Complex Genetics Made SimpleTM

InitialDetection

Progressionand Pathology

HereditaryPredisposition

Minimal Residual Disease Monitoring

RainDance Technologies is leading a transformation in disease research by making ultra-sensitive picodroplet-based solutions that simplify genetic analysis. Our Next-Generation Sequencing (NGS) and Digital PCR (dPCR) products are fundamentally improving the way scientists examine cell-based and cell-free biomarkers for hereditary risk predis-position, initial detection, pathology, and minimal residual disease in cancer and other complex inherited disorders or infectious diseases.

RainDance offers a complete portfolio of products based on its core RainStorm™ digital droplet tech-nology that enables novel, non-invasive liquid biopsy research applications for more accurate, reliable, cost-effective, and early detection of cancer and other complex inherited disorders or infectious diseas-es. Major research institutions and laboratories around the world rely on the proven performance of the following products:

• RainDrop® Digital PCR System: Ultra-sensitive dPCR system used in research for the early detec-tionand monitoring of cell-free and cell-based biomarkers for cancers, viruses, pathogens, and immune disorders.

• ThunderBolts™ NGS Enrichment System: ‘Democratized’ NGS system enabling highly sensitive, rapid, low input, tumor profiling research in liquid biopsy, tissue and FFPE samples.

• ThunderStorm® NGS Enrichment System: Industrial-scale workhorse for highly accurate, fully auto-mated targeted NGS enrichment.

Comprehensive Next-Gen SequencingPanel for Profiling Important

Cancer Mutations

Myeloid Panel

Comprehensive Next-Gen SequencingPanel for Profiling Important

Cancer Mutations

Next-Gen Sequencing Enrichment

Ultra-Sensitive Digital PCR Systemfor Detecting Mutations Directly

From a Fluid Biopsy™

Ultra-Sensitive Digital PCR

Fully Automated Next-Gen SequencingContent Enrichment System

for High-Throughput Next-Gen Sequencing

Enrichment

Comprehensive Next-Gen SequencingPanel for Profiling Important

Cancer Mutations

Cancer Panel

Comprehensive Next-Gen SequencingPanel for Profiling Important

Cancer Mutations

Open Source

RainDrop® Digital PCR SystemEnable new ultra-sensitive, non-invasive liquid biopsy mutation detection applications

The RainDrop Digital PCR System is the industry’s most sensi-tive nucleic acid detection and monitoring analysis platform. The digital answers fundamentally change the performance of molecular assays for important research applications including low-frequency tumor allele detection, genotyping, gene expres-sion, copy number variation, viral quantitation, and direct RNA detection. Initial

DetectionMinimal Residual Dis-

ease Monitoring

Prepare Sample and Assay

Target

Target

Background

Source

Partition in Droplets Amplify Analyze

Sense

Count

PCR+ ”bright” droplets

PCR- ”dark” droplets

Streamlined Workflow

Advantages

• Sensitivity: Provide lower limit of detection in range between 1 in 20,000 to 1 in > 1,000,000

• Absolute quantitation: Quantify specific targets without reference to a standard curve

• Dynamic range and precision: Generate up to 10 million picoliter-sized droplets/lane enabling true single molecule analysis without pre-measurement

• Multiplexing: Conduct multiplexed tests on the same sample using the single molecule multi-color and varying probe intensity approach

• Liquid biopsy applications: Interrogate cell-free circulating DNA that is often highly fragmented and only available in low quantities

• Single pipetting step and closed-tube design: Ensure highest quality by minimizing contamination or carryover

• Versatile open source platform: Utilize standard qPCR reagents, master mixes, and also run ThunderBolts NGS Enrichment Panels

• Proven technology: Leverage a decade of experience with the same core technology found in RainDance’s NGS Content Enrichment Systems

RainDance Products are for Research Use Only. Not for use in diagnostic procedures.

ThunderBolts™ NGS Enrichment System Rapidly detect and cost-effectively analyze somatic mutations as low as 1% in precious amounts of liquid biopsy or FFPE

The ThunderBolts System is a fast, accurate and low-cost NGS target enrichment system that features award-winning instru-mentation and pre-validated gene panels, as well as flexible open source options for scientists looking for custom solutions. The fast and simple workflow is optimized for Illumina NGS systems and compatible with liquid biopsy, FFPE and tissue samples with as low as 10 ng of starting DNA. Researchers gain rapid, accurate mutation profiles for low overall cost per sample.

Progressionand Pathology

Prepare Samples,Primers and PCR Reagents Enrich Gene Targets

Analyze withIllumina NGS

KRASEGFR

TP53

KRAS

TP53

EGFR

Background

Streamlined Workflow

Advantages

• Single molecule droplet PCR: Generate 8 million PCR picodroplet reactions per well

• Pre-validated gene panels: Run the following panels that were developed in collaboration with a consortium of industry experts:

ThunderBolts™ Cancer Panel: Interrogate mutations/hotspots in 50 oncogenes, tumor suppressors and drug resistance markers

ThunderBolts™ Myeloid Panel: Profile mutations in 49 myeloid genes for research related to AML, MDS, MPN and other myeloid disorders.

• Minimal and flexible sample input requirements: Analyze a range of sample types (plasma, FFPE and tissue) with as low as 10 ng amplifiable DNA

• Streamlined workflow for Illumina NGS: Eliminate sequencing library preparation steps with RainDance DirectSeq™ workflow

• Highly sensitive: Detect minor allele frequencies as low as 1%

• Reduced time to result: Process 8 samples with < 3 hours hands-on time, enabling sample-to-results in < 2 days

• Custom content options: Create custom gene content panels using the ThunderBolts Open Source Consumables Pack

• Low cost per sample: Highly cost-effective sample-to-sequencing data economics

RainDance Products are for Research Use Only. Not for use in diagnostic procedures.

ThunderStorm® NGS Enrichment SystemApply gold-standard PCR enrichment more cost-effectively, and at higher throughput using any NGS platform

The ThunderStorm System is a fully automated, high-throughput and high performing (coverage, sensitivity and completeness) NGS target enrichment solution that enables researchers to process more samples per day, and generate higher-quality data faster than ever before. Researchers can apply NGS technology more routinely in applications such as rare variant detection, inherited disease research (carrier typing, constitutional disorders, etc.), methylation, and deep sequencing of somatic tumor samples.

HereditaryPredisposition

Select Genomic ROI / Gene Targets

Enrich Gene Targets Analyze with NGSCreate Droplet-basedGene Panel

Streamlined Workflow

Advantages

• Fully automated high-throughput system: Process up to 96 samples with as little as 15 minutes hands-on time and generate up to 48 sequence-ready samples in 24 hours

• Sophisticated primer design: Target up to 20,000 genomic loci in a single sample with > 99% coverage

• Single molecule droplet PCR: Maximize sequencer efficiency and minimize costs with greater coverage uniformity and completeness

• Coverage of challenging regions: Study all genetic variations contained in any desired region of the genome, including repetitive, high G-C rich and pseudo gene regions

• Application specific primer panels: Design and create custom content target gene panel for genetic disease (germline or somatic) research

• Flexible format: Access up to 8 different gene libraries (primer panels) in a single run

• RainDance DirectSeq™ workflow: Eliminate sequencing library preparation steps

• Compatible with all NGS platforms: Utilize any of the available leading NGS platforms for analysis

RainDance Products are for Research Use Only. Not for use in diagnostic procedures.

About RainDance Technologies

RainDance Technologies is making complex genetics simple. The company’s ultra-sensitive genomic tools enable better research of novel non-invasive liquid biopsy applications that should result in more accurate, reliable, cost-ef-fective, and early detection of cancer, inherited and infectious diseases. Major research institutions and laboratories around the world rely on the performance of RainDance systems. Based outside of Boston, Massachusetts, the company supports customers using RainDrop® Digital PCR, as well as the ThunderStorm® and ThunderBolts™ Next-Generation Sequencing Enrichment Systems through its international sales and service operations as well as a global network of distributors and commercial service providers. Both technology platforms have been recog-nized by The Scientist as Top 10 Innovations with the RainDrop System winning the award in 2014 and Thunder-Storm System in 2011. For more information, please visit www.RainDanceTech.com.

RainDance Technologies749 Middlesex TurnpikeBillerica, MA 01821

Phone: 978-495-3300Fax: 978-528-6090info@raindancetech.com

The RainDance products are for Research Use Only. Not for use in diagnostic procedures.

RainDance Technologies, the RainDance Technologies logo, RainDrop, ThunderStorm, ThunderBolts, and RainStorm are trade-marks of RainDance Technologies, Inc. All other brands may be trademarks of their respective holders.

©2015 RainDance Technologies, Inc. All rights reserved. Printed in USA

LCN: 50-04381 Rev D