bio research training iit delhi€research & training @ iit delhi on ‐ going programmes in...
TRANSCRIPT
Bio‐Research & Training @ IIT Delhi
On‐going Programmes in Life Sciences at IIT Delhi and Vision for Future
Prof. Surendra Prasad, Director
Prof. Saroj Mishra, Department of Biochemical Engineering & Biotechnology
Prof. Harpal Singh, Centre For Biomedical Engineering
Prof. C. S. Dey, School of Biological Sciences
Prof. B. Jayaram, Chemistry, Supercomputing Facility & School of Biological Sciences
1
Bio‐Research & Training @ IIT Delhi
Evolution of Bio‐Engineering & Biosciences at IIT Delhi
1969: Centre for Biochemical Engineering (BERC)
1971: Centre for Biomedical Engineering (CBME) (Jointly by IIT & AIIMS)
1980: Biochemistry Cell
1989: Department of Biochemical Engineering & Biotechnology (DBEB)
2002: Supercomputing Facility for Bioinformatics & Computational Biology (SCFBio)
2008: School of Biological Sciences (SBS)
2
Bio‐Research & Training @ IIT DelhiTeaching and R & D Focus
Departmet of Biochemical Engineering & Biotechnology (DBEB)HRD Programmes: Dual Degree B.Tech & M. Tech. & Ph.D. (supported by DBT) Research Focus: Industrial Biotechnology, Primary & Secondary Metabolites, Process Optimization & Scale up, Bioseparation, Bioreactor design, Bioorganic Synthesis, Bioinformatics
Centre for Biomedical Engineering (CBME)HRD Programmes: Ph.D.Research Focus: Medical Devices & Diagnostics, Drug Delivery Systems, Nano‐medicine, TissueEngineering, Implantology
Biochemistry HRD Programmes: M.Sc. (as an integral part of M.Sc.Chemistry) & Ph.D.Research Focus: Applied Enzymology, Bioseparation, Biochemistry of Extremophiles
Supercomputing Facility for Bioinformatics & Computational Biology (SCFBio) HRD Programmes: Short‐term Training CoursesResearch Focus: Computational Genome Analysis, Protein Tertiary Structure Prediction & Computer Aided Drug Design
School of Biological Sciences (SBS) HRD Programmes: Ph.D.Research Focus: Infectious Diseases & Non‐communicable Disorders
3
Bio‐Research & Training @ IIT Delhi
Faculty Strength (50+)DBEB: 16+ ( Prof. Sunil Nath, Prof. G.P. Agarwal, Prof. V.S. Bisaria, Prof. Subhash Chand, Prof. Saroj Mishra, Prof. SN.Mukhopadhyay; Prof. T.R. Sreekrishnan, Prof. AK Srivastava, Prof. P.K. Roychoudhury, Dr. Vikram Sahai, Dr. Prashant Mishra, Dr. Atul Narang, Dr. D. Sundar, Dr. Ravikrishnan Elangovan, Dr. Ritu Kulshreshta, Dr. Shilpi Sharma)
CBME: 7 (Prof. Alok Ray, Prof. Harpal Singh, Prof. Sneh Anand, Prof. Nivedita Gohil, Prof. S.M.K. Rahman, Dr. Veena Koul, Dr. A. Mishra)
Chemistry: 10 (Prof. M.N. Gupta, Prof. B. Jayaram, Dr. Sunil Khare, Prof. P. S. Pandey, Prof. NalinPant, Dr. Shashank Deep, Dr. Pramit Chowdhury, Dr. V. Haridas, Dr. N. G. Ramesh, Prof. H.M. Chawla)
SBS: 8 (Prof. C.S. Dey, Prof. James Gomes, Dr. Aditya Mittal, Dr. Archana Chugh, Dr. BiswajitKundu, Dr. Manidipa Banerjee, Dr. Tapan Choudhury, Dr. Vivekanandan Perumal)
Other Departments: 15+ [Prof. S.N. Maheshwari (CSE), Prof. M. Balakrishnan (CSE), Prof. L.K. Das (IDDC), Prof. S. Pattanayek (CHE), Prof. S.D. Joshi (EE), Dr. S. Ghosh (HUSS), Prof N. Bhatnagar(Mech), Dr. P.M. Pandey (Mech), Prof. D.K. Pandya (Phy), Prof. B. Gupta (Tex), Dr. S. Ghosh(Tex), Dr. B. Satapathy (CPSE), Dr. S. N. Naik (RDAT), Dr. A. Mullick (RDAT), Dr. Satyawati Sharma (RDAT) ]
4
Research focus at DBEB
Bioreactor Design, Process Optimization and Scale‐up Enzyme Transformations (Lipase, β‐glucosidases)
Upstream: Microbial physiology, Protein chemistry and Protein engineering, Cloning and expression in E. coli and P. pastoris.
Bioprocesses: Optimization, Modeling, Design of bioreactors for microbial, plant and animal cell fermentations.
Downstream: Membrane based, Chromatography based, Aqueous two‐phase based Separation
Scale‐up: Bacterial, fungal, Plant cells
(Primary and secondary metabolites, Sugars as nutraceuticals and drugs,Recombinant HSA and interferon, Waste water treatment,biopesticides/biofertilizers)
5
Seeds of P. hexandrum
Germinated seedling
Callus culture
Suspension culture
Batch cultivation
Batch cultivation with fluorescence probe
Continuous cultivation with cell retention
Setric impeller
Plant Cell Culture Development and Large Scale Cultivation
Conessine, Azadirectin,Taxol, Artemisin
6
Recent Patents
Patents (U.S. and Indian)1. T7 RNA polymerase based novel Corynebacterium vectors(JKD, US patent)2. Process development for treatment of high strength wastes(TRS)3. Production of L‐lactic acid (PKRC)4. Use of soluble lignin extract for production of hydrolytic enzymes(VS,SM,VSB)5. A combination treatment for decolorization/detoxification of textile
wastes(TRS,MC,SM).6. Development of a suitable bioreactor system for azadirectin production by hairy
roots of Azadirecta indica (AKS,VSB,SS).7. Plane cell/hairy root cultivation using inert solid substrate (AKS,SS,SG).8. A process for enhanced production of bioactive compounds (VSB,AKS,AB,AJ,NG).
7
Technology Transfers and Consultancies
1. PenG acylase (Ranbaxy)
2. Process for animal feed (Tetragon Chemie, Bangalore)3. Biopesticides/Biofertilizers (Panacea Biotech) 4. Xylanase for pulp and paper (International Panacea Ltd.).5. An integrated process for production of urokinase by mammalian cell lines (in
process with BCIL)6. Recombinant Therapeutics (in process with Panacea Biotech)
8
Future Plans
Research Front:• Modification of Glycolytic pathway, Process optimization and scale up of
Pichia pastoris for therapeutics.
• Novel Enzymes (Laccases, Glycosyl Hydrolases)
• Plant‐microbe interactions, Regulation by RNA
• Molecular motors
• Anaerobic and aerobic processes for waste treatment
Infrastructure front:• Up‐gradation of Bioprocess Facility to state‐of‐the‐art centre
• Integrated Downstream Processing Facility for Proteins
• Protein Engineering Core Laboratory
• P2 Lab for Recombinant Therapeutic Protein Production
9
Medical diagnosticsPathogenic bacteria detection, Early cancer detection, Bioactive ABO bloodgrouping card, Metaland Iron measurement in biological fluids, Affordable and non‐invasive glucose monitoringsystems, Hormone detection in body fluids
Nanomedicine/Drug delivery systemsBiodegradable nanoparticle based drug delivery systems for diabetes and frostbite, nanoparticulatesystem for macrophage and cancer targeting, Polymeric antimicrobial agents for sterilization anddisinfection, Intranasal vaccine delivery
Tissue EngineeringPolymeric scaffold for cell growth and toxicity testing
ImplantologySoft and hard tissue implants, antimicrobial bone cement
Medical Devices and BioinstrumentationElectrically enhanced transdermal drug delivery systems,Low cost 12 lead ECG with telemetriccapability, Wireless ECG systems, intelligent above knee and below knee prosthesis
Research and Development Focus
Centre for Biomedical Engineering
10
S. No. Name of the instrument /product Name of Person Who Developed
Patent
1. Electrooculogram based Multi-mode Controller
Sneh Anand, Jayashree Santhosh 14606/DEL/2007
2. Device for External Counter Pulsation Therapy
Sneh Anand 2117/DEL/2008
3. Zig-G, A Wireless ECG system Sneh Anand, Abhinav, Meghna 683/DEL/2009
4. Bioactive ABO Blood grouping Card Harpal Singh 239/DEL/ 2005
5. A Pneumatic Damper Controlled AK Prosthesis
Sneh Anand, Manoj Soni, S. Maij
886/DEL/2009
6. Fluorescence based sensing of myoglobin and process thereof
Nivedita K. Gohil 2294 DEL 2007
7. A test kit and method for measurement of metals in biological fluids
Nivedita K. Gohil 2713 DEL 2008
8. Novel Clinical Kit to estimate iron overload in humans
Nivedita K. Gohil PCT/IN2009/000696
9. Composition of oral insulin delivery of therapeutic agent and processes thereof
Harpal Singh 1437/DEL/2006
10. A topical formulation for prevention and management of peripheral vascular diseases and a process of preparation thereof
Harpal Singh 1878/DEL/2009
Patents
11
S. No.
Technology /product Concerned Faculty Company
1. Electrically enhanced transdermal Drug Delivery System
Veena Koul,Sneh Anand
TROIKA Ahmedabad
2. Abdominal Stapler Dr. Anurag Srivastava,Sneh Anand
Johnsons & Johnsons, Mumbai
3. Antimicrobial acrylic bone Cement for fixation of Hip and Knee Joints
Harpal Singh XL- Orthomed,Faridabad
4. Heat sealable coatings for industrial and medical packagings
Harpal Singh Kumar Printers , Okhla, New Delhi ,
5. Immobilization of aminoacylase on functionalized acrylics for production of
6- aminopencillinic acid from pencillin.
Harpal Singh and S.Chand Ranbaxy Ltd. , Gurgaon, Haryana,
7. Bioactive ABO blood grouping card Harpal Singh Span Diagnostics, Ahmadabad (Under negotiation)
Know‐how transfer
12
Cellular fluorescence imaging (MCF‐7 cell line) with folated & non folated CdSe/CdS/ZnS‐MPA quantum dots
1 hr (folated) 4 hr (folated)
1 hr (non-folated) 4 hr (non-folated)13
0.000
20.000
40.000
60.000
80.000
100.000
120.000
3.38 6.75 12.50 25.00
%Vi
abili
ty
Conc. (pmol/well)
In-Vitro toxicity studies of Q.D.'s by XTT assay
CdTe CdSe/Cds/ZnS
40.000
50.000
60.000
70.000
80.000
90.000
100.000
10µg (25pmol of Q.D.'s ) 20µg (25pmol of Q.D.'s ) 40µg (25pmol of Q.D.'s ) 80µg (25pmol of Q.D.'s )
% viability
Conc. (µg/well)
In‐Vitro toxicity studies of Polymeric silica nanoparticles by XTT assay
In‐Vitro toxicity studies of quantum dots and quantum dots Doped polymeric silica nanoparticles for cancer
detection
14
Synthesis of super paramagnetic iron oxide nanoparticles and their evaluation for as MRI contrast agent and hyperthermia studies
SQUID analysis of Iron oxide NP's
‐5.00E‐03
‐4.00E‐03
‐3.00E‐03
‐2.00E‐03
‐1.00E‐03
0.00E+00
1.00E‐03
2.00E‐03
3.00E‐03
4.00E‐03
5.00E‐03
‐1.50E+04 ‐1.00E+04 ‐5.00E+03 0.00E+00 5.00E+03 1.00E+04 1.50E+04
Magnetic Field (Oe)
Mom
ent (e
mu)
15
CELLULAR INTERNALIZATION STUDIES OF FOLIATED IRON OXIDE NANOPARTICLES
1 hr 2 hr 4 hr 6 hr
1 hr 2 hr 4 hr 6 hr
SPIO‐FA
SPIO
16
Cellular Interaction studies of SPIO’s
Time dependent cell intraction studies of iron oxide NP's
-20.00
10.00
40.00
70.00
100.00
130.00
160.00
30 60 120 240 360 480 720
Time point
Iron
conc
/cel
l(pg)
SPIO-FA (NP's) SPIO (NP's) FA(TRTD) SPIO-FA
17
Antimicrobial Polymers (Sutures, Bone cement and water disinfection)
Modified Suture(3rd Day)
Commercially Available
Unmodified Suture
(3rd Day)
γ Rays / plasma exposure
O2
OOH
OOH
PP Matrix
Grafted PP Suture
Monomer(Acrylic Acid) N2
60° CNH3
Drug Drug
NH3+ +
Drug immobilized PP suture
18
BIOACTIVE BLOOD GROUPING CARD
Patent No. 239/DEL/2005Best Project Award in I2Tech 2005
Technology is ready for transfer and DST has approved the funding for multicentric clinical trials
19
In vivo evaluation studies of biodegradable nanoparticle based Parenteral depot of Insulin(single dose) in Diabetic
rabbits
20
Bio‐Research & Training @ IIT Delhi
Biochemistry1. Crosslinking for preparing Elisa conjugates & protein stabilization2. Bioseparation in the context of proteomics3. Efficient Biocatalyst design for low water media & MediumEngineering4. Enzyme based routes for Biodiesel propagation5. Efficient Biocatalyst Design for Biomass conversion inclusive ofmicrowave assisted pretreatment of cellulose, starch etc.6. Novel microbe based Genes & Enzymes for Food Bioprocesses(Detoxification & Value Addition)7. Chemical coatings in cosmetics and food sector for improvedlongevity
21
ChemGenome2.0
From Gene to Drug : The Dream @ SCFBio
Tertiary Structure
Sanjeevini
Genome
DNA
Protein
Drug
BhageerathSEEARTINSCIENCE……
Primary Sequence
RNA
22
SBS IITD 23
SCHOOL OF BIOLOGICAL SCIENCES
• Vision: To become the pioneers of modern interdisciplinary biologicalsciences by integrating emerging disciplines with biologicalsciences, and to nurture and sustain a vibrant comprehensiveprogramme in research and instruction.
• Mission:Promoting goal‐oriented innovative interdisciplinary researchby interfacing modern biology with applied engineeringsciences to address problems affecting human health andwelfare, and training scholars to be the next generationscientists
Focus: Infectious diseases & Noncommunicable disorders
23
Faculty and Expertise
Aditya MittalPhD: Drexel University; PDF: NIH
Kinetics and self assembly in biological systems.
Archana Chugh PhD: Delhi University; PDF: Lethbridge Research Centre
Cell penetrating peptides, Marine bioprospecting
Bishwajit Kundu PhD: IMTECH; PDF: Case Western
Protein misfolding and diseases, protein engineering
Chinmoy S. DeyPhD: Jadavpur University; PDF: CalTech & Baylor College of Medicine
Diabetes and infectious diseases
James Gomes PhD: Tulane; Industry: Process Service Inc, USA and Larsen & Toubro
Systems Biology
Contd.
24
Faculty and Expertise
B. Jayaram PhD: City Univ NY; PDF: Columbia University
Computational Biology
Manidipa Banerjee PhD, UCSD; PDF: Scripps Research Institute
Viral diseases, virus-based nanoparticles
Tapan K. Chaudhuri PhD; Bose Institute; PDF: University of Tokyo and Yale University
Protein folding and molecular chaperone
Vivekanandan PerumalPhD: CMC; PDF: Johns Hopkins
Infectious diseases and cancer biology
25
Research : Present and Future
• Current Research Focus– Mechanisms of protein‐protein interactions and
amyloidogenesis in neurodegenerative disorders– Production of therapeutic recombinant proteins through
chaperone assistance– Diabetes– Hepatitis B– Anti‐malarials– Cell penetrating peptides– Virus‐based nanoparticles– Bioprospecting
• Future research – Genomics and Proteomics of infectious and non‐communicable
diseases
26
Understanding disease mechanism, target identification and designing drugs
Viral diseases
Diabetes
AD, ALS
Structural BiologyAditya MittalBishwajit KunduB JayaramManidipa BanerjeeTapan Chaudhuri
Computational and Systems BiologyAditya Mittal B JayaramJames Gomes
Cell and Molecular BiologyArchana ChughBishwajit KunduChinmoy S DeyTapan ChaudhuriVivekanandan Perumal
Therapeutics
Archana ChughChinmoy S DeyManidipa BanerjeeTapan ChaudhuriVivekanandan Perumal
27
SBS IITD 28
Patents & Products– Anti‐diabetic drug screening (USPTO)
– Nanocarrier mediated plant transfection
– Anti‐leukemic protein drug
– Urokinase production
– Solid state reactor
Start‐up Companies– Initiated : 1
– Leadinvent : Computer Aided New Molecule Discovery
28
“Infected”sample
Total Protein, DNA
, RNA
Separation of Proteins and Nucleic Acids
High throughput Proteome and Genome
Genomic screening between control and
“infected”
Proteomic screening between control and
“infected”
Protein‐Protein Interactions, “Metabolomi
cs”
In vitro screening of infectious agent on cell lines (several with different
receptor biology)
Gene specific variations
Druggable target(s) identification
In silico drug design
Pre‐clinical (animal) and clinical testing*
Lead molecule synthesis*
*Will be outsourced, either to collaborativelaboratories or commercial organizations 29
Bio‐Research & Training @ IIT Delhi
Summary of Available Expertise at IIT Delhi
1. Devices & Diagnostics (Micro & Molecular)
2. Bioreactor Design, Process optimization & Scale up
3. Microbial & Enzyme Manipulations
4. Infectious Diseases & Non‐communicable Disorders (Experimental, Computational & Systems Biology Approaches) (BSL‐1, 2 & 3 level Labs in the making)
5. Bioenergy30
Bio‐Research & Training @ IIT Delhi
Proposed Challenges & Potential Areas of Partnershipfor the Consideration of DBT_IIT Consortium
1. Affordable Diagnostics/Devices
2. Design, Process Optimization & Scale up of Biological Processes (Microbial, plant & animal cell fermentation..)
3. Genomics/Proteomics & Diseases Target Identification & Database Creation (Neurodegenarative/diabetes etc.)
4. Novel Genes & Enzymes for Food Bioprocesses (Detoxification & Value Addition)
5. Bioenergy31
Bio‐Research & Training @ IIT Delhi
32