biologically based fertilizer recommendations to meet yield expectations and preserve water quality

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Franzluebbers and Stuedemann (2010) Soil Sci. Soc. Am. J. 74:2131-2141

Soil Organic Carbon Sequestration (kg . ha

-1 . yr

-1)

0 200 400 600 800 1000

TotalSoil

NitrogenAccumulation

(kg . ha

-1 . yr

-1)

0

20

40

60

80

100TSN = 5.7 + 0.102 (SOC)

r2 = 0.98

Relationship between soil C and N accumulation

Linkage of nitrogen with active carbon

Soil microbial activity biologically sequesters N into

organic matter

Soil process relationships

Franzluebbers et al. (1999) Soil Sci. Soc. Am. J. 64:613-623

Available Nitrogen (kg ha-1

)

RelativeYield

(fraction)

0.0

0.2

0.4

0.6

0.8

1.0Sites with high N

availability and low N fertilizer response

Goal of enlarging the biologically active N pool without causing

N leakage

Inorganic nitrogen Surface soil Residual in profile

Organic nitrogen Long-term stable Biologically active

Accounting for

Available Nitrogen (kg N ha-1)

Sites with low N availability and high N fertilizer response

Idealized response to nitrogen

Virginia Tech – essential findings

Hairy vetch cover crop increased corn yield

• more than the N credit it supplied

Soil nitrate at V4 was correlated with N uptake of the previous cover crop

Mineralizable C (1 and 28 day tests) prior to planting and at V4 was not predictive of unfertilized corn yield at 4 sites

Soil nitrate at V4 was correlated with unfertilized corn yield (r = 0.45)

Predicting N supply from cover crops and soil organic matter with ecologically-based models

318.03.8 ,10

):(40.01max0084.0 NO

NCNN

Y

whccwhccwhwkcc

where,

ngmineralizi N and tillage-no if0.020,

ngmineralizi N and tillageif0.034,

ngimmobilizi N and tillageif0.12,

wh

Step 1: Cover Crop N Supply Model

Difference in unfertilized corn yield (Mg/ha) between a cover cropped soil and a bare fallow reference

Efficiency of N supply is controlled by tillage and N mineralization

vs. immobilization

Pre-emptive competition for

soil NO3- reduces

N supply

Winterkilled and winterhardy species have different N

supply regulators

N mineralization/ immobilization is controlled by microbial carbon use

efficiency and biomass stoichiometry

White et al. 2016. A model data-fusion approach for predicting cover crop nitrogen supply to corn. Agronomy Journal. doi:10.2134/agronj2016.05.0288

Predicting N supply from cover crops and soil organic matter with ecologically-based models

Best Model (r2=0.67) Unfertilized Corn Yield (Mg/ha) = -3.6 + 3.2*Soil %C + 0.14*% sand + 0.054*% silt + 0.46*Cov.Crop N Credit

Model with CO2 Burst (r2=0.60) Unfertilized Corn Yield (Mg/ha) = 2.9 + (0.00017 * CO2Burst2)† + 0.14*% sand + 0.48*Cov.Crop N Credit

†Term is only included in tilled soils

Step 2: Soil Organic Matter N Supply Model • Calibrated with unfertilized corn yields from 5 cover crop experiments (119

plots) • N supply from cover crop residues was isolated using the previously

calibrated cover crop N supply model • Find the best predictors of N supply from soil organic matter

• Tested total soil %C, 24hr CO2 burst, soil particle size fractions, tillage vs. no-till in a general linear model stepwise selection process

NC State approach

Plant N uptake in semi-controlled greenhouse experiments being explored

Plant N uptake in minor relationship with total organic C

Pershing (2016) NC State University MS thesis

Plant N uptake in strong relationship with mineralizable N

Pershing (2016) NC State University MS thesis

Plant N uptake in reasonable relationship with the flush of CO2

Pershing (2016) NC State University MS thesis

Field trials to evaluate biological N supply

Example of 3 strips fertilized with 0, 69, and 125 kg N ha-1 at sidedress

- Corn grain and silage in North Carolina and Virginia

1 2 3 4

Soil sampling (8 cores from each of 4 replicate locations)

Soil analyses Flush of CO2, net nitrogen mineralization Routine soil testing for pH, P, K, other elements (NC Dept Agric) Bulk density, particle size, total C-N, microbial biomass C, inorganic N

20 gal/acre sidedress

36 gal/acre sidedress

No sidedress

Nitrogen treatments applied 26 June 2015 (32 rows each)

Yield harvest (18’ row sections at 12 points in each strip)

Plant analyses Dry matter yield, stand density, nutrient concentration of forage, including protein, fiber, minerals (Ca, P, S, Mg, Na, K, Cu, Fe, Mn, Zn), ADF, NDF, nitrate (NC Dept Agric)

Collected yield estimates on 21 August 2015

Yield Results

Sidedress N Application (lb N/acre)

0 40 80 120

CornSilageYield

(ton/acre)[35% DM]

0

10

20

30

40

Rep 1

Yield Results

Sidedress N Application (lb N/acre)

0 40 80 120

CornSilageYield

(ton/acre)[35% DM]

0

10

20

30

40

Rep 2

Yield Results

Sidedress N Application (lb N/acre)

0 40 80 120

CornSilageYield

(ton/acre)[35% DM]

0

10

20

30

40

Rep 3

Yield Results

Sidedress N Application (lb N/acre)

0 40 80 120

CornSilageYield

(ton/acre)[35% DM]

0

10

20

30

40

Rep 4

Yield Results

Sidedress N Application (lb N/acre)

0 40 80 120

CornSilageYield

(ton/acre)[35% DM]

0

10

20

30

40

Overall (n = 12)

Average yield = 23.6 ton/acre

Implications

Sidedress N Application (lb N/acre)

0 40 80 120

CornSilageYield

(ton/acre)[35% DM]

0

10

20

30

40

Overall (n = 12)

Wheat grain evaluations in North Carolina

Flush of CO2 following Rewetting of Dried Soil

(mg CO2-C kg

-1 soil)

0-3 days

0 100 200 300 400 5000.0

0.2

0.4

0.6

0.8

1.0

1.2

Relative

Yield

Without

In-Season

Nitrogen

Wheat

Tall fescue

Corn

Preliminary results

Unpublished data

We’re getting excited about the potential!