biocompatibility of differently cross-linked collagen...
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Osteoblast-like cells
Bio-Gide Tutodent Ossix BioMend
m
2
Cel
ls/m
m2
Cel
ls/m
m2
PDL fibroblasts
Bio-Gide Ossix Tutodent BioMend
Results
Conclusion
Study design
Biocompatibility of differently cross-linked collagen membranes Excerpt from Rothamel D, Schwarz F, Sculean A, Herten M, Scherbaum W, Becker J: Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Clin Oral Impl Res 2004; 15: 443–449.
Geistlich Bio-Gide® promotes the attachment and the proliferation of human PDL fibroblasts and human osteoblast-like cells best.
• Invitro evaluation of biocompatibility in cell cultures of (1) human PDL fibroblasts and (2) human osteoblast-like cells.
• Six specimens of each membrane were cultured with the PDL fibroblasts (n=24) or the osteoblast-like cells (n=24) for 7 days.
• Adherent cells were stained with hematoxylin and counted using a light microscope; the cell density per square millimetre was calculated.
The density of both periodontal ligament (PDL) fibroblasts and osteoblast-like cells was highest on Geistlich Bio-Gide® after 7 days of culturing.
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e© Geistlich Pharma AG Business Unit Biomaterials Bahnhofstrasse 40CH-6110 Wolhusen
T +41 41 492 56 30F +41 41 492 56 [email protected] www.geistlich-pharma.com
Osteoblast-like cells
Bio-Gide Tutodent Ossix BioMend
m
2
Cel
ls/m
m2
Cel
ls/m
m2
PDL fibroblasts
Bio-Gide Ossix Tutodent BioMend
70
60
50
40
30
20
10
0
100
80
60
40
20
0Tutodent®
Tutodent®
Ossix®
Ossix®
BioMend®
BioMend®
64
94
61
84
44
41
12
0
Geistlich Bio-Gide®
Geistlich Bio-Gide®
PDL fibroblasts
Osteoblast-like cells
Cel
ls/m
m2
Cel
ls/m
m2
Biocompatibility of various collagenmembranes in cultures of humanPDL fibroblasts and humanosteoblast-like cells RothamelD,SchwarzF,SculeanA,HertenM,ScherbaumW,BeckerJ.BiocompatibilityofvariouscollagenmembranesinculturesofhumanPDLfibroblastsandhumanosteoblast-likecells.Clin.OralImpl.Res.15,2004;443–449 Abstract
The aim of the present study was to evaluate the biocompatibility of differently cross-linked collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Four collagen membranes [BioGide® (BG), BioMend® (BM), Ossix® (OS) and TutoDent® (TD)] were tested. Cells plated on culture dis-hes (CD) served as positive controls. Six specimens of each membrane were in-cubated with (1) human PDL fibroblasts [2 x 104 cells] (n=24), and (2) human os-teoblast-like cells (SaOs-2) [2 x 104 cells] (n=24) under standardized conditions. After 7 days, adherent cells were stained with hematoxylin and counted using a reflected light microscope and the cell density per square millimeter was calcu-lated. Additionally, cell morphology was investigated using scanning electron mi-croscopy (SEM). Cell counts were presented as means and standard deviations (cells/mm2) and analyzed for statistical difference using the Wilcoxon test: (1) CD (434 ± 76)>BG (64 ± 19)=OS (61 ± 8)>TD (44 ± 4)>BM (12 ± 5); (2) CD (453 ± 92)>BG (94 ± 46)=TD (84 ± 49)>OS (41 ± 23)>BM (0). SEM examination revealed that PDL fibroblasts adherent on BG, TD and OS appeared spindle-shaped and flat, like cells on CD. SaOs-2 osteoblasts adherent on CD were star shaped and flat, but mostly round in shape on BG, OS and TD. BM appeared to be incompatible with the attachment and proliferation of SaOs-2 cells; however, a few PDL fibroblasts were found in a round shape. Within the limits of the present study, it was concluded that (i) BG, TD and OS promoted, and (ii) BM inhibited the attachment and proliferation of human PDL fibroblasts and human SaOs-2 osteoblasts.
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