biofilms research center for biointerfaces€¦ · happy that michael braian became swedish...
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BIOFILMSRESEARCH CENTER FOR BIOINTERFACES
AnnuAl RepoRt 2014
MALMÖ UNIVERSITYBIOFILMS – RESEARCH CENTER FOR BIOINTERFACES
Biofilms – Research Center for Biointerfaces
Annual Report 2014
Biofilms – Research Center for Biointerfaces (BRCB) is a translational research center
covering four research groups within
three faculties at Malmö University. The
core strengths of the Center are our broad
expertise, spanning the whole range from
theoretical modelling to clinical sciences,
and our long experience in working with
industry and relating to their needs. The
general aim of research activities at the
Center is to understand, predict and control
material/cell/tissue interactions with medical,
dental, food and environmental applications.
BRCB aims to become a regional site for
a creative biomedical technology environ
ment in the Öresund region, facilitating
collaboration between academic research,
higher education and industry, based on
pharmaceutical technology, biotechnology
and medical technology. Together, they
form a key area that offers significant
commercial growth potential. We are
striving to become a prime entry point
for regional industry seeking translational
academic expertise in the biomedical
technology field.
CENTER MISSION STATEMENT
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
DIRECTOR’S REpORT 4
NEwS 2014 5
wORkSHOpS AND CONFERENCES ORgANISED By THE CENTER
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RESEARCH HIgHLIgHTS 12
LIST OF CENTER MEMBERS 26
LIST OF RESEARCH ACTIvITIES
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SCIENTIFIC INSTRuMENTATION AND EquIpMENT
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COLLABORATIvE pARTNERS OF THE CENTER
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pHD THESES SupERvISED By CENTER MEMBERS
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LIST OF puBLICATIONS AT THE CENTER FROM 2014
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CONTRIBuTIONS TO AND pARTICIpATION IN CONFERENCES AND wORkSHOpS
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front illustration: Histological section of the bone to implant interface (Johansson p., Jimbo R., kjellin p., Currie F., Chrcanovic B., wennerberg A.Johansson, Biomechanical evaluation and surface characterization of a nano-modified surface on pEEk implants: a study in the rabbit tibia, Int J Nanomedicine. 2014 Aug 14;9:3903–11)
CONTENTS
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Annual Report 2014
DIRECTOR’S REpORT
2014 was a busy year for the Center and operations developed positively on many fronts. The administration staff at the Center and the Biomedical Sciences Research Group are both now in place in the new laboratory facilities adjacent to Medeon Science Park in Malmö and they are fully immersed in establishing a biomedical technology arena that will facilitate collaboration between academic research, higher education and industry. The goal is to create an innovation centre where researchers, students and entrepreneurs work side by side and share equipment and laboratories.
It has also been a year with the focus on PhD dissertations. Magnus Falk, Deyar Mahmood, Marjan Dorkhan, Ramesh Chowdhart, Anton Fagerström, Peter Lamberg, and Vida Krikstolaityte (Vilnius University), all successfully defended their PhD theses during the year. We are especially proud that Yana Znamenskaya’s thesis from 2013 “Effect of Hydration on Thermodynamic, Rheological and Structural Properties of Mucin” was awarded a prize at Malmö University’s Annual Ceremony in 2014 and that Marjan Dorkhan’s thesis “Effect of surface characteristics on cellular adherence and activity” was awarded the prize for the thesis of the year at the Faculty of Odontology. We are also happy that Michael Braian became Swedish champion in explaining his research and won the competition Forskar Grand Prix organized by the Swedish Research Council, Formas, Forte and VINNOVA.
When it comes to cooperation with industry, 2014 has been an exciting year. We successfully established new research collaborations with both local and inter national industrial
companies and enhanced research collaboration with Camurus AB and CR Competence AB by appointing Justas Barauskas and Tobias Haltur as adjunct researchers.
During 2014, we had a portfolio of 33 re search projects and collaborated with 33 different companies. We produced around 90 scientific publications in peerreviewed scientific journals and researchers at the Center actively contributed to the national and international research communities through involvement in organising international meetings, including the 15th International Conference on Electroanalysis, the 8th International Conference on Molecular Imprinting and the 10th Annual Workshop of Biofilms – Research Center for Biointerfaces. Other contributions to the research community have been made through membership of editorial and scientific advisory boards and as guest editors for highly reputed inter national journals, such as ‘Acta Biomaterialia’, ‘International Journal of Prosthodontics and Restorative Dentistry’, ‘American Journal of Dentistry, ‘ScienceJet’, ‘PeerJ’, Biosensors and Bioelectronics, and ‘Nonlinear Analysis: Modelling and Control’.
Finally, I would like to thank all those with whom we have worked for their invaluable contribution during the past year as well as Malmö University for its consistent support. I would also like to thank the Knowledge Foundation, the Swedish Research Council, the Borrow Foundation, the Swedish Laryng Foundation, the European Commission, the Skåne Region, the Crafoord Foundation, the British Medical Research Council and the Gustaf Th. Ohlsson Foundation who have funded our research.
Anna Holmberg, Director Biofilms
– Research Center for Biointerfaces
Interview with Michael Braian at http://www.tv4.se/nyhetsmorgon/klipp/han-vann-forskar-grand-prix-tack-vare-3d-t%C3%A4nder-3019489
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NEwS 2014
The new BRCB premises are bursting with activity now that researchers and students from the Department of Biomedical Science and the Center administration staff have moved in. The laboratories, which are adjacent to Medeon Science Park, are wellequipped with advanced instruments and firstrate analytical equipment. The researchers now have access to instruments such as AFM, DSC, Ellipsometers, LCMS, NMR, SEM, Sorption Calorimeter, In vitro Diffusion Equipment and QCMD.
“Our aim is to create an innovation centre where students, researchers, entrepreneurs
and companies work side by side, sharing equipment and laboratories,” said Anna Holmberg, Director.
“We hope to achieve our goal of becoming a regional meeting point and a creative environment for biomedical technology in the Öresund region, facilitating collaboration between academia, higher education and industry, based on pharmaceutical techno logy, biotechnology and medical technology. We are striving to become a prime entry point for regional industry seeking translational academic expertise in the biomedical technology field.”
new pRemises set to become An innovAtion centRe
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3. new ReseARch will incReAse knowledge of AtheRoscleRosisResearcher Marité Cárdenas Gómez at the Department of Biomedical Science has been granted SEK 8 million by the Swedish Research Council for a new research project, ‘Lipoprotein structure in the bulk and at the surface of vessel wall components’.
“We will study the structure of different lipoprotein particles. Lipoprotein is a biochemical assembly that contains both proteins
and lipids. The particles will be studied in solution and at the interface with the vessel wall,” she said.
By increasing knowledge of the structure and what takes place when lipoprotein is bound at the vessel wall, researchers hope to eventually come one step closer to developing diagnostics and treatment for atherosclerosis.
thesis of the YeAR 2014
Yana Znamenskaya was one of the prize winners at 2014 Annual Awards Ceremony, receiving the Thesis of the Year Award.
“I am very happy and proud to be honoured with the thesis of the year,” said Yana Znamenskaya. “The Department of Biomedical Science is an incredible research environment with high scientific standards and broad research field! I have had a great and professional supervision during my PhD study; without it nothing would be possible!”
THESIS ABOUT MUCUS
Yana Znamenskaya’s thesis “Effect of Hydration on Thermodynamic, Rheological and Structural Properties of Mucin” is focused on understanding of how the properties of the mucus change under different conditions, which is relevant for biomaterial coatings and drug delivery applications.
INCREASED RISk OF DISEASE
Mucus is a hydrated layer covering the epi thelium and is found in all internal tracts in the body – in the nose, mouth, stomach and intestines for example. One of its most essential functions is to protect the under lying tissue against dehydration, thus explaining why it contains large amounts of water. However, if the mucus comes into contact with dry air over a long period, it begins to dehydrate, reducing its protective properties and increasing the risk of disease.
MUCIN – A COMpLEx MOLECULE
In order to understand how mucus works,
Yana Znamenskaya made a study of its components. “The main component that is responsible for the protective properties of mucus is mucin, a complex molecule that we know very little about. This applies in particular to the very limited knowledge we have of how its properties are affected by hydration.”
“To investigate the fundamental properties of native tissues, especially how the highly hydrated layer covering mucosal surfaces of our body is affected by changes in ambient condition, I have studied how the mucin system is affected when the level of hydration and the temperature are changed.
SEVERAL AREAS OF USE
Yana Znamenskaya employed a whole series of methods and techniques in her research and she presents what are termed hydration curves, which describe how much water mucin can uptake at different hydration levels and temperatures. She also shows the conditions under which the transition from a glassy to an elastic state takes place.
“It is a complex system and we still have a great deal to learn although I hope that my research will be of use, for example, in enhancing transmucosal drug delivery and coatings of biomaterials.”
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ReseARch cAn contRibute to the eARlY discoveRY of dementiAProfessor Börje Sellergren has been granted funds by the Swedish Research Council for a research project dealing with diagnostic tools for neurodegenerative disease markers. The research involves developing tools for analysing lipidbased and proteinbased biomarkers in order to identify patients with cognitive neurological diseases before symptoms appear.
Apart from their wellknown structural function as a primary component in cell membranes, lipids have a whole range of biological functions and it has been shown that their existence in body fluids could reflect specific disorders, such as Alzheimer’s disease. However, at present there is an absence of practical methods for measuring these biomarkers clinically.
As part of an ongoing project, the research group has developed a highly sensitive method for the detection of very low lipid levels. In the new project, they will be able to use this method for the analysis of bioactive lipids in order to identify new biomarkers and to develop a specific, sensitive and rapid testing method for bloodbased lipid biomarkers.
“The aim is to develop new methods for the analysis of these biomarkers and for these methods to be brought into clinical use,” said Börje Sellergren. “The opportunity to reliably discover clinically accepted biomarkers for small vessel diseases and Alzheimer’s disease in the blood instead of cerebral fluid would simplify diagnostic procedures considerably. This would cut costs and involve fewer and less invasive interventions for the patient.”
Together with her research colleagues, Marité Cárdenas Gómez will produce biomimetic surfaces – surfaces that are designed using a special technique to represent biological surfaces. The researchers will attach different components from the vessel wall, which
is similar to cell membrane, to the surfaces. “We will then expose the biomimetic surfaces to lipoprotein to understand the nature of the interaction between lipoprotein particles and the biological surfaces.”
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Between seven and ten per cent of the population have periodontitis or caries, for which treatments are expensive and often applied at an advanced stage. Now Biofilms Research Center for Biointerfaces at Malmö University has received SEK 4.8 million from the KK Foundation to find new methods of attacking the problem at a much earlier stage.
“The film which forms on the teeth, both above and below the gumline, is really nothing other than a dense accumulation of bacteria”, says Gunnel Svensäter, Professor of Oral Health at the Faculty of Odontology at Malmö University.
“The bacteria above the gumline, on the part of the teeth exposed to the oral cavity, cause
caries. The bacteria below the gum cause periodontitis, that is inflammation and breakdown of the gums and bone, which slowly leads to loosening of the teeth”.
Currently, visits to the dentists mainly involve mechanically removing accumulated plaque and mending holes in the teeth. Treatment of caries and periodontitis is very expensive. Gunnel Svensäter hopes that the study, planned to run for four years and expected to generate at least six or seven research articles, will lead to completely new approaches.
“The study aims to find different tools to counteract the buildup of plaque before it takes off and to combat caries and periodontitis. It is about changing the conditions for
on the hunt foR oRgAnic methods AgAinst cARies And peRiodontitis
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How do you get a substance to penetrate the skin, a substance which can heal and do good? To get it through our barrier against the outside world, but not so far that it is transported away by the blood – enabling it to remain where its effects are needed? This is what Biofilms – Research Center for Biointerfaces at Malmö University is investigating in a new research project funded by the KK Foundation.
The outermost layer of the skin is only ten microns thick and yet it is the main barrier between the body and its surroundings. In total, skin is half a millimetre thick and its most important functions include protecting us against cold and dehydration. But sometimes, for example if we have eczema, we want the skin to absorb a pharmaceutical substance in a cream or ointment we apply to the exact spot we want to treat.
“What we are going to investigate is how to make it easier for the drug molecules to penetrate and then stay in the location where they can be useful”, explains Johan Engblom, who is a reader in Pharmaceutical Technology in charge of this research field at Biofilms – Research Center for Biointerfaces.
“The major challenge is to get the substance through the stratum corneum, the outermost layer of the skin which forms blisters when the skin is rubbed sore. It is often said that this layer accounts for approximately 80 per cent of the barrier properties. It consists of dead, flattened cells, whereas the underlying layer consists of living tissue. This is the level at which we want the substance to remain and be absorbed, rather than transported away from the site by the blood to affect the rest of the body”, says Johan Engblom.
This is precisely the advantage of allowing drugs to be absorbed through the skin: the parts of the body where the drug is not needed are not involved in the treatment through blood circulation or digestion, which limits the risk of sideeffects. The amount of active substance needed is also reduced when the drug is delivered through the skin instead of via the blood.
The project originates from research work conducted by Johan Engblom’s doctoral students and postdocs at Malmö University. The project has received SEK 4.8 million from the KK Foundation and partners are pharmaceutical companies Emeriti Pharma, Galenica
objective: to stAY just within the skin’s pRotective bARRieR
the bacteria. The bacteria which cause caries thrive in a low pH environment, i.e. an acid environment, and those which cause periodontitis do well in nutritious environments. In addition, the biofilms in which the caries and periodontitis are born have completely separate ecologies – they survive on different nutrients, have very different compositions, different physicalchemical conditions and different access to oxygen and nutrition”.
“What we want to find is an organic treatment; we will be investigating a number of substances which can change the environment for the bacteria and make it more alkaline and lean. These are the keys we are looking for”, says Gunnel Svensäter.
Naturally, it will take some time before the
market gets to see the results of the study in the form of a dietary supplement which can be taken to prevent caries and periodontitis. The study will be followed by a patenting process. The project is being carried out together with Lundbased companies Enzymatica AB and A1M Pharma. There are plans for both laboratory experiments and a clinical pilot study. “We have already worked together with Enzymatica and can see that both they and A1M Pharma are very well suited to our activities – they maintain a high academic standard and have a solid basis in our world”, says Gunnel Svensäter.
In addition to the project manager Gunnel Svensäter, the participants from Malmö University are Julia Davies, Claes Wickström, Mikael Sonesson and Jessica Neilands.
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AB and Bioglan AB. Gothenburg University is also in the group behind the application.
In fact the pharmaceutical substances involved are of secondary importance; the object of the investigation is how molecules with different chemical properties can more easily get to where they are needed.
“We have chosen a fatsoluble and a watersoluble model substance. These molecules can go different ways – it’s quite possible that a larger door will be opened for the watersoluble molecule than for the fatsoluble one, or vice versa. Most pharmaceutical molecules have oil properties, i.e. they are fatsoluble, whereas most creams are watersoluble.
Oil and water don’t mix. This is central to much of what we do here at the research centre. To simplify somewhat, creams are usually drops of oil in water, together with an emulsifier which makes the consistency smooth. But water drops in oil make the cream more like an ointment. Ointments are stickier and not as manageable as creams. There is more demand for creams. Gels consist largely of water which evaporates on application to the skin. Ointment, cream or gel – often a
company can choose to produce a variant of each with the same pharmaceutical substance, in order to cover a larger market segment. We are working with all three variants, but creams are the most common”.
Johan Engblom observes that the research network which is now being established in southern Sweden is strong and competitive, and will connect chemistry and medicine more closely. The project has been underway since March and will run for three years. From Malmö University, the participants are Johan Engblom, Tautgirdas Ruzgas, Peter Falkman, Cathrine Albèr and a new postdoc. Further participants are Henri Hansson and Anna Karin Morén from Galenica, Birgitta Svensson and Torbjörn Sund from Bioglan, David Gustafsson and Tomas Fex from Emeriti Pharma, and Marica Ericson from Gothenburg University. The project is expected to generate at least six research publications.
“We have a formalised timetable, but as with all scientific studies, new issues are bound to arise along the way and the lessons we learn from them will obviously affect the paths we choose in the project”, says Johan Engblom.
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wORkSHOpS AND CONFERENCES ORgANISED BY THE CENTER
The Center held the 10th Annual Work
shop in October. The focus this year was
Nanomedicine and the event attracted
more than 90 participants from different
universities as well as industry. Read more
about the workshop in the article below.
The 15th International Conference on
Electroanalysis was held at Malmö
University on June 11–15, 2014. These
biannual conferences have become
recognised worldwide as distinguished
electroanalytical meetings within the
framework of the European Society for
Electroanalytical Chemistry. The confer
ence covered virtually every aspect of
modern as well as classical electroanalysis,
ranging from theoretical approaches to
practical applications in different fields.
The conference attracted around 230
participants from all over the world and
was organised by Malmö University in
collaboration with Uppsala University,
the Technical University of Denmark
and Lund University. Tautgirdas Ruz
gas, Thomas Arnebrant and Sergey
Shleev were members of the organizing
committee.
http://eseac2014.com/
Börje Sellergren, who is coordinating
the EU FP7 Marie Curie Initial Training
Network PEPMIP – Robust affinity mate
rials for applications in proteomics and
diagnostics, organised the Lipidomics,
Proteomics and related technologies work
shop at Malmö University in November.
Also worth mentioning is the interview with Sergey Shleev in the TV programme TV4 Nyheterna:http://www.tv4.se/nyheterna/klipp/ny-k%C3%A4lla-f%C3%B6r-elektrisk-energi-presenteras-p%C3%A5-konferens-i-malm%C3%B6-2658757
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Each year, Biofilms – Research Centre for Biointerfaces holds a workshop aimed at researchers, industry and other stakeholders in life science and biomedicine. This year’s theme was nanomedicine – the use of nanotechnology in medical applications.
nAnomedicine in focus At AnnuAl woRkshop
Ulrik B Nielsen is CSO at Merrimack
Pharmaceuticals in Boston and one of the
founders of the company: “It has been
a very interesting workshop and with
excellent speakers. This is the first time
I’ve attended and I realise exactly how
valuable this workshop is for those of us
who work in the industry.”
Applications in diagnostics and treatment
The workshop was held for the first time at
Medeon, which is located beside the new
premises of Biofilms – Research Centre for
Biointerfaces and offered a whole range
of lectures and presentations in the field
of nanomedicine.
“Nanomedicine is about how nanotech
nology can be used for different medical
applications in order to diagnose or treat
patients. It is naturally extremely pleasing
to see such interest in the workshop and
that we have so many thought provoking
speakers,” said Anna Holmberg, Director
of Biofilms – Research Centre for Bio
interfaces.
pROMINENT SpEAkERS
During the workshop, prominent speakers
and representatives from industry spoke
on a variety of subjects, including how
different types of nanostructures and
nanoparticles can be used for implants
and drug delivery – across the skin, for
example.
“It is an extremely exciting programme
and a number of the presentations will
be of direct benefit to me in my work
on drug delivery,” said Louise Bastholm
Jensen from Leo Pharma.
MEASURINg SIgNALS IN THE BRAIN
Ulrik B Nielsen, Merrimack Pharma
ceuticals, spoke about his work with dif
ferent cancer therapies. The company has
several new treatments that will shortly
be brought to market. Professor Jens
Schouenborg from Lund University spoke
about the design of implantable electronic
interfaces for the brain. The aim is to be
able to connect the brain to a computer.
NANOSTRUCTURES
OFFER BETTER IMpLANTS
Representatives from Malmö University
included Professor Ann Wennberg, who
spoke about how nanostructures can
improve osseointegration. Professor
Tautgirdas Ruzgas spoke about a new
method for studying the absorption of
substances, such as drugs, across the skin.
“The presentations were incredibly
inspiring and are strongly linked to our
studies. I have acquired many new
ideas,” said Sviram Thoppe Rajendra, a
Master’s student at Biomedical Methods
and Technology, who was supported in
his view by fellow programme member
Linn Stenfelt.
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Different physicochemical properties (e.g. adsorption kinetics, thickness, viscoelasticity, mechanical stability, etc.) of adsorbed salivary pellicles depend on different factors including the properties (charge, roughness, wettability, surface chemistry, etc.) of the substratum. Whether these differences in physicochemical properties are a result of differences in the composition or in the organization of the pellicles is unknown. We have approached this by investigating pellicles eluted from substrata of different but wellcharacterized wettabilities by means of SDSPAGE. The resulting gels have been analyzed by various staining technique as well as immunoblotting procedures [1].
Whereas some compositional differences were observed, the stained SDSPAGE gels reveal a similar compositional pattern for the pellicles harvested from substrates of different wettability. In CBB stained gels (Fig. 1a), the more intense bands, i.e. those corresponding to MWs of ≈ 190 kDa, 56-59 kDa and 18 kDa, were found for all the investigated pellicles. Blotting experiments (Fig. 1b) suggested that the double band observed between 56-59 kDa could be attributed to amylases, specifically to the nonglycosylated and glycosylated isoforms. Blotting experiments also attributed the band observed at ≈ 18 kDa to cystatins. PAS stained SDSPAGE gels (Fig. 1c) of all the eluted samples demonstrated positive
glycoprotein bands at high MW (> 400 kDa, close to the wells of gels) and at a MW of ≈ 180 kDa. It is reasonable to attribute the high MW band to MUC5B and the 180 kDa band to MUC7.
Thus, our results showed that substratum hydrophobicity did not have a major impact on pellicle composition. In all substrata, the major pellicle components were found to be cystatins, amylases and large glycoproteins, presumably mucins. It is enlightening to discuss our results in terms of the wellaccepted twolayer model for salivary pellicles. This model states that, on most types of substrate, pellicles consist of an inner thin dense layer (which would be formed mainly by proteins of relatively low MW) and an outer thick diffuse layer (mainly composed of mucins). Our results showing the presence of mucins in all pellicles support their presence in the outer layer. Cystatins and amylases (in this order), will diffuse to the substrate faster than mucins because of having a lower MW being, therefore, the major components of the inner dense layer. In this scheme, substrate wettability will mainly affect the nature and magnitude of the forces driving the adsorption of the components of the inner layer and, subsequently, their orientation. In [1] it is discussed how this simple scheme would account for most of the physicochemical properties reported for salivary pellicles.
RESEARCH HIgHLIgHTS
influence of substRAtum hYdRophobicitY on sAlivARY pellicles: oRgAnizAtion oR composition?
[1] Aroonsang W., Sotres J., ElSchich Z., Arnebrant T., Lindh L. 2014. Influence of substratum hydrophobicity on salivary pellicles: organization or composition? Biofouling, 30: 1123-1132.
contact: [email protected]
1.
figure 1. a) Representative CBB-250-stained SDS-pAgE gels of pellicles harvested from substrates characterized by different water contact angles, . b) Representative illustration of immunoblots of the same pellicles as in a) probed with antibodies against amylase and cystatin-S. c) Representative pAS-stained SDS-pAgE gels of the same pellicles as in a). figure 2. Simplified scheme proposed for salivary pellicles.
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selection of multispecies heAlth-AssociAted biofilms using sAlivA As A nutRient souRceOral microbial biofilms support host defences by preventing colonization by exogenous pathogenic microbes. The integrity of mucosal surfaces relies, in part, on the ability of the hostderived mucus layer to aid colonization of commensal bacterial biofilms. In oral microbial biofilms (supragingival dental plaque) salivary proteins serve as a major ecological determinant of the composition of the microbial community, enables adhesion and growth, and are the major continuously available source of nutrients. To yield nutri
ents, the degradation of complex substrates, including salivary mucins, requires an arsenal of glycosidases and proteases. Several bacterial species, which possess complementary patterns of glycosidases and proteases, catabolize glycoproteins through a concerted action. In order to study how saliva and the oral bacterial community interact and how this interaction influences the composition and phenotype of the oral biofilm, supragingival plaque was grown in bacteria free human whole saliva as described below.
contact: [email protected]
Supragingival plaque samples were taken from healthy subjects and grown in 50% saliva for up to 7 days in 5% CO2. The microbial composition and enzymatic profile was determined after 0-, 2-, 5- and 7 days of growth. Similar changes in the microbial biofilms and enzymatic profiles were seen in three experiments. In all experiments, the enzymatic activity of galactosidase, galactosaminidase and glucosaminidase was increased and the enzymatic activity of glucosidase decreased
over 7 days. The sialidase and fucosidase activity remained the same. At the same time the protease activity increased. Hence, the enzyme profile of the biofilm changes due to the altered nutritional environment. This also indicates that the bacteria degrades and uses salivary mucins and proteins as nutrients. The saliva environment also seemed to select the growth of Actinomyces species and Capnocytophaga species.
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Mucins represent the major macromolecule comprising the mucus gels or glycalyx on the epithelial cell membranes. Biological functions of mucus gels are known to range from the protection of underlying tissues against external insult, mechanical stress, and pathogens to cell signal transduction. Inspired from their protective biological functions, mucins started to attract interest as coating materials for implants and biomedical devices.
We have investigated the mechanical stability of adsorbed mucin films (specifically bovine submaxillary mucin films) by means of an atomic force microscope operated in the friction force spectroscopy mode [1]. This methodology provides the strength of the adsorbed films in terms of the forces needed for breaking and removing them. More specifically, we have investigated how this stability depend on the pH of the ambient medium and on the wettability of the substrate. Our results showed that, for all studied pHs, mucin films formed on hydrophobic substrates are significantly stronger than those formed on hydrophilic substrates. Moreover, mucin films, formed on both hydrophobic and hydrophilic substrates, probed at acidic conditions are significantly stronger than those probed at neutral pH conditions.
These results have important implications not only for the understanding of the mechanical behavior of mucus gels and artificial mucin coatings, but for the understanding of salivary lubrication mechanism as well. Saliva has attracted an increasing interest as a waterbased biological lubricant due to its unique property of lubricating almost any type of surface. Salivary lubrication is attributed to the film of nanometric dimensions that forms immediately upon contact of saliva with any type of surface, i.e. the acquired pellicle. Mucins are one of the pellicle components shown to have a major impact of the friction reduction properties of the pellicle [2]. But a good lubricant not only reduces friction but must resist wear as well. A comparison (Fig. 1) between our results on the mechanical stability of mucins with those previously obtained on salivary films [2] reveals that, on hydrophilic substrates, saliva forms significantly stronger films than mucins do. This relationship also holds for films formed on hydrophobic substrates at neutral pH, while mucin films are stronger than salivary ones when formed on hydrophobic substrates at acidic conditions. Therefore, it is obvious that salivary lubrication, i.e. its ability to reduce friction and wear, results from the synergistic action of several of its components.
[1] Sotres J., Madsen J.B., Arnebrant T., Lee S. 2014. Adsorption and nanowear properties of bovine submaxillary mucin films on solid surfaces: Influence of solution pH and substrate hydrophobicity. J. Colloid Interface Sci. 428: 242-250.[2] Lindh L., Aroonsang W., Sotres J., Arnebrant T. 2014. Salivary Pellicles in Saliva, Monographs in Oral Science, 24: 30-39.
contact: [email protected]
mechAnicAl stAbilitY of mucin films: implicAtions on sAlivARY lubRicAtion
3.
figure 1. Load forces needed to completely remove salivary and mucin (BSM) films. Comparisons are shown for films formed on model hydrophilic and hydrophobic substrates, and for neutral and acidic pH conditions.
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4.
Periimplantitis is a biofilminduced destructive inflammatory process that, over time, results in loss of supporting bone around an osseointegrated dental implant. Inflammation is accompanied by an increased tissue proteolysis instrumental in driving bone destruction. Bacterial biofilms at periimplantitis sites have been reported to be dominated by Gramnegative anaerobic rods such as Fusobacterium, Porphyromonas, Prevotella and Tannerella with a proteolytic metabolism, as well as anaerobic streptococci. The hypothesis of this study was that a high pro-tease activity would be detected in samples of periimplant fluid from periimplantitis patients.
The microbial composition and level of protease activity in samples of periimplant biofluid from 25 implant patients with healthy support tissues and 25 patients with periimplantitis were compared. Microbial composition was studied using culture techniques and protease activity was determined using FITClabeled casein.
There was a pronounced interindividual variation in the microbial composition in both groups. Fusobacterium, Porphyromonas, Prevotella and Tannerella, anaerobic genera that all have been implicated in periimplant
disease, were found in both groups. However, the prevalence of Porphyromonas/Prevotella was significantly higher in the periimplantitis group than in the unaffected group. The most striking finding was that strictly anaerobic streptococci were almost exclusively found in the periimplantitis group (Fig 1)
The mean protease activity in the periimplant biofluid was about twice as high in the periimplantitis group as in the healthy group. The level of protease activity could not be correlated to the presence of any specific bacterial species. Instead, we propose that the presence of proteolytic bacterial phenotypes and high levels of protease activity in the periimplant biofluids may be a predictive factor for disease progression in periimplantitis. Further longitudinal studies are however required to determine whether assessment of protease activity could serve as a useful complement to other methods to identify patients at risk of progressive tissue destruction.
peRiimplAntitis, bActeRiAl species And pRoteolYtic phenotYpes
Project partners: J. Neilands, C. Wickström, B. Kinnby, J. R. Davies, J. Hall, B. Friberg and G. Svensäter
contact: gunnel.svensä[email protected]
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plAsminogen ActivAtoR inhibitoR tYpe 2 inhibits pRoteolYtic ActivitY of A multi-species bActeRiAl consoRtium
The plasminogen activator inhibitor PAI2 (SerpinB2) has been called the most enigmatic serine protease inhibitor (serpin) because it lacks an obvious endogenous target protease. Among cells producing PAI2 are macrophages and keratinocytes in skin and mucosa. PAI2 is rapidly induced by LPS. Thus, PAI2 may have evolved as a part of the defense against bacteria. The highest level of PAI2 has been found in the gingiva, an area characterized by the presence of a resident microflora that is important for oral health. An increased accumulation of bacteria leads to an inflammatory response, resulting in an increased flow of gingival fluid initiating an ecological shift. The results is an increased nutrient supply for bacteria dependent on peptides as their source of energy. In addition to degrading proteins for growth, bacterial proteases act as virulence factors, causing tissue damage and modulation of the host defense. No previous studies have demonstrated inhibition of bacterial proteases by PAI2. The aim of the present study was therefore to study possible interactions between PAI2 and proteases produced by bacteria in a multi species consortium resembling the resident microflora at subgingival sites.
A ninespecies bacterial consortium characteristic of periodontal pathology was grown for 7 days until a high proteolytic activity was expressed. The proteolytic activity of the culture supernatants was assayed using FITClabeled casein and that of the biofilm bacteria by confocal microscopy using fluorescent substrates. We found that PAI2 readily inhibits proteolytic activity in the supernatants from these bacteria (Fig 1) as well as surfaceassociated protease activity (Fig 2). This supports the hypothesis that PAI2 is present in epithelium as a defense barrier against proteaseproducing bacteria. This may be a future approach to treatment of destructive periodontal inflammation.
5.
Project partners: J. Neilands and B. Kinnby
contact: [email protected]
figure 2. protease activity visualized through blue fluorescence.
control +pai-2
figure 1.
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Plasminogen is a major plasma protein and the zymogen of the broad spectrum protease plasmin. Plasmin activity leads to tissue degradation, both directly and indirectly through activation of metalloproteinases. Infected tooth root canals, as a consequence of the inflammatory response and eventual necrosis, contain tissue fluid and blood components. These will coat the root canal walls and act as conditioning films that allow bacterial biofilms to grow and be a potential source for hematogenic spread of bacteria.
We investigated the effect of in vitro surface conditioning with human plasminogen on the initial adhesion of bacteria. Four bacterial species, L. salivarius, E. faecalis, A. naeslundii, and S. gordonii, isolated from dental root canals, and three other oral streptococci (S. anginosus, S. oralis, and S. sanguinis) were grown in albumin or plasminogencoated flow chambers and studied by confocal laser scanning microscopy using the cell viability staining LIVE/DEAD and 16S rRNA fluorescence in situ hybridization (FISH).
A. naeslundii, L. salivarius and in particular S. gordonii showed a higher initial adhesion to the plasminogencoated surfaces. E. faecalis did not show any preference for plasminogen (Fig 1). Fourspecies biofilms cultured for 96h showed that streptococci increased in proportion with time (Fig 2). Further experiments aimed at studying different streptococcal strains. All these adhered more to plasminogencoated surfaces than to albumincoated control surfaces (Fig 3). Plasminogen is thus an important plasma component for the initial adhesion of oral bacteria, in particular streptococci. This binding may contribute to their spread locally as well as to distant organs or tissues.
plAsminogen coAting incReAses initiAl Adhesion of oRAl bActeRiA to in vitRo suRfAces
6.
Project partners: B. Kinnby and L. E. Chávez de Paz
contact: [email protected]
figure 1.
figure 3.
figure 2.
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AmpeRometRic monitoRing of dRug penetRAtion thRough skin
7.
Transdermal drug delivery represents an attractive alternative to oral delivery especially when poor drug absorption or enzymatic degradation in the gastrointestinal tract or liver is a problem. From the first transdermal patch for treating motion sickness approved by the FDA in 1979, to the most recently approved patch for migraine (Sumatriptan), a total of more than 20 drugs with transdermal delivery have been commercialized so far.
The progress in developing transdermal drug delivery devices and methods to a great extend depends on the understanding properties of stratum corneum (SC). Stratum corneum (SC), the outermost thin layer of the skin, represents the major resistance to transdermal drug delivery. Characterizing the electrical properties of the skin in combination with penetration investigations can help the evaluation of the drug transport mechanisms across skin [1]. Penetration of compounds through SC or whole skin membranes is often studied by
exploiting flow through diffusion cells or Franz cells. However, dynamics of penetration is difficult to study using Franz cell based method. At the department of Biomedical Sciences we have developed amperomentric invitro methods for monitoring penetration of biologically active compounds through skin membranes, Fig. 1. Skin membrane is fixed on the top of chemically modified electrode and penetration of the appropriate compound is registered as electrode current. By using this method penetration dynamics of biologically active compounds possessing very different properties has been studied. Specifically, the electrochemical method was elaborated to monitor dynamics of penetration of quercetin, hydrogen peroxide and ascorbic acid. The methodology allows the study of penetration from solution as well as from pharmaceutical creams. From realtime measurements of electrode current, fluxes and diffusion coefficients of mentioned compounds in skin membranes have been estimated [2].
figure 1. (Left) photos of skin membranes-covered electrodes and schematic presentation of the electrode construction. (Right) Dynamics of penetration of different compounds through skin membrane recorded as alectrode current vs time dependencies. The penetration of quercetin and ascorbic acid was studied from soluti on, hydrogen peroxide from 1% containing peroxide cream.
[1] S. Björklund, T. Ruzgas, A. Nowacka, I. Dahi, D. Topgaard, E. Sparr, and J. Engblom. Skin membrane electrical impedance properties under the influence of a varying water gradient. Biophys. J. (2013), 104, 2639-2650.
[2] H. Gari, J. Rembiesa, I. Masilionis, N. Vreva, B. Svensson, T. Sund, H. Hansson, A. K. Morén, M. Sjöö, M. Wahlgren, J. Engblom, and T. Ruzgas. Amperometric In Vitro Monitoring of Penetration through Skin Membrane. Electroanalysis, (2015), 27, 111 – 117.
contact: [email protected]
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Humectants belong to a group of hydrophilic compounds frequently used in skin care products with the aim to diminish the clinical symptoms of skin dryness. The biochemical and biophysical mechanisms by which humectants interact with the skin barrier are far from fully understood. Increased understanding of such mechanisms can enhance the possibilities to tailor skin care products for various skin abnormalities.
We have focused on two low molecular weight humectants (glycerol and urea) and their interactions with water, as well as their effects on the barrier properties of the outermost layer of the skin, stratum corneum (SC). Addition of urea, glycerol or PEG1500 to aqueous formulations inevitably lowers the water activity of the formulation, which in turn affects the SC permeability when these formulations are applied on the skin. In contrast to low molecular weight humectants, like urea and glycerol, it was concluded that PEG1500 does not penetrate the skin barrier
due to size exclusion [1]. Moreover, it was shown that skin permeability of the model drug metronidazole decreases upon addition of PEG1500 to the formulation, while high skin permeability was maintained with addition of urea or glycerol despite a lower water activity of these formulations [2].
Confocal Raman microspectroscopy revealed formation of large water inclusions in fully hydrated SC after 24 h exposure to a pure buffer solution. Addition of urea was shown to promote the formation of these inclusions, while no inclusions could be detected in presence of PEG1500 (i.e. in less hydrated skin) [3]. A similar approach as used in vitro was employed in vivo to explore the effect of hydration and humectants on skin permeability. It was shown that the water activity of the applied formulation has a marked effect on the barrier properties and that urea and glycerol have the ability to improve skin hydration even at reduced water activity of the applied formulation [4].
effects of wAteR ActivitY And low moleculAR weight humectAnts on skin peRmeAbilitY And hYdRAtion dYnAmics
8.
[1] Björklund S, Engblom J, Thuresson K and Sparr E, A water gradient can be used to regulate drug transport across skin, J Control Release, 143: 2, (2010) 191-200
[2] Björklund S, Engblom J, Thuresson K and Sparr E, Glycerol and urea can be used to increase skin permeability in reduced hydration conditions, Eur J Pharm Sci, 50 (2013) 638–645
[3] Albèr C, Brandner BD, Björklund S, Billsten P, Corkery RW and Engblom J. Effects of water gradients and use of urea on skin ultrastructure evaluated by confocal Raman microspectroscopy. Biochimica et Biophysica Acta 1828 (2013) 2470–2478.
[4] Albèr C, BuraczewskaNorin I, Kocherbitov V, Saleem S, Lodén M and Engblom J. Effects of water activity and low molecular weight humectants on skin permeability and hydration dynamics – a doubleblind, randomized and controlled study. International Journal of Cosmetic Science 36 (2014), 412-418.
contact: [email protected]
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Mass spectrometry (MS) methods are in high demand in the field of clinical proteomics providing sensitive, reproducible and specific biomarker quantification. They represent the highthroughput option in clinical analysis limiting the false positive rate and enhancing the specificity of diagnostic assays. Such precise quantification tools need to be selective to analyze complex clinical samples where occurrence of high abundant protein and low biomarker expression limits the dynamic range. Improvements have been achieved by adopting highresolution MS and new strategies in sample cleanup such as immuno extraction techniques. Immunoaffinity coupled with MS has been demonstrated to be highly effective in biomarker determination.
In spite of the efficiency of immunobased methods, the generation of high quality antibodies is costly and timeconsuming which commonly hampers the development of analytical methods for clinical routine diagnosis. Indeed, methods which entirely avoid the use of antibodies are of growing importance in the bioanalysis field.
An alternative approach intended to mimic antibody specificity is represented by Molecularly Imprinted Polymers (MIP), commonly referred to as plastic antibodies. These artificial receptors, with affinity recognition sites able to recognize target molecules by a lock and key mechanism, have been used extensively as sample preparation tools in small molecule analysis. This contrasts with the few reports describing assays or enrichment of biomacromolecular targets. This can be ascribed to added complexity in generating the corresponding receptors in terms of template stability and scarcity, conformational matching and mass transfer limitations. In order to demonstrate and compare these two affinitybased enrichment techniques in massspectrometry based assays, we have here focused on assays for Pro Gastrin Releasing Peptide (ProGRP), a well established biomarker for the highly metastatic cancers form, small cell lung cancer (SCLC). Early detection is crucial for positive treatment outcomes and new diagnostic modalities for SCLC biomarker determination assume absolute importance.
AntibodY-fRee biomARkeR deteRminAtion: exploRing moleculARlY impRinted polYmeRs foR pRo-gAstRin ReleAsing peptide
9.
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[1] Qader, A. A.; Urraca, J.; Torsetnes, S. B.; Tönnesen, F.; Reubsaet, L.; Sellergren, B. Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide. J. Chromatogr. A. 2014, 1370, 56-62.
[2] Rossetti, C.; Qader, A. A.; Halvorsen, T. G.; Sellergren, B.; Reubsaet, L. Antibodyfree biomarker determination : exploring molecularly imprinted polymers for progastrin releasing Peptide. Anal. Chem. 2014, 86, 12291–12298
contact: [email protected]
Absolute quantification of ProGRP by MS was formerly investigated by the means of its signature peptide, NLLGLIEAK, a unique proteotypic nonapeptide detectable with high signal intensity. The aim of this work was to develop an alternative enrichment strategy in targeted proteomics exploring MIP based peptide capture. In contrast to MIPs targeting proteins the imprinting of peptides obviates the need for labile and expensive proteinaceous templates and is compatible with a wide range of solvents, monomers and elevated temperatures. Apart from synthetic ease this approach moreover allows a direct comparison with the increasingly popular methods relying on immunobased peptide capture. We have identified polymers prepared using the hydrophobic crosslinker divinilbenzene (DVB) and the functional monomer N(2aminoethyl) methacrylamide hydrochloride (EAMA) imprinted with N and C protected forms of the
signature peptide of ProGRP (NLLGLIEAK) to display particularly promising recognition properties [1]. Polymers prepared using this combination exhibited significant imprinting, yielding higher peptide recovery in the MIP extract when compared with the respective nonimprinted polymer. With this as starting point we have here developed novel peptide imprinted thin film composite beads and used them in conjunction with tandem mass spectrometry (MS/MS) in the bottomup workflow for absolute quantification [2]. A selectivity study was performed by evaluating both imprinted and nonimprinted polymers with respect to retention behavior of the target peptide (NLLGLIEAK) and other, nontarget, peptides. Ultimately the MIP’s potential in analysis of biological samples was demonstrated with respect to affinity and selectivity by applying the developed protocol to ProGRP fortified serum sample.
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The critical advances in the fuel cell and capacitors technologies enabled the development of a new kind of devices, hybrid electric power devices, viz. selfcharging supercapacitors or, in other words, chargestoring fuel cells. Scientifically a dualfunction electrode, viz. a discrete electrode concurrently manifesting electrocatalytic and chargestorage features, is disclosed.
Hybrid electric power device is a combination of an electrochemical capacitor and a fuel cell in a singular indivisible module. To create efficient devices, which can be miniaturised
even down to the nm scale and used for shorttime highcurrent and longtime lowcurrent practical applications, both elements (capacitive and electrocatalytic) were built from nano(bio)composite materials. Energy storing parts were built from the conducting organic polymer (e.g. polyaniline (PANI), polypyrrole (PPy))/carbon nanotube (CNT) nanocomposites immobilised on the surface of gold or graphite electrodes. Energy generating parts of hybrid electric power devices were based on nonbiological (organic (PANI) or inorganic (platinum (Pt)) or biological (redox enzymes) catalysts (see figure below) [1].
hYbRid electRic poweR devices foR simultAneous geneRAtion And stoRAge of electRic poweR
The work has been supported financially
by the Swedish Research Council (2013-
6006) and by the Russian Foundation for
Basic Research (13-04-12083 ofi_m).
[1] Pankratov, D., Blum, Z., Shleev, S. Hybrid electric power biodevices. ChemElectroChem. 2014, 1, 1798-1807.
[2] D. Pankratov, Z. Blum, D. Suyatin, V. Popov, S. Shleev. Selfcharging electrochemical biocapacitor, ChemElectroChem 2014, 2, 343-346.
[3] D. Pankratov, P. Falkman, Z. Blum, S. Shleev. A hybrid electric power device for simultaneous generation and storage of electric energy. Energy Environ. Sci. 2014, 7, 989-993.
contact: [email protected]
(Left) Self-charging biosupercapacior, (Right) Charge-storing fuel cell.
Operating in a pulsed power mode, hybrid electric power device s based on biological [2] and nonbiological [3] catalysts provided a maximum power density of 1.2 mW cm –2 and 1.4 mW cm –2 at 0.38 V, respectively. Thus, the power output was improved by a factor of around 100 in comparison to fuel cells based on the same catalysts.
Several niches for practical applications of these new devices can be envisaged, depending on the fuels and oxidants used. Hybrid electric power devices are able to act as supercapacitors, efficiently amassing large amounts of electric energy in a short time, and concurrently act as fuel cells, continuously supplying electric power, converted directly from chemical energy.
10.
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Degradable starch microspheres are used for drug delivery and also as a medical device for acceleration of wound healing by promoting hemostasis. In both applications hydrationdependent properties are essential for effective functioning of the microspheres. In a KKfunded project “Carbohydrate polymer – water interactions: sorption, porosity and rheology” we study effects of hydration on properties of starch microspheres and other carbohydrate systems.
In particular we investigated effects of crosslinks on properties of starch microparticles. Isothermal sorption calorimetry showed that crosslinks prevent crystallization of starch, which follows from the absence of crystallization peak, see figure below. The
isothermal glass transition is clearly seen in both materials, crosslinking shifts it to higher water contents. The absence of crystallization improves hygroscopicity of the particles, which is important for application as a medical device. The crystallization of acidhydrolyzed starch in the absence of crosslinks produces different crystalline structures depending on hydration levels, as is evident from synchrotron radiation Xray data obtained at MAXLAB. The Xray data treated using concept of fractals, also provided information on evolution of the microparticle surface and polymer chain properties as function of hydration. Based on results from several experimental methods we constructed temperaturecomposition phase diagrams of several starch materials.
hYdRAtion induced phenomenA in stARch micRopARticles
Carlstedt, J., Wojtasz, J., Fyhr, P., Kocherbitov, V. (2014) Hydration and the phase diagram of acid hydrolyzed potato starch. Carbohydrate Polymers, 112, 569–577
Wojtasz, J., Carlstedt, J., Fyhr, P., Kocherbitov, V. Hydration and swelling of amorphous crosslinked starch microspheres. Submitteda
contact: [email protected]
SEM: starch microspheres (dried) Sorption calorimetry: black curve – starch microspheres, red curve – acid hydrolyzed starch
11.
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Polyether ether ketone (PEEK) is today frequently used as a biomaterial in different medical operations due to its excellent mechanical and chemical properties. However, the untreated surface of PEEK is bioinert, hydrophobic and does not osseointegrate in its pure form. The aim of this study was to evaluate a nano CaP coated PEEK with
respect to enhanced osseointegration. The control implants were uncoated PEEK screws. Evaluation of material characteristics and evaluation of removal torque as a measure of osseointegration were performed. The results demonstrated a CaP coating did not influence the mechanical properties of PEEK but did enhance the osseointegration.
biomechAnicAl evAluAtion And suRfAce chARActeRizAtion of A nAno-modified suRfAce on peek implAnts: A studY in the RAbbit tibiA
Mean values and standard deviation of removal torque (Ncm) at both healing periods.
Histological section of one deceased rabbit after 3 weeks of healing
Johansson P., Jimbo R., Kjellin P., Currie F., Chrcanovic B., Wennerberg A.Johansson, Biomechanical evaluation and surface characterization of a nanomodified surface on PEEK implants: a study in the rabbit tibia, Int J Nanomedicine. 2014 Aug 14;9:3903-11
contact: [email protected]
12.
SEM images of coated pEEk at 80k magnification (A) and 10k magnification (B) and uncoated pEEk at 80k magnification (C) and 10k magnification (D)
A B
C D
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osteoconductive potentiAl of mesopoRous titAniA implAnt suRfAces loAded with mAg-nesium: An expeRimentAl studY in the RAbbit
background and aim: Mesoporous coatings enable incorporation of functional substances and sustainedly release them at the implant site. One bioactive substance that can be incorporated in mesoporous is magnesium, which is strongly involved in
bone metabolism and in osteoblast interaction. The aim of this project was to evaluate the effect of incorporation of magnesium into mesoporous coatings of oral implants on osseointegration.results of the animal study:
Published articles related to this project:
[1] Local release of magnesium from mesoporous TiO2 coatings stimulates the periimplant expression of osteogenic markers and improves osteoconductivity in vivo., Galli S, Naito Y, Karlsson J, He W, Miyamoto I, Xue Y, Andersson M, Mustafa K, Wennerberg A, Jimbo R. Acta Biomater. 2014 Dec;10(12):5193-201.
[2] Osteoconductive Potential of Mesoporous Titania Implant Surfaces Loaded with Magnesium: An Experimental Study in the Rabbit., Galli S, Naito Y, Karlsson J, He W, Andersson M, Wennerberg A, Jimbo R., Clin Implant Dent Relat Res. 2014
contact: [email protected], [email protected]
conclusion and future perspectives: It can be said that the effect of magnesium is significant to bone regeneration when appropriate carriers are selected. The results of the current project show that the magnesium enhanced osseointegration. The mechanisms
of the influence of magnesium and its longer effects needs to be investigated in future studies, however, the magnesium releasing biomaterial have the potential to improve the bone integrating medical devices.
13.
figure 1. Removal Torque testing (RTq) showed that the mesoporous coated implants loaded with magnesium presented significantly higher biomechanical bonding to the bone in a rabbit tibia model.
figure 2. Descriptive histological image of bone healing around a mesoporous implant loaded with magnesium. Active bone modeling and remodeling can be observed at the bone-implant interface.
figure 3. The real time RT-qpCR displayed the relative quantification of the expression of the panel of gene assays selected. There was a significant genetic effect of the mag-nesium loaded mesoporous implants. The significantly expressed bone modeling, remodeling related genes are noted with asterisks.
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LIST OF CENTER MEMBERS
1. mAnAgement And boARdssteeRing committee
Cristina Glad
Chair
CEO C glad Consulting AB
Thomas Arnebrant
Representative Faculty of Health and Society
Gunnel Svensäter
Representative Faculty of Odontology
Karl Obrant
Head of Research, Skåne university Hospital
Markus Johnsson
Senior Director, pharmaceutical
Development, Camurus AB
Ulf Brogren
CEO promimic AB
Peter Nordström
Senior project Manager, Medicon valley
Alliance
senioR ReseARcheRs foRum
Anna Holmberg
Chair
phD, Director
Alexei Iantchenko
professor
Ann Wennerberg
professor
Börje Sellergren
professor
Gunnel Svensäter
professor
Julia Davies
professor
Liselott Lindh
professor
Per Jönsson
professor
Sergey Shleev
professor
Tautgirdas Ruzgas
professor
Thomas Arnebrant
professor
Anette GjörloffWingren
Assoc. professor
Bertil Kinnby
Assoc. professor
During 2014, the Center comprised the following members.
2.
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peRmAnent AcAdemic stAff
Anna Holmberg, phD, Director
Alexei Iantchenko, professor
Ann Wennerberg, professor
Börje Sellergren, professor
Gunnel Svensäter, professor
Håkan Eriksson, professor
Julia Davies, professor
Lennart Ljunggren, professor
Liselott Lindh, professor
Per Jönsson, professor
Sergey Shleev, professor
Tautgirdas Ruzgas, professor
Thomas Arnebrant, professor
Anette GjörloffWingren, Assoc. professor
Bertil Kinnby, Assoc. professor
Christina Bjerkén, Assoc. professor
Claes Wickström, Assoc. professor
Krister Thuresson, Assoc. professor
(external)
Johan Engblom, Assoc. professor
Marité Cárdenas Gómez, Assoc. professor
Petri Gudmundsson, Assoc. professor
Ryo Jimbo, Assoc. professor
Vitaly Kocherbitov, Assoc. professor
Zoltan Blum, Assoc. professor
Gabriela Enggren, phD
Jakob Blomqvist, phD
Javier Sotres, phD
Jessica Neilands, phD
Lars Ohlsson, phD
Maria Stollenwerk, phD
Tove Sandberg, phD
Ravi Danielsson, phD
Yuanji Cheng, phD
3.
Christina Bjerkén
Assoc. professor
Claes Wickström
Assoc. professor
Johan Engblom
Assoc. professor
Vitaly Kocherbitov
Assoc. professor
Gabriela Enggren
phD
Maria Stollenwerk
phD
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technicAl And AdministRAtive stAff
Eva Nilsson, Administrative coordinator
Agnethe Henriksson, Technician
Madeleine Blomqvist, Technician
Ulrika Troedsson, phD, Technician
Peter Falkman, phD, Research engineer
phd students
Ali Alenezi
Anton Fagerström
Bruno Chrcanovic
Cathrine Albér
Celina Wierzbicka
Deyar Mahmood
Dmitrii Pankratov
Adjunct fAcultY
Justas Barauskas, phD
Senior scientist, Camurus AB
Tobias Halthur, phD
project Manager, CR Competence AB
4.
5.
junioR ReseARcheRs And post-docs
Anna Runnsjö, phD
Hala Ghandour Gari, phD
Jildiz Hamit Eminovski, phD
Jonas Carlstedt, phD
Grzegorz Lisak, phD from Åbo Akademi university, Finland (guest for one year)
Maria Falck Miniotis, phD
Pär Olsson, phD
Soma Ghosh, phD
Sudhirkumar Shinde, phD
Sebastian Björklund, phD
6.
Francesca Cecchinato
Jonas Anderud
Elena Gonzalez
Magnus Falk
Mariko Hayashi
Marjan Dorkhan
Mark Galat
7.
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Silvia Galli
Sing Yee Yeung
Tuerdi Maimaitiyili (enrolled at Lund univeristy)
Ulf Hejman (enrolled at Lund univeristy)
Vida Krikstolaityte (enrolled at vilnius
university, vilnius, Lithuania)
Zahra ElSchich
Michael Braian
Patrick Seumo (enrolled at university
of yaounde I, yaounde, Cameroon)
Peter Lamberg
Pär Johansson
Ramesh Chowdhary
Ricardo Trindade
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LIST OF RESEARCH ACTIVITIESThe research activities of the Center during 2014 are presented under the headlines below.
In addition Center members are partners in the EU FP7 Marie Curie Initial Training Network “PEPMIP – Robust affinity materials for applications in proteomics and diagnostics” (with Börje Sellergren as Coordinator), EU FP7 Marie Curie Initial Training Network “MagnIM” (Ann Wennerberg), the EU FP7 Marie Curie Initial Training Networks
“Biofuel cells from fundamentals to applications in bioelectrochemistry” (Sergey Shleev), the EU cooperation and mobility programme Erasmus Mundus EMBER (Tautgirdas Ruzgas) and the EU FP7 Marie Curie Initial Training Network Biopolymer Based Food Delivery Systems – BIBAFOODS (Marité Cárdenas Gómez).
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cell-suRfAce inteRActions
Mechanism of Toxicity of Aluminium
based Adjuvant (ABA) Nanomaterials
Funded by the British Medical Research
Council, MRC
pI (principal Investigator): Håkan Eriksson
Analysis of the Cellular and Molecular
Mechanisms of Inflammatory Cells
and Cancer Cells by Using Digital
Holography and Protein Technology
PhD project funded by Malmö
University
Project partner: Phase Holographic
Imaging AB
pI: Anette gjörloff-wingren
Innovative Microscopy and Digital
Holography for Studies of Biomarkers
of Eukaryotic Cells Integrated to
Biosensors and Monitoring Devices
Postdoc project funded by BRCB
Project partner: Phase Holographic
Imaging AB
pI: Anette gjörloff-wingren
Biological Responses NanoSize
Structures
Funded by the Knowledge Foundation
Project partner: Promimic
pI: Ann wennerberg
Hydrophilic and Hydrophobic Implant
Surfaces
Funded by industry
pI: Ann wennerberg
Advanced Surface Characterisation of
New Functional Biomaterials
Funded by the Swedish Research
Council
Project partners: Chalmers and
Dentsply
pI: Ann wennerberg
Development of a Hydrophilic
Nanostructured Abutment Surface to
Promote Soft Tissue Integration
Project partner: Straumann AG
pI: Ryo Jimbo
Imaging of cell surface glycans using
fluorogenic MIP nanoparticles
Funded by Malmö University
pI: Börje Sellergren, Anette gjörloff-wingren
Mechanical behaviour of biological cells
Funded by the Swedish Research
Council
Project partner: Björn Fallqvist, The
Royal Institute of Technology
pI: Martin kroon
1.
moleculAR tRAnspoRt phenomenA
Adjuvants for Products Used in Agriculture
Project partners: AkzoNobel Surface
Chemistry AB
Funded by the Knowledge Foundation,
Malmö University
pI: Johan Engblom
Humectants and Their Mechanisms
in Skin
Funded by the Knowledge Foundation
Project partners: SP AB, ACO Hud
Nordic AB, Eviderm Institute AB
pI: Johan Engblom
2.
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Annual Report 2014
3. moleculAR inteRActions At biointeRfAces
Development of Novel MultiFunctional
Salivary Substitutes for Dry Mouth
Syndrome Patients
Funded by the Swedish Laryng
Foundation, Camurus AB, Malmö
University
pI: Liselott Lindh
Screening of Different Surface Coatings
on Titanium including Different API’s
for Enhanced Wound Healing
Funded by the Swedish Laryng
Foundation and Malmö University
Project partner: NIOM, Oslo
pI: Liselott Lindh
Surface Modification with Natural
as well as Artificial Active Substances
of Dental Materials with the Aim to
Promote and Keep Good Oral Health
for the Spare Part Human
Funded by the Swedish Laryng
Foundation and Malmö University
Project partner: NIOM, Oslo
pI: Liselott Lindh
The Composition of Salivary Films
Formed on Surfaces with Different
Surface Wettability Including
Identification of Pellicle Proteins
Funded by the Swedish Laryng
Foundation and Malmö University
pI: Liselott Lindh
Carbohydrate Polymer Water
Interactions: Sorption, Porosity and
Rheology
Funded by the Knowledge Foundation
Project partner: Magle AB, Akzo Nobel
Pulp and Performance Chemicals AB
pI: vitaly kocherbitov
Development of HighResolution
Scanning QCMD Method for Studies
of Hydration of Biofilms
Funded by BRCB
pI: vitaly kocherbitov
Properties of Semifluorinated Alkanes
Funded by Novaliq GmbH
Project partner: Novaliq GmbH
pIs: Johan Engblom, vitaly kocherbitov
Viscoelastic Bioelectrocatalytic
Materials
Funded by the Swedish Research
Council
pIs: Tautgirdas Ruzgas, Thomas Arnebrant
Restorable and Adaptable Surfaces for
Molecular Recognition, Biosensing
and Evanescent Wave Microscopy of
Human Cells
Funded by the Swedish Research
Council
pI: Börje Sellergren
Flexible and Transparent Biofuel Cells
Funded by EU, Marie Curie
Project Partner: Obducat Technologies AB
pI: Sergey Shleev
SelfCharging Biosupercapacitors
Funded by the Swedish Research Council
pI: Sergey Shleev
Devices for NonInvasive Biomedical
Sensing, Monitoring or/and Delivery
Funded by the Knowledge Foundation
Project Partners: Galenica AB, Novosense,
Speximo AB, Bioglan AB
pI: Tautgirdas Ruzgas
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Biofilms – Research Center for Biointerfaces
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micRobiAl biofilms
Bacterial Acid Tolerance – a New
Target for Fluoridated Milk in Caries
Prevention
Funded by the Borrow Foundation, UK
pIs: gunnel Svensäter, Julia Davies
Novel Biomarkers for Oral Health Care
Funded by Region Skåne (OFRS)
pIs: gunel Svennsäter, Julia Davies, Claes
wickström
Oral Implants – Nanostructures for the
Promotion of Tissue Integration and
Prevention of Infections
Funded by the Knowledge Foundation
Project partners: Noble Biocare AB,
Promimic AB
pIs: gunnel Svensäter, Ann wennerberg
4.
Biomimetic Membranes for Revealing
the Function and Structure Relationship
of Membrane Bound Proteins and other
Biomolecules
Funded by the Swedish Research
Council
pI: Marité Cárdenas gómez
Dental Erosion: Novel Strategies against
an Escalating Problem
Funded by Crafoord Foundation
pI: Javier Sotres
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Biofilms – Research Center for Biointerfaces
Annual Report 2013
SCIENTIFIC INSTRUMENTATION AND EqUIpMENT
opticAl micRoscopY
Contact person: peter FalkmanA Nikon Optiphot EpiFluorescence
microscope equipped with polarizing
filters, phase contrast and a DSU1 digital
camera. A TransferMan NK2 micro
manipulator allows manipulation of sam
ples, such as mounting of AFM colloidal
probes, and a Linkam AnalysaLTS350
temperature controlled stage allows study
of temperatureinduced effects, e.g. lipid
phase transitions.
1.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
Contact person: Johan Engblom Three alternative thermostated in vitro
diffusion equipments are available in
house, two set ups with flow through
cells (15 + 7 cells) [R.L. Bronaugh & R.F.
Stewart, J. Pharm. Sci. 74 (1985) 64–67]
and 10 static Franz cells [Franz T, J.
Invest. Dermatol. 64 (1975) 190-195].
Solute diffusion over skin, oral mucosa,
nasal mucosa, nails, plant cuticle, as well
as synthetic membranes, has been studied
and assayed spectrophotometrically (on
line) or by HPLCUV. Electrical impedance
spectroscopy has been appended to the
Franz cells as a tool helping to explain
diffusion properties of solutes through
these membranes.
in vitRo diffusion equipment
swAxd
Contact persons: peter Falkman,
Johan EngblomSmall and Wide Angle XRay Scattering.
Kratky compact camera with line col
limation (slit focus), equipped with two
linear Mbraun detectors (small and wide
angle). Temperature controlled sample
stages (0–70°C or 25–300°C). Powder
(semisolid/solid) or capillary (liquid)
sample holders, as well as flowthrough
capillary sample holder.
dsc
Contact person: vitaly kocherbitovDifferential scanning calorimeter DSC1
from MettlerToledo equipped with an
intracooler TC100 and HSS8 sensor.
Temperature range: –90 to +550°C. The
calorimeter can be used to study solid,
soft and liquid substances. Typical sample
masses are 2–10 mg. The DSC can be run
in modulation regime to resolve reversing
and nonreversing heat effects.
5.
2.
3.
4.
ellipsometeR
Contact persons: peter Falkman,
Thomas ArnebrantAn automated Rudolph thin film ellipsom
eter (type 43603-200E, Rudolph Research,
USA) equipped with a xenon arc lamp
as a light source; an interference filter
with UV and infrared blocking (Melles
Griot, The Netherlands) for working at a
wavelength of 4429 Å. The experimental
setup is based on null ellipsometry in the
PCSA arrangement. The components of
the ellipsometer are controlled by means
of the Ellipso software that automatically
measures the ellipsometric angles Psi
and Delta allowing the calculation of the
thickness, refractive index, and adsorbed
mass of the growing films. To perform
experiments in liquid media a peristaltic
pump (Ole Dich Instruments) is used. The
temperature is controlled by means of a
Julabo 5B water bath. Data analysis is
performed with the Ellipsometry software
by Plamen Petrov.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
qcm-d
Contact person: Sebastian BjörklundThe QSense E4 quartz crystal micro
balance is equipped with the E4 measur
ing chamber that allows simultaneously
running a maximum of four experiments
in the four temperature controlled cells.
Changes in frequency and dissipation are
registered by means of the Eseries elec
tronic unit which is also responsible for
the temperature control. These changes
are related to the adsorbed wet mass and
the viscoelastic properties of the films
adsorbed onto the sensor. The QCMD
is also equipped with an electrochemis
try and an ellipsometry module enabling
the simultaneous measurement of the
electrochemical and optical properties
respectively of the adsorbed films. A four
channels IPC peristaltic pump (Ismatec).
Data analysis is performed with the
QTools software (QSense).
7.
6.
8.
e-sem
Contact persons: peter Falkman, Zoltan BlumScanning Electron Microscope, SEM, Zeiss
EVO LS10. Apart from standard modern
SEM features, i.e. 5 nm pointtopoint
resolution at high vacuum and fully con
ducting samples, the EVO LS10 also offers
environmental control (hence “EVO”). By
controlled water ioele introduction, with
or without the added benefits of a Peltier
Coolstage, fully hydrated samples can be
imaged with accurate sample temperature
control; owing to the high output LaB6
filament and the beam sleeve technology,
the loss in resolution is only tenfold. The
instrument is equipped with the appro
priate detectors and also an INCA EDX
microanalysis system.
spectRoscopic ellipsometeR uvisel hoRibA
Contact person: Marité Cárdenas gómezSpectroscopic ellipsometry is a simple
and fast optical and indirect technique
that offers the possibility to determine
the average thickness of the adsorbed
layer. Ellipsometry measures the ratio
of two values, which is the amplitude
ratio (Ψ=rp/ rs) and the phase difference
between light waves (Δ=Δp-Δs). These
two parameters are defined from the dif
ference in the reflection coefficients for
p and s polarizations and thus in the
optical properties of a material (extinction
co efficient and refractive index). We have
in house an UVISEL HORIBA spectro
scopic ellipsometer covering a wavelength
range of 200 – 820 nm.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
dls/zetA sizing sYstem
Contact person: peter FalkmanA combined Electrophoretic Light Scat
tering/Dynamic Light Scattering Zeta
potential/particle sizing system w ith a 5
MW 632.8 nm HeNe laser, two insert
able electrodes for high or low voltage,
covering possible electrical field strengths
between 0.25–250 V/cm, and an adjust
able temperature range of 4–100°C. The
instrument has further been fitted with a
Thermaltake Bigwater 760is liquid cool
ing system, to prevent cuvette fogging at
high temperatures.
goniometeR
Contact person: peter FalkmanKruss Goniometer DSA100 Drop shape
ioelect system for measurements of static
and dynamic contact angles. The goniom
eter is equipped with single dosing system
DS3200/3201. Temperature is controlled
in the range 5–90°C using TC30 measur
ing chamber. Humidity is controlled using
HC10 humidity chamber.
Afm
Contact persons: Javier Sotres,
Thomas ArnebrantA Multimode SPM 8 equipped with a
Nanoscope V control unit (Bruker AXS).
The SPM is also equipped with a PicoForce
Unit, a Heater Unit, and the ScanAsyst
and Peak Force Tapping softwares (Bruker
AXS). Furthermore, a digital oscilloscope
(Tektronix TDS 2022C) and a Nikon opti
cal microscope are coupled to the system.
impedAnce meAsuRing equipment
Contact person: Tautgirdas RuzgasFranz cells (Ø=0.90 cm, V=6 ml, PermeGear,
Hellertown, PA, USA) equipped with four
electrodes and connected to a potentiostat
from Ivium Technologies (Eindhoven, The
Netherlands) are used to study impedance
characteristics of membranes, including
skin, leafs, etc. Two platinum wires serve
as working and counter electrodes and two
Ag/AgCl/3M KCl electrodes from World
Precision Instruments (Sarasota, FL, USA)
are used as sensing and reference electrodes.
The equipment allows impedance measure
ments at frequencies up to 6 MHz.
11.
9.
10.
12.
dRop volume AppARAtus
Contact person: Tautgirdas RuzgasDrop Volume Tensiometer TVT 2 consists
of step motor driven syringe including a
needle for droplet formation, setup for opti
cal droplet falling registration, and software
to operate hardware and calculate surface
tension of liquid. This apparatus is used for
determination of surface tension of liquids.
13.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
RheometeR
Contact persons: vitaly kocherbitov,
peter FalkmanRheometer Bohlin CVO 100 Digital
equipped with Peltier Cylinder C25. The
rheometer system is suitable for research,
product development and quality control.
The rheometer is equipped with 20 mm
parallel plate, cone 4/40 and coaxial cyl
inders measuring systems.
soRption cAloRimeteR
Contact person: vitaly kocherbitovA doubletwin sorption calorimeter for
studies of hydration of powders and
soft materials. The channel diameter
is 28 mm. The calorimeter is equipped
with two sorption cells, each consist of a
sorption chamber (on top) and a vapori
zation chamber (bottom). The cambers
are connected by tubes with varying
diameters to control the ioele flow. The
double twin calorimeter is inserted into
an isothermal TAM 2277 calorimeter
from Thermometric equipped with a
nanoamplifier.
inteRfeRometeR
Contact persons: Ryo Jimbo, Ann
wennerbergWhite light microscopy, horizontal reso
lution 0.3 µm, vertical 0.1 nm, equipped
with a CCD camera, operating also in
phaseshift mode. The main application is
topographical characterisation on the µm
and subµm level. AFM Park Instrument,
surface characterization in the nm level.
Real time PCR Gene expression
pARticle electRophoResis
Contact person: Tautgirdas RuzgasParticle microelectrophoresis apparatus
Mark II (Rank Brothers, Cambridge,
UK) consists of flat electrophoretic cell
equipped with two platinum electrodes
and optical microscope. The apparatus can
be used to determine particle electropho
retic mobility in solution. The mobility can
be used to assess particle zeta potential and
surface charge density. Apparatus allows
measurements with particles bigger than
0.3 µm in diameter.
lAngmuiR suRfAce bAlAnce
Contact persons: Johan Engblom,
Marité Cárdenas gómezKSV 5000 is a programmable Langmuir
and LangmuirBlodgett instrument for
automated Langmuir film experiments and
for deposition of normal or alternating
multilayers onto solid substrates. Surface
pressure is measured using the Wilhelmy
plate method.
14.
15.
16.
17.
18.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
novAsinA lAbmAsteR-Aw
Contact person: Johan EngblomAn instrument for water activity measure
ments. Accuracy: 0.003 aw (0.3%RH),
range: 3 to 100%RH.
lc-ms
Contact person: Börje SellergrenZQ2000 MS system with 2795 LC and
2996 PDA. The Waters ZQ 2000 LCMS
System is based on the MicromassZQ
2000 Mass Selective Detector. This is a
robust single quadrupole mass spectro
meter, offering characteristic performance
for laboratories that require nominal
mass resolution for mass confirmation
appli cations. This system has a mass
range (amu) up to 2000 daltons. Easily
qualify and quantify small molecules in
a flash with the highly sensitive system.
Both inlet probes are quickly and easily
interchangeable without breaking vacuum.
Flow rates allowed are 5-1000 µl/min (ESI)
and 200–2000 µl/min (APCI).
lc –uv/dAd
Contact person: Börje SellergrenTwo Waters LCUV/DAD 2795/2996
(LCDAD) systems. This robust HPLC
apparatus allows flowrates from 50ul/
min to 5 ml/min for use with 2.1 mm
ID columns and larger. The autosampler
accepts standard 96/384 well plates with
programmable temperature control from
4 to 40°C. A heated column compartment
provides temperatures from 5 degrees
above ambient to 65°C. A range of LC
detectors, like a dual wavelength or PDA
UV detector is optionally available.
solution nmR spectRometeR
Contact person: Börje SellergrenNMR instrument Varian Mercury
400MHz with autosampler. Equipped
with 4 nucleus probe (C, H, P and F) and
probes adjustable to N, B, Si and O.
20.
19.
21.
22.
fluoRchem e
Contact person: Anette gjörloff wingrenHighperformance Western blot and gel
imaging. A selfcontained digital dark
room, compact design with stateoftheart
CCD optical technology, an integrated
computer and simplified touch screen
control. The FluorChem E is built with
imaging speeds and a dynamic range that
surpass film for low femtogram detection
of proteins and DNA in chemiluminescent,
colorimetric and UV fluorescent gels and
blots.
23.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
biofilm foRmAtion, including AdheRence, detAchment, populAtion diveRsitY And phenotYpic shift
micRobiAl diAgnostics; species And stRAin levels
Contact person: gunnel SvensäterAutoclaves
Anaerobic chamber
Fluorostar
Incubators
Spectrophotometer
PCR
Centrifuges
Contact person: gunnel SvensäterEpifluorescence microscope
Confocal laser scanning microscope
Flow cells with controlled continuous flow
Cell cultures
puRificAtion of mucins And pRoteins
Contact person: gunnel SvensäterFPLC
Preparative electrophores
Ultracentrifuge
pRoteomics, including RAdioActive lAbelling of pRoteins Contact person: gunnel SvensäterMultiphor
Twodimensional electrophoresis
Sonicator
identificAtion of pRoteins
Contact person: gunnel SvensäterImmunohistochemistry
Western blots
ELISA
24.
25.
26.
27.
28.
electRochemicAl equipment
Contact persons: Sergey Shleev,
Tuatgirdas Ruzgas6 potentiostats/galvanostats from Amel,
Autolab, BAS, and Ivium
Unique spectroelectrochemical system
(homemade 10 mL spectroelectrochemi
cal cell, spectrometer HR 4000 CGUV
NIR and light source DH2000 from
Ocean Optics)
Flowinjection analysis
29.
41
Biofilms – Research Center for Biointerfaces
Annual Report 2014
COLLABORATIVE pARTNERS OF THE CENTER
industRY collAboRAtoRs
ACO Hud Nordic AB
Acreo AB
A1M Pharma AB
Akzo Noble Pulp and Performance
Chemicals AB
Akzo Nobel Surface Chemistry AB
Amano Enzyme Inc., Japan
Bioglan AB
Biotage AB
Camurus AB
CapSenze HB
Corgio AB
CR Competence AB
Dentsply
Dräger AG, Germany
Enzymatica AB
Eviderm AB
Galenica AB
In vitro Planttech AB
Magle AB
Nares AB
Nobel Biocare AB
Novaliq GmbH, Germany
Novosense AB
Novozymes A/S, Denmark
Obducat Technologies AB
Orbital Systems AB
Promimic AB
Phase Holographic Imaging AB
Samsung, South Korea
SP AB
Speximo AB
Thermo Fischer, UK
Zelmic AB
1.
42
Biofilms – Research Center for Biointerfaces
Annual Report 2014
AcAdemic collAboRAtoRs
collAboRAtions with otheR univeRsities And ReseARch institutions
in sweden
Professor Anders Rosén, Cell Biology, university of Linköping
Professor Chris Anderson, Dermatology, university of Linköping
Professor em. Kåre Larsson, Camurus Lipid Research Foundation, Lund
Professor Emma Sparr, physical Chemistry, Lund university
Professor Fredrik Tiberg, Camurus AB, Lund
Professor Fredrik Winquist, Linköping university
Professor Gunnar Dahlén, Dept of Oral Microbiology, university of gothenburg
Professor Jens Schouenborg, Neuronano Research Center, Medical Faculty, Lund university
Professor Karin Schillén, physical Chemistry, Lund university
Professor Knut Irgum, uME, umeå
Professor Kristian Reisbeck, Dept of Medical Microbiology, Lund university
Professor Lars Montelius, Solid State physics, Lund university / INL- International Iberian Nanotechnology Laboratory, portugal
Professor Lo Gorton, Biochemistry, Lund university
Professor Marie Wahlgren, Food Technology, Faculty of Engineering, Lund university
Professor Mark Rutland, The Royal Institute of Technology and Sp AB, Stockholm
Professor Martin Andersson, Dept Applied Chemistry, Chalmers university of Technology, gothenburg
Professor Martin Malmsten, pharmacy, uppsala university
Professor Maud Langton, Swedish university of Agricultural Sciences, uppsala
Professor Olle Söderman, physical Chemistry, Lund university
Professor Pentti Tengvall, Dept Biomaterials, Sahlgrenska Academy, university of gothenburg
Professor Per Claesson, The Royal Institute of Technology and Sp, Stockholm
Professor Per Wollmer, Clinical Sciences, Lund university
Professor Staffan Nilsson, Lund university
2.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
Professor Thomas Laurell, Lund university
Professor Tomas Albrektsson, Dept Biomaterials, Sahlgrenska Academy, göteborg university
Professor Tommy Nylander, physical Chemistry, Lund university
Professor Torbjörn Bengtsson, Örebro university
Professor Viveka Alfredsson, physical Chemistry, Lund university
Assoc. Professor Malin Sjöö, Food Technology, Lund university
Assoc. Professor Marilyn Rayner, Food Technology, Lund university
Assoc. Professor Mats Eriksson, Linköping university
Assoc. Professor Ola Bergendorff, Dermatology, Lund university
Assoc. Professor Eva Blomberg, The Royal Institute of Technology and Sp AB, Stockholm
Assoc.Professor Peter Siesjö, Department of Clinical Sciences, BMC, Lund university
Per Rabe, Specialist Clinic for Oral Health Care, Hallands Läns Landsting, Halmstad
Doctor Andreas Sonesson, Lund university
Doctor Anna Westerlund, Odontology, gothenburg university
Doctor Annika Krona, The Swedish Institute for Food and Biotechnology, gothenburg
Doctor Bertil Friberg, Brånemark Clinic, gothenburg
Doctor Birgit Brandner, Sp kM AB, Stockholm
Doctor Hanna Wacklin, ESS, Lund
Doctor Jenny Liao Persson, Center for Molecular pathology, Lund university
Doctor Karina Persson, Inst. of Odontology, umeå university
Doctor Maria Lövenklev, The Swedish Institute for Food and Biotechnology, gothenburg
Doctor Marie Skepö, Dept of Theoretical Chemistry, Lund university
Doctor Robert Corkery, The Royal Institute of Technology, Stockholm
Doctor Sylvio Haas, MAX lab, Lund university
Doctor Tomás Plivelic, MAX lab, Lund university
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
inteRnAtionAl collAboRAtions
Professor Ben J. Boyd, Monash Institute of pharmaceutical Sciences, Monash university, Australia
Professor Birger Lindberg Møller, university of Copenhagen, Denmark
Professor Christopher Exley, keele university, uk
Professor David Beighton, guy’s kings and St Thomas’ Dental Institute, Joint Microbiology
Research unit, London, uk
Professor Deirdre Devine, Leeds Dental Institute, uk
Professor Dennis Cvitkovitch, Dept of Microbiology, university of Toronto, Canada
Professor Dietmar Haltrich, universität für Bodenkultur wien, Austria
Professor ECI Veerman, Department of periodontology and Oral Biochemistry, Academic
Centre for Dentistry Amsterdam (ACTA), The Netherlands
Professor Edmond Magner, university of Limerick, Ireland
Professor Gediminas Niaura, vilnius university, Lithuania
Professor Gordon Proctor, kings College, London, uk
Professor Jenny Emnéus, DTu Nanotech, Copenhagen, Denmark
Professor Jeannine Brady, Dept of Oral Biology, university of Florida, gainesville, uSA
Professor Jens Wiltfang, university of Essen, germany
Professor Jon E. Dahl, NIOM, Oslo, Norway
Professor Juozas Kulys, Inst. of Biochemistry, vilnius, Lithuania
Professor Kamal Mustafa, university of Bergen, Norway
Professor Katrin Markus, university of Bochum, germany
Professor Kell Mortensen, university of Copenhagen, Denmark
Professor Leon Rubesaet, uIO, Oslo, Norway
Professor Mark Herzberg, Department of Microbiology, university of Minnesota,
Minneapolis, uSA
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
Professor Markus Wenk, National university of Singapore, Singapore
Professor Ole Jensen, SDu, Odense, Denmark
Professor Peter Cormack, university of Strathclyde, uk
Professor Phil Bartlett, The university of Southampton, uk
Professor Phil Marsh, Health protection Agency, porton Down, uk
Professor Robert Baier, university of Buffalo, Industry/university Cooperative Research Center for Biosurfaces, Buffalo, uSA
Professor Takashe Sawase, Dept prosthodontics, university of Nagasaki, Japan
Professor Vitaliy Khutoryanskiy, university of Reading, uk
Professor Vladimir O. Popov, kurchatov NBIC Centre, Moscow, Russia
Professor Wolfgang Schuhmann, Ruhr-universität Bochum, germany
Research Assoc. Professor Anne Meyer, university of Buffalo, Industry/university Cooperative Research Center for Biosurfaces, Buffalo, uSA
Assoc. Professor Björn Hamberger, university of Copenhagen, Denmark
Assoc. Professor Claus J Løland, university of Copenhagen, Denmark
Assoc. Professor Floris Bikker, ACTA, The Netherlands
Assoc. Professor David Thornton, university of Manchester, uk
Assoc. Professor Jens Risbo, university of Copenhagen, Denmark
Assoc. Professor Lene Jørgensen, university of Copenhagen, Denmark
Assoc. Professor Marco van de Weert, university of Copenhagen, Denmark
Assoc. Professor Marie Ranson, School of Biological Sciences, Scientific Director
– Cancer, Illawarra Health and Medical Research Institute, university of wollongong,
wollongong, Australia
Assoc. Professor Tim TolkerNielsen, university of Copenhagen, Denmark
Assoc. Professor Thomas Günther Pomorski, university of Copenhagen, Denmark
46
Biofilms – Research Center for Biointerfaces
Annual Report 2014
Assoc. Professor Seunghwan Lee, Technical university of Denmark, Denmark
Doctor Antonio L. De Lacey, Consejo Superior de Investigaciones Científicas,
Bioelectrocatalysis group, Spain
Doctor Donald Leech, National university of Ireland, galway, Ireland
Doctor Humberto Schwartz, Sao paolo, Brasilien
Doctor JanTore Samuelsen, NIOM, Oslo, Norway
Doctor Magnus Röding, university of South Australia, Australia
Doctor Miguel Alcalde, Consejo Superior de Investigaciones Científicas, Applied
Biocatalysis group, Spain
Doctor Richard Campbel, Institute Laue Langevin, France
Doctor Paulo G. Coelho, Department of Biomimetics, New york university, uSA
Doctor Robert Barker, Institute Laue Langevin, France
Doctor Vida Krikstolaityte, DTu Nanotech, Copenhagen, Denmark
PhD student Nayab Chaudhury, kings College, London, uk
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
pHD THESES SUpERVISED BY CENTER MEMBERS
theses defended
theses in pRogRess
Magnus Falk
Malmö university (Supervisors Sergey
Shleev and Tautgirdas Ruzgas)
“Direct electron transfer based biofuel cells” Dissertation 2014-01-03
Deyar Mahmood
Malmö university (Supervisors: Ann
wennerberg, per vult von Steyern)
“On core and bi-layered all-ceramic
fixed dental prostheses, design and
mechanical properties : studies on stabilized
zirconiumdioxide”Dissertation 2014-03-18
Marjan Dorkhan
Malmö university (Supervisors Julia Davies,
gunnel Svensäter, Ann wennerberg):
“Activities of microbial biofilms on bioactive
implant surfaces” Dissertation 2014-04-04
Ramesh Chowdhart
Malmö university (supervisors Ann
wennerberg, Ryo Jimbo, Liselott Lindh)
“On efficacy of implant thread design for
bone stimulation” Dissertation 2014-05-21
Anton Fagerström
Malmö university (Supervisors Johan
Engblom, vitaly kocherbitov and karin
Bergström (AkzoNobel))
“Bioavailability of active ingredients used in
agriculture”Dissertation 2014-05-28
Peter Lamberg
Malmö university (Supervisors: Tautgirdas
Ruzgas, Thomas Arnebrant, Sergey Shleev)
“Design and characterization of direct elec-
tron transfer based biofuel cells including
tests in cell cultures”Dissertation 2014-09-15
Vida Krikstolaityte
vilnius university (Supervisors: Arunas
Ramanavicius at vilnius university,
Tautgirdas Ruzgas)
“Design and Characterisation of the
electrodes of enzymatic biofuel cells”Dissertation 2014-10-06
Tuerdi Maymaytilli
Faculty of Engineering, Lund university
(Supervisors: per Ståhle, Christina Bjerkén)
Influence of plastic deformation on the
formation and growth of embritteling metal
hydride’s” Start: 2009
Mariko Hayashi
Malmö university (Supervisors: Liselott
Lindh, Ann wennerberg, Ryo Jimbo)
“Biological Responses to Amphiphilic
Nano-size Structures” Start: 2010
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
Cathrine Albér
Malmö university (Supervisors:
Johan Engblom, vitaly kocherbitov,
Marie Lodén, Robert Corkery)
“Humectants and their effects on skin barrier
function – a mechanistic approach” Start: 2010
Zahra ElSchich
Malmö university (Supervisors:
Anette gjörloff wingren, Anna ketelsen,
gunilla Nordin Fredrikson)
“Microscopy and digital holography for
real-time applications and imaging: studying
the cellular and molecular mechanisms of
inflammatory cells and cancer cells”Start: 2011
Celina Wierzbicka
Malmö university (Supervisor:
Börje Sellergren, Thomas Arnebrant)
“New approaches to molecularly imprinted
micro- and nano- particles for post-
translationally modified proteins and protein
fragments” Start: 2012
Dmitrii Pankratov
Malmö university (Supervisors:
Sergey Shleev and Zoltan Blum)
“Function of bioelectronic devices in “extra
vivo” situations” Start: 2012
Mark Galat
Malmö university (Supervisor:
Börje Sellergren, Thomas Arnebrant)
“Development and characterisation of
molecularly imprinted polymer receptors
targeting pathogenic and biomarker
peptides related to neurodegenerative
diseases” Start: 2013
Sing Yee Yeung
Malmö university (Supervisors:
Börje Sellergren, Thomas Arnebrant)
“Restorable and adaptable surfaces for
molecular recognition, biosensing and eva-
nescent wave microscopy of human cells” Start: 2013
Elena Gonzalez
Malmö university (Supervisors:
Sergey Shleev and Lars Montelius)
“Flexible and transparent biofuel cells” Start: 2014
Pär Johansson
Malmö university (Supervisors: Ann
wennerberg, Ryo Jimbo, per kjellin)
“On Foreign Body Reactions Around Oral
Implants: The Role of the Inflammation
Balance in Osseointegration”Start: 2013
Silvia Galli
Malmö university (Supervisors: Ryo Jimbo,
Ann wennerberg)
“On the effect of magnesium incorporated
biomaterials for bone regeneration”Start: 2012
Francesca Cecchinato
Malmö university (Supervisors: Ryo Jimbo,
Ann wennerberg)
“Characterisation of magnesium alloys for
oral & maxillofacial applications”Start: 2012
Jonas Anderud
Malmö university (Supervisors:
Ann wennerberg, Sten Isaksson,
peter Abrahamsson)
“guided bone regeneration using ceramic
spacemaintaining devices”Start: 2011
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Biofilms – Research Center for Biointerfaces
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Michael Braian
Malmö university (Supervisor:
Ann wennerberg)
“On the influence of misfit on implant
supraconstructions”Start: 2008
Ricardo Trindade
Malmö university (Supervisors:
Ann wennerberg, Ryo Jimbo,
Tomas Albrektsson, pentti Tengvall)
“On Foreign Body Reactions Around Oral
Implants: The Role of the Inflammation
Balance in Osseointegration”Start: 2013
Ali Alenezi
Malmö university (supervisors Ryo Jimbo,
Ann wennerberg, Martin Andersson)
“On enhancement of bone regeneration
using drug delivery agents”Start: 2014
Bruno Chrcanovic
Malmö university Malmö u(supervisors
Tomas Albrektsson, Ann wennerberg,
Björn klinge)
“On risk factors for implant failure”Start: 2013
Patrick Seumo
university of yaoundé I, yaounde,
Camoroon (Supervisors: Ngameni
Emmanuel at university of yaoundé I,
Nanseu Njiki Charles peguy at university
of yaoundé I, Tautgirdas Ruzgas)
“Application of nanoparticles
in electroanalysis”Start: 2010
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LIST OF pUBLICATIONS AT THE CENTER FROM 2014
Lamberg P, Shleev S, Ludwig R, Arnebrant T, Ruzgas T. Performance of enzymatic fuel cell in cell culture. 2014, Biosens Bioelectron. 55:168-73.
Krikstolaityte V, Barrantes A, Ramanavicius A, Arnebrant T, Shleev S, Ruzgas T. Bioelectrocatalytic reduction of oxygen at gold nanoparticles modified with laccase. 2014, Bioelectrochemistry, 95:1-6.
Dagys, M; Lamberg, P; Shleev, S; Niaura, G; Bachmatova, I; Marcinkeviciene, L; Meskys, R; Kulys, J; Arnebrant, T; Ruzgas, T. Comparison of bioelectrocatalysis at Trichaptum abietinum and Trametes hirsuta laccase modified electrodes. 2014, Electrochim. Acta, 130, 141-147.
Tchekwagep, PMS; NanseuNjiki, CP; Ngameni, E; Arnebrant, T; Ruzgas, T. Quantification of BSA concentration by using Ag electrochemistry in chloride solution: extension of the linear range. 2014, Electrochim. Acta, 135: 351-355.
Krikstolaityte, V; Lamberg, P; Toscano, MD; Silow, M; EicherLorka, O; Ramanavicius, A; Niaura, G; Abariute, L; Ruzgas, T; Shleev, S. Mediatorless Carbohydrate/Oxygen Biofuel Cells with Improved Cellobiose Dehydrogenase Based Bioanode. 2014 Fuel cells, 14: 792-800.
Makaraviciute, A; Ruzgas, T; Ramanavicius, A; Ramanaviciene, A. Antibody fragment immobilization on planar gold and gold nanoparticle modified quartz crystal microbalance with dissipation sensor surfaces for immunosensor applications. 2014, Analytical methods, 6: 2134-2140.
Makaraviciute, A, Ruzgas, T, Ramanavicius, A, Ramanaviciene, A. A QCMD Study of reduced antibody fragments immobilized on planar gold and gold nanoparticle modified sensor surfaces. 2014 Key Eng. Materials, 605: 340-343.
Fagerström A, Kocherbitov V, Westby P, Bergsström K, Arnebrant T and Engblom J. Surfactant softening of plant leaf cuticle model wax – a Differential Scanning Calorimetry (DSC) and Quartz Crystal Microbalance with Dissipation (QCMD) study. J Colloid Interface Sci 426 (2014) 22-30.
Gari H, Rembiesa J, Masilionis I, Vreva N, Svensson B, Sund T, Hansson H, Morén AK, Sjöö M, Wahlgren M, Engblom J and Ruzgas T. Amperometric In Vitro Monitoring of Penetration through Skin Membrane. Electroanalysis. doi: 10.1002/elan.201400426.
Albèr C, BuraczewskaNorin I, Kocherbitov V, Saleem S, Lodèn M and Engblom J. Effects of water activity and low molecular weight humectants on skin permeability and hydration dynamics – a doubleblind, randomized and controlled study. International Journal of Cosmetic Science, 2014, 1–7.
Berghaus, M.; Mohammadi, R.; Sellergren, B. Productive encounter: molecularly imprinted nanoparticles prepared using magnetic templates. Chem. Commun. 2014, 50, 8993-8996.
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Kecili, R.; Billing, J.; Nivhede, D.; Sellergren, B.; Rees, A.; Yilmaz, E. Fast identification of selective resins for removal of genotoxic aminopyridine impurities via screening of molecularly imprinted polymer libraries. Journal of Chromatography A. 2014, 1339, 65-72.
Narayanaswamy, P.; Shinde, S.; Sulc, R.; Kraut, R.; Staples, G.; Thiam, C. H.; Grimm, R.; Sellergren, B.; Torta, F.; Wenk, M. R. Lipidomic “Deep Profiling” : An Enhanced Workflow to Reveal New Molecular Species of Signaling Lipids. Anal. Chem. 2014, 86, 3043-3047.
Rossetti, C.; Qader, A. A.; Halvorsen, T. G.; Sellergren, B.; Reubsaet, L. Antibodyfree biomarker determination : exploring molecularly imprinted polymers for progastrin releasing Peptide. Anal. Chem. 2014, 86, 12291–12298.
Kadhirvel, P.; Azenha, M.; Schillinger, E.; Halhalli, M.; Silva, A.; Sellergren, B. Recognitive nanothinfilm composite beads for the enantiomeric resolution of the metastatic breast cancer drug aminoglutethimide. J. Chromatogr. A. 2014, 1358, 93-101.
Qader, A. A.; Urraca, J.; Torsetnes, S. B.; Tönnesen, F.; Reubsaet, L.; Sellergren, B. Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide. J. Chromatogr. A. 2014, 1370, 56-62.
Kadhirvel, P.; Azenha, M.; Silva, F.; Sellergren, B. Chromatographycally efficient microspherical composites of molecularly imprinted xerogels deposited inside mesoporous silica. J. Chromatogr. A. 2014, 1355, 158-163.
Nematollahzadeh, A.; Lindemann, P.; Sun, W.; Stute, J.; Lütkemeyer, D.; Sellergren, B. Robust and selective nano cavities for protein separation : An interpenetrating polymer network modified hierarchically protein imprinted hydrogel. J. Chromatogr. A. 2014, 1345, 154-163.
Azenha, M.; Schillinger, E.; Sanmartin, E.; Regueiras, M T.; Silva, F.; Sellergren, B. Vaporphase testing of the memoryeffects in benzene and tolueneimprinted polymers conditioned at elevated temperature. Anal. Chim. Acta 2014, 802, 40-45.
Pankratov, D., Blum, Z., Suyatin, D., Popov, V., Shleev, S. Selfcharging electrochemical biocapacitor. ChemElectroChem 2014, 2, 343-346.
Pankratov, D., Falkman, P., Blum, Z., Shleev, S. Hybrid electric power device for simultaneous generation and storage of electric energy. Energy Environ. Sci. 2014, 7, 989-993.
Pankratov, D., Sotres, J., Barrantes, A., Arnebrant, T., Shleev, S. Interfacial behavior and activity of laccase and bilirubin oxidase on bare gold surfaces. Langmuir 2014, 30, 2943-2951.
Blum, Z., Pankratov, D., Shleev, S. Powering electronic contact lenses: current achievements, challenges, and perspectives. Expert Rev, Ophthalmol, 2014, 9, 269-273.
Pankratov, D., Blum, Z., Shleev, S. Hybrid electric power biodevices. ChemElectroChem. 2014, 1, 1798-1807.
Falk, M., Pankratov, D., Lindh, L., Arnebrant, T., Shleev, S. Miniature direct electron transfer based enzymatic fuel cell operating in human sweat and saliva. Fuel Cells, 2014, 14, 1050-1056.
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Pankratov, D., Sundberg, R., Suyatin, D., Sotres, J., Barrantes, A., Ruzgas, T., Maximov, I., Montelius, L., Shleev, S. The influence of nanoparticles on enzymatic bioelectrocatalysis. RSC Advances, 2014, 4, 38164-38168.
Pita, M., Mate, D., GonzalezPerez, D., Shleev, S., Fernandez, V., Alcalde, M., De Lacey, A. Bioelectrochemical oxidation of water. J. Am. Chem. Soc. 2014, 136, 5892-5895.
Falk, M., Alcalde, M., Bartlett, P.N., De Lacey, A.L., Gorton, L., GutierrezSanchez, C., Haddad, R., Kilburn, J., Leech, D., Ludwig, R., Magner, E., Mate, D., Conghaile, P., Ortiz, R., Pita, M., Poller, S., Ruzgas, T., SalajKosla, U., Schuhmann, W., Sebelius, F., Shao, M., Stoica, L., Sygmund, L., Tilly, J., Toscano, M., Vivekananthan, J., Wright, E., Shleev, S. Selfpowered wireless carbohydrate/oxygen sensitive biodevice based on radio signal transmission. PLoS One, 2014, 9, e109104/1-e109104/9.
Aroonsang W., Sotres J., ElSchich Z., Arnebrant T., Lindh L. Influence of substratum hydrophobicity on salivary pellicles: organization or composition? Biofouling, 2014, 30:1123-32.
Sotres J., Madsen J.B., Arnebrant T., Lee S. Adsorption and nanowear properties of bovine submaxillary mucin films on solid surfaces: Influence of solution pH and substrate hydrophobicity. J. Colloid Interface Sci. 2014, 428:242-250.
Sotres J., Barrantes A., Lindh L., Arnebrant T. 2014. Strategies for a direct characterization of phosphoproteins on hydroxyapatite surfaces. Karger 2014; Caries Research; 2 98-110.
Lindh, L., Aroonsang, W., Sotres, J., Arnebrant, T. Salivary pellicles. Eds. Ligtenberg AJM, Veerman ECI (eds): Saliva. Monogr Oral Sci. Basel, Karger, 2014, vol 24, pp 30–39. DOI: 10.1159/000358782.
T.K Lind, M. Cárdenas, H.P. Wacklin. Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature. Langmuir, 2014, 30, 7259.
S. Mølgaard, M. Henrikson, M. Cárdenas, A. Svagan. Cellulosenanofiber/ polygalacturonic acid coatings with high oxygen barrier and targeted release properties. Carbohydrate Polymers 2014, 114, 179.
Carlstedt J., Wojtasz J., Fyhr P., Kocherbitov V. Hydration and the phase diagram of acid hydrolyzed potato starch. Carbohydrate Polymers 2014; 112, 569–577.
Barauskas, J; Christerson, L; Wadsater, M; Lindstrom, F; Lindqvist, AK; Tiberg, F. Bioadhesive Lipid Compositions : SelfAssembly Structures, Functionality, and Medical Applications. Mol. Pharmaceutics, 2014, 11 (3), pp 895–903. DOI: 10.1021/mp400552.
Kapp SJ, Larsson I, Van De Weert M, Cárdenas M, Jorgensen L. Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces. J Pharm Sci. 2015 Feb; 104(2):593-601. Doi: 10.1002/jps.24265. Epub 2014 Dec 1.
Osipov E, Polyakov K, Kittl R, Shleev S, Dorovatovsky P, Tikhonova T, Hann S, Ludwig R, Popov V. Effect of the L499M mutation of the ascomycetous Botrytis aclada laccase on redox potential and catalytic properties. Acta Crystallogr D Biol Crystallogr. 2014 Nov; 70 (Pt 11):2913-23. Doi: 10.1107/S1399004714020380. Epub 2014 Oct 23.
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Wadsäter M, Barauskas J, Nylander T, and Tiberg F. Formation of Highly Structured Cubic Micellar Lipid Nanoparticles of Soy Phosphatidylcholine and Glycerol Dioleate and Their Degradation by Triacylglycerol Lipase. ACS Appl. Mater. Interfaces, 2014, 6 (10), pp 7063–7069. DOI: 10.1021/am501489e. Publication Date (Web): April 29, 2014.
D. V. Pankratov, Y. S. Zeifman, A. V. Dudareva, G. K. Pankratova, M. E. Khlupova, Y. M. Parunova, D. N. Zajtsev, N. F. Bashirova, V. O. Popov, and S. V. Shleev. Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase. Acta Naturae. 2014 JanMar; 6(1): 102–106.
Hansson M, Brandt SS, Lindström J, Gudmundsson P, Jujić A, Malmgren A, Cheng Y. Segmentation of Bmode cardiac ultrasound data by Bayesian Probability Maps Med Image Anal. 2014 Oct; 18(7):1184-99. Doi: 10.1016/j.media.2014.06.004. Epub 2014 Jun 26.
Conlon B, Geoghegan J, Waters E, McCarthy H, Rowe S, Davies JR, Schaeffer C, Foster T, Fey P, O’Gara J (2014). A role for the A domain of unprocessed accumulation associated protein (Aap) in the attachment phase of the Staphylococcus epidermidis biofilm phenotype. J Bacteriol 196:4268-75.
Dorkhan M, Hall J, Uvdal P, Sandell A, Svensäter G, Davies JR. Crystalline anataserich titanium can reduce adherence of oral streptococci. Biofouling 2014; 30:751-759.
Dorkhan M, YucelLindberg T, Hall J, Svensäter G, Davies JR. Adherence of human oral keratinocytes and gingival fibroblasts to nanostructured titanium surfaces. BMC Oral Health 2014; 14:75.
Neilands J, Troedsson U, Sjödin T, Davies JR. The effects of delmopinol and fluoride on acid adaptation and acid production in dental plaque biofilms. Arch Oral Biol 2014; 59:318-323
Svensson D, Westman J, Wickström C, Jönsson D, Herwald H, Nilsson BO. The human endogenous peptide p33 inhibits detrimental effects of LL37 on osteoblast viability J Periodont 2014; doi:10.1111/jre.12184.
Barrantes, A., Arnebrant, T., Lindh, L. Characteristics of saliva films adsorbed onto dental materials studied by QCMD. Colloids and Surfaces A: Physicochem Eng Aspects, 442: 56– 62, 2014 (DOI:http://dx.doi.org/10.1016/j.colsurfa.2013.05.054).
Falck Miniotis, Maria; Mukwaya Anthonny; Gjörloff Wingren Anette. Digital holographic microscopy for noninvasive monitoring of cell cycle arrest in L929 cells. PLoS One 2014; 9:e106546.
Mold, M., Eriksson, H., Siesjö, P., Darabi, A., Shardlow, E. and Exley. C. Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP1 cell line. Scientific Reports, 4, 6287-6293, 2014.
Tovar N, Jimbo R, Marin C, Witek L, Suzuki M, Bonfante EA, Coelho PG. Bone regeneration around implants placed in fresh extraction sockets covered with a duallayer PTFE/collagen membrane: an experimental study in dogs. Int J Periodontics Restorative Dent 2014; 34(6):849-855.
Naito Y, Jimbo R, Bryington MS, Vandeweghe S, Chrcanovic BR, Tovar N, Ichikawa T, Paulo G C, Wennerberg A. The influence of 1α.25dihydroxyvitamin d3 coating on implant osseointegration in the rabbit tibia. J Oral Maxillofac Res 2014; 5(3):e3.
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Anderud J, Jimbo R, Abrahamsson P, Isaksson SG, Adolfsson E, Malmström J, Kozai Y, Hallmer F, Wennerberg A. Guided bone augmentation using a ceramic spacemaintaining device. Oral Surg Oral Med Oral Pathol Oral Radiol 2014; 118(5):532-538.
Tovar N, Jimbo R, Witek L, Anchieta R, Yoo D, Manne L, Machado L, Gangolli R, Coelho PG. The physicochemical characterization and in vivo response of micro/ nanoporous bioactive ceramic particulate bone graft materials. Mater Sci Eng C Mater Biol Appl 2014; 43:472-480.
Galli S, Naito Y, Karlsson J, He W, Miyamoto I, Xue Y, Andersson M, Mustafa K, Wennerberg A, Jimbo R .Local release of magnesium from mesoporous TiO2 coatings stimulates the periimplant expression of osteogenic markers and improves osteoconductivity in vivo. Acta Biomater 2014; 10(12):5193-5201.
Johansson P, Jimbo R, Kjellin P, Currie F, Chrcanovic BR, Wennerberg A. Biomechanical evaluation and surface characterization of a nanomodified surface on PEEK implants: a study in the rabbit tibia. Int J Nanomedicine 2014; 9:3903-3911.
Barkarmo S, Andersson M, Currie F, Kjellin P, Jimbo R, Johansson CB, Stenport V. Enhanced bone healing around nanohydroxyapatitecoated polyetheretherketone implants: An experimental study in rabbit bone. J Biomater Appl 2014; 29(5):737-747.
Jimbo R, Janal MN, Marin C, Giro G, Tovar N, Coelho PG. The effect of implant diameter on osseointegration utilizing simplified drilling protocols. Clin Oral Implants Res 2014; 25(11):1295-1300.
Coelho PG, Jimbo R. Osseointegration of metallic devices: current trends based on implant hardware design. Arch Biochem Biophys 2014; 561:99-108.
Sumi T, Takeshita K, Takeichi T, Coelho PG, Jimbo R. Patientspecific gingivacolored abutments: a case series. Int J Periodontics Restorative Dent 2014; 34(4):469-475.
Zambuzzi WF, Bonfante EA, Jimbo R, Hayashi M, Andersson M, Alves G, Takamori ER, Beltrão PJ, Coelho PG, Granjeiro JM. Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations. PLoS One 2014; 9(7):e95662.
Cuellar JM, Yoo A, Tovar N, Coelho PG, Jimbo R, Vandeweghe S, Kirsch T, Quirno M, Errico TJ. The effects of Amicar and TXA on lumbar spine fusion in an animal model. Spine 2014; 39(19):E1132-1137.
Papia E, Jimbo R, Chrcanovic BR, Andersson M, Vult von Steyern P. Surface structure and mechanical properties of impactionmodified YTZP. Dent Mater 2014; 30(8):808-816.
Coelho PG, Takayama T, Yoo D, Jimbo R, Karunagaran S, Tovar N, Janal MN, Yamano S. Nanometerscale features on micrometerscale surface texturing: a bone histological, gene expression, and nanomechanical study. Bone 2014; 65:25-32.
Jimbo R, Tovar N, Anchieta RB, Machado LS, Marin C, Teixeira HS, Coelho PG. The combined effects of undersized drilling and implant macrogeometry on bone healing around dental implants: an experimental study. Int J Oral Maxillofac Surg 2014; 43(10):1269-1275.
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Jimbo R, Andersson M, Vandeweghe S. Nano in implant dentistry. Int J Dent 2014; 314819.
Jimbo R, Tovar N, Marin C, Teixeira HS, Anchieta RB, Silveira LM, Janal MN, Shibli JA, Coelho PG. The impact of a modified cutting flute implant design on osseointegration. Int J Oral Maxillofac Surg 2014; 43(7):883-888.
Breding K, Jimbo R, Hayashi M, Xue Y, Mustafa K, Andersson M. The effect of hydroxyapatite nanocrystals on osseointegration of titanium implants: an in vivo rabbit study. Int J Dent 2014; 171305.
Jimbo R, Tovar N, Janal MN, Mousa R, Marin C, Yoo D, Teixeira HS, Anchieta RB, Bonfante EA, Konishi A, Takeda K, Kurihara H, Coelho PG. The effect of brainderived neurotrophic factor on periodontal furcation defects. PLoS One 2014; 9(1):e84845.
Cecchinato F, Xue Y, Karlsson J, He W, Wennerberg A, Mustafa K, Andersson M, Jimbo R. In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating. J Biomed Mater Res A 2014; 102(11):3862-3871.
Naito Y, Terukina T, Galli S, Kozai Y, Vandeweghe S, Tagami T, Ozeki T, Ichikawa T, Coelho PG, Jimbo R. The effect of simvastatinloaded polymeric microspheres in a critical size bone defect in the rabbit calvaria. Int J Pharm 2014; 461(1-2):157-162.
Tovar N, Jimbo R, Gangolli R, Witek L, Lorenzoni F, Marin C, Manne L, PerezTroisi L, Baldassarri M, Coelho PG. Modification of xenogeneic graft materials for improved release of P15 peptides in a calvarium defect model. J Craniofac Surg 2014; 25(1):70-76.
Coelho PG, Teixeira HS, Marin C, Witek L, Tovar N, Janal MN, Jimbo R. The in vivo effect of P15 coating on early osseointegration. J Biomed Mater Res B Appl Biomater 2014; 102(3):430-440.
Tovar N, Jimbo R, Gangolli R, Perez L, Manne L, Yoo D, Lorenzoni F, Witek L, Coelho PG. Evaluation of bone response to various anorganic bovine bone xenografts: an experimental calvaria defect study. Int J Oral Maxillofac Surg 2014; 43(2):251-260.
Yoo D, Tovar N, Jimbo R, Marin C, Anchieta RB, Machado LS, Montclare J, Guastaldi FP, Janal MN, Coelho PG. Increased osseointegration effect of bone morphogenetic protein 2 on dental implants: an in vivo study. J Biomed Mater Res A 2014; 102(6):1921-1927.
Wennerberg A, Jimbo R, Stübinger S, Obrecht M, Dard M, Berner S. Nanostructures and hydrophilicity influence osseointegration: a biomechanical study in the rabbit tibia. Clin Oral Implants Res 2014; 25(9):1041-1050.
Chowdhary R, Jimbo R, Thomsen CS, Carlsson L, Wennerberg A. The osseointegration stimulatory effect of macrogeometrymodified implants: a study in the rabbit. Clin Oral Implants Res 2014; 25(9):1051-1055.
Jimbo R, Tovar N, Yoo DY, Janal MN, Anchieta RB, Coelho PG. The effect of different surgical drilling procedures on full laseretched microgrooves surfacetreated implants: an experimental study in sheep. Clin Oral Implants Res 2014; 25(9):1072-1077.
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Anchieta RB, Baldassarri M, Guastaldi F, Tovar N, Janal MN, Gottlow J, Dard M, Jimbo R, Coelho PG. Mechanical Property Assessment of Bone Healing around a TitaniumZirconium Alloy Dental Implant. Clin Implant Dent Relat Res 2014; 16(6):913-919.
Hayashi M, Jimbo R, Xue Y, Mustafa K, Andersson M, Wennerberg A. Photocatalytically induced hydrophilicity influences bone remodelling at longer healing periods: a rabbit study. Clin Oral Implants Res 2014; 25(6):749-754.
Bougas K, Jimbo R, Vandeweghe S, Tovar N, Baldassarri M, Alenezi A, Janal M, Coelho PG, Wennerberg A. In vivo evaluation of a novel implant coating agent: laminin1. Clin Implant Dent Relat Res 2014; 16(5):728-735.
Halldin A, Jimbo R, Johansson CB, Wennerberg A, Jacobsson M, Albrektsson T, Hansson S. Implant stability and bone remodeling after 3 and 13 days of implantation with an initial static strain. Clin Implant Dent Relat Res 2014; 16(3):383-393.
Larsson C, Wennerberg A. The clinical success of zirconiabased crowns: a systematic review. Int J Prosthodont 2014; 27(1):33-43.
Melin Svanborg L, Meirelles L, Franke Stenport V, Kjellin P, Currie F, Andersson M, Wennerberg A. Evaluation of bone healing on sandblasted and acid etched implants coated with nanocrystalline hydroxyapatite – An in vivo study in rabbit femur. Int J Dent. 2014; e197581.
Chrcanovic B, Albrektsson T, Wennerberg A. Reasons for failures of oral implants. J Oral Rehabil. 2014 Jun; 41(6):443-476.
Chrcanovic B, Albrektsson T, Wennerberg A. Flapless versus conventional flapped dental implant surgery: a metaanalysis. PLos One 2014; 20:9(6):e100624.
Chrcanovic B, Albrektsson T, Wennerberg A. Immediate nonfunctional versus immediate functional loading and dental implant failure rates: A systematic review and metaanalysis. J Dent 2014;42(9):1052-1059.
Chrcanovic B, Albrektsson T, Wennerberg A. Prophylactic antibiotic regimen and dental implant failure: a metaanalysis. J Oral Rehabil 2014;41(12):941-956.
Braian M, De Bruyn H, Fransson H, Christersson C, Wennerberg A. Tolerance measurements on internal and externalhexagon implants. Int J Oral Maxillofac Implants 2014;29(4):846-852.
Albrektsson T, Dahlin C, Jemt T, Sennerby L, Turri A, Wennerberg A. Is marginal bone loss around oral implants the result of a provoked foreign body reaction? Clin Implant Dent Relat Res 2014;16(2):155-165.
Chrcanovic BR, Albrektsson T, Wennerberg A. Tilted versus axially placed dental implants: A metaanalysis. J Dent 2014; S0300-5712(14)00261-9.
Chrcanovic BR, Albrektsson T, Wennerberg A. Immediately loaded nonsubmerged versus delayed loaded submerged dental implants: A metaanalysis. Int J Oral Maxillofac Surg. 2014; doi: 10.1016/j.ijom.2014.11.011.
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CONTRIBUTIONS TO AND pARTICIpATION IN CONFERENCES AND wORkSHOpS
oRAl pResentAtions 2014Ann Wennerberg. “Implantates: Superfieces modernas e pesquisas atuais”, 32 CIOSP Congresso Internacional São Paulo, 31 Jan 2014, Brazil. Invited lecture
Ann Wennerberg. “Why does implant fail? Thinking outside the box”, 3 March 2014, George, South Africa. Invited lecture
Ann Wennerberg. Career achievments, WISE Women in Science, 4 Sep 2014, Chalmers University of Technology, Gothenburg, Sweden. Invited lecture
Ann Wennerberg. “How research of today can improve products and treatment results in the future”, Tylösandsdagarna 2014, Joint meeting SFOP and SSPD, 10–12 Sep 2014, Tylösand, Sweden. Invited lecture
Ann Wennerberg. “Dental implants at a mm, mico and nanometer aspect”, DSOI annual meeting, 24 Oct 2014, Vejle, Denmark. Invited lecture
Anette Gjörloff Wingren. “Cells and biomarkers in cancer from conventional methods to innovative digital holographic imaging”. Malmö Cancer Center Retreat 2014, 28–29 August 2014, Båstad, Sweden. Invited lecture
Börje Sellergren. “Molecular Imprinting and adaptable monolayers: Versatile concepts in supramolecular chemistry”, GDCh Minisymposium Supramolecular Polymers, Dec 2014, Essen, Germany. Invited lecture
Börje Sellergren. “Smart capture phases for bioanalysis and discovery“, International Conference on Molecular imprinting, 18–21 Sep 2014, Zhenjiang, China. Invited lecture
Börje Sellergren. “Diagnostic tools based on plastic antibodies”, Innovativ diagnostik för framtiden, Skåne Innovation Week, Medeon, Malmö, June 2014. Invited lecture
Börje Sellergren. “Molecularly imprinted polymers for environmental technologies”, Swiss Federal Institute of Aquatic Science and Technologies, Dec 2014, Zürich, Switzerland. Invited lecture
Börje Sellergren. “Where antibodies fail: Smart capture phases for bioanalysis and discovery”, National University of Singapore, 8 May, 2014, Singapore. Invited lecture
Gunnel Svensäter. “Discovery of new biomarkers and effective interventions”, 23 Apr 2014, Brånemarkkliniken, Göteborg, Sweden. Invited lecture
Gunnel Svensäter. ”Infection control on the biofilm level”, Läkarutbildningen, 10 Nov 2014, Örebro University, Sweden. Invited lecture
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Gunnel Svensäter. “Oral Biofilms and Strategies for Infection Control”, European Society of Endodontology, 17 Oct 2014 Amsterdam, The Netherlands. Invited lecture
Gunnel Svensäter. ”Bakterier på gott och ont”, Akademisk kvart på Stadsbibliotek, 2014, Malmö, Sweden. Invited lecture
Johan Engblom. “A water gradient can be used to regulate biophysical properties of skin and mucosa”, 2nd Pharm. Tech IAPST International Conference on “New insights into diseases and recent therapeutic approaches”, 17–19 Jan 2014, Kolkata, India. Invited lecture
Johan Engblom. “A Water Gradient can be used to Regulate Biophysical Properties of Skin and Mucosa and Impact Drug Transport Monash University”, 24 Jan 2014, Melbourne, Australia. Invited lecture
Julia Davies. ”Oral biofilms”, 14 Feb 2014, Department of Infection Medicine, Lund University, Sweden. Invited lecture
Marité Cárdenas Gómez. “Biomimetic surfaces for membrane bound protein structural and functional studies”, 13th Surface Xray and Neutron Scattering International Conference, 7–11 July 2014. Hamburg, Germany. Invited lecture
Marité Cárdenas Gómez. “Film composition and its relevance in pharmacological formulations”, Open Seminar ESS and MAX IV – new opportunities in formulation research, 9 Jan 2014, Lund, Sweden. Invited lecture
Sergey Shleev. “Hybrid electric power devices for simultaneous generation and storage of electric power” Nanotechnology Conference organized by Lithuanian Academy of Sciences, 16 May 2014, Vilnius, Lithuania. Invited lecture
Tautgirdas Ruzgas. “Nanoelectrochemical sensors, theranostics, and noninvasive sensing”, IVA’s Division for Basic and Interdisciplinary Engineering Sciences, 22 Sep 2014 Stockholm, Sweden. Invited lecture
Vitaly Kocherbitov. “Thermodynamics of biopolymer water interactions”, Technical Chemistry: from Theory to Practice. October 2014, Perm, Russia. Invited plenary
Vitaly Kocherbitov. “Application of scanning methods to distinguish between entropy and enthalpy driven phase transitions”, Interactions in Colloidal Systems: COST action workshop, March 2014, Berlin, Germany. Invited lecture
Dmitry Pankratov. “Hybrid electric devices for simultaneous generation and storage of electric power”, ESEAC2014, 11–15 June 2014, Malmö, Sweden.
Hala Gari. “Amperometric monitoring of drug penetration through skin”, ESEAC2014, 11–15 June 2014, Malmö, Sweden.
Javier Sotres. “Salivary Films – What can we learn from nanomechanical studies?”, 10th European Symposium on Saliva, May 2014, Egmond aan Zee, The Netherlands.
Liselott Lindh. “Influence of substratum hydrophobicity on salivary film composition” 10th European Symposium on Saliva, May 2014, Egmond aan Zee, The Netherlands.
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Biofilms – Research Center for Biointerfaces
Annual Report 2014
Patrick Seumo. “Electrochemical determination of total protein concentration”, ESEAC2014, 11–15 June 2014, Malmö, Sweden.
Vida Krikstolaityte. “Mediatorless carbohydrate/oxygen biofuel cells with improved cellobiose dehydrogenase based bioanode”, ESEAC2014, 11-15 June 2014, Malmö, Sweden.
Vitaly Kocherbitov. “Application of sorption calorimetry for studies of hydration of biomolecules”, XVIII conference of International Society for Biological Calorimetry, 1–4 June 2014, Lund, Sweden.
Vitaly Kocherbitov, “Application of humidity scanning QCMD for characterization of sorptiondesorption hysteresis in biopolymers”, 28th ECIS Conference, 7-12 September 2014, Cyprus.
Vitaly Kocherbitov. “Molecular dynamics simulations of nonlamellar liquid crystalline phases of surfactants”, 20th Surfactants in Solution conference, 22–27 June 2014, Coimbra, Portugal.
contAct infoRmAtionbiofilms – research center for biointerfacesMalmö universityFaculty of Health and Society205 06 MalmöSweden
center director : Doctor Anna Holmbergtel : +46 40 665 79 35 e -mail : [email protected]/brcb