bioe 410 term paper presentation

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  • 7/30/2019 BIOE 410 Term Paper Presentation

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    Johnson

    Huynh and

    Antonio Bunce

    INCREASING THE LIFETIME

    OF ARTIFICIAL LUNG

    DEVICE BY INCREASINGTHE BIOCOMPATIBILITY OF

    PDMS

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    ARTIFICIAL LUNG DEVICE

    [Joseph Potkay et al., Lab on a Chip, RSC Publishing, 2011]

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    DEVICE STRUCTURE

    [Joseph Potkay et al., Lab on a Chip, RSC Publishing, 2011]

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    DEVICE STRUCTURE

    Air

    PDMS Gas Diffusion

    Membrane

    Blood

    PDMS Channel

    PDMS Channel

    Front View Side View

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    Only hours

    Heparin and EDTA already used in blood

    Thrombus formation occurs in large channels and at size

    changes

    Non-coated tubing used to introduce blood to device

    DEVICE LIFETIME

    [Joseph Potkay et al., Lab on a Chip, RSC Publishing, 2011]

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    Use of Dermatan Sulfate in combination with

    Heparin to functionalize surface

    Use of fibronectin to endothelialize high

    aspect ratio channels

    Surface Modification through single ionirradiation

    METHODS OF INCREASING

    BIOCOMPATIBILITY

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    BIOACTIVE HEPARIN ON PDMS

    MICROFLUIDIC CHANNELS

    [Sara Thorslund et al.,Colloids and Surfaces B: Biointerfaces, Elsevier, 2005]

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    USE OF DERMATAN SULFATE TO

    INCREASE THROMBIN INHIBITION

    [Douglas Tollefsen, Progress in Molecular Biology and Translational Scienece, Elsevier, 2010]

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    Mucopolysaccharidoses

    Inhibit coagulation

    Mitral Valve Prolapse

    PROBLEMS WITH DERMATAN SULFATE

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    FABRICATION OF PDMS CHANNEL

    [Nisarga Naik, 33rd Annual International Conference of the IEEE EMBS,IEEE, 2011]

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    DIFFERENCE IN ARTIFICIAL LUNG DEVICE

    GEOMETRY

    Reference

    Paper

    Geometry

    Lung

    Device

    Made in SolidWorks

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    SURFACE MODIFICATION BY SINGLE ION

    IRRADIATION

    [M. Ionescu et al., Nuclear Instruments and Methods in Physics Research Section B:

    Beam Interactions with Materials and Atoms, 20th International Conference on IonBeam Analysis, 2012]

    PDMS Layer

    Ions

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    SIZE DIFFERENCES IN APPLYING TO GAS

    DIFFUSION MEMBRANE

    PDMS layer in ion irradiation

    paperThickness=4m

    PDMS gas diffusion layer for

    artificial lung deviceThickness =15m

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    Use of Dermatan Sulfate in combination with Heparin

    to functionalize surface

    Dermatan Sulfate has too many complications and is not well

    enough understood to be used

    Use of fibronectin to endothelialize high aspect ratio

    channels

    Can endothelialize all the sides of the blood channels

    Surface Modification through single ion irradiation

    Can increase cell adhesion to PDMS by up to 650%

    EVALUATION OF METHODS

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    OUR SOLUTION

    Blood

    PDMS Channel

    PDMS Gas Diffusion Membrane

    Endothelial

    Cells possibly

    due to ion

    irradiation

    Endothelial

    Cells due to

    fibronectin

    Fibronectin

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    REFERENCES

    D. M. Tollefsen, "Vascular Dermatan Sulfate and Heparin Cofactor II," Progress in Molecular Biology and Translational Science, vol.

    93, pp. 351-367, 2010.

    N. Naik, V. Kumar, E. L. Chaikof and M. G. Allen, "MEMS-assisted spatially homogeneous endothelialiization of a high length-to-depth

    aspect ratio microvascular network," 33rd Annual International Conference of the IEEE EMBS, pp. 290-293, 2011.

    M. Ionescu, B. Winton, D. Wexler, R. Siegele, A. Deslantes, E. Stelcer, A. Atanacio and D. Cohen, "Enhanced biocompatibility of PDMS

    (polydimethylsiloxane) polymer films by ion irradiation," Nuclear Instruments and Methods in Physics Research B, pp. 161-163,

    2011.

    W. C. Lamanna, R. Lawrence, S. Sarrazin and J. D. Esko, "Secondary Storage of Dermatan Sulfate in Sanfilippo Disease," The Journal

    of Biological Chemistry, 2011.

    "What is Dermatan Sulfate?," Conjecture Corporation, 2003-2012. [Online]. Available: http://www.wisegeek.com/what-is-dermatan-

    sulfate.htm. [Accessed 8 December 2012].S. Thorslund, J. Sanchez, R. Larsson, F. Nikolajeff and J. Bergquist, "Bioactive heparin immobilized onto microfluidic channels in

    poly(dimethylsiloxane) results in hydrophilic surface properties," Colloids and Surfaces, pp. 106-113, 2005.

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    Questions

    THANK YOU