bioo scientific - improving ngs library prep automation on the sciclone ngs workstation
TRANSCRIPT
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IMPROVING NGS LIBRARYPREP AUTOMATION
on the
SCICLONE NGS WORKSTATION
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Typical NGS workflows offer challenges with respect to throughput,
cost & uniformity
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Use of automated liquid handling workstations can provide a cost-
effective solution to many ofthese workflow challenges
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Rapid & robust workflows which are easy to deploy are critical for success
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How is Bioo Scientific improving library prep automation on the Sciclone?
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EASY DEPLOYMENT
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Bioo Scientific has developed & validated a number of automated RNA-Seq &
DNA-Seq Illumina-compatible library prep programs for use on the Sciclone
NGS and NGSx Workstations
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RAPID & ROBUST WORKFLOWS
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The NEXTflex RNA-Seq and DNA-Seq Kits automated on the Sciclone all incorporate
fast, user-friendly protocols
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INCREASED MULTIPLEXING CAPABILITIES
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Up to 384 unique, single index barcodesare available for deep multiplexing of
RNA-Seq & DNA-Seq libraries
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REDUCED RNA-SEQ BIAS
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Bias-reducing Molecular Indexes allow for more accurate resolution of duplicates
introduced during PCR amplification step of stranded RNA-Seq library construction on
the Sciclone NGS or NGSx
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Absolute Quantitationfor RNA-Seq
Molecular Indexes are included in the NEXTflex™ Rapid Directional qRNA-Seq™ Kit
®
DOWNLOAD MANUAL VIEW SLIDESHARE
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IMPROVED LIGATION EFFICIENCY
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The NEXTflex DNA-Seq & RNA-Seq library prep kits automated on the Sciclone all incorporate Bioo Scientific’s Enhanced Adapter Ligation Efficiency Technology
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Which increases library diversity & complexity, thus allowing for use of
degraded or smaller amounts of input DNA
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OPTIMIZED FOR AUTOMATION
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The NEXTflex DNA-Seq & RNA-Seq library prep kits include master-mixes
optimized for automation
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Download the Sciclone NGS and NGSx Workstation Automation Guides:
®®®®
NEXTflex™ Rapid DirectionalqRNA-Seq™ Kit Automation Guide
NEXTflex™ Rapid DirectionalRNA-Seq Kit Automation Guide
NEXTflex™ Rapid RNA-SeqKit Automation Guide
NEXTflex™ Rapid DNA-SeqKit Automation Guide
DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL DOWNLOAD MANUAL
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Or contact one of our Automation Specialists today to improve your automated NGS library
prep using the Sciclone NGS Workstation
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If you have any questions email us [email protected]
or visit our website atBiooScientific.com/Sciclone