BiEau® Actif Green Algae

16906 BiEau® Actif Green AlgaeMoisturization + Improves Skin Barrier + Nourishing

Product Code: 16906

INCI Name: Spirulina Platensis Juice

INCI Status: Proposed

Suggested Use Levels: 1.0 – 10.0%

Suggested Applications: Enhances Moisturization, Improves Barrier Function

Solubility: Water Soluble

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• Plants naturally utilize and bind water in their cells as a mechanism of survival • Cellular water is the living energy for plant survival

• This cellular water or plant essence offers the ability to harness a nutritional, isotonicsolution capable of nourishing the skin and hair in personal care applications

• From cultured algal and mushroom cells to reanimated agricultural waste, Active Concepts maximizes raw material sourcing and creation for minimal environmental impact with the development of our BiEau® Actif botanical waters line

• BiEau® Actif Green Algae is the sustainably sourced essence of Spirulina platensis, designed to elevate skin moisture levels and promote effective beauty with an environmental conscience.

16906 BiEau® Actif Green AlgaeBackground

• Skin is the largest organ of the body and is composed of approximately 60% water

• As a protective barrier, the skin is our best defense against external aggressors

• Skin’s permeability can be influenced by a variety of environmental attributes -pollution particles, cosmetic actives, temperature fluctuations, and water exchange

• A delicate balance exists between water introduced onto our skin, using cosmetics, cleansers etc, and the water present in our skin and cells.

• Traditionally water used in the creation of cosmetic ingredients, typically lacks minerals and natural components that our skin craves

• Isotonic solutions offer an environment most beneficial and least disruptive to skin

16906 BiEau® Actif Green AlgaeWater is Life

• Isotonic solution – Equilibrium

• ‘Iso’ in Latin means equal

• Isotonic solutions have the same solute concentration on the inside of the cell as the outside of the cell

• Balanced condition between cells and environment

• Isotonic materials maintain the size, shape, and integrity of skin cells

• Hypertonic solutions cause cells to shrivel (dry out) while hypotonic solutions cause cells to swell (burst)

16906 BiEau® Actif Green AlgaeSolution Impacts on Cells

• Algae absorbs water and nutrients in their cells, directly from the surrounding water. • Depending on the species, fresh algae are 70-90 percent water by weight.

• This internal cellular water contains an abundance of biologically active components and is often disregarded in the extraction of algal lipids for biofuel.

• Nutrient-rich water of Spirulina platensis offers a cellular water capable of providing moisturization• Niacinamide naturally present – essential to strengthen the skin barrier and

increase skin moisture content• Niacinamide is converted in the body to NADPH

• A cofactor for the synthesis of fatty acids and lipids, such as ceramides, that are crucial for epidermal barrier function

16906 BiEau® Actif Green AlgaeCellular Water – Living Energy for Plant Survival

• We isolated the nutrient rich water from Spirulina platensis to create BiEau® Actif Green Algae, a vital botanical essence capable elevating moisturization and enhancing skin barrier

• BiEau® Actif Green Algae utilizes a novel approach to eco-conscious cosmetic active development while capitalizing on the presence of niacinamide from blue-green algae to promote hydrated, healthier-looking skin.

• BiEau® Actif Green Algae can be used in a variety of cosmetic and personal care formulations aimed to hydrate, harmonize, and nourish

16906 BiEau® Actif Green AlgaeBenefits

Protocol

• Equipment: DermaLab Combo

• Principle of measurement: Conductance, single frequency

• Subjects: 10 (m/f)

• Test area: Volar forearms

• Concentration used: 2.0%

• Frequency of application: Twice daily

• After four weeks the 2.0% BiEau® Actif Green Algae in base lotion moisturized skin 36.37% better than the base lotion alone

Figure 1. Comparative Moisturization.

16906 BiEau® Actif Green AlgaeMoisturization Assay

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T = 0 T = 24Hours

T = 1Week

T = 2Weeks

T = 3Weeks

T = 4Weeks

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Comparative Moisturization

Base Lotion vs. Untreated

Experimental (2.0% BiEau™ Actif Green Algae + Base Lotion) vs. Untreated

Experimental (2.0% BiEau™ Actif Green Algae + Base Lotion) vs. Base Lotion

Protocol

• Equipment: DermaLab Combo

• Principle of measurement: Open chamber, vapor diffusion gradient

• Subjects: 10 (m/f)

• Test area: Volar forearms

• Concentration used: 2.0%

• Frequency of application: Twice daily

• BiEau®Actif Green Algae is capable of efficiently reducing TEWL, helping to improve barrier function and allow for moisture retention.

Figure 2. Comparative TEWL.

16906 BiEau® Actif Green AlgaeTransepidermal Water Loss Assay

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T = 0 T = 1 Week T = 2 Weeks T = 3 Weeks T = 4 Weeks

Pe

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Comparative TEWL

Base Lotion vs. Untreated

Experimental (2.0% BiEau™ Actif Green Algae in Base Lotion) vs. Untreated

Experimental (2.0% BiEau™ Actif Green Algae in Base Lotion) vs. Base Lotion

Protocol

• Equipment: DermaLab Combo

• Principle of measurement: Conductance, single frequency

• Subjects: 10 (m/f)

• Test area: Volar forearms

• Concentration used: 2.0%

• BiEau® Actif Green Algae is capable of sustaining increased skin moisturization after application has ceased when compared to base lotion and untreated skin sites

Figure 3. Percent Difference in Moisturization After Application has Ceased.

16906 BiEau® Actif Green AlgaeMoisture Regression Assay

-40.00%

-20.00%

0.00%

20.00%

40.00%

60.00%

80.00%

100.00%

120.00%

T = -24 Hours T = -1 Week T = -2 Weeks

Pe

rce

nt

(%)

Dif

fere

nce

Moisture Regression

Base Lotion vs. Untreated

Experimental (2.0% BiEau™ Actif Green Algae + Base Lotion) vs. Untreated

Experimental (2.0% BiEau™ Actif Green Algae + Base Lotion) vs. Base Lotion

16906 BiEau® Actif Green AlgaeMoisturization + Improves Skin Barrier + Nourishing

Product Code: 16906

INCI Name: Spirulina Platensis Juice

INCI Status: Proposed

Suggested Use Levels: 1.0 – 10.0%

Suggested Applications: Enhances Moisturization, Improves Barrier Function

Solubility: Water Soluble

In-vitro

veganco

mp

liant

In-vivo

ISO-1

61

28

NI &

NO

I

BiEau® Actif Green AlgaeActive Concepts, LLCLincolnton, NC. USAwww. activeconceptsllc.com Office: +1 704 276 7100info@activeconceptsllc.com