biomass processing
TRANSCRIPT
BIOETHANOL LABORATORY
IQ/COPPE - UFRJ
R&D Activities on Biomass Processing
ELBA P. S. BON
A inauguração do Laboratório de Bioetanol (29/08/2013)
11/05/2011
FINANCIAL SUPPORT
I – BIOETANOL II Project
“Scaling up of cellulases and accessory enzymes production
and use for the hydrolysis of pretreated biomass”
Ref. FINEP 1421-08
2009 – 2016
R$ 9,9 milhões
II –JICA/JST PROJECT
“Research on sustainable biofuel production from
lignocellulosic biomass resources”
2009 – 2013
$ 2.4 milhões
JICA supported the Project activities in Brazil and JST
supported the activities developed in Japan at the Biomass
Refinery Research Center - National Institute of Advanced
Industrial Science and Technology (AIST).
III –COLABORATION PROJECT WITH THE EUROPEAN UNION
“Integration of biology and engineering into an economical and energy-efficient 2g bioethanol biorefinery”
2011 – 2015
R$ 3.122.457,00
IV –MICROALGAE PROJECT
“Microalgae production, characterization and fractionation for
the production of biofuels and bioactive molecules”
2013 – 2016
R$ 600.000,00
V – CONTRACTS WITH COMPANIES
BIOMASS COMPOSITION
BIOMASS PROCESSING
ENZYMES
CONSULTANCY
NON ACIDICS BIOMASS PRETREATMENTS
BIOMASS REFINERY RESEARCH CENTER / AIST (JAPAN)
BIOMASS ENZYMATIC HYDROLYSIS
GLUCOSE SYRUPS
60 g/L
(87% HYDROLYSIS
YIELDS)
FERMENTATION
LIGNIN
(solid fuel)
Project results Ethanol 2G perspectives
Biomass availability
Biomass Pretreatment
Enzyme Production
Enzyme characterization
Enzymatic Hydrolysis
LCA analysis
Ethanol 2G perspectives
Biomass availability
Disponibilidade de biomassa: 12% bagaço (18 Kg) + 50% palha (153 Kg)
36,5L de etanol originado de celulose
(assumindo 30% de celulose e 0.79 g/cm3 de densidade de etanol)
1 ton of CANA
Enzyme Production
Brazilian patent on enzyme compositium
PATENT - N.PCT/BR2008/00034 19/01/2009
ENZYME COMPOSITION AND USES THEREOF, PRODUCTION PROCESS FOR ENZYMES
AND PRODUCTION PROCESS OF ALCOHOL USING THE ENZYME COMPOSITION.
Aspergillus spTrichoderma sp
ENZYME BLEND
High β-glucosidase High xylanase Low FPase
High FPase High xylanase Low β-glucosidase
Enzyme characterization
&
Enzyme activity assays
Biomass Pretreatment
Steam explosion alters the natural composition of sugarcane bagasse by removing most of its hemicellulose part
Ball milling reduces the cristalinity and the particle size of sugarcane biomass
Wet disk milling causes defibrillation of the sugarcane biomass
Bagasse: [Emim] [Ac] mass ratio 1:20
20 nm nanofibers!
(a) Raw bagasse; (b) O3 treated bagasse after 60 min. (c) 7 cycles WDM treated bagasse;(d)WDM + O3 treated bagasse; (e) Raw straw; (f) O3 treated straw after 60 min. (g) 7 cyclesWDM treated straw; (h) WDM + O3 treated straw;
Enzymatic Hydrolysis
Fermentation
Life cycle assessment - LCA
UFRJ (Brazil) – Frunhofer (German) collaboration
1G+2G
Microalgae as source of fermetable sugars
Cellulose nanocrystals
Disk milled After hydrolysis with EGPho
Biohydrogen production
Equipe
www.bioetanol-ufrj.com.br
SUPORTE FINANCEIRO
Agradeço
UFSC