Biology: A Molecular Approach, Laboratory

Welcome!

Today’s Plan

• Orientation• Laboratory Notebooks• The Scientific Method• Controlled Experiments

ORIENTATION

Introductions - Instructors• Dr. Jackie Crisman

• Lecture

• Dr. Ellen Lehning

• Lab• Dr. Susan Greenwood

• Laboratory Technician

Introductions - Students• Name• Why you are in this class• Any thing else you’d like to tell us

Introductions - Biotechnology• Use a marker to put your name on a

name tag• Count-off from 1 to 7

• All 1s, 2s, 3s, etc. get into a group• As a group, answer the following

question:• What is biotechnology?

• Pick someone to be your spokesperson

• I’ll call on each group’s spokesperson to share their answer

Our Focuses• Research, research, research!

• Technique, technique, technique!

• Control, control, control!

• Professional but fun!

Syllabus and Tentative Schedule

Laboratory Safety Contract• Read the handout on Laboratory Safety

• Tear-off the bottom strip, sign, and turn-in the Laboratory Safety Contract

• Note the location of the following safety equipment• Glass waste, Fire extinguishers,

Eyewashes, First aid kit, Exits, Phone

Laboratory Supplies• We are supplying you with:

• Lab coats• Lab safety glasses• Gloves• Lab notebooks• All handouts

• You need to bring a calculator

Professional Conduct

• Read through the Professional Conduct Rubric

LABORATORY NOTEBOOK

Laboratory Notebook Preparation• You will work in your groups for the

rest of the semester• You will fillout your laboratory

notebook together as a group• Read the handout on Laboratory

Notebook Preparation• Read the Lab Notebook Rubric• Get the front of your notebook, title

page, and table of contents setup• Use the PCR example provided as a

reference for the rest of the semester

THE SCIENTIFIC METHOD

The Scientific Method

FundingCommunicate

CONTROLLED EXPERIMENTS

Controlled Experiments

All samples handled as nearly as humanly possible exactly the same way

Use positive controls to eliminate false negatives Use negative controls to eliminate false positives Ensures results are true!

Controlled Experiments

Read the “Bad Boys” handout Answer the following questions?

What DNA samples were collected and analyzed?

Why were they collected and analyzed? I am randomly going to call on one group and one

member of that group to answer these questions

Controlled Experiments

Get out the Using Controls in an Experiment handout

Work through problems 3 – 7 I am randomly going to call on one group and one

member of that group to answer these questions

To Print

• Syllabus• Tentative Schedule• Laboratory Safety

Contract• Professional Conduct

Rubric• Lab Notebook

Preparation• Lab Notebook Rubric• PCR notebook example• How to Design a Properly

Controlled Experiment• Practice with Controls

To Collect

• Name tags/Markers• Lab books• Chime and selector