. Khi phn tch hm lng ng kh c trong thc phm sa bng phng php Bertrand, ngi ta dng chun khi no?a.khi dung dch mu phn tch chuyn sang mu hng tmb. khi dung dch mu phn tch t ti im tng ngc. khi dung dch mu phn tch c n nhd. khi th tch dung dch thuc th t ti u [C73]2. Khi phn tch hm lng ng kh c trong thc phm sa bng phng php Bertrand, khi nim ng khl g?a.l glucoz vn cn cha nhm -CHOb. l ng b kh hon tonc. l glucoz khng th thc hin phn ng oxy ha khd. l Glucoz dng Disaccarit [C74]Khoa CNTP - HUFIThs. Trng Bch Chin 52103Bi tp Cu hi v nh3. Khi phn tch hm lng ng kh c trong thc phm sa bng phng php Bertrand, ngi ta cho thuc thFehling A v B vo lm g?a. oxy ha hon ton lng ng khb. kh hon ton lng ng khc. oxy ha hon ton lng ngd. to mt mi trng n nh [C75]4. Khi phn tch hm lng ng kh c trong thc phm sa bng phng php Bertrand, ngi ta cho thuc thFehlingAv Bvoth dungdchmuphntchs cmu g?a.mu xanh bicb. mu vng rmc. mu hng nht d. mu tm [C76]104BNG TRA KHI LNG NG KH95 26.7 64 18.8 33 10.194 26.5 63 18.6 32 9.8893 26.2 62 18.3 31 9.5892 26 61 18 30 9.391 25.7 60 17.7 29 8.9790 25.5 59 17.5 28 8.789 25.2 58 17.2 27 8.3988 25 57 16.9 26 8.0987 24.7 56 16.6 25 7.8186 24.5 55 16.4 24 7.5285 24.2 54 16.1 23 7.3884 24 53 15.9 22 6.9183 23.8 52 15.5 21 6.61Khi lngglucose (mg)Th tchKMnO40.1NKhi lngglucose (mg)Th tchKMnO40.1NKhi lngglucose (mg)Th tchKMnO40.1NKhoa CNTP - HUFIThs. Trng Bch Chin 53105 th biu din mi lin h gia khi lng ng glucose (mg) v th tch KMnO4 0.1Ny = 3.6365x - 3.5569R2 = 0.99890204060801001200 10 20 30Th tch KMnO4 0.1N (ml)Khi lng glucose (mg)106Cng thc tnh cho TP rnTrong : G: khi lng ng nghch chuyn hoc ng glucose (g) tng ng vi s mL KMnO40,1N trong bng. m: khi lng mu thc phm (g)Vdd: th tch dung dch mu c ht ra tin hnh phnng v chun .Vm: th tch bnh nh mc (th tch dung dch mu sau khix l)F: h s hiu chnh nng KMnO4. (Vi dung dch hiuchun, F = 1) m VF V GXddm.100 . . . 10 .(%)3 =Khoa CNTP - HUFIThs. Trng Bch Chin 54107Cng thc tnh cho TP lngTrong :G: khi lng ng nghch chuyn hoc ng glucose (g) tng ng vi s mL KMnO40,1N trong bng.Vm: th tch mu sau ban uVdd: th tch dung dch mu c ht ra tin hnh phnng v chun .Vm: th tch bnh nh mc (th tch dung dch mu sau khix l)F: h s hiu chnh nng KMnO4. Vi dung dch hiuchun, F =1 FV VV Gl g Xm ddm...) / ( =108CHNG 4: phn tch hm lng CarbonHydrat4.2. nh lng tinh bt4.2.1. c im phn tch4.2.2. TCVN 5535 : 1991 TCVN 3294-80Khoa CNTP - HUFIThs. Trng Bch Chin 55109c im tinh btAmoloseAmilopectin110Amilose chim 10 20%, tan trong nc Tc dng vi iod cho mu xanh c trngKhoa CNTP - HUFIThs. Trng Bch Chin 56111Amilopectin chim 80 90 %, khng tan trong nc Tc dng vi iod cho mu nho112Phn ng thy phn To glucozeKhoa CNTP - HUFIThs. Trng Bch Chin 571134.2.2. TCVN 5535 : 1991 Nguyn tciu kin Tin hnh114Nguyn tc da trn nguyn l nghch chuyn ngt phnng thu phn hon ton capolysacarit bng cc axit long,nhngkhng nh hng n lactoza v cc loing khc. khng lm thayi lng lactoza bngcch x l vi amoniac,sau lm trungho NH3d,ri cho thm dungdch kmaxetat v kali feroxyanuaKhoa CNTP - HUFIThs. Trng Bch Chin 58115Tin hnhChun b muTnh kt quTin hnh phng phpth116Chun b muSa cSa btSatiKhoa CNTP - HUFIThs. Trng Bch Chin 59117Mu sa40(g)Cch tinhnhDung dch+Nc nng dn t 50mL n 150mL(un nh n 900C, ngui n t0phng)+5mL NH32N 10 pht+12,5mL K4[Fe(CN)6] 1N+12,5 mL (CH3COO)2Zn 2NDung dchban u+ un nng nh+ ngui trong 10 phtDung dchPhncc(D)Lc1. To dung dchphn cc118Dung dchPhn cc (D)40mLun Dd 600C+6mL HCl(trong BDM 50mL)Dung dchPhn cc(J)+ bp cch thy+15 phtCch tinhnhXc nh ngnghch chuynKhoa CNTP - HUFIThs. Trng Bch Chin 60119Tnh kt quD: S phn cc k dung dch (D)J: S phn cc k dung dch (J)Q: h s nghch chuyn (tra bng)V: Th tch mu ban u trc khi phalongm: khi lng mu ban ui: chiu di ng phn cc kv: s hiu chnh th tchm i vv VQJ D.1 4 / 51204.2.2. Tiu chunTCVN 3294-80 Nguyn tcTin hnh iu kinKhoa CNTP - HUFIThs. Trng Bch Chin 61121Nguyn tc Lc mu sau khi ha tan mu bng Cn ete. Ha tan cn lc bng dung dch HClc, ri to ta bng cn 960. Lng ta trc v sau khi dng HCl lc s tnh hm lng tinh bt trongmu ban u122Mu sa2(g)Cch tinhnhCnRa bng Cn Ete trongphu lc chn khngDung dchnhmc100mL+11mL nc ct+14mL HCl cLclyrn+Ht 50mL+110mL cn 960+12h trong tlnh1. To dung dchphn ccKhoa CNTP - HUFIThs. Trng Bch Chin 62123Tnh kt quHm lng tinh bt c tnh trong 100g mu% X = (m1 m2 ).100124CHNG 5phn tch hm lng Protid5.1. nh lng N tng bngphng php Kjendalh5.2. nh lng N acid amin5.3. nh lng m thiKhoa CNTP - HUFIThs. Trng Bch Chin 63125NI DUNG PHP PHN TCHPhng php KJENDALHNGUYN TCCCH TIN HNHCCH TNH KT QUMT S VN QUAN TM126NguyntcPhng php KjendalCht hu cc phnhy bng acidsulfuricvi scmt ca cht xc tc . sn phm phn ng c kimha ; sauoc chng ct vachun lngamoniac gii phng .tnh hm lng nitovanhn ktquvi hsquic thuc hm lng protein thKhoa CNTP - HUFIThs. Trng Bch Chin 64127Mu sam(g)Cch tinhnhDung dchDung dchsi haxanhDung dchphntch+a vo bnh Kjendal+Hhp xc tc+H2SO4c+un nng 3000CNgui1. Chun b mu128Dung dchPhn tchChng ctDung dchchunH thng KjendalDung dch imuChun b bnh hp thu+dung dch hp thu+Ch thCch tinhnh2. Chng ctKhoa CNTP - HUFIThs. Trng Bch Chin 65129Cng thc xc djnh hmlng Nit% NitTrong :V1,V2:l th tch H2SO4chun mu tht vmu trng (ml)m: khi lng mu (g)1 20,028.(V ) =Vm130CHNG 6phn tch hm lng Lipic6.1. i cng v cht bo6.2. Ch s acid6.3. Ch s peroxid6.4. Ch s Iod6.5. Ch s x phng haKhoa CNTP - HUFIThs. Trng Bch Chin 66131 NGHA - LIPITLipit ngha l hp cht bo, v l hp cht hu c a chc, gm nhng cht nh du n, m .... L tnh : Chng c nht cao, khng tan trong nc, tan trong cc dung mi hu c nh ether, chlorphorm, benzene, ru nng . Hai nhm lipid quan trng i vi sinh vt l: nhm c nhn glycerol v nhm sterol. 132 NGHA - LIPITTc dng:a) Ngn nga x va ng mch bng cch kt hp vi cholesterol to cc ester c ng, khng bn vng v d bi xut ra khi c th.b) iu ha tnh bn vng ca thnh mch: nng cao tnh n hi v h thp tnh thm ca thnh mch.c) C lin quan n c ch chng ung th.d) Cn thit cho cc chuyn ho cc vitamin nhm B.e) Mt s t chc nh: gan, no, tim, cc tuyn sinh dc c nhu cu cao v cc acid bo cha no, nn khi khng c cung cp t thc n th cc ri lon s xut hin cc c quan ny trc tin.Khoa CNTP - HUFIThs. Trng Bch Chin 67133 NGHA - LIPITf) l thnh phn thit yu ca t bo, ca cc mng c th v c vai tr iu ha sinh hc cao. No b vcc m thn kinh c bit cn giu cht bo. h) Cht bo cung cp cc acid bo thit yu khng no a ni i, chui di l tin cht ca mt lot cc cht c hot tnh sinh hc cao nh prostaglandin, leukotrienes, thromboxanesCc eicosanoids ny l cc cht iu ha rt mnh 1 s t bo v chc nng nh: kt dnh tiu cu, co mch, ng ng ng mch Botallii). Trong c th cht bo l ngun d tr nng lng ln nht.134TinhnhCngThcNguynTcCh sacidKhoa CNTP - HUFIThs. Trng Bch Chin 68135TinhnhCngThcNguynTcCh speroxyt136TinhnhCngThcNguynTcCh sx phngKhoa CNTP - HUFIThs. Trng Bch Chin 69137TinhnhCngThcNguynTcCh sIod