biological safety cabinets

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Biological SafetyBiological Safety

CabinetsCabinets

Marcy HueyMarcy Huey

August 2009August 2009



Hazard Levels?Hazard Levels?

What are you talking about??!!What are you talking about??!!

Hazard GroupHazard Group 1 2 3 4 (of pathogens)1 2 3 4 (of pathogens) Containment LevelContainment Level 1 2 3 4 (of laboratories)1 2 3 4 (of laboratories)

ClassClass 1 2 3 4 (of Genetic Modification)1 2 3 4 (of Genetic Modification)

CLASSCLASSI II III (of Safety Cabinets)I II III (of Safety Cabinets)



The terminologyThe terminology ± ±µSafety¶CabinetsµSafety¶Cabinets

This is one of the most common mistakes madeThis is one of the most common mistakes made

Tissue Culture Hood, Laminar Flow, Glove Box, FumeTissue Culture Hood, Laminar Flow, Glove Box, Fume

Hood,Hoods, Cabinets, IsolatorHood,Hoods, Cabinets, Isolator ± ± not all exactly thenot all exactly the

samesame

Microbiological Safety Cabinet Class I, II or IIIMicrobiological Safety Cabinet Class I, II or III

Also calledHorizontal Laminar Flow/ VerticalAlso calledHorizontal Laminar Flow/ Vertical

Laminar FlowLaminar Flow

Sometimes called IsolatorsSometimes called Isolators

Or Fume CupboardsOr Fume Cupboards



µContainment Level¶of LaboratoriesµContainment Level¶of Laboratories

CL1:CL1:--³I¶ll just do it on the bench over ³I¶ll just do it on the bench over

there«..´there«..´

CL2:CL2:--³OK, so I¶ll have to use the hood««.´³OK, so I¶ll have to use the hood««.´ CL3:CL3:--³That¶s that room along the corridor that³That¶s that room along the corridor that

nono--one goes in?´one goes in?´

CL4:CL4:--³Spacesuits?´³Spacesuits?´




CL1:CL1:--easy to clean, impervious resistant surfaces, easy to clean, impervious resistant surfaces, disinfectants to hand,minimise aerosols, door shutdisinfectants to hand,minimise aerosols, door shutduring work. GLPduring work. GLP --lab coats and PPE, no mouthlab coats and PPE, no mouth

pipetting, eating etc etc, hand wash sink , safe disposal pipetting, eating etc etc, hand wash sink , safe disposalroutes& in addition to the above«.routes& in addition to the above«.

CL2:CL2:--authorised access only, specified disinfectionauthorised access only, specified disinfection procedures, safe storage of biological agents, work procedures, safe storage of biological agents, work

producing aerosols must be in MSC,access to producing aerosols must be in MSC

,access toincinerator for some bio waste, 24m3 space per incinerator for some bio waste, 24m3 space per

person, autoclave, negative airflow during work if person, autoclave, negative airflow during work if possible, usually require BSC possible, usually require BSC




CL3:CL3:--Must be maintained at negative pressure, Must be maintained at negative pressure, sealable for fumigation,& extracted air must besealable for fumigation,& extracted air must beHEPA filtered.Must be separated from any other HEPA filtered.Must be separated from any other

activities in the building.Secure access & list of activities in the building.Secure access & list of authorised personnel posted on entrance. O bservationauthorised personnel posted on entrance. O bservationwindow.Laboratory to contain its own equipment, window.Laboratory to contain its own equipment, BSC & autoclave.Impervious floor as well asBSC & autoclave.Impervious floor as well as benches.Written contingency plans in case of benches.Written contingency plans in case of spillage. Written training records.& in addition to thespillage. Written training records.& in addition to theabove«.UA doesn¶t currently have any CL3 labs.above«.UA doesn¶t currently have any CL3 labs.

CL4:CL4:--The University has no CL4 labs.The University has no CL4 labs.



BSC vs Fume HoodBSC vs Fume Hood

Biological Safety Cabinets (BSC) provide:Biological Safety Cabinets (BSC) provide:

Protection for the user and the productProtection for the user and the product

HEPA filtration of air into cabinet and exhaust air HEPA filtration of air into cabinet and exhaust air Fume hoods provide:Fume hoods provide:

R emoval of contaminated air R emoval of contaminated air

Protection for the user from chemicalsProtection for the user from chemicals



BSCs vs Fume HoodsBSCs vs Fume Hoods

Biological Safety Cabinets provide user Biological Safety Cabinets provide user

and product protection via HEPAand product protection via HEPA

filtered air circulation.filtered air circulation. Fume Hoods provide user protectionFume Hoods provide user protection

via removal of contaminated air to externalvia removal of contaminated air to external

exhaust.exhaust.



Biological Safety CabinetsBiological Safety Cabinets

Must be properly maintained and regularlyMust be properly maintained and regularly

servicedserviced

Must be certified annually and when new, Must be certified annually and when new, moved, or repairedmoved, or repaired

Must be used in conjunction with goodMust be used in conjunction with good

microbiological techniquesmicrobiological techniques

Provide protection for personnel, product, andProvide protection for personnel, product, and

environmentenvironment



General Cabinet InformationGeneral Cabinet Information

Types of Biological Safety CabinetsTypes of Biological Safety Cabinets

Choose the correct Biological Safety Cabinet for your Choose the correct Biological Safety Cabinet for your experiments.experiments.

Contact the Department of Environmental Health andContact the Department of Environmental Health andSafety before purchasing a new cabinet or beginningSafety before purchasing a new cabinet or beginninga new project for help in selecting the appropriatea new project for help in selecting the appropriatecabinet.cabinet.

Biosafety in Microbiological and BiomedicalBiosafety in Microbiological and Biomedical

LaboratoriesLaboratorieshttp://www.cdc.gov/od/ohs/ biosfty/ bmbl5/ bmbl5toc.hhttp://www.cdc.gov/od/ohs/ biosfty/ bmbl5/ bmbl5toc.htmtm

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