bioshare: omics in healthy obesity project - marja-liisa nuotio
TRANSCRIPT
Omics in Healthy Obesity Project
An outline:• What is “omics”?• Metabolomics in Healthy Obesity Project• Genetics in Healthy Obesity Project• Future prospects
Omics
Genome
Transcriptome
Epigenome
GlycomeMetabolome
Phenome
Kettunen et al. Nat Gen 2012
NMR METABOLOME
Metabolic effects of MHO• Phenotype:1. Metabolically healthy obesity defined according to the less
strict criteria in HOP phase 1 (BMI>=30 kg/m2, Syst.Bp<=140 mmHg, Diast.Bp<90 mmHg, no medication for elevated bp./lipids, Gluc.<7.0 mmol/l, HDL>=1.03 mmol/l for men/ >=1.3 mmol/l for women, Trigly<1.7 mmol/l, no preval. diabetes, no preval. CVD, no diagnosis for hypertension) (case)
2. Metabolically unhealthy obesity (control)• Linear regression: metabolite ~ MHO + age + sex + fasting time (+ trigly)
Two independent Finnish cohorts
• The National FINRISK 1997 study- A survey on risk factors of chronic, noncommunicable diseases- Population-based, age and sex stratified sample- NMR metabolome available for 3997 individuals- Discovery
• The Young Finns Study- A population-based, 27-year follow-up study for cardiovascular risk
factors- NMR metabolome available for 2040 individuals- Replication
GWAS in Healthy Obesity Project• Logistic regression: MHO status ~ SNP + age + sex + other covariates- 1000 genomes reference panel
QC and revision of the summary statistics in an individual study level is currently ongoing.This will be followed by an inverse-variance weighted meta-analysis and replication.
Where are we heading to?Future prospects
Future for HOP omics• GWAS meta-analysis + replication
• NMR in other cohorts?
• Other omics?– Transcriptomics– Epigenomics
• BBMRI-LPC 3rd call!
BBMRI-LPC calls• A FP7 –I3 project offering help and funding in accessing large
prospective studies in Europe– The BBMRI-LPC project constitutes a large network of 22 European
cohort studies, involving over 1 million study participants with lifestyle information and biospecimens collected at baseline
• Works through scientific calls (with only light bureaucracy) where the scientifically best studies are selected for support and funding– Funding is available for sample and data revival and for GWAS &
metabolomics (latter more limited)
• The next (and last) call in fall 2015
• Should Bioshare/HOP-team apply for access for GWAS&metabolomic studies?
1st BBMRI-LPC
call
April-July 20142nd BBMRI-LPC
call
March-June 2015
3rd BBMRI-LPC
call
Coming up in fall
2015
BBMRI-LPC Calls
Acknowledgement
FINLAND:Anni JoensuuMarkus PerolaVeikko SalomaaTero HiekkalinnaThe rest of the group PerolaJohannes KettunenTHE NETHERLANDS:Bruce WolffenbuttelJana van Vliet-OstaptchoukChao PangHans Hillege
NORWAY:Kirsti KvaloyGERMANY:Melanie WaldenbergerEva ReischlESTONIA:Krista FischerEemil PartinenITALY:Luisa FocoUNITED KINGDOM:Christopher Newby
The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 261433 (Biobank Standardisation and Harmonisation for Research Excellence in the European Union - BioSHaRE-EU)