beyond pglo
DESCRIPTION
Beyond pGLO. Genetic Engineering and Stem Cells. Restriction enzymes: Have sticky ends or overhangs of DNA. This helps to “glue” in the gene of interest in the right direction. Must find a plasmid with the appropriate sticky ends that match your gene. Sticky Ends. Polymerase Chain Reaction - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/1.jpg)
Genetic Engineering and Stem Cells
*Beyond pGLO
![Page 2: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/2.jpg)
*Sticky Ends
*Restriction enzymes:*Have sticky ends or
overhangs of DNA.*This helps to “glue” in the
gene of interest in the right direction.*Must find a plasmid with the
appropriate sticky ends that match your gene.
![Page 3: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/3.jpg)
*PCR
*Polymerase Chain Reaction*Taq polymerase*Nucleotides*Thermocycler
*http://www.dnalc.org/resources/animations/pcr.htm
![Page 4: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/4.jpg)
*iPS stem Cells
![Page 5: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/5.jpg)
*Model for gene therapy
![Page 6: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/6.jpg)
*How might cell-based delivery avoid problems
associated with direct delivery
![Page 7: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/7.jpg)
*Pre-implantation genetic diagnosis
![Page 8: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/8.jpg)
*How do you tell a cell has a certain
gene?
*Gel Electrophoresis:*http://www.dnalc.org/resources/animations/
gelelectrophoresis.html
*Gene Chip:*http://www.sumanasinc.com/webcontent/ani
mations/content/dnachips2.html*How could gene chip technology transform
medicine as we know it?
![Page 9: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/9.jpg)
![Page 10: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/10.jpg)
*What is cloning?
*Learn how a mouse is cloned.*http://learn.genetics.utah.edu/content/cloni
ng/clickandclone/
*Many copies of identical mice are cloned from mouse embryos. You can have essentially 1000 identical twins with the same genetics.*This has been used in science for many
years.
![Page 11: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/11.jpg)
![Page 12: Beyond pGLO](https://reader036.vdocuments.net/reader036/viewer/2022062501/5681613f550346895dd0aa0c/html5/thumbnails/12.jpg)
*Problems with cloning from adult cells
*Epigenetic patterns already in place (how is DNA tagged to turn genes on and off?)*Transplantation process is costly.*Cells are genetically “older”. Telomerase
has been deactivated.