Bacteria Resistance to Antibiotics

Effect of Temperature and Ampicillin on Escherichia coliBecca Goslow- WMHS Frosilda Pushani- SHHS 1Did You Know?

2http://thescienceofacne.com/amoxicillin/https://harveymillican.wordpress.com/tag/amoxicillin/Goslow-PushaniWatch This!

3https://www.youtube.com/watch?v=RpKZvnJwicAGoslow-Pushani3Terms to knowAntibiotic ResistanceEscherichia coliAmpicillinInhibition Zone

Goslow-Pushani4What is Antibiotic Resistance?Antibiotics are no longer an option.Bacteria takes over and becomes immune.Goslow-Pushani5

http://www.medicinenet.com/antibiotic_resistance/page4.htmEscherichia coli6

http://www.britannica.com/blogs/2011/06/evil-coli/http://www.health32.com/e-coli-symptoms-and-treatment/Goslow-Pushanihttp://www.britannica.com/blogs/2011/06/evil-coli/http://www.health32.com/e-coli-symptoms-and-treatment/6Ampicillin7

www.newlystar-medtech.com/Ampicillin-Capsules-12.htmlhttp://www.drugs.com/cdi/ampicillin.htmlGoslow-Pushanihttp://www.newlystar-medtech.com/Ampicillin-Capsules-12.htmlhttp://www.drugs.com/cdi/ampicillin.html7Inhibition Zones8

http://web.med.unsw.edu.au/cdstest/gtf_cds_site/webpages/plates/plate1a.htmGoslow-Pushanihttp://web.med.unsw.edu.au/cdstest/gtf_cds_site/webpages/plates/plate1a.htm8OverviewProblem/Hypothesis.Materials/Procedure.Results/Statistics.Accept/Reject Hypothesis.Possible Improvements.

9Goslow-PushaniProblemThe effect of antibiotics on the growth of bacteria throughout generations.

10Goslow-PushaniHypothesisIf Ampicillin is used, then the Escherichia coli will develop a resistance to the antibiotic. 11Goslow-PushaniMaterials12

Blank DisksAmpicillinSterile SwabsPetri DishesStarter Plate (E. coli)Bunsen BurnerAnalytic BalanceIncubatorsGoslow-PushaniValuesGoslow-Pushani13

Procedure14

https://www.youtube.com/watch?v=cneascR3OEcGoslow-Pushanihttps://www.youtube.com/watch?v=cneascR3OEc14Procedure15

https://www.youtube.com/watch?v=bRadiLXkqoUGoslow-Pushanihttps://www.youtube.com/watch?v=bRadiLXkqoU15Procedure16

http://en.wikipedia.org/wiki/Capsule_(pharmacy)

http://www.carolina.com/microbiology-supplies/antibiotic-sensitivity-disks-blank-sterile-vial-50/805091.prGoslow-Pushanihttps://www.youtube.com/watch?v=cneascR3OEc16Procedure17

http://out-grow.com/cultivation-equipment-c-1/

http://www.ufz.de/iwas-sachsen/index.php?en=21853Goslow-Pushani17Procedure18

Goslow-PushaniProcedure

(A+B+C) 3 = Size of an inhibition zone.19Goslow-PushaniResistanceE. coli.Ampicillin.Zone of Inhibition/ Resistance.

20Goslow-Pushani20Results21

Goslow-PushaniObservationsDateObservation3/11/14One Petri dish had a smaller inhibition zone from the rest. TheE. coli was smelly and white.3/13/14The Petri dishes had a lot of E. coli, the bacteria was white and extremely smelly.3/14/14The E. coli on some of the Petri dishes were blotchy.3/18/14The E. coli was smellier than usual and filled up every Petri dish completely, besides the zone of inhibition.22Goslow-PushaniEffect of Ampicillin23

Goslow-PushaniEffect of Incubation Temperature24

Goslow-PushaniInteraction Effect25

Goslow-PushaniOutliers?26

Goslow-PushaniAccepted Hypothesis!

27Goslow-Pushani28Goslow-PushaniAccepted Hypothesis!RecapWhat we planned.What happened.Added Ampicillin to E. coli and incubated the petri dishes.The bacteria and inhibition zones grew.Overtime, the inhibitions decreased in size. The hypothesis was accepted!

29Goslow-PushaniImprovements for the FutureRun more trials.Have a wider range of the predictor variables.Use of an actual human. Stop experimental flaws.30Goslow-Pushani30Flaw and Human ErrorsOff temperatures.Off measurements of Ampicillin.Time issues.

Goslow-Pushani31Who does this effect?Chemists/ScientistsDoctorsYOU!Goslow-Pushani32Save Your LifeDo not take the antibiotics for viral infections.Take an antibiotic exactly as a healthcare provider tell you.Keep antibiotics to a minimum.33Goslow-PushaniAcknowledgementsMr. AcreMr. EstapaMrs. GravelVeronica & Abigail Goslow-Pushani34Works Cited35"Ampicillin Capsules." Ampicillin Capsules. Web. 28 May 2014.

"Ampicillin Capsules: Indications, Side Effects, Warnings - Drugs.com." Ampicillin Capsules: Indications, Side Effects, Warnings - Drugs.com. Web. 27 May 2014.

"Antibiotic Resistance." Antibiotic Resistance. University of Utah Health Sciences, n.d. Web. 14 May 2014. "Antibiotic Resistance a Serious Threat." The Australian Government, n.d. Web. 10 May 2014. "Antibiotic Resistance Questions & Answers." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 18 Dec. 2013. Web. 10 May 2014.

"Antibiotic Sensitivity Disks, Blank, Sterile, Vial 50." Science Supplies & Curriculum. Web. 28 May 2014.

"Aseptic Technique." YouTube. YouTube. Web. 28 May 2014.

"Capsule (pharmacy)." Wikipedia. Wikimedia Foundation, 24 May 2014. Web. 28 May 2014.

"Cultivation Equipment." : Out-Grow, Mushroom Growing Supplies & Quality Service. Web. 28 May 2014.

Dzidic, Senka. "Antibiotic Resistance Mechanisms in Bacteria: Biochemical and Genetic Aspects." Food Technology and Biotechnology. N.p., n.d. Web. 15 Mar. 2014. Goslow-PushaniWorks Cited"E.coli Symptoms and Treatment." Health32Com Ecoli Symptoms and Treatment Comments.Web. 28 May 2014.

"Evil E. Coli." Encyclopedia Britannica Online. Encyclopedia Britannica. Web. 28 May 2014.

"HowStuffWorks. "How Do Antibiotics Work?""HowStuffWorks. N.p., n.d. Web. 26 May 2014."International Water Research Alliance Saxony." Running Waters, Microbiology -. Web. 28 May 2014.

"Media Prep." YouTube. YouTube. Web. 28 May 2014.

Paphitou, Niki I. "Antimicrobial Resistance: Action to Combat the Rising Microbial Challenges. International Journal of Antimicrobial Agents. N.p., n.d. Web. 15 Mar. 2014.

"Plate1A." Plate1A. Web. 27 May 2014."Science Fair Projects - Bacteria resistance to antibiotics." Science Fair Projects - Bacteria resistance to antibiotics. All Science Fair Projects, n.d. Web. 14 May 2014.

"The Science of Acne." The Science of Acne. Web. 28 May 2014.

"Tag Archives: Amoxicillin." Harvey Millican Raising Your Kids Without Lowering Your IQ. Web. 28 May 2014.

"The Threat of Antibiotic Resistance." YouTube. YouTube. Web. 28 May 2014.

Todar, Kenneth . "Bacterial Resistance to Antibiotics." Bacterial Resistance to Antibiotics. N.p., n.d. Web. 14 May 2014.

36Goslow-Pushani36Any Questions?37Goslow-Pushani38Goslow-Pushani