Broade n in g of barle y ge n e pool

Urszula P iechota 1*, Paweł Cz. Czembor 2, J erzy H. Czembor 1

1National Centre of Plant Genetic Resource - Polish GeneBank, Plant Breeding and Acclimatization Institute – National Research Institute IHAR-PIB Radzikow, 05-870 Blonie, Poland

2Plant Breeding and Genetics Department, Plant Breeding and Acclimatization Institute – National Research Institute IHAR-PIB Radzikow, 05-870 Blonie, Poland

*u.piechota@ihar.edu.pl

This work is suppor ted by programme: “Cr ea t ion of scien t i fic ba sis for biologica l im p r ovem en t a n d

p la n t gen et ic r esour ces p r ot ect ion a s sou r ce of in n ova t ion a nd su p p or t of su sta in a ble

a gr icu l t u r e a n d n a t ion a l food secur i t y” funded by Minist ry of Agr icu lture and Rura l Development Poland.

Project No. 3-2-00-0-02: “Broadening of bar ley gene pool”.

Th e 3 yea r s of Mu l t ia n n ua l P r ogr a m m e

fou n d ed by Min ist r y of Agr icu l t u r e

a n d R u r a l Develop m en t P ola n d

P r oject : Br oa d en in g of ba r ley gen e p ool

The resistance of modern barley cultivars (Hordeumvulgare, L.) has a narrow genetic background.

Powdery mildew, caused by Blumeria graminis f. sp.hordei, is the most ecomicaly important barley disease.

Landraces originate from regions with traditionalagriculture are rich but still low operated source of geneticdiversity.

Resistence to powdery mildew is the aim ofnumerous breeding and scientific programmes.

http://bankgenow.edu.pl/en/

Phenotypic tests for powdery mildew resistance of 4 000 landraces

Resistnt line selection

F2 mapping population

Identification of molecular markerslinked to resistant trait

selected lines with broad spectrum resistance for powdery mildew:i) Bgh255-3-3ii) Bgh569-3-3iii) Bgh5617-1-1iv) Bgh39408-3-5

Identifying new sources of resistanceand new molecular markers linked toresistance genes opens newdimensions for breeders and researchprogrammes

resistant line × susceptible cultivar:i) Bgh255-3-3 × Manchuriaii) Bgh569-3-3 × Manchuria

Fitopathological tests of F2 plants and F3 families .

Numerous of PCR markers based on SSRsand DArTSeq oligos (Diversity ArraysTechnology Pty. Ltd.) was used to verifylinkage with powdery mildewresistance.

Barley line Bgh255-3-3genetic map

Powdery mildew resistant gene islocated on

2HS chromosome

The nearest marker: DArT5258111– 3.2cM distance:

chr2H 3653981 to 3654025*

*Phisical location for Ensembl Plants release 36 http://plants.ensembl.org

Genetic map calculated in JoinMap 4.0 softwere(Kyazma B. V.)

Bgh255-3-3 and Bgh569-3-3response to diverse collection ofpowdery mildew pathotypes.

Compared with lines’ setcarried various resistant.

Bgh255-3-3 and Bgh569-3-3 lines are resistant toall of used pathotypes.

Over 70.000 accesions in longterm storage (2nd among European Union)

Over 20.000 unique items in Herbariumcollecion

Well equipped molecular labQualified staff with passion to biodiversity

Scores: 0, 1, 2- resistant, 3, 4- susceptible,according to Mains’s and Dietz’s 5-level scale (1930).