RMC manufactured -DfE Biozyme - Biodegradable Industrial degreaser A compilation by Hussam Adeni

hussam_adeni@yahoo.com Lubricant Specialist & Tribologist

Trial and Performance report

RMC product

DfE Biozyme

Biodegradable

Industrial Heavy Duty Degreaser.

Hussam Adeni – Compilation (Collated during my tenure with Reza Industrial Solution.

Jeddah Saudi Arabia, 2014-15)

www.rezahygiene.com

JeddahTel: +966 12 657 2877Fax: +966 12 657 2832hygiene@rezagroup.com

U.A.E.Tel: +971 4 380 5715Fax: +971 4 380 5905hygieneuae@rezagroup.com

RiyadhTel: +966 11 479 1906Fax: +966 11 478 9853hygiene@rezagroup.com

QatarTel: +974 4 44160824Fax: + 974 4 44160513hygieneqatar@rezagroup.com

Al-KhobarTel: +966 13 802 4860Fax: +966 13 802 1302hygiene@rezagroup.com

BahrainTel: +973 17 699125Fax: +973 17 699061hygienebh@rezagroup.com

September 23, 2013 DfE Biozyme M Specifications

• Flammability: Non-flammable

• Maximum Storage Temperature: 120 degrees Fahrenheit

• Minimum Storage Temperature: 35 degrees Fahrenheit

• Optimal Storage Temperature: 40-100 degrees Fahrenheit

• Shelf Life: 2 years when stored as recommended

• Toxicity Material Tested Species LC50 (ppm) DfE Biozyme M Menidia beryllina 38.24 96-hr DfE Biozyme M Mysidopsis bahia 38.94 48-hr No. 2 Fuel Oil Menidia beryllina 3.76 96-hr No. 2 Fuel Oil Mysidopsis bahia 2.04 48-hr DfE Biozyme M & No 2 Fuel Oil (1:10) Menidia beryllina 6.88 96-hr DfE Biozyme M & No 2 Fuel Oil (1:10) Mysidopsis bahia 2.14 48-hr Reference Toxicant Menidia beryllina 12.19 96-hr (Sodium Laurel Sulfate) Mysidopsis bahia 10.53 48-hr Note: Toxicity data was derived using the concentrated product. Aquatic toxicity test method: US EPA 40 CFR Chapter 1, Part 300 Appendix C, Item 3.0: Revised Standard Dispersant Toxicity Test.

• Physical/Chemical Properties

Flash Point: Not Determined Pour Point: None Viscosity: Like Water Specific Gravity: Approximately 1 pH: 8.5-9.5

www.rochestermidland.com Corporate Headquarters 155 Paragon Drive Rochester, NY 14624 USA Tel: 585.336.2200 Fax: 585.467.4406

Surface Active Agents: Proprietary Solvents; Proprietary Additives: Proprietary Solubility: Complete in Water This formulation is a trade secret and comprised of a mixture of anionic and nonionic surfactants, preservatives, micronutrients and bacteria (Bacillus pumilus, Brevibacillus parabrevis, Paenibacillus validus) in water. The strains in this product have not been genetically modified or engineered. The species used in the product are recognized as Class 1 microorganisms (Risk Group 1; Biosafety Group 1) which means they pose little or no threat to humans.

• Biodegradability

This product contains Anionic Alcohol Ethoxylates and Dodecyl Benzene Sulfonic Acids; both classes of surfactants are regarded as readily biodegradable using the OECD Closed Bottle Test.

• Analysis for Heavy Metals, Cyanide, and Chlorinated Hydrocarbons

Compound Concentration (ppm)

Arsenic 0.0098 Cadmium <0.0020 Chromium 0.1190 Copper 0.0180 Lead <0.0040 Mercury <0.010 Nickel <0.0040 Zinc 0.0606 Cyanide <0.020 Chlorinated Hydrocarbons <1.0 Polychlorinated Biphenyls <0.100

 

 REZA Hygiene Technical Report

Field demonstrations of Biozyme M

Report Date 18/5/13 Report No. RH2 Author Andrew Turner Issued to Prasad Dias

Keith Watson

Investigation

We performed 3 separate tests on the Biozyme M product across 2 days. These test involved the following;

- Test 1 – Demonstration of product on fresh oil and crud removed from an offshore tank. This was poured over a steel pipe and left to dry for an hour before demonstrating the products ability.

- Test 2 – The product was tested on 2 onshore well heads which had recently had oil leaks

- Test 3 – The product was demonstrated on an offshore platform on 2 wellheads. These had sunbaked crude oil, dust and salt crust covering the equipment

All tests were carried out using the same batch of Biozyme M and dosed using a simple pump up sprayer. In a simple test of the products ability no proper agitation was used on any of the tests with the exception of high pressure hosing on the final offshore test site.

 

Results

Test 1 – Product Demonstration On this test fresh crude and silt from an offshore tank was poured over a stainless pipe and left to dry on for approximately 1 hour. Test details Product Concentration 10% (1:10) made up with sweet water Dosing method Portable pump up sprayer Contact time 1-2 minutes Agitation None used. Product just wiped off Rinsing None used The product was sprayed onto the area required to be cleaned and had immediate results as the crude started to come away from the steel tube straight away. After a dwell time of 1-2 minutes the tube was wiped cleaned with a rag leaving no trace of the crude oil at all. Fig. 1 Applying the product

 

Test 2 – Field Trial on onshore Wellheads Following the successful demonstration of the Biozyme M in a controlled environment we ventured out in the field to a wellhead that needed cleaning after suffering minor leaks. Test details Product Concentration 10% (1:10) made up with sweet water Dosing method Portable pump up sprayer Contact time 5-10 minutes Agitation None used. Product just wiped off Rinsing None used The wellhead was coated in oil that had started to dry out but retained some of its tackiness. The entire wellhead was sprayed with the Biozyme M solution and left to dwell for 5-10 minutes. We then wiped clean all the easily reachable areas with rack with a few repeat sprayings on thick areas of crude. The result was instant with the crude simply wiping away leaving the painted wellhead surface completely clean in most areas. Some areas would need more agitation and some sort of pressure rinsing but over all a good standard of clean was achieved in no more than 15 minutes. The top 60cm of the wellhead was not accessible. Fig 2. Before cleaning Fig 3. After cleaning Fig 2. Welhead prior to treatment

 

Test 3 – Field Trial on offshore wellheads Following the successful trials onshore it was decided that we should trial the Biozyme M product offshore on a wellhead platform. A suitable platform was chosen that had not been cleaned in a longtime. The platform in question had up to 2-3cm of baked on crude oil on the wellheads that was also covered in a thick layer of dust and salt. Test details Product Concentration 10% made up with salt water

50% & Neat also used Dosing method Portable pump up sprayer Contact time 30 minutes Agitation None used Rinsing High pressure jetting Initially we treated a wellhead with the same 10% solution used onshore left for 10-15 minutes minutes then rinsed off using high pressure jetting. The result was not as immediate as when used on the fresher crude but it did clean faster than normal jetting and leave a better result. As the trial progressed due to the difficulty in getting fresh water to mix the product we used it at higher concentrations and even applied neat in areas. It should be noted that Biozyme M will not increase in benefit as the concentration goes beyond 40-50%. The best range for use is 10-40% so a good supply of water is needed to make up solutions. We performed various test including cleaning half of a wellhead with just high pressure hosing and half treated with Biozyme M. The result was noticeably improved using Biozyme M on a number of counts;

- Time to remove the crude was much shorter - Overall appearance of the wellhead was better and removed more of the

crude to the painted surface level - Less work was needed with the high pressure hose. When just using the

hose it was held at 5-10cm from the wellhead to achieve the same result as 20-30cm away with Biozyme M.

 

Fig 4. Before Fig 5. After

 

Fig 6. Before and after when just using 15% Biozyme M

 

Summary

From our results we can conclude that the Biozyme M works excellently on fresh spillage cleanup on equipment and older (7-10 day) crude at levels as low as 10% (1:10). These results were achieved using minimal or zero agitation and no rinsing. On older baked on crude and higher concentrations of Biozyme M the result was not as instant but still gave a noticeable improvement in clean, decrease in cleaning time and decrease in cleaning effort. With the correct procedure Biozyme M could be used in many different areas to speed up the cleaning process. We would recommend a procedure along the lines of:-

1. Pre-Treat the effected area with 10-20% Biozyme M solution. The solution can be applied via pump up sprayer or buckets and brushes.

2. Leave solution to soak for 20-30 minutes 3. Scrub thicker areas of crude using brushes dipped in buckets of Biozyme

M solution. 4. Rinse using high pressure hosing 5. Repeat if necessary in areas of heavy soiling

Biozyme M has a residual effect so the product will continue to work for a few days afterwards. We would recommend using Biozyme M in place of any high hazard chemicals or traditional detergents used to remove crude oil as it has numerous advantages over other products, including;

- 100% Biodegradable. Waste biodegrades to just CO2 and H2O - pH 7.5 – 8.5 - Minimal (0) to slight (1) NFPA HMIS hazard codes - VOC compliant - EcoLogo certified - Degrades Polyaromatic hydrocarbons, Phenol, Cresol and BETEX

compounds - Can be used offshore with no need to retain rinsewater

 If the end goal is to remove organic solvents, caustics, acids and petrochemical based cleaners then a bacteria based biologic cleaning product is the only step to take.  

MATERIAL SAFETY DATA SHEET 204/13/2012

DATE PRINTED: 04/23/2012REVISION DATE:

PREPARED BY: EH&S DEPARTMENTREVISION NUMBER:

1. CHEMICAL PRODUCT PRODUCT NAME: DFE BIOZYME M, Bio-RemediatorPRODUCT CODE: 119276 NFPA/HMIS HAZARD CODES(minimal=0; slight=1; moderate=2; serious=3; severe=4)

2. HAZARDS IDENTIFICATION

EFFECTS FROM ACUTE EXPOSURE: INGESTION: Causes vomiting, nausea, and diarrhea.SKIN CONTACT: May cause mild skin irritation.INHALATION: May irritate mouth, nose, and throat.EYE CONTACT: May cause mild eye irritation.CHRONIC EFFECTS: Dermatitis. Possible respiratory damage from inhalation of dust or mist.EFFECTS/CARCINOGENICITY: None listed under OSHA, IARC, or NTP.ROUTES OF ENTRY: Routes of entry for solids and liquids include eye and skin contact, ingestion and inhalation. 3. COMPOSITION/INFORMATION ON INGREDIENTS

PRODUCT COMPOSITIONCAS#

% ACGIH TLV OSHA PELs

Viable Bacteria Cultures MIXTURE

<1 Not applicable Not applicable

4. FIRST AID MEASURES INGESTION: Rinse mouth with water many times. Drink several glasses of water or milk.SKIN: Wash with soap and water.INHALATION: Move person to fresh air.EYES: Immediately flush eyes with large amounts of water for at least 15 minutes. Get immediate

medical attention. NOTES TO PHYSICIAN: None. 5. FIRE AND EXPLOSION HAZARD DATA

METHOD: None FLAMMABLE LIMITS IN AIR

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Reactivity: 0/0 Special/Protective Equipment: None/BHealth:

FLASH POINT:

NAME OF THE MANUFACTURER:

(F): Not determined (C): Not determined

Rochester Midland Corporation155 Paragon DriveRochester, New York 14624 USA

1/1

Information: 585-336-2200 Emergency Phone: INFOTRAC: 1-800-535-5053 OUTSIDE US: 1-352-323-3500

Fire: 0/0

DFE BIOZYME M, Bio-Remediator____________________________________________________________________________________

LOWER (%): No data UPPER (%): No data SENSITIVITY TO MECHANICAL IMPACT(Y/N): NOSENSITIVITY TO STATIC DISCHARGE: Sensitivity to static discharge is not expected.SUITABLE EXTINGUISHING MEDIA: Water fog, carbon dioxide, foam, dry chemical.FIRE FIGHTING PROCEDURES: Fire-fighters should wear self-contained breathing apparatus and

full protective clothing when fighting chemical fires. 6. ACCIDENTAL RELEASE MEASURES SPILL PROCEDURES: SMALL SPILLS: Reclaim as much as possible. Flush residue with water. LARGE SPILLS: Reclaim as much as possible. Shovel or sweep up residue and place in suitable containers.

Flush remainder with water. PERSONAL PRECAUTIONS: Wear adequate personal protective equipment, see Section 8, Exposure Controls/Personal

Protection.ENVIRONMENTAL PRECAUTIONS: Not applicableMETHODS FOR CLEANING UP: Not applicable 7. HANDLING AND STORAGE PRECAUTIONS TO BE TAKEN IN HANDLING AND STORAGE:

Avoid generating or breathing dust. Store in a well ventilated area. Store in a cool, dry location away from incompatible materials. Keep container closed when not in use. Store between 32° F and 120°F. Avoid direct sunlight.

OTHER PRECAUTIONS: No other spill procedures necessary.SPECIFIC USE(S): Not applicable. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION PROTECTIVE EQUIPMENT:

EXPOSURE CONTROLS: Use in a well ventilated area.RESPIRATORY PROTECTION: Use approved NIOSH respiratory protection if TLV/PEL exceeded or if over exposure is likely.PROTECTIVE GLOVES: Chemical resistant gloves are recommended to minimize skin contact.EYE PROTECTION: Safety glasses are recommended to minimize eye contact.OTHER PERSONAL PROTECTION EQUIPMENT:

Appropriate protective clothing as needed to prevent skin contact. Eyewash fountains and safety showers must be easily accessible. It is the responsibility of the end user of this product to determine level of PPE required that is consistent with safe use of this product.

VENTILATION: General mechanical and/or local exhaust as needed to meet exposure limits if mist in air. 9. PHYSICAL AND CHEMICAL PROPERTIES APPEARANCE AND ODOR: Opaque. Liquid. Bacterial odor.

VAPOR PRESSURE: Not determined VAPOR DENSITY (AIR=1): Not determinedSOLUBILITY IN WATER: Not determinedSPECIFIC GRAVITY: Not determinedVOC Content (%): Not determinedEVAPORATION RATE: Not determinedPH: 8.5-9.5 10. STABILITY AND REACTIVITY STABILITY DATA: STABLE

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BOILING POINT (F): (C) Not determinedNot determined

DFE BIOZYME M, Bio-Remediator____________________________________________________________________________________

10. STABILITY AND REACTIVITYPOLYMERIZATION: Will Not Occur.HAZARDOUS DECOMPOSITION: None known.INCOMPATIBILITY (MATERIALS TO AVOID):

Contact with oxidizing agents.

CONDITIONS/HAZARDS TO AVOID: Strong acids or alkali compounds may inactivate biological cultures. 11. TOXICOLOGICAL INFORMATION ACUTE TOXICITY: Not EstablishedEFFECTS OF CHRONIC EXPOSURE: Not established.OTHER TOXIC EFFECTS: Not established. 12. ECOLOGICAL INFORMATION ECOTOXICOLOGICAL INFORMATION:

No data at this time

CHEMICAL FATE INFORMATION: No data at this time.MOBILITY: Not applicable.PERSISTENCE/DEGRADABILITY: Not applicableBIOACCUMULATIVE POTENTIAL: Not applicableOTHER ADVERSE EFFECTS: Not applicable 13. DISPOSAL CONSIDERATIONS WASTE DISPOSAL METHODS: Dispose in accordance with Federal, State and Local regulations. 14. TRANSPORT INFORMATION Please refer to the Bill of Lading/Receiving documents for up to date shipping information. 15. REGULATORY INFORMATION

PRODUCT COMPOSITIONCAS#

% TSCA: EINECS: Canada DSL: CA PROP 65:

Viable Bacteria Cultures MIXTURE

<1 Not Listed Not Listed Not Listed Not Listed

PRODUCT COMPOSITION

CAS# % CERCLA: SARA 302: SARA 313:

Viable Bacteria Cultures MIXTURE

<1 Not Listed Not Listed Not Listed

None of the components of this material are included in the Massachusetts Substance List nor are present at or above reportable levels.None of the components of this material are included in the New Jersey Substance List nor are present at or above reportable levels.None of the components of this material are included in the Pennsylvania Substance List nor are present at or above reportable levels. 16. OTHER INFORMATION This information was compiled from current, reliable sources and is believed to be correct. As data, and/or regulations change, and conditions of use and handling are beyond our control, no warranty, express or implied, is made as to completeness or continuing accuracy of this information.

*** END OF MSDS ***

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Networking – Memberships and Certificates Professional:

Hussam Adeni hussam57 Blog URL: http://lubricantspecialist.wordpress.com/

www.stle.org

www.lubecouncil.com

Society of Tribologists & Lubrication Engineer: Member – Individual

Membership number - 49912 M Certification: MLA 1

Section - International (99). Certified Level 1 Machinery Lubricant

Analyst, equivalent to Category I of

ISO 18436-4

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