bio sefty and bio security
DESCRIPTION
ABOUT ANIMAL BUO SECURITY AMD BIOSEFTYABOUT HEALTH AND PRACTICAL THE VETERINARIANS TO HELP YOU IN FIELD AND EASY TO LEARN TO HELP THE VETERINARIANS, MEDICAL AND LABORATORIUM PRACTICETRANSCRIPT
BIOSAFETYBIOSAFETY
Keselamatan manusia dan Keselamatan manusia dan lingkungan yang berhubungan lingkungan yang berhubungan dengan seluruh pengaruhefek yg dengan seluruh pengaruhefek yg disebabkan oleh suatu penelitian disebabkan oleh suatu penelitian biologisbiologis
Beberapa hal yang perlu diperhatikan Beberapa hal yang perlu diperhatikan saat menggunakan hewan saat menggunakan hewan
laboratoriumlaboratorium
1048672 Allergens1048672 Zoonotic diseases1048672 Physical and chemical hazards1048672 Protocol related hazards
Personnel at riskPersonnel at risk
1048672 Animal Care Technicians1048672 Veterinarians1048672 Animal Researchers
Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab Risiko yg berhubungan dgn bahanagen yg akan
digunakan1048672 biological chemical physical toxical
Risiko yg berhubungan dgn spesies hewan lab yg akan digunakan1048672 zoonotic agents
Risiko yg berhubungan dgn pemeliharaan hewan1048672 ergonomic factors bites scratches allergens
Penilaian2 tsb dilakukan oleh kepala lab dekan kepala ACUC (Animal Care amp Use Committee)
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672Chemical agentscarcinogens mutagens
1048672toxic chemicalsanesthetics
1048672Physical agentsradiationheatsound
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672 Bahanagen biologis yg sangat membahayakanPenggunaan agen infeksius pada hewan lab
(infeksi buatan dgn agen infeksius)Pemeliharaan hewan yg diinfeksi selama
periode penelitianPada saat pemusnahan hewan nekropsi dan
pemanenan agenjaringan terinfeksi
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Keselamatan manusia dan Keselamatan manusia dan lingkungan yang berhubungan lingkungan yang berhubungan dengan seluruh pengaruhefek yg dengan seluruh pengaruhefek yg disebabkan oleh suatu penelitian disebabkan oleh suatu penelitian biologisbiologis
Beberapa hal yang perlu diperhatikan Beberapa hal yang perlu diperhatikan saat menggunakan hewan saat menggunakan hewan
laboratoriumlaboratorium
1048672 Allergens1048672 Zoonotic diseases1048672 Physical and chemical hazards1048672 Protocol related hazards
Personnel at riskPersonnel at risk
1048672 Animal Care Technicians1048672 Veterinarians1048672 Animal Researchers
Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab Risiko yg berhubungan dgn bahanagen yg akan
digunakan1048672 biological chemical physical toxical
Risiko yg berhubungan dgn spesies hewan lab yg akan digunakan1048672 zoonotic agents
Risiko yg berhubungan dgn pemeliharaan hewan1048672 ergonomic factors bites scratches allergens
Penilaian2 tsb dilakukan oleh kepala lab dekan kepala ACUC (Animal Care amp Use Committee)
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672Chemical agentscarcinogens mutagens
1048672toxic chemicalsanesthetics
1048672Physical agentsradiationheatsound
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672 Bahanagen biologis yg sangat membahayakanPenggunaan agen infeksius pada hewan lab
(infeksi buatan dgn agen infeksius)Pemeliharaan hewan yg diinfeksi selama
periode penelitianPada saat pemusnahan hewan nekropsi dan
pemanenan agenjaringan terinfeksi
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Beberapa hal yang perlu diperhatikan Beberapa hal yang perlu diperhatikan saat menggunakan hewan saat menggunakan hewan
laboratoriumlaboratorium
1048672 Allergens1048672 Zoonotic diseases1048672 Physical and chemical hazards1048672 Protocol related hazards
Personnel at riskPersonnel at risk
1048672 Animal Care Technicians1048672 Veterinarians1048672 Animal Researchers
Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab Risiko yg berhubungan dgn bahanagen yg akan
digunakan1048672 biological chemical physical toxical
Risiko yg berhubungan dgn spesies hewan lab yg akan digunakan1048672 zoonotic agents
Risiko yg berhubungan dgn pemeliharaan hewan1048672 ergonomic factors bites scratches allergens
Penilaian2 tsb dilakukan oleh kepala lab dekan kepala ACUC (Animal Care amp Use Committee)
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672Chemical agentscarcinogens mutagens
1048672toxic chemicalsanesthetics
1048672Physical agentsradiationheatsound
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672 Bahanagen biologis yg sangat membahayakanPenggunaan agen infeksius pada hewan lab
(infeksi buatan dgn agen infeksius)Pemeliharaan hewan yg diinfeksi selama
periode penelitianPada saat pemusnahan hewan nekropsi dan
pemanenan agenjaringan terinfeksi
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Personnel at riskPersonnel at risk
1048672 Animal Care Technicians1048672 Veterinarians1048672 Animal Researchers
Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab Risiko yg berhubungan dgn bahanagen yg akan
digunakan1048672 biological chemical physical toxical
Risiko yg berhubungan dgn spesies hewan lab yg akan digunakan1048672 zoonotic agents
Risiko yg berhubungan dgn pemeliharaan hewan1048672 ergonomic factors bites scratches allergens
Penilaian2 tsb dilakukan oleh kepala lab dekan kepala ACUC (Animal Care amp Use Committee)
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672Chemical agentscarcinogens mutagens
1048672toxic chemicalsanesthetics
1048672Physical agentsradiationheatsound
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672 Bahanagen biologis yg sangat membahayakanPenggunaan agen infeksius pada hewan lab
(infeksi buatan dgn agen infeksius)Pemeliharaan hewan yg diinfeksi selama
periode penelitianPada saat pemusnahan hewan nekropsi dan
pemanenan agenjaringan terinfeksi
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab Risiko yg berhubungan dgn bahanagen yg akan
digunakan1048672 biological chemical physical toxical
Risiko yg berhubungan dgn spesies hewan lab yg akan digunakan1048672 zoonotic agents
Risiko yg berhubungan dgn pemeliharaan hewan1048672 ergonomic factors bites scratches allergens
Penilaian2 tsb dilakukan oleh kepala lab dekan kepala ACUC (Animal Care amp Use Committee)
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672Chemical agentscarcinogens mutagens
1048672toxic chemicalsanesthetics
1048672Physical agentsradiationheatsound
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672 Bahanagen biologis yg sangat membahayakanPenggunaan agen infeksius pada hewan lab
(infeksi buatan dgn agen infeksius)Pemeliharaan hewan yg diinfeksi selama
periode penelitianPada saat pemusnahan hewan nekropsi dan
pemanenan agenjaringan terinfeksi
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672Chemical agentscarcinogens mutagens
1048672toxic chemicalsanesthetics
1048672Physical agentsradiationheatsound
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672 Bahanagen biologis yg sangat membahayakanPenggunaan agen infeksius pada hewan lab
(infeksi buatan dgn agen infeksius)Pemeliharaan hewan yg diinfeksi selama
periode penelitianPada saat pemusnahan hewan nekropsi dan
pemanenan agenjaringan terinfeksi
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Risiko yg berhubungan dgn bahanagen yg akan digunakan
1048672 Bahanagen biologis yg sangat membahayakanPenggunaan agen infeksius pada hewan lab
(infeksi buatan dgn agen infeksius)Pemeliharaan hewan yg diinfeksi selama
periode penelitianPada saat pemusnahan hewan nekropsi dan
pemanenan agenjaringan terinfeksi
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labAirborne (via udara)
ndash Terkontaminasinya udara dgn bahanagen infeksius akibat hewan bersin batuk
ndash Terkontaminasinya udara saat melakukan uji2 infeksi buatan via udara
ndash Terkontaminasinya udara oleh uap ekskreta urine amp feses hewan coba uap alas kandang
ndash Saat melakukan operasindash Saat membantu hewan partus
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labDirect Inoculation (Inokulasi langsung)
ndash Jarum suntik saat melakukan injeksiinokulasi hewan coba
ndash Gigitan amp cakaran hewan coba
Direct exposure of mucous membranes (by splash or splatter) (terpaparnya membran mukosa via percikan semburan)ndash Selama melakukan operasindash Saat menginfeksikaninjeksindash Saat melakukan nekropsi hewan coba
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Rute penularan agen biologis berbahaya selama bekerja dgn hewan labIndirect transmission and ingestion
ndash From contaminated hands or gloves to mouthndash Facial contamination directly from animalndash Transfer of parasites by animal handling
Indirect transmission with eye or mucousmembrane exposure
ndash Dust from beddingndash Splash during cage washingndash ldquoDirtyrdquo environment
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Bagaimana cara mengurangi risiko2 Bagaimana cara mengurangi risiko2 tersebuttersebutContainment of Infectious AgentContainment must include Primary containment
Enclosed filtered caging systemBiosafety cabinetsSafety equipmentPPE (Personal Protective Equipment)
Secondary containmentThe containment facility
bull Negative pressurizationbull Nonrecirculated air supplybull Ventilation must consider wellbeing of animal
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Beberapa contoh kandangsistem Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan perkandangan yg membatasi pergerakan bahanagen infeksius (bahanagen infeksius (Containment Caging Systems)
No Containmentndash Open (standard) cage
Some Containmentndash Filter top cage (microisolator cage)
Full Containmentndash Fully enclosed inventilated rack
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Beberapa Allergen Utama Menurut Spesies Hewan Lab Sumbernya amp Risiko yg ditimbulkannya
Species Allergen Source Relatif risk
RatsRats Rat n 1A Rat n 1B
UrineUrine ++++++++++
MiceMice Mus m1 UrineUrine ++++++++Guinea Guinea
pigpig-- Urine dander fur Urine dander fur
salivasaliva++++
gerbillsgerbills -- ++rabbitsrabbits glycoproteinglycoprotein Fur saliva urineFur saliva urine ++++++
catscats Fel d1 Sebaceus gland Sebaceus gland salivasaliva
++++++
dogsdogs Can f1 Saliva hair skinSaliva hair skin ++++sheepsheep -- LanolinLanolin ++pigspigs -- urineurine ++birdsbirds proteinprotein Faeces serumFaeces serum ++NHPNHP -- danderdander ++
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Risk of Developing Allergy to Laboratory Animals
Risk groupRisk group riskrisk historyhistory commentcommentnormalnormal No evidence of No evidence of
allergic allergic diseasesdiseases
~ ~ 1010 Minimal risk even upon Minimal risk even upon repeated exposurerepeated exposure
atopicatopic Pre-existing Pre-existing allergic diseaseallergic disease
Up to 73Up to 73 Risk increases with Risk increases with repeated exposurerepeated exposure
asymptomaasymptomatictic
IgE antibodies IgE antibodies to allergenic to allergenic proteinsproteins
Up to 100Up to 100 Risk of developing Risk of developing symptoms increases symptoms increases with repeated exposurewith repeated exposure
symptomatisymptomaticc
Clinical Clinical symptoms on symptoms on exposure to exposure to allergenic allergenic proteinsproteins
100100 33 with chest symptoms10 may developoccupational asthmaminimal exposure may leadto permanent impairment
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari KeterpaparanBeberapa prosedur utk menghindari
keterpaparan Gunakan pakaian khusus saat memasuki fasilitas
kandang hewan lab (bedakan pakaian kerja dgn pakaian sehari-hari
Gunakan personal protective equipment (PPE) yg benar
Selalu mencuci tangan dgn lar desinfektan setiap selesai bekerja dgn hewan lab
Kurangi transportasipemindahan hewan dari satu kandangbangunan ke kandangbangunan lain
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Minimizing Risk = Minimizing ExposureMenekan Risiko = Menghindari Keterpaparan
Beberapa saran dari aspek konstruksi fasilitas kandangndash Animal facilities have a relatively high air flow
which dilutes the concentration of allergens in the room air
ndash Animal facilities do not recirculate air unless the air is treated by HEPA filtration to remove allergens
ndash Animal facilities control relative humidity to 40-70 which reduces the bedding particle aerosolization
ndash Specialized caging is often used which minimizes potential allergen contamination of the environment
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Zoonotic Disease
Diseases transmitted between animals and humans under natural conditions Hal ini dipengaruhi oleh beberapa faktorndash Spesies hewanndash Sumber hewanndash Keganasan
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Transmission of Zoonoses
Enteric route (fecaloral)ndash Salmonella Shigella Campylobacterndash Giardia Toxoplasma Cryptosporidiumndash Entamoeba Hepatitis A
Respiratory routendash Q fever Chlamydia Measles
Skin contactndash Ringworm (Tinea) Measles Monkeypox
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Beberapa Spesies Hewan Lab amp Penyakit Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg DitimbulkannyaZoonosis yg Ditimbulkannya
Spesies hewanSpesies hewan PenyakitPenyakitMacacaMacaca B virus (Herpes B B virus (Herpes B
virus)virus)DombaDomba Q feverQ feverTikus putihTikus putih HantavirusHantavirusAnjing kucing skunks Anjing kucing skunks raccoons kelelawarraccoons kelelawar
RabiesRabies
Sapi non-human primatesSapi non-human primates TuberculosisTuberculosisKucingKucing ToxoplasmosisToxoplasmosisBurung beonuri (Parrots Burung beonuri (Parrots macaws)macaws)
PsittacosisPsittacosis
AyamunggasAyamunggas Avian InfluenzaAvian Influenza
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Rodents Rat Bite Fever
- Acute febrile disease following rodent bite- May exhibit rash on extremities- May be treated with penicillintetracycline
Leptospirosis- Contamination by urine or aerosols during cage
cleaning- May exhibit fever muscle pain headache- Could be fatal treatment with
penicillintetracycline
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Bagaimana mengontrol penyakit zoonosis Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)(Beberapa saranhellip) Get information on species and agent Quarantine animals prior to use Use Engineering controls
ndash facility construction and secondary barriers Consider the need for containment caging Use Administrative controls
ndash written SOPs and manuals Use PPE
ndash additional protection for worker Practice good facility and personal hygiene Provide staff training
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-
Safe Work Practices
Best defense against zoonotic disease is personal hygiene
Personnel working with animals must maintain a high standard of cleanliness
- BIOSAFETY
- Slide 2
- Beberapa hal yang perlu diperhatikan saat menggunakan hewan laboratorium
- Personnel at risk
- Beberapa penilaian risiko2 yg harus dipertimbangkan bila menggunakanbekerja dgn hewan lab
- Slide 6
- Slide 7
- Rute penularan agen biologis berbahaya selama bekerja dgn hewan lab
- Slide 9
- Slide 10
- Bagaimana cara mengurangi risiko2 tersebut
- Beberapa contoh kandangsistem perkandangan yg membatasi pergerakan bahanagen infeksius (Containment Caging Systems)
- Slide 13
- Risk of Developing Allergy to Laboratory Animals
- Minimizing Risk = Minimizing Exposure Menekan Risiko = Menghindari Keterpaparan
- Slide 16
- Zoonotic Disease
- Transmission of Zoonoses
- Beberapa Spesies Hewan Lab amp Penyakit Zoonosis yg Ditimbulkannya
- Slide 20
- Bagaimana mengontrol penyakit zoonosis (Beberapa saranhellip)
- Safe Work Practices
-