biotechnology worksheet

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Practice exam questions for biotechnology including PCR, plasmids and restriction enzymes

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Yr 12 BiologyBiotechnology Exam Questions

Question 1The diagram below represents a length of DNA and its cutting sites for the restriction enzymes speI, EcoRI, BgI II and HindIII.

Incubation of this length of DNA in a tube containing:a) Spe I would result in three pieces of DNAb) HindIII would result in two pieces of DNAc) SpeI and EcoRI would result in five pieces of DNAd) BglII and HindIII would result in four pieces of DNA

Question 2The following diagram indicates the cutting sites of 3 different restriction enzymes on a particular bacterial plasmid.

If the plasmid was incubated with the restriction enzyme Eco RI the number of pieces of DNA would be:a) 2b) 3c) 4d) 7

Question 3Amplification of DNA in the polymerase chain reaction requires:a) Nucleotides of uracilb) DNA polymerasec) Amino acidsd) Ribose sugar

Question 4A restriction enzyme used in molecular biology will:a) Restrict the activity of other enzymesb) Restrict its activity when reactions are to be slowedc) Enhance the activity of repressor molecules in metabolic pathwaysd) Cut DNA at a particular sequence e) Restrict the degradation of a particular reaction product

Question 5A restriction enzyme cutting site may be present or absent in a particular 200kb region of human chromosome 1.

The DNA of a person heterozygous for this cutting site would have the following gel pattern after digestion with the enzyme.

Question 6A soil bacterium (Agrobacterium tumefaciens) infects roses and fruit trees, stunting their growth. A similar bacterium A. radiobacter was genetically modified to include a plasmid gene coding for an antibiotic lethal to A tumefaciens. A radiobacter has a gene giving resistance to this antibiotic. A transfer gene located on the plasmid enables insertion of the modified gene into A. radiobacter. Suspensions of genetically modified bacteria are applied to the soil around plants. For this treatment to be successful genetic modification of A. radiobacter would need to include:a) Removal of all plasmidsb) Removal of the antibiotic genec) Destruction of the antibiotic resistance gened) Removal of the plasmid transfer gene

Question 6The diagrams below depict an electrophoresis gel and a plasmid map. Which sample in the gel contains DNA from the plasmid shown and which restriction enzyme was used to cut the DNA?

a) Sample 1 XbaIb) Sample 2 HindIIIc) Sample 3 Hind IIId) Sample 4 EcoRIe) Sample 5 XbaI

Question 7In the figure below 3 different samples (1-3) of DNA have been analysed by gel electrophoresis. Sample 1 is DNA of a chromosome extracted from a human cell. Sample 2 is DNA extracted from a particular species of bacteria. Sample 3 is a DNA molecule that is known to be 200bp long.

Which of the following correctly match the DNA samples (1-3) with the correct lane (A, B or C) in which they were loaded?

Question 8A sample of DNA is cut at the positions indicated below. When the fragments are loaded and run on an electrophoresis gel, which lane has the pattern that you would expect?

a) 1b) 2c) 3d) 4e) 5Question 9A DNA fingerprint is obtained by amplifying parts of an organisms genetic material and separating the resulting DNA fragments by a process known as gel electrophoresis. DNA fingerprinting is now used for a variety of applications including ecological studies, crime scene investigations and paternity testing. A cattle breeder is interested in determining which of his 3 bulls is the father of a calf born to his prize cow. The diagram below shows the DNA fingerprints of the cow, her calf and the 3 bulls.

From the diagram above, which of the bulls could be the father of the calf?a) Bull 1b) Bull 2c) Bull 3d) Either bull 1 or bull 3e) Either bull 2 or bull 3

Question 10DNA fingerprinting is often used to confirm the identity of an individual. In a family a childs DNA must be derived from their parents, with approximately half the childs genetic material coming from each parent. The diagram below shows a small section of the gel electrophoresis from a DNA fingerprint analysis of a family. Which of the children is least likely to be the offspring of both parents?

a) Child 1b) Child 2c) Child 3d) None of the childrene) All of the childrenSHORT ANSWER QUESTIONS

2000 VCAA

2001 VCAA

2002 VCAA

2003 VCAA

2004 VCAAPart of Q 3

2006 VCAA

2007 VCAA

2008 VCAA

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