MCQs on Biochemistry

Harshraj Subhash ShindeK. K. W. CABT, Nashik

1.The term biochemistry was first introduced by

a)Karl Erekey b)Carl Neubergc)J Sumnerd)Svedberg

• Carl Neuberg

1.First protein sequenced by Frederick Sanger is

a. Haemoglobinb.Insulinc. Myoglobind.Bovine serum albumin

• Insulin

1.Nucleic acids in the nuclei of pus cell were firstly discovered by

a)Antone Lavoisierb)V. Mayerc)Helmholtzd)Friedrich Miescher

• Friedrich Miescher

1.The primary structure of protein represents

a. Linear sequence of amino acids joined by peptide bond

b.3-dimensional structure of proteinc. Helical structure of proteind.Sub unit structure of protein

• Linear sequence of amino acids joined by peptide bond

1.Tertiary structure of protein is maintained by

a. Peptide bondb.Hydrogen bondc. di-sulphide bondd.all of these

• all of these

1.Cobalt is part of … vitamin

a. Vitamin B1

b.Vitamin B2

c. Vitamin B3

d.Vitamin B12

a.Vitamin B12

1.Process of fermentation is firstly recognized by

a)Louis Pasteurb)Theoder Schwannc)V. Mayerd)Justus von Liebig

a)Theoder Schwann

1.Vitamins were discovered by …

a)Leonor Michaelisb)Liebigc)Casimer Funkd)V. Mayer

• Casimer Funk

1.–showed that viruses are nucleoprotein in nature

a)V. Mayerb)Justus von Liebigc)Wandell Stanley d)Leonor Michaelis

• Wandell Stanley

1.1gm of fat gives --- energy?

a. 3 kcalb.4 kcalc. 7 kcald.9 kcal

• 9 kcal

1.Fats are abundantly found in

a. Reproductive tissuesb.Vegetative tissuesc. Both a and bd.None of these

a. Reproductive tissues

1.Which of the following lipid is abundantly found in cell membrane?

a. Oleic acid b.Saturated fatty acidsc. Phospholipidsd.Linoleic acid

a. Phospholipids

1.Synthesis of glucose from fat is called

a. Glycolysisb.Krebs cyclec. Saponificationd.Gluconeogenesis

a. Gluconeogenesis

1.Hydrolysis of fats by alkalies into fatty acids and glycerol is called

a. Coagulation b.Saponification c. Suspension d.Colloidal

a. Saponification

1.β-oxidation takes place in

a. mitochondriab.cytoplasmc. chloroplastd.nucleus

Techniques in Biochemistry

• Paper chromatography is especially useful for the detection and separation of

1. Proteins2. DNA3. Lipids4. Amino acids

• Amino acids

1.is most commonly used filter paper for paper chromatography.

a)Whatman No. 1 paperb)Sterile paper c)Chromatography paperd)Cellulose paper

a)Whatman No. 1 paper

1.Whatman No. 1 filter paper is mainly composed of

a)Glutenb)Polysaccharidesc)Cellulosed)Starch

• Cellulose

• The method of electrophoresis is developed by

1. Tswett2. Sumner3. Tiselius4. Izmailov

• Tiselius

1.Combination of electrophoresis and chromatography is known as—

a)Gel electrophoresis b)Chromoelectrographyc) Electric chromatographyd)Ionophoresis technique

• Ionophoresis technique

1.Ultracentrifugation method was developed by

a)Tiseliusb)Svedbergc)Izmailovd)Sumner

• Svedberg

1.The 3-D structure of protein can be determined by

a. Nuclear magnetic resourceb.X- ray crystallographyc. Both a and bd.Spectrophotometry

• Both a and b

1.Disulphide bonds are formed between

a. Cysteine residue that are close togetherb.Methionine residue that are close togetherc. Proline residue that are close togetherd.Histidine residue that are close together

• Cysteine residue that are close together

• 28. Which of the polysaccharide is often called as ‘Animal starch’?

a. Pectinb.Dextrinsc. Glycogend.Xylan

C. Glycogen

•Which of the following country is major producer of agar-agar?

a. Koreab.Chinac. Thailandd.Japan

a. Japan

• Carbohydrates with free aldehyde / ketone groups known as –

a. Reducing sugarb.Non reducing sugarc. Polysaccharides d.All of these

• Reducing sugar

•Which of the following is reducing sugar?

a. Glycogenb.Inulinc. Sucrosed.Glucose

d. Glucose

•More than half the carbohydrate ingested by human is

a. Pectin b.Starch c. Glycogend.Cellulose

a. Starch

• Mixture of glucose and fructose is often called as

a. Invert sugarb.Table sugarc. Non reducing sugar d.Polysaccharide

• Invert sugar

Polysaccharide found in fungi and arthropods isa. Chitinb.Starchc. Xyland.Cellulose

• Chitin

1.Sucrose is disaccharide made up of

a)Glucose and fructoseb)Glucose and lactosec)Glucose and glucosed)Glucose and galactose

a)Glucose and fructose

1.Positively charged basic amino acids are

a. Lysine and arginineb.Lysine and asparagine c. Glutamine ans arginined.Lysine and glutamine

• Lysine and arginine

1.Aromatic amino acids includes

a. Phenylalanine, tyrosine and tryptophanb.Phenylalanine, serine and tryptophanc. Threonine, tyrosine and tryptophand.Aspargine, tyrosine and tryptophan

a.Phenylalanine, tyrosine and tryptophan

1.Sulphur containing amino acids are

a. Cysteine and methionineb.Methionine and threoninec. Cysteine and threonined.Cysteine and serine

• Cysteine and methionine

1.The simplest amino acid is

a. Prolineb.Methionine c. Glycined.Serine

• Glycine

Animal Biotechnology

1.The process of introduction of foreign DNA into an animal cells is called

a. Transversionb.Conversionc. Inversiond.Transfection

• Transfection

1.Maximum applications of animal cell culture today is in the production of

a. Insulinb.Interferonc. Vaccinesd.Edible protein

• Vaccines

1. Genes have been transferred into animals with a view to obtain a large scale production of the proteins encoded by these genes in the milk, blood etc. This approach is also referred generally as

a. Gene therapyb. In vitro culturec. Molecular farming d. Hybridoma technology

• Molecular farming

1.Which one of the following organism is used for the large scale production of recombinant insulin?

a.E. colib.Agrobacteriumc. Rhizobiumd.Plasmodium

• E. coli

Bioinformatics

1.The first bioinformatics database was created by

a. Pearsonb.Dayhoffc. Richard Durbind.Dayboff

• Dayhoff

1.Which of the following is an E.coli model organism database

a. Ecogeneb.EcoBasec. EcoSeqd.Colgene

• Ecogene

1.Single piece of information in a database is called

a. Fileb.Fieldc. Recordd.Data set

• Field

1.GenBank, the nucleic acid sequence database is maintained by

a. SWISS PORTb.EMBLc. DDBJd.NCBI

• NCBI

1.A database of current sequence map of the human genome is called

a. OMIMb.HGMDc. Golden pathd.GeneCards

• Golden path

1.An example of Homology and similarity tool?

a. PROSPECTb.EMBOSSc. RASMOLd.BLAST

• BLAST

1.Each record in a database is called

a. Entry b.Filec. Recordd.Input

• Entry

1.MatDB is a model organism database for

a. Humanb.Ricec. Arabidopsisd.Mouse

• Arabidopsis

1.Phylogenic relationship can be shown by

a. Dendrogramb.Gene Bankc. Entrezd.Data search tool

• Dendrogram

1.Clustal W is

a. Multiple sequence alignment toolb.Protein secondary structure predicting toolc. Data retrieving toold.Nucleic acid sequence analysis tool

• Multiple sequence alignment tool

1.The identification of drugs through genomic study

a. Genomicsb.Cheminformaticsc. Pharmacogenomicsd.Pharmacogenetics

• Pharmacogenomics