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BENCHTOP FERMENTOR & BIOREACTOR SYSTEMS

A SELECT NEW RANGE OF VERSATILE, HIGH PERFORMANCE 3 - 20L AUTOCLAVABLE SYSTEMS WITH EASY-TO-USE TOUCHSCREEN CONTROLS

www.cleaverscientifi c.comwww.cleaverscientifi c.com

Expertise And Excellence In Electrophoresis

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Cleaver Scientifi c is proud to launch an exciting new range of high performance fermentors

and bioreactors.

Remarkably user-friendly and equipped with intuitive controls, these fermentors and bioreactors are suitable for most fermentation and cell culture applications performed by:

• University teaching labs;

• Life science research labs;

• R&D facilities within biofuel and biotech companies;

• Pharmaceutical organisations;

• Food and beverage manufacturers.

Each system is supplied as a customised solution with vessel, impeller, control station and probes for growth of diff erent microbes, yeast, mammalian, insect and plant cells, removing the complexities often faced with other brands of equipment selection and set up.

Assembly is straight forward with the unit being unpacked and made ready for the autoclave in less than an hour.

The control station is fully integrated with built-in peristaltic pumps, gas fl ow control, and pH, dissolved oxygen (DO), substrate feed and antifoam functionalities all installed in one bench top unit.

An easy-to-use large 10.4-inch colour touchscreen interface allows setting and real-time pictorial display of agitation speed, temperature, pH and dissolved oxygen (DO), while 4 assignable built-in peristaltic pumps act in tandem with the corresponding probe sensor to control pH, DO and antifoam feeds.

Each probe is connected to an easily accessible side panel that also houses quick-fi t connectors for rapid set up of the heating system and ancillary equipment for aeration and cooling.

...Introducing a new player in bench top fermentors and bioreactors !


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Changing with your needs

Grow almost any cell type − With a wide range of vessels and impellers, including

customised options, Cleaver Scientifi c fermentation and bioreactor systems have the

capacity to grow virtually any cell type: whether it’s anaerobic or aerobic microbes, yeast,

insect, plant or mammalian cells.

Pre-programmed for fermentation & cell culture − All Cleaver Scientifi c fermentors

and reactors are pre-programmed with fermentation and cell culture operating modes

allowing each system to adapt as your needs change.

Diff erent vessel confi gurations − All fi ve types of interchangeable single-wall,

double-wall, heat-blanketed, jacketed and air lifter vessels are available in 3 to 20 litre

volumes and made from autoclavable borosilicate.

Multiple options & accessories − Full adaptability is assured with up to 4 probe

options for pH, DO and temperature; 4 brushless motor drives with 12 choices of impeller;

2 adjustable fl ow meters, and 5 vessel types in as many as 6 diff erent volumes.

Simple to use

Touch-and-grow − A large 10.4” colour touch screen interface built within the control

station allows the user to control and view each parameter within a conspicuous, easy-to-

read pictorial display.

Helpful screen tabs − The colour touch screen is pre-confi gured for use with the

selected reactor vessel, while 8 diff erent screen TABS facilitate user-fi endly control of all

critical process values within a single screen (see page 5).

Same software on all units − The same control software features on all fermentors

and bioreactors allowing the user to change to other Cleaver Scientifi c systems when

required, while ethernet remote control is available without additional software.

upgrades.

Automatic Controls

Gas fl ow control − Automatic multi-gas mixing options - up to two and four

respectively in fermentation and cell culture mode - ensure optmised yields and cell

growth, while a selection of customisable gas fl ow options provide complete fl exibility by

allowing customers to design systems specifi c to their needs.

Adjustable air fl ow − Each system features as standard an adjustable air fl ow

rotameter on the front of the control station. An optional oxygen (O2) enrichment module

comprising adjustable rotameter and O2 enrichment valve may also be fi tted. Bespoke

thermal mass fl ow control (TMFC) options are available on request.

2-stage cascade with P-I-D technology − A built-in 2-stage ‘cascade’ feature utilises

P-I-D (Proportional-Integral-Derivative) control technology to maintain the DO setpoint

through automatic adjustment of agitation speed and substrate feeds, and optional O2

enrichment. The adjustable P-I-D values for pH and DO may be defi ned automatically by

the size of the reactor vessel, or after manual fi ne-tuning by the user.

Leave it to its own devices − P-I-D control of each setpoint obviates the need for

constant operator supervision, thus aff ording the user the time to perform other tasks at

their convenience while the system remains unattended. Choose glass-walled vessels as shown or heat-blanketed vessels. Customise your system with up to two rotameters or optional TMFC for gas overlay and sparge.

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A wide range of accessories, including multiple probe options, ensure easy customisation of each bench top fermentation and cell culture system for many diff erent cell types and microbes. From left to right, probes include: (1.) pH, (2.) DO, (3.) temperature, (4.) antifoam and (5.) oxidoreduction potential (ORP).

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A low maintenance, brushless 24V DC motor (1.) is avail-able as standard with each system and may be used with both types of standard impeller, such as the Rushton-style, as shown (2.), for fermentation, or the low-shear pitched blade impeller (3.) for more fragile insect, plant and ani-mal cultures. Bespoke impellers are available on request for full fl exibility.

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Heat-blanketed dish bottom vessels − available in 6 sizes, ranging from 3 to 20L. Larger 15L and 20L vessels are ideal for process scale-up as cost-eff ective

alternatives to expensive sterilisable-in-place systems (SIP). Dry-heating blanket suitable for growth of microbes and non-photosynthetic plant cells.

Single-wall plain bottom vessel with heating base − available in 4 sizes: 3, 5, 7.5 and 10L. Dry-heating base ensures rapid heating and cooling. Perfect entry-level system for microbial fermentation.

Low maintenance 30-1200rpm brushless motor − available for all models. Supplied with 'quick' connector for easy vessel connection. Optional low 5-300rpm motor recommended for cell culture.

Side panel − provides convenient access to all connections for probe sensors and motor, as well as the sparger, cooling system and condenser.

10.4-inch colour touchscreen interface − included as standard with all units. Helpful screen tabs guide you through each step of the set-up process. 'Get touching and start growing!'

Double-wall round bottom vessels − available in 4 sizes: 3, 5, 7.5 and 10L. Warm or cool water circulates through the outer wall cavity for thermostatic temperature control. Especially suitable for growth of mammalian, microbial and insect cells.

Four peristaltic pumps − may be linked directly to acid, base, antifoam and substrate feeds. Pump speed adjustable within 1rpm increments.

Up to two rotameters − one for gas fl ow control with optional second rotameter for O2 enrichment.Stop-Start −convenient front access.Small footprint − 40x50cm (WxD) - allows control station to fi t most benches comfortably.

Air-lifter vessels − available in double-jacketed (1) and single-wall (2) formats - facilitate unique impeller-free growth of fragile plant and fungal cells using micro-sparger and adjustable draft tube.

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Sensor panel − with connection ports for pH, ORP, DO, antifoam and vessel temperature probes; gas mixing station and motor; and dry-heating base unit / blanket and blanket temperature probe (on CSL FS-02 models only).

Air and cooling panel − with connection ports for condenser cooling and vessel cooling-coil water inlets and outlets; condenser fl ow regulator; main water inlet and outlet drain; gas outlet to sparger; and air and O2 inlets.

USB port − for data export to USB fl ash drive or computer.

Remote control and system expansion panel − includes extension ports, RE-485 connection for ancillary fi fth peristaltic pump, power socket and ethernet port for connection to optional laptop shown.

'Get touching and start growing!'

Control Station and Vessels

Side Panel

Simple to use touchscreen interface facilitates set up in less than an hour. No confi guration needed.

ilable in double


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Threaded penetrations in 316L stainless steel headplate − provided for probes; acid base and substrate feeding ports; septum port for small volume injection (e.g. antibiotics); condenser to prevent culture media evaporation; sparger, cooling ports, and inoculation and sampling ports. T-shaped hand-lifters − for convenient vessel transfer and manoeuvrability.

Sample Control Screens

1. MONITOR - shows a full summary of all critical processes within one easy-to-read screen, including: operation status; setpoint and present value of each parameter; user login and working status of antifoam, pH and substrate feeds; process time and manual or automatic operation.

2. PUMP - for simultaneous control, calibration and assignment of all 4 peristaltic pumps, plus fi fth ancillary pump if used.

3. CONTROL - performs manual or automatic control of pH, agitation, temperature and DO setpoints.

4. SETTING - allows selection of the program schedule for each automatic process.

5. CHART - shows real time recording of trend data for each fermentation or cell culture process

6. CALIBRATION - for accurate calibration of each probe sensor.

7. MAINTENANCE - to set up a service and maintenance schedule.

6. CALIBRATIONTIO - for accurate calibration oftion o

7. MAINTENANCE -ANCE -7. MAINTENA to set up a service anda service andto set up a

External peristaltic pumps − may be connected to control station by RE-485 port. Fifth external pump may be controlled in the PUMP screen tab (see sample screen 2 below). Pump head is reversible and may be used with any one of six silicon tubes, ranging from 0.8 to 7.9mm in internal diameter, depending on the desired fl ow rate (36 to 2300 mL/min). Silicon tubes supplied in 7.6m lengths.

Vessel Headplate

Ancillary Pump

Each screen may be viewed and programmed using ethernet remote control with optional lap top.

8. HELP - for set up and operation assistance, particularly the cooling and air system following sterilisation.

Single-wall round bottom vessels − available in 4 sizes: 3, 5, 7.5 and 10L. Inner circulation loop facilitates rapid heating and cooling. Ideal for anaerobic and aerobobic fermentation.


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TECHNICAL SPECIFICATION*

Th ermostatic Bioreactor and Fermentation Systems (CSLFS-01)Double-wall round bottom vessel systems Single-wall round bottom vessel systems Air-lifter vessel systems

Double-wall Single-wall

VESSEL

Series CSLFS-V-A CSLFS-V-B CSLFS-V-C

Working volume 3L 5L 7L 10L 3L 5L 7L 10L 5L

Total volume 3.7L 6.8L 8.9L 12.5L 3.7L 6.8L 8.9L 12.5L 6.9L

Design All vessels comprise autoclavable borosilicate glass contruction with 316L stainless steel headplate with fi ttings

CONTROL

STATION

Design features(A) Compact control station with integrated controller including built-in pumps and side panel capable of supporting utilities; (B) storage for 59,994 process programmes; (C) log data storage for 10 process monitoring fi les; (D) real-time trend data recording; (E) industrial standard ABS front panel with painted iron housing

Display 10.4-inch (265mm) colour touchscreen interface supplied as standard with each control station

Function Fermentation and cell culture monitoring and control

Dimensions Footprint (W x D): 400 x 500mm (15.75 x 19.69"); Height: 740mm (29.13")

TEMPERATURE

Range 5˚C above coolant to maximum 60˚C

Control

(A) Thermostatic system with programmable 15-step PID control; (B) built-in heat exchanger (400W heater / water circulation pump); (C) automatic cooling water valve

Double-wall water jacket heater and circulation loop (see B and C above)

Single-wall vessel with inner coil heater and circulation loop (see B and C above)

Double-wall water jacket heater and circulation loop (see B and C above); no temperature control for single-wall vessels

Probe Platinum RTD probe (Pt100)

AGITATION

Drive Removable top 'brushless' motor n/a

Range 30-1200rpm standard motor for routine fermentation and cell culture; 5-300rpm option for shear sensitive cell lines n/a

Control Manual setting or programmable 15-step PID control n/a

Impellers2 pieces of adjustable pitched-blade impeller for fragile cell types

3 pieces of adjustable Rushton-style impeller for less shear-sensitive microbial cell types

n/a

Baffl es Removable 316L stainless steel baffl e for fermentation n/a

AERATION

Gas fl ow optionsStandard built-in air-fl ow rotameter (1-10Lpm for 3/5L or 2-20Lpm for 7/10L vessels); optional O2 enrichment module with rotameter and enrichment valve (bespoke TMFC possible - please enquire)

Standard built-in air-fl ow rotameter (1-10Lpm for 5L vessels); adjustable draft tube; O2 enrichment optional

Gas mixing Manual / automatic air and O2 mixing; additional 4-gas mixing station available

Sparger Orifi ce ring sparger supplied as standard Micro-sparger

Inlet fi lter 0.2μM absolute fi lter

pH

Range 2-14 pH; 0.01pH resolution

Control PID, linked to acid/base pumps; one-point control with adjustable deadband (pH control range)

Sensor Gel-fi lled, autoclavable pH probe

DO

Range 0-200%; 0.1% resolution

ControlPID, 2-stage cascade: starting with agitation (1), cascading to optional O2 enrichment (2) if agitation speed is maximum but DO is below setpoint; substrate feeding programme (3) activated at DO setpoint

Sensor Autoclavable, polarographic DO probe

ANTIFOAMControl On/off

Sensor 316L stainless steel with insulated Tefl on tube

EXHAUSTFilter 0.2μM absolute fi lter

Condenser 316L stainless steel

PUMPS Control 4 built-in, assignable, with manual setting or programmable 15-step PID control; 0-65rpm (RE-485 connection on control station for fi fth pump option)

UTILITIES Water / gas / power 2 bar maximum (29 PSI) / 1 bar maximum (14.5 PSI); dry and oil-free / 100-120V 50/60Hz or 210-230V 50/60Hz; electrical safety cut-off switch

*Specifi cations may be subject to change without notice


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TECHNICAL SPECIFICATION*

Dry-Heating Bioreactor and Fermentation Systems (CSLFS-02)Heat-blanketed single-wall round bottom vessel systems Single-wall plain bottom vessel systems with heating base

VESSEL

Series CSLFS-V-B CSLFS-V-D

Working volume 3L 5L 7L 10L 15L 20L 3L 5L 7L 10L

Total volume 3.7L 6.8L 8.9L 12.5L 18L 24.4L 3.7L 6.8L 9.1L 13L

Design All vessels comprise autoclavable borosilicate glass contruction with 316L stainless steel headplate with fi ttings

CONTROL

STATION

Design features(A) Compact control station with integrated controller including built-in pumps and side panel capable of supporting utilities; (B) storage for 59,994 process programmes; (C) log data storage for 10 process monitoring fi les; (D) real-time trend data recording; (E) industrial standard ABS front panel with painted iron housing

Display 10.4-inch (265mm) colour touchscreen interface supplied as standard with each control station

Function Fermentation and cell culture monitoring and control

Dimensions Footprint (W x D): 400 x 500mm (15.75 x 19.69"); Height: 740mm (29.13")

TEMPERATURE

Range 5˚C above coolant to maximum 90˚C

Control(A) Dry-heating system with programmable 15-step PID control; (B) automatic solenoid-controlled cooling water valve

Dry-heating blanket and cooling loop (see A and B above) Dry-heating base unit and cooling loop (see A and B above)

Probe Platinum RTD probe (Pt100)

AGITATION

Drive Removable top 'brushless' motor

Range 30-1200rpm standard motor for routine fermentation and cell culture; 5-300rpm option for shear sensitive cell lines

Control Manual setting or programmable 15-step PID control

Impellers 3 pieces of adjustable Rushton-type impeller for less shear-sensitive microbial cell types (2 pieces of adjustable pitched-blade impeller for fragile cell types - optional)

Baffl es Removable 316L stainless steel baffl e for fermentation

AERATION

Gas fl ow optionsStandard built-in air-fl ow rotameter (1-10Lpm for 3/5L; 2-20Lpm for 7/10L; 4-50Lpm for 15/20L vessels); optional O2 enrichment module with rotameter and enrichment valve (bespoke TMFC possible - please enquire)

Gas mixing Manual / automatic air and O2 mixing; additional 4-gas mixing station available

Sparger Orifi ce ring sparger supplied as standard

Inlet fi lter 0.2μM absolute fi lter

pH

Range 2-14 pH; 0.01pH resolution

Control PID, linked to acid/base pumps; one-point control with adjustable deadband (pH control range)

Sensor Gel-fi lled, autoclavable pH probe

DO

Range 0-200%; 0.1% resolution

ControlPID, 2-stage cascade: starting with agitation (1), cascading to optional O2 enrichment (2) if agitation speed is maximum but DO is below setpoint; substrate feeding programme (3) activated at DO setpoint

Sensor Autoclavable, polarographic DO probe

ANTIFOAMControl On/off

Sensor 316L stainless steel with insulated Tefl on tube

EXHAUSTFilter 0.2μM absolute fi lter

Condenser 316L stainless steel

PUMPS Control 4 built-in, assignable, with manual setting or programmable 15-step PID control; 0-65rpm (RE-485 connection on control station for fi fth pump option)

UTILITIES Water / gas / power 2 bar maximum (29 PSI) / 1 bar maximum (14.5 PSI); dry and oil-free / 100-120V 50/60Hz or 210-230V 50/60Hz; electrical safety cut-off switch

*Specifi cations may be subject to change without notice


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DOUBLE-WALL ROUND BOTTOM SYSTEMSEach system includes: -

Controller Thermostatic system control station with built-in rotameter & 4x built-in peristaltic pumps

Vessel Double-wall vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors); 2 pieces adjustable pitched-blade impeller; & baffl e, condenser & sparger assemblies

Motor Agitation motor Probes 1x pH probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable

Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc fi lters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench

PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION

CSLFS-01-A03P-110/220VComplete thermostatic bioreactor and fermentation system with

double-wall round bottom vessel, 3LCSLFS-01-A07P-110/220V

Complete thermostatic bioreactor and fermentation system with

double-wall round bottom vessel, 7L Mammalian & insect cell culture

CSLFS-01-A05P-110/220VComplete thermostatic bioreactor and fermentation system with

double-wall round bottom vessel, 5LCSLFS-01-A10P-110/220V


double-wall round bottom vessel, 10L

SINGLE-WALL ROUND BOTTOM VESSEL SYSTEMSEach system includes: -


Vessel Single-wall vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors); 3 pieces adjustable Rushton-style impeller; & baffl e, condenser & sparger assemblies




CSLFS-01-B03P-110/220VComplete thermostatic bioreactor and fermentation system with

single-wall round bottom vessel, 3LCSLFS-01-A07P-110/220V

Complete thermostatic bioreactor and fermentation system

withsingle-wall round bottom vessel, 7L Anaerobic & aerobic microbial

fermentationCSLFS-01-B05P-110/220VComplete thermostatic bioreactor and fermentation system with

single-wall round bottom vessel, 5LCSLFS-01-A10P-110/220V


single-wall round bottom vessel, 10L

AIR-LIFTER VESSEL SYSTEMSEach system includes: -


Vessel Air-lifter vessel (including glass body, head plate, draft tube, T-shaped hand-lifters & 2 probe adaptors), condenser & micro sparger assemblies

Motor n/a Probes 1x pH probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable



CSLFS-01-C051P-110/220VComplete thermostatic bioreactor and fermentation system with

single-wall air-lifter vessel, 5LCSLFS-01-C052P-110/220V


double-wall air-lifter vessel, 5L

Culture of plant & shear-sensitive cells

HEAT-BLANKETED SINGLE-WALL ROUND BOTTOM VESSEL SYSTEMSEach system includes: -

Controller Dry-heating system control station with built-in rotameter & 4x built-in peristaltic pumps

Vessel Single-wall round bottom vessel with heating blanket (including glass body, head plate, draft tube, T-shaped hand-lifters & 2 probe adaptors); 3 pieces adjustable Rushton-style impeller; & baffl e, condenser & sparger assemblies




CSLFS-03-B03P-110/220VComplete dry-heating bioreactor and fermentation system with

single-wall round bottom vessel, 3LCSLFS-02-B10P-110/220V

Complete dry-heating bioreactor and fermentation system with

single-wall round bottom vessel, 10L Fermentation of anaerobic & aerobic microbes & culture

of temperature- and shear-sensitive cells









SINGLE-WALL PLAIN BOTTOM VESSEL SYSTEMS WITH HEATING BASEEach system includes: -

Controller Dry-heating system control station with built-in rotameter & 4x built-in peristaltic pumps

Vessel Single-wall plain bottom vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors) with heating base; 3 pieces adjustable Rushton-style impeller; & baffl e, condenser & sparger assemblies




CSLFS-01-D03P-110/220VComplete dry-heating bioreactor and fermentation system with

single-wall plain bottom vessel, 3LCSLFS-01-D07P-110/220V


single-wall plain bottom vessel, 7LAnaerobic, aerobic &

micro-aerobic microbial

fermentationCSLFS-01-D05P-110/220VComplete dry-heating bioreactor and fermentation system with

single-wall plain bottom vessel, 5LCSLFS-01-D10P-110/220V


single-wall plain bottom vessel, 10L

PART NUMBER

PART NUMBER

PART NUMBER

PART NUMBER

For technical advice & further information & pricing on optional ORP probes, impeller choices, 5-300rpm agitation motor, external peristaltic pump, O2 enrichment

module & TMFC, please contact your distributor or email: sales@cleaverscientifi c.com.

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