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Bioremediation Pilot StudyPassaic River/Newark Bay
John Pardue, Ph.D. - Louisiana State University� Elizabeth Howell Stewart Professor of Civil and Environmental
Engineering at Louisiana State University
Donna Fennell, Ph.D. - Rutgers University� Associate Professor, Department of Environmental Sciences
and Bioenvironmental Engineering
March 12, 2015
Dr. John Pardue, Louisiana State Univ.
(Biogeochemistry/Biodegradation)
Dr. Donna Fennell, Rutgers University
(Microbial Characterization, Dioxin Dechlorination)
Dr. Danny Reible, Texas Tech Univ.
(Sediment Remediation/Bioavailability)
Dr. Lily Young, Rutgers University
(PAH Degradation)
Dr. John Reinfelder, Rutgers Univ.
(Hg Chemistry)
Dr. Andrew Jackson, Texas Tech University
(Dialysis sampling of porewater)
Dr. William Moe, Louisiana State University
(Chloroflexi Bacteria Isolation, Bioremediation)
March 12, 2015
What is Project in-ECO?
In-Situ Bioremediation 101
Project in-ECO Pilot Study
Pilot Study Activities: 2014
Pilot Study Forecast: 2015-2017
in-ECO PossibilitiesMarch 12, 2015
Innovative Ideas CollaborationSustainable
SolutionsMarch 12, 2015
A 21st CENTURY
SOLUTION
NEEDEDInitially an Effort to
Identify Current State of
Remediation Science
RECENT SCIENCE
ADVANCESOver the Past 10 Years,
Promising Sediment Bioremediation
Advances
PILOT STUDY
Field Test Laboratory Advances
Project in-ECO – IdeaField Test Recent Scientific Advances
in Sediment Bioremediation
March 12, 2015
The in-ECO Pilot Study Designed to Demonstrate
in-situ Sediment Bioremediation as a Viable 21st
Century Sustainable Remediation Technology for
Passaic River & Newark Bay Sediments
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In-situ bioremediation is the useof microorganisms to degrade
and detoxify chemicals in theenvironment “in place” without
the need to excavate and movethe contaminated material.
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In-situ bioremediation uses two strategies to degrade
contaminants
Stimulating Bacteria by Adding
Nutrients or Food Sources
Adding Certain Types of Bacteria
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HH
Cl-
Bacteria, nutrients,H2
Tetrachlorodibenzodioxin
More toxicTrichlorodibenzodioxin
Less toxic
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Ecologically Friendly
• Minimal Disruption of Ecology
and Habitats
• In-situ Destruction of Contaminants
• Limited Need for Treatment
Facilities or Landfills
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Climate Change Friendly
• Low Greenhouse Gas Emissions
• Low Environmental Impact
• Low Carbon Footprint
• EPA Region 2 Clean and Green
policy encourages selection of
sustainable technologies
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Society Friendly
• No Commuter Disruption
• Minimal Heavy Truck Traffic
• Minimal Recreation Use Impacts
• No Odor
• No Noise
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� Bacterial group that degrades
dioxins is now known
(Dehalococcoides)
� Tools available to track bacteria
� Years of experience with bacteria
in the field (groundwater sites)
� We understand what makes
these bacteria grow and thriveMarch 12, 2015
�Need to know whether we can stimulate the bacteria in Passaic River/Newark Bay sediments
�Can we uniformly inject amendments in the sediment?
�Promise of the Michigan lab studies from late 1990’s
�Key science questions in the field
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March 12, 2015
LPR1LPR1
NB1NB1
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Which carbon
amendments are
suitable for
application at the
study sites?
H
Are lower chlorinated dioxins
stable indicators of biodegradation
over the short-term (months)?
Sediment Sampling Bacteria Testing Special samplers
March 12, 2015
EPA Review
EPA Review
Mar Apr May Jun Jul Aug Sep
Dec. 19, 2014Pre-Design Work Plan Submitted
to EPA
March 20, 2014Advised EPA of
interest to pursue in-situ Bioremediation
Study
Oct Nov Dec
2014
March-May May-June July – August September-November
Pilot Study
Work Plan
November-Present
Revised Pilot
Study
Work Plan
Pilot Study
Statement
of Work
May 15, 2014Submitted Pilot
Study Statement of Work to EPA
July 1, 2014Submitted Pilot Study Work Plan
to EPA
Sept. 2, 2014EPA Review Comments
Received
Nov. 20, 2014Submitted Revised
Pilot Study Work Plan to EPA
2015
Jan
March 12, 2015
Feb Mar
March 2015EPA/DEP
Comments on Pre-Design Work
Plan
Design & Contracting Pilot Study Final
Report
Jan Mar May Jul Sep Nov
2016
Jan Mar May
March 2015Re-Submission of Pilot Study Work
Plan to EPA
April – July 2015Pre-Design Studies
Jul Sep Nov
20172015
2016Interim Pilot Study Report
2017Final Pilot
Study Report
March-July 2015 July-August 2015 August 2015 – May 2017 May-July 2017
Caisson Fab. &
Installation
Jan Mar Jul
June 2015NJDEP/EPA Permitting Completed
EPA Review/Approval Pilot Study Work Plan
EPA Review
Pre-Design
Plan
March 12, 2015
Faster Remedy
• Innovative Remedy Could Be
Implemented Sooner
• Lower Remedy Costs Could
Facilitate Better Cooperation
March 12, 2015
Job Creation and Education
• Good Jobs
• Transferable Skills
• Higher Learning/Skill
Opportunities
• Direct Involvement of Local
Universities and Colleges
• New Industry of High-Tech
Research Jobs March 12, 2015
Passaic Leadership
• International Model for 21st Century
Urban Waterway Clean-Ups
• Business Opportunities
• Enhanced Image
March 12, 2015
SUPPORT A BETTER SOLUTION
SUPPORT PROJECT in-ECO
THANK YOUMarch 12, 2015
1) “Production and Dechlorination of 2,3,7,8-Tetrachlorodibenzo-p-dioxinin Historically- Contaminated Estuarine Sediments”; I.D. Albrecht, A.L. Barkovskii, and P. Adriaens, January 1999
2) “Reductive dehalogenation of chlorinated dioxins by an anaerobic bacterium”; Michael Bunge, Lorenz Adrian, Angelika Kraus, Matthias Opel,
Wilhelm G. Lorenz, Jan R. Andreesen, Helmut Gorisch and Ute Lechner, January 2003
3) “Role of “Dehalococcoides” spp. in the Anaerobic Transformation of Hexachlorobenzene in European Rivers”; NeslihanTas, Miriam H. A. van Eekert, Anke Wagner, Gosse Schraa,Willem M. de Vos,and Hauke Smidt, July 2011
4) “Quantifying Enhanced Microbial Dehalogenation Impacting the Fate and Transport of Organohalide Mixtures in Contaminated Sediments”; Max Haggblom, Donna Fennell, Lisa Rodenburg, Lee Kerkhof, Kevin Sowers, February 2012
5) “Dechlorination of Commercial PCBs and Other Multiple Halogenated Compounds by a Sediment-Free Culture Containing Dehalococcoides and Dehalobacter”; Shanquan Wang and Jianzhong He, August 2013
6) “Genomic Characterization of Three Unique Dehalococcoides that Respire on Persistent Polychlorinated Biphenyls”; Shanquan Wang, Kern Rei Chng, Andreas Wilm, Siyan Zhao , Kun-Lin Yang Niranjan Nagarajan and Jianzhong He, August 2014
7) “Dehalococcoides mccartyi Strain DCMB5 Respires a Broad Spectrum of Chlorinated Aromatic Compounds”; Marlén Pöritz, Christian L. Schiffmann, Gerd Hause, Ulrike Heinemann, Jana Seifert, Nico Jehmlich, Martin von Bergen, Ivonne Nijenhuis, Ute Lechner, January 2015
Recent Scientific Advances – Reference Materials
March 12, 2015
Clean & Green PolicyEPA Region 2
Selected EPA Green Policy
Website LinksPrinciples for Greener
CleanupsEPA HQ
EPA CLU-IN
EPA OSWER
EPA REGION 2
March 12, 2015
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SUPPORT A BETTER SOLUTION
SUPPORT PROJECT in-ECO
THANK YOU
March 12, 2015