Speaker- Siddhanta MohantyGuided by- Dr. B. Mitra

DEPARTMENT OF BIOTECHNOLOGY

Introduction Materials Methods Results Conclusion References

Ethanol is a desirable fuel because it allows fuel to burn more cleanly and lowers greenhouse emissions.

Bioethanol can be synthesized from cellulose and hemicellulose that originates from the many source of biomass.

Bioethanol burns to produce carbondioxide and water.

Cheaply available agricultural raw materials such as fruit pulp, rice extract, sweet potato, sugar cane, wood bark, maize husk and waste paper were used.

Enzyme such as amylase and cellulase were produced by Aspergillus niger and Trichoderma viride.

Amylase was assayed by starch plate method and cellulose was assayed by DNS mthod(Dinitrosalicylic acid).

10% of biomass was boiled in distilled water.

In case of fruit pulp, rice extract, sweet potato, potato and sugar cane are chopped, boiled and filtered.

ENZYME ASSAY

TWO STEP ENZYMATIC HYDROLYSIS

Extract was then sterilized, after sterilization 5% of enzyme was added for hydrolysis and incubated for 3 hrs at 37 degree Celsius.

In case of rice husk, wood bark, maize husk and waste paper (25gm) in 250ml distilled water are boiled as a whole and kept for sterilization.

After sterilization 5% of enzyme was added for hydrolysis at 37 degree Celsius for 3hrs of incubation and filtered aseptically.

Hydrolyzed and filtered extracts were fermented using Saccharomyces cerevisiae for 7 days of incubation at room temperature in rotary shaker.

FERMENTATION

Primary distillation was carried in rotary vacuum flask at 80 degree Celsius(boiling point of ethanol) and fraction is collected.

DISTILLATION

˃ Fermentation with sacharomyces.sp was done in anaerobic conditon.

S. No.

Sample Volume of extract before distillation in ml

Volume of extract after distillation in ml

Volume of Bioethanol in ml

1. POTATO 250 221 29

2. SWEET POTATO

250 216 34

3. FRUIT EXTRACT

250 211 39

4. RICE HUSK 250 233 17

5. WOOD BARK

250 241 09

6. SUGAR CANE

250 203 47

7. WASTE PAPER

250 232 18

8. MAIZE HUSK

250 231 19

9. LEAF LITTER 250 243 07

The bioconversion of waste to useable energy is also a part of utilization of waste.

Bioethanol can be synthesized from cellulose and hemicellulose that originate from many sources of biomass.

It become low cost than that of petrol. It create low pollution than other petroleum

products.

Bioethanol burns to produce carbondioxide and water on complete combustion as end products.

C2H5OH+3O2⇢2CO2+3H2O+HEAT Carbondioxide taken by plants , so it lowers

the pollution level.

Bioethanol was produced from various agricultural raw materials using two step enzymatic process, viz. enzyme hydrolysis followed by fermentation.

Journal of Advanced biotech, vol IX, issue 05, nov. 2009.

Chereminisoff,n.p,1979.gasohol for energy production. Ann Arbor Sci. Pub:12

Mohammad J. Taherzadeh, 1999. ethanol from Lignocellulose: Physiological effect of inhibitors. Chalmers University of Technology

Save plants Save energy

SAVE LIFE