best practices forbiobanking –examples from the leipzig ......also hairat roomtemperature leipzig...
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Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Best Practices for Biobanking– Examples from the Leipzig Medical Biobank
Dr. Ronny Baber
Biobanking 2019 BioTech Pharma Summit , February 15th 2019, Porto
Institute for Laboratory Medicine, Cinical Chemistry and Molecular Diagnostics
University Hospital Leipzig
Leipzig Medical Biobank –Leipzig Research Centre for Civilisation Diseases
University Leipzig
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
The Leipzig Research Center for Civilization Diseases (LIFE)
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
• Meeting der Geschäftsführer der Mitglieder der Landesexzellensinitiative Sachsen
• 22.02.2013
• Matthias Nüchter
Chemistry
Biochemistry
MPI
UFZ
Leipzig Research Center for Civilization Diseases (LIFE)
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Population based Biobanking in LIFE
(A) LIFE HEALTH (B) LIFE DISEASES• ADULT Depression 40-60 (n=3.300)• Child Depression (n=760)• Heart (n=7.000)• Head and neck cancer (n=120)
• ADULT (n=10.000)• Child (n=6.000, incl. 1.000 newborns)
(Leipzig population based study)
Which genetic and environmental factors cause theindividual variance of modern diseases?
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
The largest LIFE Cohorts
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Assessment in LIFE Child – a Selection
Poulin et al., Eur J Epidemiol, 2017
basal metabolic rateeye examination
questionaires
infant examinationdental examination
spirometry
singing & speaking voice
anthropometry
sport tests
ultra sound
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Follow up of the Child Cohort
Poulin et al., Eur J Epidemiol, 2017
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Assessment in LIFE Adult – a Selectionassessment examplesanthropometry 3D-bodyscan, height, weight
heart function echocardiography, heart ultrasound
vascular system carotid artery sonography, blood pressure
glucose metabolism oGTT, fasting glucose, HbA1c
physical activity, fitness and sleep 7-day actinometry
macula and glaucoma degeneration optical coherence tomography
neuroimaging and neurocognition MRI, cognitive tests
allergy prick-test
interviews, questionnaires smoking habit, nutrition, lifestyle
Loeffler et al., BMC Public Health, 2015
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Follow up of the Adult cohort
Loeffler et al., BMC Public Health, 2015
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
LIFE Leipzig Heart studyCarotid ultrasound Angiography
Ankle-brachial Index
ECG/Stress test
Echocardiography
Roland-Ernst-Foundation LIFE
2006 2010 2016
CAD: coronary artery disease
MI: myocardial infarction
Beutner et al., 2011, PLoS one
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Heart Study Follow Up
• Focus on patients without heart failure with preserved pump function
• Patients with complete biomarker and echocardigraphic data sets
• Surveys: cardiological complaints, cardiovascular events …
• Physical examinations: anthropometry, investigations of blood vessels and cardiological function...
Male Femalen 1030 506
Mean ± SD 66.2 ± 10.0 68.1 ± 10.1
Minimum Age 37.4 31.8
Maximum Age 96.3 92.8
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
General Workflow of Biosamples in LIFE
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
The LIFE-Biobank
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Preanalytical WorkflowAutomatic Aliquoting
Cell Lab
Sample Elevator
Preanalytical Work Area
Straws
Cryo vials
TemporaryStorage
max. 120 min
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Preanalytical Workflow
Blood Withdrawal immediate Registration and Centrifugation
Cloning
Adults
Children
max. 120 min
mean: 97 ± 51 min; 85.4 % within time
mean: 113 ± 59 min; 63.0 % within time
Parentsmean: 105 ± 51 min; 68.6 % within time
Freezing
Adult FUmean: 76 ± 26 min; 92.8 % within time
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Biobank Workflow
Temporary Freezer
Transport (dry ice)
Sorting
Channel in
Storage (automatically)
Storage (manually)
20 min for 700 samples 8 min for 300 samples
Consistent cooling chain
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Biological samples from study participants
- 80 C° < - 150 °CSerum x xPlasma (ETDA, Citrate, Li-Hep) xUrine x xBreast milk xDried blood spot cards xDNA/RNA xPeripheral mononuclear blood cells xStabilized whole blood xSaliva xStool x
also hair at room temperature
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Laboratory information management system = CryoLab (in-house product)
Ambulance
(e.g. patient encoding)
Preanalytics
(e.g. sample storage time)
Sample
(e.g. material)
Biobank
(e.g. sample location)
CryoLab
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
From Population Based to Healthcare integrated Biobanking
Biobank
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Leipzig Medical Biobank
LIFE EpidemiologicalBiobank
• Adults
• Children
LIFE Medical Biobank
• Heart
• Head/Neck Cancer
• Depression
Medical Biobank
• Cancer
• …
Healthcare Integrated Biobanking –Leipzig Medical Biobank
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Healthcare Integrated Biobanking –Leipzig Medical Biobank
Phenotype
Tissue Blood
Biobank
Tissue Blood
Special diagnostics:• genome wide genotyping• genome wide expression analysis• metabolomic analysis from dried blood cards
Routine diagnostics
Routine diagnostics
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Healthcare Integrated Biobanking –Leipzig Medical Biobank
Informed Consent
Liquid Biomaterial Tissue
Sample Preparation
Biobanking
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Healthcare Integrated Biobanking –Leipzig Medical Biobank
1649
198
371
Liquid SamplesTissue Samples TumorTissue Samples Normal
196 Patients with liquid Biosamples
110 Patients with corresponding Tissue samples
57
3633
30
65
29
esophagus stomachcolon rectumovary pancreasother
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
500.000
Samples In Samples Out
690.000
83.300
38.800
> 60 Projects
Balance of biobanked Samples
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Projects from LIFE and the LMBUnderstanding of Diseases• Interaction between diabetes and
allergy• Assessment of stress levels in saliva
and hair• Asthma and infection• Bone metabolism and growth• Iodine supply and thyroid function• Insulin secretion in oral glucose
tolerance test• brain serotyping• Ambrosia and Allergy• ApoE and Dementia• maternal nutrition and allergy
childhood development• Nutrition and Cognition• Sex hormones and brain structure• Adipozytokines and Diabetes• Plasticizers and childhood
development• …
Diagnostics and Therapy:• Diagnostics of cardiovascular diseases• Diagnostics of acute myocardial
infarction• Diagnostics of dementia• Diagnostics of depression• celiac disease screening• Reference values for steroids• Breast cancer and genetics• Lipid biomarkers and cognitive
performance• Biomarkers for the diagnosis of non-
alcoholic fatty liver disease in childrenand adolescents
• Intervention in the case of obesity• Biomarkers in Tumor Cachexia• Cutting cultures for investigating the
effect of chemotherapeutic agents• Tumor xenograft models for
investigation of the effect ofchemotherapeutics
• …
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
How to get our Samples and Data?
http://life.uni-leipzig.de/en/service/contact.html
• Be part of the University Leipzig or a collaboratingpartner (or contact us for advice)
• Inform about cohorts and data in the LIFE data portal(ldp.life.uni-leipzig.de)
• Write a project proposal and inform all respectivescientists and principle investigators about theproposal
• Present the proposal to the „Use and Access Committee“ (meets once per month)
• Contact LIFE-Data Management / Biobank after approval of the proposal to schedule logistics
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Leipzig Medical Biobank as part of the German Biobanking Alliance (GBA)
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Leipzig Medical Biobank as part of the German Biobanking Alliance (GBA)
GBANetwork of 11 BMBF-funded biobank sites Two IT expert centresInclusion of further biobanksCoordinated by the German Biobank Node (GBN -Representation of German biobanks in Europe (BBMRI-ERIC))
Leipzig Medical Biobank
harmonization and standardization of biobanking processes in different parts of the biobanking fieldmake biomaterials from various biobanks available for biomedical research
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Work packages in GBA
WP5 Ethical, Legal
and SocialIssues (ELSI)
WP1 Central Executive
Management Office
WP2 The Biobanking IT
Network forGermany andBBMRI-ERIC
WP3 The Biobanking Quality System
WP4 Public Accountability,
Public Relations, Public Outreach
WP6 Counsellingbiobanks,
Education andTraining
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Stakeholder Management in GBA
Technicalstaff
User: academia
User: industry
Donors/ patients
Potential user
Public/experts
Biobanks
Potential user survey
Workshopdonor
empowerment
User satisfaction
survey
GBN products & services survey
Technicians’educational
needssurvey
Workshops
StakeholderDialogue Forum I
User workshop: Evaluation ofIT search pilot
Qualitativeinterviews
StakeholderDialogue Forum II:
Industrycooperation
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Summary
• The Leipzig Research Center for Civilisation Diseasescollected biosamples and data in a population based way.
• Within the last years the infrastructure of the LIFE-Biobankwas used to collect samples and data from cancer patients.The Leipzig Medical Biobank (LMB) was born.
• Samples and data from the Leipzig Medical Biobank wereused for different kinds of projects.
• The Leipzig Medical Biobank is part of the German BiobankAlliance reaching out to different Stakeholders in order toharmonize and standardize biobanking processes in differentparts of the biobanking field.
Thanks to:
LMB Team:Dr. Juliane DorowMadlen SiegemundAngelina MehrländerKristin KolbeBritt RosinRobin ScheunerSimone ScharfAlexander Kiel
Institute for Laboratory Medicine:Prof. Dr. Joachim ThieryProf. Dr. Uta Ceglarek
Cancer Center Leipzig:Prof. Dr. Florian LordickProf. Dr. Ulrich Hacker
Institute for Pathologie:Prof. Dr. Christian WittekindDr. Tina Unger
Surgery:Prof. Dr. Ines GockelDr. Orestis Lyros
LIFE Administration:Dr. Matthias Nüchter
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Leipzig Research Center for Civilization Diseases
This publication is supported by LIFE - Leipzig Research Center for Civilization Diseases, Universität Leipzig. LIFE is funded by means of the European Union, by the European Regional Development Fund (ERDF) and by means of the Free State of Saxony within the framework of the excellence initiative.
Thank you for your attention!www.life.uni-leipzig.de