biotechnology activities in bangladeshris.org.in/images/ris_images/pdf/biotech-bangladesh.pdf ·...
TRANSCRIPT
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Bangladesh
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Biotechnology Activities in Bangladesh
An overview
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Presented by
• Dr. Syed Saleheen Qadri• Professor, Department of Biochemistry and
Molecular Biology, Dhaka University (DU)• Past Chairman, Department of Biochemistry
and Molecular Biology, DU• Former Dean, Faculty of Biological Sciences.• Former Director, Centre for Advanced
Research in Sciences, DU
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Geography
• Deltaic plain at the confluence of the Ganges, Brahmaputra, and Meghna Rivers and their tributaries
• Has a tropical monsoon climate characterized by heavy seasonal rainfall, high temperatures, and high humidity.
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Geography
• Deltaic plain at the confluence of the Ganges, Brahmaputra, and Meghna Rivers and their tributaries
• Has a tropical monsoon climate characterized by heavy seasonal rainfall, high temperatures, and high humidity.
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Geography
• Area of 144,000 square kilometers and extends 820 kilometers north to south and 600 kilometers east to west.
• Bordered on the west, north, and east by a 2,400-kilometer land frontier with India and, in the southeast, by a short land and water frontier (193 km) with Myanmar.
• Coastline 580 km
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Geography
• Area of 144,000 square kilometers and extends 820 kilometers north to south and 600 kilometers east to west.
• Bordered on the west, north, and east by a 2,400-kilometer land frontier with India and, in the southeast, by a short land and water frontier (193 km) with Myanmar.
• Coastline 580 km
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Geography
• Area of 144,000 square kilometers and extends 820 kilometers north to south and 600 kilometers east to west.
• Bordered on the west, north, and east by a 2,400-kilometer land frontier with India and, in the southeast, by a short land and water frontier (193 km) with Myanmar.
• Coastline 580 km
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Geography
• Roughly 80 % of the landmass is made up of fertile alluvial lowland called the Lower Gangetic Plain.
• 67% of nonurban land is arable.• Permanent crops cover only 3%, meadows
and pastures 4%, and forests and woodland cover 16%.
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Geography
• Roughly 80 % of the landmass is made up of fertile alluvial lowland called the Lower Gangetic Plain.
• Permanent crops cover only 3%, meadows and pastures 4%, and forests and woodland cover 16%.
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Geography
• Natural resources: natural gas, arable land, timber, coal
• Land use:Arable land: 55.39%Permanent crops: 3.08%other: 41.53% (2005)
• Irrigated land: 47,250 km² (2003)
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Geography
• Natural resources: natural gas, arable land, timber, coal
• Land use:Arable land: 55.39%Permanent crops: 3.08%other: 41.53% (2005)
• Irrigated land: 47,250 km² (2003)
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Geography
• Natural resources: natural gas, arable land, timber, coal
• Land use:Arable land: 55.39%Permanent crops: 3.08%other: 41.53% (2005)
• Irrigated land: 47,250 km² (2003)
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People & Economy
• Population (2009 est): 160 million (8th)• Population density: 1099.3/km2 (9th)• GDP per capita: USD 583• HDI: 0.543 (146th)
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Agriculture at a Glance
• Total family : 17,600,804 • Total farm holding : 15,089,000 • Total area : 14.845 million hectare • Forest : 2.599 million hectare • Cultivable land : 8.44 million hectare • Cultivable waste : 0.268 million hectare • Current fellow : 0.469 million hectare • Cropping intensity : 175.97%
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Agriculture at a Glance
• Total family : 17,600,804 • Total farm holding : 15,089,000 • Total area : 14.845 million hectare • Forest : 2.599 million hectare • Cultivable land : 8.44 million hectare • Cropping intensity : 175.97%
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Agriculture at a Glance
• Single cropped area : 2.851 million• Double cropped area : 3.984 million • Triple cropped area : 0.974 million • Net cropped area : 7.809 million • Total cropped area : 13.742 million(In hectares)
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Agriculture at a Glance
• Contribution of agriculture sector to GDP : 23.50% • Contribution of crop sector to GDP : 13.44% • Manpower in agriculture : 62% • Total food crop demand : 23.029 million metric ton • Total food crop production : 27.787 million metric ton • Net production : 24.569 million metric ton
(Source: BBS, 2006 and Handbook Agricultural Statistics, MoA)
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Major agricultural products
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Biotechnology ActivitiesGovernment of Bangladesh
• National Policy on Biotechnology adopted in 2006, now updated 2010
• National Biosafety Framework 2006• National Institute of Biotechnology• Support for jute genome sequencing
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Biotechnology ActivitiesGovernment of Bangladesh
• National Policy on Biotechnology adopted in 2006, now updated 2010
• National Biosafety Framework 2006• National Institute of Biotechnology• Support for jute genome sequencing
![Page 22: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/22.jpg)
Biotechnology ActivitiesGovernment of Bangladesh
• National Policy on Biotechnology adopted in 2006, now updated 2010
• National Biosafety Framework 2006• National Institute of Biotechnology• Support for jute genome sequencing
![Page 23: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/23.jpg)
Biotechnology ActivitiesGovernment of Bangladesh
• National Policy on Biotechnology adopted in 2006, now updated 2010
• National Biosafety Framework 2006• National Institute of Biotechnology• Support for jute genome sequencing
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National Biotechnology PolicyPriority areas
• Plant biotechnology• Animal biotechnology• Fish biotechnology• Medical biotechnology• Industrial biotechnology• Environmental biotechnology• Biodiversity conservation• Human genetics and genomics• Biosafety & bioethics
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National Biosafety Framework 2006
• National Biosafety Guidelines 2006 • National Committee on Biosafety• Institutional Committee on Biosafety• National Bioethics Committee• Institutional Bioethics Committee
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National Institute of Biotechnology (NIB)
• Cost: Tk 267.8 million• Total Floor space: 3700 sq m• Lab floor space: 2250 sq m• Number of labs: 06
DNA lab, Plant biotech, Animal biotech, Fish biotech, Fermentation & Bioprocessing , and Bioenergy & Fertilisation
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NIB objectives
• Research on priority problems• Networking of national projects• Human resource development• Policy planning• Technology Resource Centre
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NIB Facilities Available
• Plant tissue culture• Recombinant DNA technology• DNA sequencing including PCR• DNA fingerprinting & profiling• Enzyme technology & product analysis• Micro-element analysis of environmental
samples• Automated greenhouse, animal house
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NIB on going projects
• In vitro propagation of banana• Micropropagation of strawberry• Disease-free orchid production• Mass production of potato microtuber• Conservation of endangered medicinal plants• Regeneration and gene transformation
protocol for hyv rice
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NIB on going projects
• In vitro propagation of banana• Micropropagation of strawberry• Disease-free orchid production• Mass production of potato microtuber• Conservation of endangered medicinal plants• Regeneration and gene transformation
protocol for hyv rice
![Page 31: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/31.jpg)
NIB on going projects
• In vitro propagation of banana• Micropropagation of strawberry• Disease-free orchid production• Mass production of potato microtuber• Conservation of endangered medicinal plants• Regeneration and gene transformation
protocol for hyv rice
![Page 32: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/32.jpg)
NIB on going projects
• In vitro propagation of banana• Micropropagation of strawberry• Disease-free orchid production• Mass production of potato microtuber• Conservation of endangered medicinal plants• Regeneration and gene transformation
protocol for hyv rice
![Page 33: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/33.jpg)
NIB on going projects
• In vitro propagation of banana• Micropropagation of strawberry• Disease-free orchid production• Mass production of potato microtuber• Conservation of endangered medicinal plants• Regeneration and gene transformation
protocol for hyv rice
![Page 34: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/34.jpg)
NIB on going projects
• In vitro propagation of banana• Micropropagation of strawberry• Disease-free orchid production• Mass production of potato microtuber• Conservation of endangered medicinal plants• Regeneration and gene transformation
protocol for hyv rice
![Page 35: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/35.jpg)
Other Institutions Involved in Biotech Activities
• GOB research institutes• Public universities• NGOs• Private companies
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Bangladesh Agricultural Research Institute
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Bangladesh Agricultural Research Institute (BARI)
• At Joydebpur, 165 ha + 6 regional stations + 24 substations
• Biotechnology lab started in 1985• Well equipped modern laboratory• Facilities for genetic engineering and
molecular biology work• Recent addition a Class II greenhouse
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Bangladesh Agricultural Research Institute (BARI)
• At Joydebpur, 165 ha + 6 regional stations + 24 substations
• Biotechnology lab started in 1985• Well equipped modern laboratory• Facilities for genetic engineering and
molecular biology work• Recent addition a Class II greenhouse
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BARI Objectives in Biotechnology• Identify disease and pest resistance genes for cloning,
sequencing and expression• Identify genes to characterize plants, diagnose plant
diseases and pests, assay active components and gene mapping
• Develop transgenic plants• Develop new genotypes through somatic hybridization
and gene pyramiding• Introduce transgenics or somatic hybrids from abroad• Field trial of transgenics for risk assessment and field
release
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BARI Objectives in Biotechnology• Identify disease and pest resistance genes for cloning,
sequencing and expression• Identify genes to characterize plants, diagnose plant
diseases and pests, assay active components and gene mapping
• Develop transgenic plants• Develop new genotypes through somatic hybridization
and gene pyramiding• Introduce transgenics or somatic hybrids from abroad• Field trial of transgenics for risk assessment and field
release
![Page 41: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/41.jpg)
BARI Objectives in Biotechnology• Identify disease and pest resistance genes for cloning,
sequencing and expression• Identify genes to characterize plants, diagnose plant
diseases and pests, assay active components and gene mapping
• Develop transgenic plants• Develop new genotypes through somatic hybridization
and gene pyramiding• Introduce transgenics or somatic hybrids from abroad• Field trial of transgenics for risk assessment and field
release
![Page 42: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/42.jpg)
BARI Objectives in Biotechnology• Identify disease and pest resistance genes for cloning,
sequencing and expression• Identify genes to characterize plants, diagnose plant
diseases and pests, assay active components and gene mapping
• Develop transgenic plants• Develop new genotypes through somatic hybridization
and gene pyramiding• Introduce transgenics or somatic hybrids from abroad• Field trial of transgenics for risk assessment and field
release
![Page 43: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/43.jpg)
BARI Objectives in Biotechnology• Identify disease and pest resistance genes for cloning,
sequencing and expression• Identify genes to characterize plants, diagnose plant
diseases and pests, assay active components and gene mapping
• Develop transgenic plants• Develop new genotypes through somatic hybridization
and gene pyramiding• Introduce transgenics or somatic hybrids from abroad• Field trial of transgenics for risk assessment and field
release
![Page 44: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/44.jpg)
BARI Objectives• Identify disease and pest resistance genes for cloning,
sequencing and expression• Identify genes to characterize plants, diagnose plant
diseases and pests, assay active components and gene mapping
• Develop transgenic plants• Develop new genotypes through somatic hybridization
and gene pyramiding• Introduce transgenics or somatic hybrids from abroad• Field trial of transgenics for risk assessment and field
release
![Page 45: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/45.jpg)
BARI achievements in Biotechnology
• Tissue culture of banana, jackfruit, pineapple, water melon, papaya, coconut, grape, gladiolus, orchids, chrysanthemum, rose, tuberose, strawberry, gourd, ginger, brinjal, and potato
• Bioengineered crops: Bt brinjal (9 varieties) and Leaf Blight Resistant Potato (2 varieties)
• RAPD analysis to estimate genetic polymorphism in various plants
• Microsatellite DNA markers used for characterization of plants
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BARI achievements in Biotechnology
• Tissue culture of banana, jackfruit, pineapple, water melon, papaya, coconut, grape, gladiolus, orchids, chrysanthemum, rose, tuberose, strawberry, gourd, ginger, brinjal, and potato
• Bioengineered crops: Bt brinjal (9 varieties) and Leaf Blight Resistant Potato (2 varieties)
• RAPD analysis to estimate genetic polymorphism in various plants
• Microsatellite DNA markers used for characterization of plants
![Page 47: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/47.jpg)
BARI achievements in Biotechnology
• Tissue culture of banana, jackfruit, pineapple, water melon, papaya, coconut, grape, gladiolus, orchids, chrysanthemum, rose, tuberose, strawberry, gourd, ginger, brinjal, and potato
• Bioengineered crops: Bt brinjal (9 varieties) and Leaf Blight Resistant Potato (2 varieties)
• RAPD analysis to estimate genetic polymorphism in various plants
• Microsatellite DNA markers used for characterization of plants
![Page 48: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/48.jpg)
BARI achievements in Biotechnology
• Tissue culture of banana, jackfruit, pineapple, water melon, papaya, coconut, grape, gladiolus, orchids, chrysanthemum, rose, tuberose, strawberry, gourd, ginger, brinjal, and potato
• Bioengineered crops: Bt brinjal (9 varieties) and Leaf Blight Resistant Potato (2 varieties)
• RAPD analysis to estimate genetic polymorphism in various plants
• Microsatellite DNA markers used for characterization of plants
![Page 49: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/49.jpg)
Bangladesh Rice Research Institute (BRRI)
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Bangladesh Rice Research Institute (BRRI)
• Situated at Gazipur + 10 regional stations• Biotechnology work initiated in 1982• Major thrust: varietal development activities
for high yield, quality, stress tolerance and bio-fortification
• Involved in rice tissue culture, genetic transformation, marker assisted selection, gene pyramiding, QTL identification and DNA fingerprinting
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Bangladesh Rice Research Institute (BRRI)
• Situated at Gazipur + 10 regional stations• Biotechnology work initiated in 1982• Major thrust: varietal development activities
for high yield, quality, stress tolerance and bio-fortification
• Involved in rice tissue culture, genetic transformation, marker assisted selection, gene pyramiding, QTL identification and DNA fingerprinting
![Page 52: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/52.jpg)
Bangladesh Rice Research Institute (BRRI)
• Situated at Gazipur + 10 regional stations• Biotechnology work initiated in 1982• Major thrust: varietal development activities
for high yield, quality, stress tolerance and bio-fortification
• Involved in rice tissue culture, genetic transformation, marker assisted selection, gene pyramiding, QTL identification and DNA fingerprinting
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Bangladesh Rice Research Institute (BRRI)
• Situated at Gazipur + 10 regional stations• Biotechnology work initiated in 1982• Major thrust: varietal development activities
for high yield, quality, stress tolerance and bio-fortification
• Involved in rice tissue culture, genetic transformation, marker assisted selection, gene pyramiding, QTL identification and DNA fingerprinting
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BRRI Achievements in Biotechnology
• Methods and protocols established for culturing explants, such as seed, embryo, young panicle and anther of reluctant Indica rice
• Media for one-step regeneration in anther culture
• Genetically engineered submergence tolerant rice BRRIDhan51 and BRRIDhan52 ready to be released
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BRRI Achievements in Biotechnology
• Methods and protocols established for culturing explants, such as seed, embryo, young panicle and anther of reluctant Indicarice
• Media for one-step regeneration in anther culture
• Genetically engineered submergence tolerant rice BRRIDhan51 and BRRIDhan52 ready to be released
![Page 56: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/56.jpg)
BRRI Achievements in Biotechnology
• Methods and protocols established for culturing explants, suchas seed, embryo, young panicle and antherof reluctant Indica rice
• Media for one-step regeneration in anther culture
• Submergence tolerant rice BRRIDhan51 and BRRIDhan52 ready to be released
![Page 57: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/57.jpg)
Other GOB institutes involved in Biotechnolgy
• Bangladesh Council of Scientific and Industrial Research (BCSIR): tissue culture of Stevia, Bixa, Rawoulfia
• Atomic Energy Research Establishment: Plant Biotechnology and Genetic Engineering Division: tissue culture of forest, fruit, medicinal and ornamental plants
• Bangladesh Sugarcane Research Institute: tissue culture of sugarcane, DNA fingerprinting of sugarcane varieties, mushroom tissue culture using sugarcane bagasse, Agrobacterium mediated salt and drought tolerant sugarcane
![Page 58: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/58.jpg)
Other GOB institutes involved in Biotechnolgy
• Bangladesh Council of Scientific and Industrial Research (BCSIR): tissue culture of Stevia, Bixa, Rawoulfia
• Atomic Energy Research Establishment: Plant Biotechnology and Genetic Engineering Division: tissue culture of forest, fruit, medicinal and ornamental plants
• Bangladesh Sugarcane Research Institute: tissue culture of sugarcane, DNA fingerprinting of sugarcane varieties, mushroom tissue culture using sugarcane bagasse, Agrobacterium mediated salt and drought tolerant sugarcane
![Page 59: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/59.jpg)
Other GOB institutes involved in Biotechnolgy
• Bangladesh Council of Scientific and Industrial Research (BCSIR): tissue culture of Stevia, Bixa, Rawoulfia
• Atomic Energy Research Establishment: Plant Biotechnology and Genetic Engineering Division: tissue culture of forest, fruit, medicinal and ornamental plants
• Bangladesh Sugarcane Research Institute: tissue culture of sugarcane, DNA fingerprinting of sugarcane varieties, mushroom tissue culture using sugarcane bagasse, Agrobacterium mediated salt and drought tolerant sugarcane
![Page 60: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/60.jpg)
Public Universities
• Bangladesh Agricultural University (BAU)• Rajshahi University (RU)• Khulna University (KU)• Chittagong University (CU)• Dhaka University (DU)
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Department of Biotechnology, BAU
• Fungal disease resistance of local potato through gene transfer
• Agrobacterium tumefaciens mediated genetic transformations of potato, garlic and chilli
• Genetic engineering approaches for blight and streak resistance in rice
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Department of Biotechnology, BAU
• Fungal disease resistance of local potato through gene transfer
• Agrobacterium tumefaciens mediated genetic transformations of potato, garlic and chilli
• Genetic engineering approaches for blight and streak resistance in rice
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Department of Biotechnology, BAU
• Fungal disease resistance of local potato through gene transfer
• Agrobacterium tumefaciens mediated genetic transformations of potato, garlic and chilli
• Genetic engineering approaches for blight and streak resistance in rice
![Page 64: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/64.jpg)
Institute of Biological Sciences, RU
• Genetic transformation of antifungal genes into Dalbergia sissoo
• Molecular phylogeny studies on banana• Arsenic hyper accumulation by fern species• Biodiesel from Jatropha
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Institute of Biological Sciences, RU
• Genetic transformation of antifungal genes into Dalbergia sissoo
• Molecular phylogeny studies on banana• Arsenic hyper accumulation by fern species• Biodiesel from Jatropha
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Institute of Biological Sciences, RU
• Genetic transformation of antifungal genes into Dalbergia sissoo
• Molecular phylogeny studies on banana• Arsenic hyper accumulation by fern species• Biodiesel from Jatropha
![Page 67: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/67.jpg)
Institute of Biological Sciences, RU
• Genetic transformation of antifungal genes into Dalbergia sissoo
• Molecular phylogeny studies on banana• Arsenic hyper accumulation by fern species• Biodiesel from Jatropha
![Page 68: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/68.jpg)
Department of Botany, RU
• Strawberry: Development and release of somaclonal variant suitable for Bangladesh
• Potato: Virus-free seed, nutritionally improvement by genetic transformation, DNA fingerprinting
• Rice: genetic transformation with PRP genes• Lettuce: genetic transformation and
chromosomal mapping, transgenic expression of pesticidal gene, production of calcitonin in transgenic lettuce
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Department of Botany, RU
• Strawberry: Development and release of somaclonal variant suitable for Bangladesh
• Potato: Virus-free seed, nutritionally improvement by genetic transformation, DNA fingerprinting
• Rice: genetic transformation with PRP genes• Lettuce: genetic transformation and
chromosomal mapping, transgenic expression of pesticidal gene, production of calcitonin in transgenic lettuce
![Page 70: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/70.jpg)
Department of Botany, RU
• Strawberry: Development and release of somaclonal variant suitable for Bangladesh
• Potato: Virus-free seed, nutritionally improvement by genetic transformation, DNA fingerprinting
• Rice: genetic transformation with PRP genes• Lettuce: genetic transformation and
chromosomal mapping, transgenic expression of pesticidal gene, production of calcitonin in transgenic lettuce
![Page 71: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/71.jpg)
Department of Botany, RU
• Strawberry: Development and release of somaclonal variant suitable for Bangladesh
• Potato: Virus-free seed, nutritionally improvement by genetic transformation, DNA fingerprinting
• Rice: genetic transformation with PRP genes• Lettuce: genetic transformation and
chromosomal mapping, transgenic expression of pesticidal gene, production of calcitonin in transgenic lettuce
![Page 72: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/72.jpg)
Department of Botany, RU
• Tomato: QLT analysis with RFLP and RAPD• Micropropagation for mass production of:
water chestnut, water melon, orchids, banana, pineapple, horticultural and medicinal plants
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Department of Botany, RU
• Tomato: QLT analysis with RFLP and RAPD• Micropropagation for mass production of:
water chestnut, water melon, orchids, banana, pineapple, horticultural and medicinal plants
![Page 74: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/74.jpg)
Dhaka University
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Department of Botany, DU
• Development of suitable regeneration systems for Brassica spp, Gerberra, Tea, Rice, Chrysanthemum, Potato, Tomato, Peanut, Lentil, Chickpea, Mungbean and Brinjal
• Agrobacterium mediated transformations of above plants and jute for fungal resistance
• RAPD analyses in Chrysanthemum, Gerbera, Aromatic rice, Peanut.
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Department of Botany, DU
• Development of suitable regeneration systems for Brassica spp, Gerberra, Tea, Rice, Chrysanthemum, Potato, Tomato, Peanut, Lentil, Chickpea, Mungbean and Brinjal
• Agrobacterium mediated transformations of above plants and jute for fungal resistance
• RAPD analyses in Chrysanthemum, Gerbera, Aromatic rice, Peanut.
![Page 77: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/77.jpg)
Department of Botany, DU
• Development of suitable regeneration systems for Brassica spp, Gerberra, Tea, Rice, Chrysanthemum, Potato, Tomato, Peanut, Lentil, Chickpea, Mungbean and Brinjal
• Agrobacterium mediated transformations of above plants and jute for fungal resistance
• RAPD analyses in Chrysanthemum, Gerbera, Aromatic rice, Peanut.
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Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Marker-assisted backcrossing to produce salt tolerant versions of the mega rice varieties BR11 and BRRIdhan28. BR11-Saltol already provided to BRRI for multiplication
• Saltol-linked marker validation in breeding populations
• Search for salinity tolerance QTLs from Bangladesh landraces e.g Boilam
• Sequence analysis of waxy and red pericarp gene in Bangladesh landraces
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Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Marker-assisted backcrossing to produce salt tolerant versions of the mega rice varieties BR11 and BRRIdhan28. BR11-Saltol already provided to BRRI for multiplication
• Saltol-linked marker validation in breeding populations
• Search for salinity tolerance QTLs from Bangladesh landraces e.g Boilam
• Sequence analysis of waxy and red pericarp gene in Bangladesh landraces
![Page 80: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/80.jpg)
Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Marker-assisted backcrossing to produce salt tolerant versions of the mega rice varieties BR11 and BRRIdhan28. BR11-Saltol already provided to BRRI for multiplication
• Saltol-linked marker validation in breeding populations
• Search for salinity tolerance QTLs from Bangladesh landraces e.g Boilam
• Sequence analysis of waxy and red pericarp gene in Bangladesh landraces
![Page 81: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/81.jpg)
Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Marker-assisted backcrossing to produce salt tolerant versions of the mega rice varieties BR11 and BRRIdhan28. BR11-Saltol already provided to BRRI for multiplication
• Saltol-linked marker validation in breeding populations
• Search for salinity tolerance QTLs from Bangladesh landraces e.g Boilam
• Sequence analysis of waxy and red pericarp gene in Bangladesh landraces
![Page 82: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/82.jpg)
Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Isolation and characterization of salt-inducible promoters from salt-tolerant rice landraces
• Agrobacterium mediated transformation of rice for salt-tolerance
• Use of rice seed for delivery of recombinant cholera vaccine
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Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Isolation and characterization of salt-inducible promoters from salt-tolerant rice landraces
• Agrobacterium mediated transformation of rice for salt-tolerance
• Use of rice seed for delivery of recombinant cholera vaccine
![Page 84: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/84.jpg)
Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Isolation and characterization of salt-inducible promoters from salt-tolerant rice landraces
• Agrobacterium mediated transformation of rice for salt-tolerance
• Use of rice seed for delivery of recombinant cholera vaccine
![Page 85: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/85.jpg)
Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Identification of marker for low temperature tolerance in jute
• Expression of xyloglucan endotransglycosylase genes to confer fungi caused stem-rot resistance in jute
• Expression of bacterial kat E gene in jute for conferring salt tolerance
• SEQUENCING THE JUTE GENOME
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Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Identification of marker for low temperature tolerance in jute
• Expression of xyloglucan endotransglycosylase genes to confer fungi caused stem-rot resistance in jute
• Expression of bacterial kat E gene in jute for conferring salt tolerance
• SEQUENCING THE JUTE GENOME
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Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Identification of marker for low temperature tolerance in jute
• Expression of xyloglucan endotransglycosylase genes to confer fungi caused stem-rot resistance in jute
• Expression of bacterial kat E gene in jute for conferring salt tolerance
• SEQUENCING THE JUTE GENOME
![Page 88: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/88.jpg)
Department of Biochemistry and Molecular Biology, DU (BMBDU)
• Identification of marker for low temperature tolerance in jute
• Expression of xyloglucan endotransglycosylase genes to confer fungi caused stem-rot resistance in jute
• Expression of bacterial kat E gene in jute for conferring salt tolerance
• SEQUENCING THE JUTE GENOME
![Page 89: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/89.jpg)
Sequencing the Jute Genome• Biotechnologists at a meeting identified sequencing
the jute genome as a priority target for Bangladesh.• Prof. Maqsudul Alam, University of Hawaii, Manoa in
one of his home visits learned of this and offered to sequence the jute genome. But funds necessary were not forthcoming.
• At a subsequent meeting where he was describing his success with Papaya genome in Hawaii and Rubber genome in Malaysia, Minister for Agriculture, Begum Motia Chowdhury, who was present in the meeting became interested and promised to help find the funds.
![Page 90: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/90.jpg)
Sequencing the Jute Genome• Biotechnologists at a meeting identified sequencing
the jute genome as a priority target for Bangladesh.• Prof. Maqsudul Alam, University of Hawaii, Manoa in
one of his home visits learned of this and offered to sequence the jute genome. But funds necessary were not forthcoming.
• At a subsequent meeting where he was describing his success with Papaya genome in Hawaii and Rubber genome in Malaysia, Minister for Agriculture, Begum Motia Chowdhury, who was present in the meeting became interested and promised to help find the funds.
![Page 91: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/91.jpg)
Sequencing the Jute Genome• Biotechnologists at a meeting identified sequencing
the jute genome as a priority target for Bangladesh.• Prof. Maqsudul Alam, University of Hawaii, Manoa in
one of his home visits learned of this and offered to sequence the jute genome. But funds necessary were not forthcoming.
• At a subsequent meeting where he was describing his success with Papaya genome in Hawaii and Rubber genome in Malaysia, Minister for Agriculture, Begum Motia Chowdhury, who was present in the meeting became interested and promised to help find the funds.
![Page 92: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/92.jpg)
Sequencing the Jute Genome
• Soon, a remarkable event took place: GOB allocated Tk 100 million, and a journey Swapna Jatra began
• With Dr. Maqsudul Alam leading the way, DNA preparation and marker selections were done at the DUBMB lab (Prof Haseena Khan and her team), the wet lab work was done in Malaysia and Hawaii, the entire computer work was done at Dhaka by young boys and girls at a software firm, Dhaka Soft with help from MS students from DUBMB
![Page 93: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/93.jpg)
Sequencing the Jute Genome
• Soon, a remarkable event took place: GOB allocated Tk 100 million, and a journey Swapna Jatra began
• With Dr. Maqsudul Alam leading the way, DNA preparation and marker selections were done at the DUBMB lab (Prof Haseena Khan and her team), the wet lab work was done in Malaysia and Hawaii, the entire computer work was done at Dhaka by novices, young boys and girls at a software firm, Dhaka Soft with help from MS students from DUBMB
![Page 94: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/94.jpg)
Sequencing the Jute Genome
• On 16 June 2010, Prime Minister Sheikh Hasina proudly announced that Bangladeshi scientists had decoded the Jute Genome
• “The discovery of genome sequencing of jute would, she said, facilitate better pest control and better finished industrial products out of jute.” Sheikh Hasina said.
• Surely Bangladesh can deliver
![Page 95: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/95.jpg)
Sequencing the Jute Genome
• On 16 June 2010, Prime Minister Sheikh Hasina proudly announced that Bangladeshi scientists had decoded the Jute Genome
• “The discovery of genome sequencing of jute would, she said, facilitate better pest control and better finished industrial products out of jute.” Sheikh Hasina said.
• Surely Bangladesh can deliver
![Page 96: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/96.jpg)
Sequencing the Jute Genome
• On 16 June 2010, Prime Minister Sheikh Hasina proudly announced that Bangladeshi scientists had decoded the Jute Genome
• “The discovery of genome sequencing of jute would, she said, facilitate better pest control and better finished industrial products out of jute.” Sheikh Hasina said.
• Surely Bangladesh can deliver
![Page 97: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/97.jpg)
Other Public Universities
• Biotechnology & Engineering Discipline, Khulna University: RAPD based genetic diversity analyses in Pumpkin, Brinjal, Lemon and Aromatic Rice.
• Department of Botany, Chittagong University: Agrobacterium mediated transformations in potato, rare and endangered orchids and medicinal plants.
![Page 98: Biotechnology Activities in Bangladeshris.org.in/images/RIS_images/pdf/Biotech-Bangladesh.pdf · Biotechnology Activities Government of Bangladesh • National Policy on Biotechnology](https://reader030.vdocuments.net/reader030/viewer/2022040817/5e61aade6db37376b4543408/html5/thumbnails/98.jpg)
Other Public Universities
• Biotechnology & Engineering Discipline, Khulna University: RAPD based genetic diversity analyses in Pumpkin, Brinjal, Lemon and Aromatic Rice.
• Department of Botany, Chittagong University: Agrobacterium mediated transformations in potato, rare and endangered orchids and medicinal plants.
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Other Public Universities
• Biotechnology & Engineering Discipline, Khulna University: RAPD based genetic diversity analyses in Pumpkin, Brinjal, Lemon and Aromatic Rice.
• Department of Botany, Chittagong University: Agrobacterium mediated transformations in potato, rare and endangered orchids and medicinal plants.
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Non-Governmental Organisation (NGO)
• BRAC Plant Biotechnology Laboratory• Commercialization of virus-free potato plantlets
and seed potatoes• Production through tissue culture of four popular
imported flower species (anthurium, gerbers, carnation and orchids)
• Production en masse of fancy cacti• Disease free fruit plantlets (banana, jackfruit,
strawberry, sweet karamcha and lemon• Commercialization of Stevia plants
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Private Companies
• Alpha Biotech Ltd: Potato plantlets and seeds• SAARC Agro-Biotech: Potato plantlets and
seeds;• Square Agro Development and Processing Ltd:
Potato plantlets and seeds; orchids; strawberry saplings.
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Private Companies
• Lal Teer Seed Ltd: potato plantlets and seeds; late blight resistant potato, yellow vein mosaic virus resistant okra; transgenic Bt-Brinjal (in collaboration with MaHyCo and Cornell University, USA with funding under ABSPII)
• AHZ Biotech Ltd: Potato seeds• Aman Plant tissue Culture Lab: Potato seeds
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Animal Biotechnology?
• Very little activity• GOB owned Bangladesh Livestock Research
Institute (BLRI)• Bangladesh Agricultural University (BAU)• Incepta Ltd and Square Ltd• DU Centre for Advanced Research in Sciences
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BLRI
• Molecular characterization of native cattle• Embryo transfer technology• Multiple ovulation and artificial insemination• Diagnosis of viral diseases like avian influenza,
foot and mouth disease• Genetic improvements in tilapia and carp.• Stock discrimination in hilsa by RFLP
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BAU
• Animal tissue culture lab facilities• Molecular characterization of Dengue• Molecular characterization and diagnosis of
avian flu and swine flu
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Private Companies
• Incepta: recombinant human insulin, oral cholera vaccine (soon to come)
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Overall Status
• Mostly plant biotech• Again, primarily tissue culture techniques.• Some work using gene manipulation, again
primarily Agrobacterium mediated• Fund constraints main limiting for Molecular
Biology and Genetic Engineering research• Another constraint: recruitment
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Biotech Scientists
• Dhaka University BMB:– Prof Haseena Khan– Prof Zeba Islam Seraj
• Dhaka University Botany:– Prof Syed Hadiuzzaman– Prof Rakha Hari Sarker– Prof Md. Imdadul Hoque
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Biotech Scientists
• Rajshahi University Botany:– Prof A K M Rafiqul Islam– Prof M Monzur Hossain
• BAU Biotechnology:– Prof K M Nasiruddin– Prof Md. Shahidul Haque– Prof. Md. Aminul Islam
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Biotech Scientists
• Khulna University Botany:– Prof Md Raihan Ali– Prof S M Mahbubur Rahman
• BRRI Biotechnology Division:– Dr. Md. Shamsher Ali– Dr. Md. Enamul Hoque
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Biotech Scientists• BARI:
– Dr. Md. Al-Amin– Dr A S M Mahbubur Rahman Khan
• BSRI:– Dr. Md. Amzad Hossain
• Biotechnologist:Prof Ahmed Shamsul IslamDr. Nayyum Chowdhury
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Biotechnology holds promise
• Better Nutrition: More food, more yield, more nutritive, less loss
• Better Health: Cheaper medicines, more effective targeted medicines, cheaper vaccines
• Better Environment: Cleaner environment, green fuel, green bioremediation and green waste disposal
• Better Living: Less hunger, reduced child mortality, improved maternal health, combat diseases, sustainable environment
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How to achieve?
• Fund constraints limit our ability to take advantage of newer technologies
• But, we have a pool of young talented scientists ready and willing to deliver
• All we need is a helping hand, the kind of help that enabled us decode the jute genome
• Please give us access to the resources available to you and together we shall prosper.
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Thank you in anticipation of your cooperation
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Thank you in anticipation of your cooperation