bio-rad science ambassador program your profession is your passion. pass it on....

Bio-Rad Science Ambassador Program

Your profession is your passion. Pass it on.bio-rad.com/scienceambassadors

Bio-Rad Science Ambassador Program2

Focuses on isolating your own DNA from cheek cells The DNA will be transferred into an amulet The amulet will be a permanent keepsake of your genetic material —

your personal essence!

Today’s Activity

Genes in a Bottle Activity


Timeline

Genes in a Bottle Activity

Introduction

Background on DNA Extraction

Extract Genomic DNA from Cheek Cells

Prepare DNA Necklaces


What is DNA?


what can you see?

If you pull out one of your hairs,


Hair cells are found in the root of the hair


Hair cells have information about your hair


What are the parts of an animal cell?

A Cell


Cells come in all shapes and sizes

All have DNA!


DNA is inside cells

Cells are liken bags

that hold DNABreak open the bags and

out comes the DNA!


Protocol Highlights

Step 1: Use a simple water mouth wash to collect cheek cells

Step 2: Add lysis buffer to cells to break open cell and nuclear membranes and release nuclear contents

Step 3: Precipitate DNA with cold alcohol in high salt

Step 4: Transfer your DNA into the amulet


Illustrated Guide of Kit Components

Plastic transfer pipet

Micro test tube

15ml conical tube

Amulet


Zip-Top Bag Components

Workstation Inventory

15 ml conical tube 1

Colored micro test tube with protease/salt/lysis buffer 1

Disposable plastic transfer pipet 1

Necklace components 1

Tattoo 1

Small paper cup 1


Step 1: Cheek Cell CollectionIn this step, you will collect your cheek cells in a 15 ml conical tube using a water mouth wash.

1. Take out the 15 ml conical tube which contains 3 ml of water and label with your initials.

2. Practice using the transfer pipet by transferring 1 ml of water from a large cup to your small cup. Repeat 3 times for a total of 3 ml of water.

3. Take the water from the small cup into your mouth — don’t swallow it! Swish the water around like mouthwash and gently chew on the inside of your cheeks while you swish the water around for 30–60 seconds.

4. Carefully expel the water back into the small paper cup. Pour the water-cheek cell mix back into your 15 ml tube.


Step 2: Cell Lysis and ProteolysisIn this step, you will lyse your cheek cells and degrade the protein with a protease.

1. Open the colored micro test tube containing a protease/salt/lysis buffer mixture. Pour the entire contents into the 15 ml conical tube containing your cheek cells.

2. Place the cap on the tube and gently invert it 5 times — don’t shake it! Observe your tube — do you notice any changes?

3. Incubate for 5 minutes by warming the tube in your hand. This allows time for the protease to break down the proteins.


Step 3: DNA PrecipitationIn this step, you will precipitate your DNA with the addition of alcohol.

1. Hold your tube at a 45°angle and slowly fill the tube with alcohol by gently pouring or pipetting alcohol down the inside wall of the tube. Fill the tube to the 14 ml mark.

2. Cap your tube and let stand undisturbed for 5 minutes at room temperature. What do you see? (You should begin to see bubbles and white strands appearing at the interface between the alcohol and water phases — this is your DNA!)

3. Very gently tilt the tube on its side and then turn it upright about 10 times until the water and alcohol phases are mixed.

4. Once mixed, the DNA should be fully visible as a “precipitate”.


Step 4: Necklace AssemblyIn this step, you will assemble your DNA necklace.

1. Place the amulet on your desk. Using a plastic pipet, carefully transfer as much of your DNA precipitate and only as little alcohol as you can into the amulet. The amulet requires approximately 0.5 ml to fill.

2. Screw the cap onto the amulet.

3. Slip the waxed cord through the cap. Your DNA necklace is now complete!


Congratulations!

Genes in a Bottle™ Kit

You have just created your very own DNA necklace!


How long does the DNA in the necklace last?

The DNA in the amulet can last for years. Add more alcohol into the vial if some evaporation occurs.

Bulletin 6378 Rev A 13-0498 0313

bio-rad science ambassador program your profession is your passion. pass it on....

Documents

cells cells

cell slide

hair slide

cheek cells step

conical tube amulet

hair cells

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watercheek cell